BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0378.p (495 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 944 0.0 gi|300310266|ref|YP_003774358.1| amidase family protein [Herbasp... 168 1e-42 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 133 4e-32 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 129 6e-31 gi|300309358|ref|YP_003773450.1| amidase family protein [Herbasp... 126 4e-30 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 944 bits (2441), Expect = 0.0, Method: Compositional matrix adjust. Identities = 483/495 (97%), Positives = 483/495 (97%), Gaps = 7/495 (1%) Query: 1 MHKLTLKELSAQLQARKISATELATLFLDRIDASDLNAFLHVDRELTLAQAKVADSRLSD 60 MHKLTLKELSAQLQARKISATELATLFLDRIDASDLNAFLHVDRELTLAQAKVADSRLSD Sbjct: 1 MHKLTLKELSAQLQARKISATELATLFLDRIDASDLNAFLHVDRELTLAQAKVADSRLSD 60 Query: 61 NNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNCD 120 NNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNCD Sbjct: 61 NNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNCD 120 Query: 121 EFAMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVXRRARRLLGRFGPAATATDTGGS 180 EFAMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAV R AATATDTGGS Sbjct: 121 EFAMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVAARLAP-------AATATDTGGS 173 Query: 181 IRQPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDS 240 IRQPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDS Sbjct: 174 IRQPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDS 233 Query: 241 TSLQRPKEDFTRSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEIS 300 TSLQRPKEDFTRSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEIS Sbjct: 234 TSLQRPKEDFTRSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEIS 293 Query: 301 LPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRR 360 LPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRR Sbjct: 294 LPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRR 353 Query: 361 ILVGSYVLSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTVAWDLGDK 420 ILVGSYVLSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTVAWDLGDK Sbjct: 354 ILVGSYVLSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTVAWDLGDK 413 Query: 421 TNDPVANYLADIFTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLNVA 480 TNDPVANYLADIFTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLNVA Sbjct: 414 TNDPVANYLADIFTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLNVA 473 Query: 481 HQFQQATDWHLREPA 495 HQFQQATDWHLREPA Sbjct: 474 HQFQQATDWHLREPA 488 >gi|300310266|ref|YP_003774358.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 168 bits (425), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 149/512 (29%), Positives = 231/512 (45%), Gaps = 49/512 (9%) Query: 6 LKELSAQLQARKISATELATLFLDRIDASDLNAFLHVD---RELTLAQ------------ 50 LKEL++ L + TE A+ +LD + AS +A+ +D E+ + Sbjct: 11 LKELASSLHIQL--TTEQASQYLDLMQAS-FDAYDVIDGLPDEIPAVKYPRSAAARPAAK 67 Query: 51 -----AKVADSRLSDNNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQ 105 A S + G+L G + KD G GS L+ +V +DAT+V + Sbjct: 68 DNPLNAWACKSTVKGAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGFVPSYDATIVTR 127 Query: 106 FNAAGMVTLGKLNCDEFAMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVXRRARRLL 165 AG LGK C+ F + G+ S P NP GGSS GSAA V + Sbjct: 128 LLDAGATILGKATCEHFCLSGGSHTSDPAPVHNPRRHGYGSGGSSSGSAALVAAGEADM- 186 Query: 166 GRFGPAATATDTGGSIRQPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDC 225 A D GGSIR P++ CG G+K T+G V G++ +++D GPI D Sbjct: 187 ------AIGGDQGGSIRIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDN 240 Query: 226 GLLLNAMTGFDERDSTSLQRPKEDFTRSLNDSLQGLRIGVPKEFFGAG-LAADVEAAVRA 284 L+L + G D D + +T +L+ ++GL+IG+ E F G + V V+ Sbjct: 241 ALMLEVIAGADGLDPRQYAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQE 300 Query: 285 ALSEFEKLGAQLVEISLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAA-DYGNLDD 343 A+++ EKLGA + +S+ + L+ ++ I + + +G+ + + D G L Sbjct: 301 AIAQLEKLGASVATVSVEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQH 360 Query: 344 M-YRKSRAEGFGEEVKRRILVGSYVLSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQC 402 + + A+ +K + VG Y L+ Y +Y +AQ I R + ALA Sbjct: 361 HAHWREHADDLSATLKLCMFVGQYGLTR-YQGRFYAKAQNIARRARAGYDKALA----DF 415 Query: 403 DVIMGPVSPTVAWDLGDKTNDPVANYLADIF-----TLSTSLAGLPGMSIPCGFGQGEKN 457 D+++ P P +A L P+A Y+A F T + G P MSIPCG G Sbjct: 416 DLLVMPTVPIIAQPL-PAAGCPLAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDG--- 471 Query: 458 GKRPVGLQIIGNYFDEARLLNVAHQFQQATDW 489 PVGL ++G ++ EA L A F+ + DW Sbjct: 472 --LPVGLMLVGKHYAEATLYQAAAAFEASMDW 501 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 133 bits (334), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 153/501 (30%), Positives = 217/501 (43%), Gaps = 72/501 (14%) Query: 4 LTLKELSAQLQARKISATELATLFLDRIDAS-DLNAFLHVDRELTLAQAKVADSRLSDNN 62 +TL E+ L R+ S E + +A DLN +L D E +QA++ D + Sbjct: 6 MTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDPEQLRSQARMLDEQ---GG 62 Query: 63 AGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNCDEF 122 G L+G+PIA KD G +TAG+ L N V ATVVE AG + GK N E Sbjct: 63 HGPLSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALHAGKANMHEL 122 Query: 123 AMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVXRRARRLLGRFGPAATATDTGGSIR 182 A G N N FGP NP+D + GGSSGG A + R AA TDTGGS+R Sbjct: 123 AFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAP-------AAVGTDTGGSVR 175 Query: 183 QPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTS 242 PA+LCGV G++PT S++G++ + + D GP+A++ D LL M G RDS Sbjct: 176 IPAALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAG--SRDSAP 233 Query: 243 L-QRPKEDFTRSL--NDSLQGLRIGVPK-------EFFGAGLA-ADVEAAVRAALSEFEK 291 L P + N S QGL V + AG+ DV+ A LS Sbjct: 234 LLALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELVDVDLPTLAPLSH--- 290 Query: 292 LGAQLVEISLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAE 351 A I L + + +P Y + A D+ ++ AE Sbjct: 291 --AAGFAIVLHEFLIDLPAYLQASDAR------------------------DVIKRVLAE 324 Query: 352 GFGEEVKRRILVGSYVLSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSP 411 +V R I L + Y A + RR + +++ ++ D ++ P + Sbjct: 325 VGSPDVARII---QATLDQPVPEHAYRLALEQRRTMQALYRDCF--RSQGVDALIFPTTA 379 Query: 412 TVAWDLGDKTNDPVANYLADIFTLS--------TSLAGLPGMSIPCGFGQGEKNGKRPVG 463 A LG+ +D VA + T S+AGLP +SIP G + PVG Sbjct: 380 LTARPLGE--DDTVALRGERVPTFPAFARNADGASIAGLPSISIPVGLSE----YGLPVG 433 Query: 464 LQIIGNYFDEARLLNVAHQFQ 484 L++ G + RLL VA + Sbjct: 434 LELDGPEGSDRRLLQVAASLE 454 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 129 bits (324), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 139/492 (28%), Positives = 227/492 (46%), Gaps = 38/492 (7%) Query: 5 TLKELSAQLQARKISATELATLFLDRIDASD-----LNAFLHVDRELTLAQAKVADSRLS 59 ++ L + A + ++ L +L RI A D LNA + ++ + ++ R Sbjct: 53 SITHLQSAQDAGRATSRSLVLAYLARIRAYDQQGPSLNAIVTLNPKALEEADQLDRERRQ 112 Query: 60 DNNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNC 119 G L G+PI KD + T TT G+ L + DA V++ AG V LGK Sbjct: 113 SGPRGPLHGIPILVKDNYDTVDMPTTGGTLALATLQAQADAFQVKRLREAGAVILGKTTM 172 Query: 120 DEFAMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVXRRARRLLGRFGPAATATDTGG 179 E A G +S G T NP+D R PGGSSGG+ AAV A +DT G Sbjct: 173 HELAAGVTTVSSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAA-------AGMGSDTCG 225 Query: 180 SIRQPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERD 239 SIR PA+ + G++ T G SR G++ +S+ D P+A++ ED ++L+A G D +D Sbjct: 226 SIRIPAAHQNLFGLRTTRGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDATVGSDPQD 285 Query: 240 STSL----QRPKEDFTRSLNDSLQGLRIGVPKEFFGAGLA-ADVEAAVRAALSEFEKLGA 294 S+++ PK DSLQG RIGV + FGA A+V AA+ AL + + GA Sbjct: 286 SSTVDANGHIPKSYRDGLRADSLQGARIGVLRALFGAAPEDAEVSAAINKALQQLKDQGA 345 Query: 295 QLVEISLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFG 354 + ++++P+ + + +I P E +L + H A G+L ++ A G Sbjct: 346 IVTDVTIPELDGLLSGSSII-PYEFKYDLGAY---LQSHPGAPVGSLGEIL----ARGME 397 Query: 355 EE-VKRRILVGSYVLSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTV 413 E ++ + + + V D L+ ++R + + N D ++ P Sbjct: 398 HELLEAGLRLRNSVDLQNPKDKEELEKVMLKRSALKSLMTDVMQKN-HLDTLVYPTIQRK 456 Query: 414 AWDLGDKTNDPVANYLADIFTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDE 473 A +G+ + L ++ GLP +++P GF + PV L+++ F E Sbjct: 457 AALIGEPQGGAMNCQL-------SATTGLPALALPVGFTE----DGLPVSLELLAPEFAE 505 Query: 474 ARLLNVAHQFQQ 485 LL +A+ ++Q Sbjct: 506 PALLGLAYGWEQ 517 >gi|300309358|ref|YP_003773450.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 126 bits (317), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 141/513 (27%), Positives = 218/513 (42%), Gaps = 59/513 (11%) Query: 1 MHKLTLKE---LSAQLQARKISATELATLFLDRIDASD--LNAFLHV-DRELTLAQAKVA 54 MH+L + LSA ++++++S E+ +L+ I+ + +NA + + E L +AK A Sbjct: 1 MHQLVTLDALALSAAIRSKEVSCREVMQAYLEHIEKMNPHVNAIVSLRPPEELLDEAKRA 60 Query: 55 DSRLSDNN-AGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVT 113 D L+ G + G+P A KD+ +G T+ GS + DAT V + AG + Sbjct: 61 DQLLAQGRYLGWMHGMPQAPKDLASCKGLPTSKGSPLSSMAPDQSDATCVARVRDAGAIF 120 Query: 114 LGKLNCDEFAMGSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVXRRARRLLGRFGPAAT 173 +GK N EF +GS + N FG T N +D + + G S A A R+L P A Sbjct: 121 IGKTNVSEFGLGSNSYNPLFGVTRNAYDFSRI---AGGSSGGAGAALAMRML----PVAD 173 Query: 174 ATDTGGSIRQPASLCGVTGIKPTYGSVSR-YGMIAFASSLDQGGPIAKTAEDCGLLLNAM 232 +D GS+R PA+ V G +PT G V G F L GP+ +T D L Sbjct: 174 GSDMMGSLRNPAAFNNVYGFRPTQGRVPYGSGGELFLQQLSTEGPMGRTVADVARLFATQ 233 Query: 233 TGFDERDSTSLQRPKEDFTRSLNDSLQGLRIGVPKEFFG-AGLAADVEAAVRAALSEFEK 291 G+D + S L +G RIG + G + +V AALS+F Sbjct: 234 AGYDPQCPLSSDTDVTGDAAQLKRDFKGARIGWLGNYDGYLPIEEEVMVLCEAALSDFAD 293 Query: 292 LGAQLVEISLPKTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAE 351 +G + E P Y + + HR G +Y Sbjct: 294 IGCTV--------EQCRPDY----------PMEKLWETWLVHRQWLVGGNLSVY------ 329 Query: 352 GFGEEVKRRILV--GSYVLSHGYYDAYYLQAQKIRR--LIAQDFQNALAGANRQCDVIMG 407 F + +R +L + + G YL A + R + D+ A+ Q D ++ Sbjct: 330 -FADPARRGLLKPEAQWEVESG----QYLTAADVFRASMARSDWYRAIHKLFEQFDYLLL 384 Query: 408 PVSPTVAWDLGDKTNDPVANYLADIF------TLSTSLAGLPGMSIPCGFGQGEKNGKRP 461 P + +D+ K VA D + + +LAGLP MS+P GFG NG P Sbjct: 385 PSAQMFPFDVATKWPRNVAGREMDTYHRWMEVVIGPTLAGLPAMSVPVGFG---ANGL-P 440 Query: 462 VGLQIIGNYFDEARLLNVAHQFQQATDWHLREP 494 +G+QIIG D+ +L +A+ + W + P Sbjct: 441 MGMQIIGPSRDDRGVLQLAYALEGLRPWSAQAP 473 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,417,313 Number of extensions: 57036 Number of successful extensions: 177 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 11 Length of query: 495 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 406 Effective length of database: 1,198,455 Effective search space: 486572730 Effective search space used: 486572730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)