BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0399.p (314 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313964|ref|YP_003778056.1| LysR family transcription regul... 632 0.0 gi|300312645|ref|YP_003776737.1| LysR family transcription regul... 139 4e-34 gi|300310443|ref|YP_003774535.1| LysR family transcription regul... 132 5e-32 gi|300312460|ref|YP_003776552.1| LysR family transcription regul... 131 8e-32 gi|300309577|ref|YP_003773669.1| transcription regulator protein... 130 1e-31 >gi|300313964|ref|YP_003778056.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 314 Score = 632 bits (1631), Expect = 0.0, Method: Compositional matrix adjust. Identities = 311/314 (99%), Positives = 312/314 (99%) Query: 1 MNKLPPLTALRAFEATARLLSFTAAADELHVTQSAVSRQVRILEAHLEQKLFVRLTRRIE 60 MNKLPPLTALRAFEATARLLSFTAAADELHVTQSAVSRQVRILEAHLEQKLFVRLTRRIE Sbjct: 1 MNKLPPLTALRAFEATARLLSFTAAADELHVTQSAVSRQVRILEAHLEQKLFVRLTRRIE 60 Query: 61 LTRAGAAYFQQIRQAFERICAAGEQLHARRQRHTLTIGVLPGLASLWLVPRLELFWRENK 120 LTRAGAAYFQQIRQAFERICAAGEQLHARRQRHTLTIGVLPGLASLWLVPRLELFWRENK Sbjct: 61 LTRAGAAYFQQIRQAFERICAAGEQLHARRQRHTLTIGVLPGLASLWLVPRLELFWRENK 120 Query: 121 EIDVRIVVATGAADFGRTTMDMSIQVGNLPGTTSQGLPLKKRMAVDWHDLRADLLFPDAL 180 EIDVRIVVATGAADFGRTTMDMSIQVGNLPGTTSQGLPLKKRMAVDWHDLRADLLFPDAL Sbjct: 121 EIDVRIVVATGAADFGRTTMDMSIQVGNLPGTTSQGLPLKKRMAVDWHDLRADLLFPDAL 180 Query: 181 VALCHPRLWRQHPVRSVADLSQHKLIHTAIRPDAWAHWLQQHGSRLGARQAGMECSQYPV 240 VALCHPRLWRQHPVRSVADLSQH+LIHTAIRPDAWAHWLQQHGSRLGARQAGMECSQYPV Sbjct: 181 VALCHPRLWRQHPVRSVADLSQHRLIHTAIRPDAWAHWLQQHGSRLGARQAGMECSQYPV 240 Query: 241 ALQAAMNGEGIALVPSVLAESFDAQHRLRRLFDGPTPSAGQYYLLTPGDRYDDSAVRRFR 300 ALQAAMNGEGIALVPSVLAESFD QHRLRRLF GPTPSAGQYYLLTPGDRYDDSAVRRFR Sbjct: 241 ALQAAMNGEGIALVPSVLAESFDPQHRLRRLFGGPTPSAGQYYLLTPGDRYDDSAVRRFR 300 Query: 301 QWLLMQAQAMGSTG 314 QWLLMQAQAMGSTG Sbjct: 301 QWLLMQAQAMGSTG 314 >gi|300312645|ref|YP_003776737.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 287 Score = 139 bits (349), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 107/305 (35%), Positives = 148/305 (48%), Gaps = 21/305 (6%) Query: 6 PLTALRAFEATARLLSFTAAADELHVTQSAVSRQVRILEAHLEQKLFVRLTRRIELTRAG 65 PL ALRAFE +AR LSFT AA+EL VTQ+AVS QV+ LE L LF RL R + LT G Sbjct: 4 PLNALRAFEVSARHLSFTRAAEELSVTQTAVSMQVKNLEQRLGVALFRRLPRGLALTDEG 63 Query: 66 AAYFQQIRQAFERICAAGEQLHARRQRHTLTIGVLPGLASLWLVPRLELFWRENKEIDVR 125 A + Q+F RI Q R+R LT+GV+ A WL+PRL F +++ +D+R Sbjct: 64 LALLPVVAQSFGRIADVLAQFEDGRRREVLTVGVVGTFAVGWLMPRLRQFQQQHPFVDLR 123 Query: 126 IVVATGAADFGRTTMDMSIQVGNLPGTTSQGLPLKKRMAVDWHDLRADLLFPDALVALCH 185 ++ D +D +I+ G+ WH +A+ LF L +C Sbjct: 124 LLTNNNRVDLAGEGLDYAIRFGD----------------GAWHGTQAERLFEAPLAPMCA 167 Query: 186 PRLWRQHPVRSVADLSQHKLIHTAIRPDAWAHWLQQHGSRLGARQAGMECSQYPVALQAA 245 P + + +RS ADL L+ + R D W W G+ + G +AA Sbjct: 168 PAIAAR--LRSPADLGAETLLRS-YRSDEWGRWFAAAGAACPPLR-GFVFDSSLALAEAA 223 Query: 246 MNGEGIALVPSVLAESFDAQHRLRRLFDGPTPSAGQYYLLTPGDRYDDSAVRRFRQWLLM 305 G+AL+P ++ E Q RL F G G Y+L + R +A+ FRQWL Sbjct: 224 AQEAGVALLPVLMFERELQQGRLVCPF-GQRIVLGAYWLTSLKSRPQTAAMAAFRQWLQS 282 Query: 306 QAQAM 310 A AM Sbjct: 283 HAGAM 287 >gi|300310443|ref|YP_003774535.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 295 Score = 132 bits (331), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 103/314 (32%), Positives = 148/314 (47%), Gaps = 22/314 (7%) Query: 1 MNKLPPLTALRAFEATARLLSFTAAADELHVTQSAVSRQVRILEAHLEQKLFVRLTRRIE 60 M LPPL L+AFEA AR SF AA ELH+T SAVS QV LE++L +LF R + Sbjct: 1 MAVLPPLPNLQAFEAVARRKSFALAAAELHLTASAVSHQVARLESYLGIRLFERSAHGVR 60 Query: 61 LTRAGAAYFQQIRQAFERICAAGEQLHARRQ--RHTLTIGVLPGLASLWLVPRLELFWRE 118 +T AG AY +I+ A I AA + RQ +TL I P +ASLWL+ RL F R Sbjct: 61 ITTAGEAYLARIQGAISAITAASMDV---RQGVSNTLYIHSAPSIASLWLMARLNDFARS 117 Query: 119 NKEIDVRIVVATGAADFGRTTMDMSIQVGNLPGTTSQGLPLKKRMAVDWHDLRADLLFPD 178 EI + A ++F +D+ I+ G +P +W L + LF + Sbjct: 118 YPEITFNLSAAHSQSNFELGEVDIDIRYG-IP---------------NWPGLVVEPLFEE 161 Query: 179 ALVALCHPRLWRQHPVRSVADLSQHKLIHTAIRPDAWAHWLQQHGSRLGARQAGMECSQY 238 +V L P R++ +R ADL KLI + + WA W + Q + + Sbjct: 162 RIVPLASPAFIREYRLRKPADLLGVKLIRSNVSVVQWADWFGEFADVRAPEQFAVRFDRA 221 Query: 239 PVALQAAMNGEGIALVPSVLAESFDAQHRLRRLFD-GPTPSAGQYYLLTPGDRYDDSAVR 297 ++L AA G G+AL + +A + +L+ +F ++ + P AV Sbjct: 222 QMSLDAATQGLGVALESATVAARHLEEKKLKPIFGLERAIKVKAHFAVYPAHHARRPAVA 281 Query: 298 RFRQWLLMQAQAMG 311 F +WL QA G Sbjct: 282 AFLEWLREQADGQG 295 >gi|300312460|ref|YP_003776552.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 312 Score = 131 bits (329), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 94/308 (30%), Positives = 142/308 (46%), Gaps = 15/308 (4%) Query: 2 NKLPPLTALRAFEATARLLSFTAAADELHVTQSAVSRQVRILEAHLEQKLFVRLTRRIEL 61 + LPPL AL+ FEA R S T AA EL +T SAVS+Q++ LE + EQ LF+R +R +E Sbjct: 3 SSLPPLKALKVFEAAVRTRSLTLAAHELFITHSAVSQQIKSLEEYFEQPLFLRQSRGVEP 62 Query: 62 TRAGAAYFQQIRQAFERICAAGEQLHARRQRHTLTIGVLPGLASLWLVPRLELFWRENKE 121 T A +++ +++ + +RI A EQL + + + P +A WL+PRL F +N Sbjct: 63 TEAALSFYAEVKASLDRIALAAEQLALAGKSRVVRLIAAPSVAMRWLIPRLSQFQIDNPR 122 Query: 122 IDVRIVVATGAADFGRTTMDMSIQVGNLPGTTSQGLPLKKRMAVDWHDLRADLLFPDALV 181 I+VR+ TTM + P + R ++ D V Sbjct: 123 IEVRVT----------TTMSEYFEDVKDPFDV-----IIWRSPLERSGYECMRFLDDVSV 167 Query: 182 ALCHPRLWRQHPVRSVADLSQHKLIHTAIRPDAWAHWLQQHGSRLGARQAGMECSQYPVA 241 L P HP+R D + LIH + R AW+ WL + G + + G ++ Sbjct: 168 PLASPHFLETHPIRVPRDFVEVPLIHLSSRSAAWSQWLSKAGVAVRRKMPGTVYEHSFLS 227 Query: 242 LQAAMNGEGIALVPSVLAESFDAQHRLRRLFDGPTPSAGQYYLLTPGDRYDDSAVRRFRQ 301 LQAA +G+ + L + A RL RLF Y++L + A++ F Sbjct: 228 LQAAATDQGVGMGTLALVDDDLAAGRLVRLFPEIVLHGEGYHMLCRSGAETNPALKTFIT 287 Query: 302 WLLMQAQA 309 WL QA Sbjct: 288 WLKQMGQA 295 >gi|300309577|ref|YP_003773669.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 301 Score = 130 bits (327), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 97/312 (31%), Positives = 148/312 (47%), Gaps = 19/312 (6%) Query: 1 MNKLPPLTALRAFEATARLLSFTAAADELHVTQSAVSRQVRILEAHLEQKLFVRLTRRIE 60 ++ LPPL +L AFEA R SFT AA ELH+TQSA+SRQ+ LE L +KLF+R R + Sbjct: 3 ISPLPPLPSLIAFEAAMRHGSFTRAAAELHLTQSAISRQIAQLERFLGKKLFLREPRALR 62 Query: 61 LTRAGAAYFQQIRQAFERICAAGEQLHARRQRHTLTIGVLPGLASLWLVPRLELFWRENK 120 LT +G Y +++ A E + R+ LT+ G+A LWL PRL F + Sbjct: 63 LTVSGQRYAALVQRLLVDCAEATEDIMKRKGHQELTVACSQGVAVLWLTPRLARFRARHP 122 Query: 121 EIDVRIVVATGAADFGRTTMDMSIQVGNLPGTTSQGLPLKKRMAVDWHDLRADLLFPDAL 180 + +R+VV A T DM I L GL ++ L+ + + Sbjct: 123 DCHLRLVVNDSLAQLSETDFDMGIYF--LRDGPPAGLAARR-------------LYDEEV 167 Query: 181 VALCHPRLWRQHPVRSVADLSQHKLIHTAIRPD---AWAHWLQQHGSRLGARQAGMECSQ 237 +C P + + + DL L+ +W +W+ +G + + +Q Sbjct: 168 FPVCSPG-YLDGRLLAPEDLRHQTLLTLEDGQRQWMSWQNWMAHNGMPDVRIEHQILSNQ 226 Query: 238 YPVALQAAMNGEGIALVPSVLAESFDAQHRLRRLFDGPTPSAGQYYLLTPGDRYDDSAVR 297 YPV LQ AM G+G+ L + ++ Q L R G YY + P DR + +A R Sbjct: 227 YPVLLQLAMEGQGVVLAWRHMIDACMEQGLLVRACQASASLGGGYYAVWPRDRIEHAAAR 286 Query: 298 RFRQWLLMQAQA 309 FR WL+ ++++ Sbjct: 287 AFRSWLVAESES 298 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,045,391 Number of extensions: 44872 Number of successful extensions: 305 Number of sequences better than 1.0e-08: 38 Number of HSP's gapped: 292 Number of HSP's successfully gapped: 39 Length of query: 314 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 229 Effective length of database: 1,217,395 Effective search space: 278783455 Effective search space used: 278783455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)