BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0405.p (394 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 537 e-154 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 165 6e-42 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 130 1e-31 gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 125 7e-30 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 83 5e-17 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 537 bits (1384), Expect = e-154, Method: Compositional matrix adjust. Identities = 260/262 (99%), Positives = 261/262 (99%) Query: 1 MIHLPGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGIT 60 MIHLPGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGIT Sbjct: 1 MIHLPGLSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGIT 60 Query: 61 VSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRL 120 VSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRL Sbjct: 61 VSEEYGGAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRL 120 Query: 121 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL 180 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL Sbjct: 121 ISGDFIGALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDL 180 Query: 181 DVGARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV 240 + GARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV Sbjct: 181 EAGARGMTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNV 240 Query: 241 LMSGLDFERSVLSGGPLGIMQA 262 LMSGLDFERSVLSGGPLGIMQA Sbjct: 241 LMSGLDFERSVLSGGPLGIMQA 262 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 165 bits (418), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 82/247 (33%), Positives = 147/247 (59%) Query: 18 LREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGYLAHII 77 +R+A+ FA + P AA+ DR+ FP + K+L +LG +G+ V E++GGAG+ Y++ ++ Sbjct: 10 IRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVL 69 Query: 78 AMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAG 137 A+EEI+ + ++L ++ G+ QK ++L L G+ +G ++EP+ G Sbjct: 70 ALEEIAAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTG 129 Query: 138 SDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLDVGARGMTAFLVEKGYK 197 SD +++ RA++ GD ++LNG+K +IT+G A V +V+A TD G +G++ FLV Sbjct: 130 SDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVTDRSAGKKGISCFLVPCDTP 189 Query: 198 GFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPL 257 GF V + +K+G S T +++ +C VP +LG G G + +S L+ R ++ + Sbjct: 190 GFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLGKEGEGYRIALSNLEAGRIGIAAQSV 249 Query: 258 GIMQAAW 264 G+ +AA+ Sbjct: 250 GMARAAF 256 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 130 bits (328), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 85/240 (35%), Positives = 127/240 (52%), Gaps = 2/240 (0%) Query: 7 LSFDHGEDIAALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYG 66 + F+ +D A +++ FA E+AP AA+ D FP+D+ K G+LG G+ E G Sbjct: 1 MDFELNQDQLAFQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADG 60 Query: 67 GAGLGYLAHIIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDFI 126 G GL L + EE++RA S H N+ I G K ++ +L +G I Sbjct: 61 GLGLSRLDATVVFEELARACTSTAAYLTIH-NMVSWMIASWGQPSLKAQWCEQLAAGRKI 119 Query: 127 GALAMSEPNAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKTDLDVGARG 186 G+ ++EP +GSD S+K A + YVLNGSK +I+ DVLVV A+T + GARG Sbjct: 120 GSYCLTEPGSGSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTGGE-GARG 178 Query: 187 MTAFLVEKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLD 246 ++A +V G S +K K+G T + F + +VP +++LG G+G M GLD Sbjct: 179 VSAIVVPGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLD 238 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 125 bits (314), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 85/249 (34%), Positives = 138/249 (55%), Gaps = 9/249 (3%) Query: 16 AALREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGYLAH 75 A LR+AV + +ID + +P L G L + +EYGG+GLG Sbjct: 11 ADLRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEYGGSGLGLTEA 70 Query: 76 IIAMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDF-IGALAMSEP 134 + MEEI+R + G +G N+ + R+G+ EQK +YLPR+ SG+ + ++A++EP Sbjct: 71 SVIMEEINRTGGNSGACHGQMYNM--GTLLRHGSPEQKERYLPRIASGELRLQSMAVTEP 128 Query: 135 NAGSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKT----DLDVGARGMTAF 190 G+D +K A KKGDRYV+NG K+WI+ +D++++ A+T + + GM+ F Sbjct: 129 TTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSRKSEGMSIF 188 Query: 191 LVE-KGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFER 249 LV+ K G + + L M T EL F + E+P EN++G GRG ++ GL+ ER Sbjct: 189 LVDLKEAIGHGMEVR-PILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAER 247 Query: 250 SVLSGGPLG 258 ++++ +G Sbjct: 248 TLIAAECIG 256 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 82.8 bits (203), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 60/236 (25%), Positives = 113/236 (47%), Gaps = 24/236 (10%) Query: 47 LWKKLGDLGVLGITVSEEYGGAGLGYLAHIIAMEEISRA-----SASVGLSYGAHSNLCV 101 ++ + D G + + E +GG L L +A EE++ SA +G G H+ Sbjct: 49 FYQAMADAGFIKALLPESFGGTALSTLDFALAAEELTAVDVNVPSALLGTGLGLHA---- 104 Query: 102 NQIKRNGNEEQKRKYLPRLIS-GDFIGALAMSEPNAGSDV------VSMKLRADKKGDRY 154 + +G EQK+++LP I G + ALA +E G++ V ++ A +GD + Sbjct: 105 --VVHHGTPEQKQRFLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARLEGDEW 162 Query: 155 VLNGSKMWITNG-----PDADVLVVYAKTDLDVG-ARGMTAFLVEKGYKGFSVAQKLDKL 208 V+NG+K + TNG A + V +TD + + + +V G + LD + Sbjct: 163 VINGAKHYTTNGTGWDGKGAHLYSVICRTDPALPPQQSLAVIMVPGNAPGIEIVGLLDTM 222 Query: 209 GMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFERSVLSGGPLGIMQAAW 264 G R + + + F + VP N++G G G+++L + + +++ +G+M+ A+ Sbjct: 223 GHRATISPRVHFNNVRVPASNIVGKPGDGISMLEAAFSWTAALIGSACVGVMRTAF 278 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,098,263 Number of extensions: 42670 Number of successful extensions: 176 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 11 Length of query: 394 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 307 Effective length of database: 1,207,925 Effective search space: 370832975 Effective search space used: 370832975 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)