BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0406.p
         (200 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313970|ref|YP_003778062.1| 2-hydroxychromene-2-carboxylate...   409   e-116
gi|300310604|ref|YP_003774696.1| 2-hydroxychromene-2-carboxylate...   107   8e-25
gi|300309925|ref|YP_003774017.1| 2-hydroxychromene-2-carboxylate...    69   3e-13

>gi|300313970|ref|YP_003778062.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 200

 Score =  409 bits (1051), Expect = e-116,   Method: Compositional matrix adjust.
 Identities = 197/200 (98%), Positives = 199/200 (99%)

Query: 1   MSTPIDFYFDFSSPYGYFAAVEIDKLAAQHGRDVNWHPILLGPIFKALGTNSLVNIPVKG 60
           MS PIDFYFDFSSPYGYFAAVEIDKLAAQHGRDVNWHPILLGPIFKALGTNSLVNIPVKG
Sbjct: 1   MSNPIDFYFDFSSPYGYFAAVEIDKLAAQHGRDVNWHPILLGPIFKALGTNSLVNIPVKG 60

Query: 61  EYSRHDMERTARFHNIFYKAPSHFPIGTQVAARATLWVQQTQPSKAVELIKTLYSAYFTE 120
           EYSRHDMERTARFHNIFYKAPSHFPIGTQVAARATLWVQQTQP+KAVELIKTLYSAYFTE
Sbjct: 61  EYSRHDMERTARFHNIFYKAPSHFPIGTQVAARATLWVQQTQPAKAVELIKTLYSAYFTE 120

Query: 121 DIDISVVDNVLRIAADLGIDRNALQAALESPELKEQLRQSTEAAGQAGVFGSPFMIVDGE 180
           DIDISVVDNVLRIAADLGIDRNALQAALESPELK+QLRQSTEAAGQAGVFGSPFMIVDGE
Sbjct: 121 DIDISVVDNVLRIAADLGIDRNALQAALESPELKDQLRQSTEAAGQAGVFGSPFMIVDGE 180

Query: 181 QFWGFDRFPQLEALLKNGKI 200
           QFWGFDRFPQLEALLKNGKI
Sbjct: 181 QFWGFDRFPQLEALLKNGKI 200


>gi|300310604|ref|YP_003774696.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 206

 Score =  107 bits (266), Expect = 8e-25,   Method: Compositional matrix adjust.
 Identities = 62/186 (33%), Positives = 92/186 (49%), Gaps = 4/186 (2%)

Query: 5   IDFYFDFSSPYGYFAAVEIDKLAAQHGRDVNWHPILLGPIFKALG--TNSLVNIPVKGEY 62
           I+++FDF S Y Y + + I++ A Q G  V W P LLGP+F+ LG  T+  V    KG Y
Sbjct: 8   IEYWFDFGSNYSYLSTMRIEQAAQQAGVRVVWRPFLLGPVFQELGWSTSPFVLQKEKGAY 67

Query: 63  SRHDMERTARFHNIFYKAPSHFPIGTQVAARATLWVQQTQPSKAVELIKTLYSAYFTEDI 122
           +  DM R  R + + +  PS FP    +A R  L  +      +    + +    F ED 
Sbjct: 68  TWKDMTRQCRKYGLPWTMPSRFPRAAVLATRVALSFEDALWMSS--FCRLVMHLNFAEDR 125

Query: 123 DISVVDNVLRIAADLGIDRNALQAALESPELKEQLRQSTEAAGQAGVFGSPFMIVDGEQF 182
           DI  V+ +  +   LG+    + A  +S   + +LR+ T  A   G+FG+P   V  E F
Sbjct: 126 DIDSVETISEVLQALGLPFAEIIAEAQSEPNRARLREQTRQARTLGIFGAPTFFVGDEMF 185

Query: 183 WGFDRF 188
           WG DR 
Sbjct: 186 WGNDRL 191


>gi|300309925|ref|YP_003774017.1| 2-hydroxychromene-2-carboxylate
           isomerase [Herbaspirillum seropedicae SmR1]
          Length = 201

 Score = 68.9 bits (167), Expect = 3e-13,   Method: Compositional matrix adjust.
 Identities = 54/195 (27%), Positives = 88/195 (45%), Gaps = 3/195 (1%)

Query: 1   MSTPIDFYFDFSSPYGYFAAVEIDKLAAQHGRDVNWHPILLGPIFKALGTNSLVN-IPVK 59
           MS   +++F   SP+ Y        LA ++   +   P  L  IF   G   L    P +
Sbjct: 1   MSKVCEYFFAPHSPFAYLGHDRFVALADKYQVQILPKPFDLSKIFGVSGGLPLAKRAPQR 60

Query: 60  GEYSRHDMERTARF-HNIFYKAPSHFPIGTQVAARATLWVQQTQ-PSKAVELIKTLYSAY 117
             Y   ++ R + F        P+HFP+ +  AAR  +  Q     + A+ L   +  A 
Sbjct: 61  QAYRLKELARWSEFLGKPMILQPAHFPVNSDNAARLIIATQLAHGTAAALRLAGAVMRAV 120

Query: 118 FTEDIDISVVDNVLRIAADLGIDRNALQAALESPELKEQLRQSTEAAGQAGVFGSPFMIV 177
           + E  +I+  D ++ IA     D  AL  + E+  ++ +  + T+ A  A VFG+P+ I 
Sbjct: 121 WEEQKNIADPDTLVAIANVQEHDGAALLKSAETASVQAEFDRFTDEANAASVFGAPWFIY 180

Query: 178 DGEQFWGFDRFPQLE 192
           +GE +WG DR   LE
Sbjct: 181 EGEGYWGQDRLDFLE 195


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.137    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 623,065
Number of extensions: 23939
Number of successful extensions: 87
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 83
Number of HSP's successfully gapped: 3
Length of query: 200
Length of database: 1,619,870
Length adjustment: 81
Effective length of query: 119
Effective length of database: 1,236,335
Effective search space: 147123865
Effective search space used: 147123865
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 128 (53.9 bits)