BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0374.p (533 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 1048 0.0 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 501 e-143 gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis pro... 470 e-133 gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis pro... 446 e-126 gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis tra... 444 e-126 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 1048 bits (2710), Expect = 0.0, Method: Compositional matrix adjust. Identities = 533/533 (100%), Positives = 533/533 (100%) Query: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 Query: 61 KENGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKLR 120 KENGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKLR Sbjct: 61 KENGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKLR 120 Query: 121 AAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAIN 180 AAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAIN Sbjct: 121 AAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAIN 180 Query: 181 RDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSR 240 RDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSR Sbjct: 181 RDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSR 240 Query: 241 DETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEETA 300 DETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEETA Sbjct: 241 DETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEETA 300 Query: 301 SAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIADIIG 360 SAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIADIIG Sbjct: 301 SAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIADIIG 360 Query: 361 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVE 420 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVE Sbjct: 361 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVE 420 Query: 421 KVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQQ 480 KVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQQ Sbjct: 421 KVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQQ 480 Query: 481 NAALVEQAAAAARSMQDQAASLTDAVSVFRLEDDAQVLAVAVQRGTSPSTVVR 533 NAALVEQAAAAARSMQDQAASLTDAVSVFRLEDDAQVLAVAVQRGTSPSTVVR Sbjct: 481 NAALVEQAAAAARSMQDQAASLTDAVSVFRLEDDAQVLAVAVQRGTSPSTVVR 533 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 501 bits (1289), Expect = e-143, Method: Compositional matrix adjust. Identities = 292/511 (57%), Positives = 361/511 (70%) Query: 3 NLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAIKE 62 N+ IG RL +GF +L ++ +G+WRL + AT +M LK ER W+ + Sbjct: 6 NMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWYTNLVA 65 Query: 63 NGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKLRAA 122 RT A+ KS D A YF ++ A S + QK+VEA ++ EEK LF ++G+LR Sbjct: 66 GIRRTIAIAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLSTPEEKALFQKIGELRKL 125 Query: 123 YRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAINRD 182 Y ++R I K AG+ A + D V Y D + + DFQ++ +D TA I+ Sbjct: 126 YLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTAREIDAI 185 Query: 183 YRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRIDSSSRDE 242 R+++L L + ++LG A LT IT+PL++AV ++ VA GDL+S I +SRDE Sbjct: 186 AAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGDLSSEIAVTSRDE 245 Query: 243 TGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEETASA 302 TG+LL +LK MN +L IV VR GT+TI+TAS EIA GNLDLS+RTE QA SL ETASA Sbjct: 246 TGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSLAETASA 305 Query: 303 MEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIADIIGVI 362 ME+L STV+QNADNARQA++LA SAS VA QGG VV QVV TMGAI+ASS+KI DII VI Sbjct: 306 MEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRKIVDIISVI 365 Query: 363 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEKV 422 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSASAAKEIK+LI+DSV KV Sbjct: 366 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKELINDSVGKV 425 Query: 423 DDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQQNA 482 DDGSRLV +AG+TMQEVV SV RVT +V EI+ AS EQ+ GIEQ+NLAIT +D+ TQQNA Sbjct: 426 DDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQMDQVTQQNA 485 Query: 483 ALVEQAAAAARSMQDQAASLTDAVSVFRLED 513 ALVE+AAAAA SMQ QA L VSVFRL + Sbjct: 486 ALVEEAAAAAASMQTQAGRLMQMVSVFRLSE 516 >gi|300310594|ref|YP_003774686.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 541 Score = 470 bits (1209), Expect = e-133, Method: Compositional matrix adjust. Identities = 303/512 (59%), Positives = 362/512 (70%), Gaps = 3/512 (0%) Query: 3 NLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAIKE 62 N+KIG RLA+GF +L +V+VAL +WRL I ATD+M L KER W+ I Sbjct: 6 NMKIGRRLALGFAVILAAASVVVALSIWRLQGIAAATDEMMHEPLTKERLVSDWYRTIHT 65 Query: 63 NGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGKLRAA 122 + RT A+ KS D + A+F A S ++ QK +EA I +EK +F ++G++R Sbjct: 66 SVRRTTAIAKSSDPSLAAFFADDAAAASRMSTEQQKAIEALIESDQEKAVFTKLGEVRKD 125 Query: 123 YRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTAAAINRD 182 Y R +I K G A ++ Y D + + Q+ IDQ AA I Sbjct: 126 YIKYRDAISKAKAEGRTEEAAQILAGPFDVAGKGYLDLLQQLLTLQRANIDQVAAHIQTV 185 Query: 183 YRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRID---SSS 239 YR R+++LAL +++VLG A L ITRPL QAV +AE VAAGDLT RID ++ Sbjct: 186 YRESRNLMLALAFLQVVLGWLFARTLALGITRPLEQAVEVAEAVAAGDLTHRIDERLAAR 245 Query: 240 RDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSLEET 299 DETGKL+ AL M GNL IVG+VR GT+ I TAS+EIATGNLDLSARTE QAG+LEET Sbjct: 246 GDETGKLMHALHAMTGNLARIVGQVRGGTDAIATASKEIATGNLDLSARTERQAGALEET 305 Query: 300 ASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIADII 359 ASAME+LTSTV QNADNARQAN+LAASAS VA QGG VV QV++TMG+I+ SS+KI DII Sbjct: 306 ASAMEELTSTVSQNADNARQANQLAASASSVAQQGGEVVSQVISTMGSINESSRKIVDII 365 Query: 360 GVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSV 419 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIK LIDDSV Sbjct: 366 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKALIDDSV 425 Query: 420 EKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDETTQ 479 KV GS LV RAG TM EVV SV +V +V EI+ AS EQSDGI QVNLAIT +D+TTQ Sbjct: 426 AKVGSGSELVTRAGSTMDEVVASVRQVADIVSEISAASSEQSDGISQVNLAITDMDQTTQ 485 Query: 480 QNAALVEQAAAAARSMQDQAASLTDAVSVFRL 511 QNAALVE+AAAAA +MQ+QAA L++ VS F+L Sbjct: 486 QNAALVEEAAAAAEAMQEQAARLSEVVSQFKL 517 >gi|300310693|ref|YP_003774785.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 576 Score = 446 bits (1148), Expect = e-126, Method: Compositional matrix adjust. Identities = 287/532 (53%), Positives = 368/532 (69%), Gaps = 12/532 (2%) Query: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRIALKKERDAVQWHAAI 60 + ++KI VRL F AL+++M V+ +GV L + DATD++ K Q + Sbjct: 4 ISDMKISVRLGACFAALIVVMCVIAGVGVKNLLMVSDATDEIVNDRYVKVALGTQITDKV 63 Query: 61 KENGVRTF--AVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFDEVGK 118 G R+ A+M + + + Y + +D ++++S+ E+E L + + Sbjct: 64 N-TGARSLRNAIMARNPEEQKKYLDR-MDTYTSQVSEHLAEMEKRTNTPRGSELLAALKQ 121 Query: 119 LRAAYRATRQSIFDV--KTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQTA 176 RA Y R + D+ + D+A A + D T + + D Y V FQ ++D++A Sbjct: 122 ARADYSKERIKVVDLIHQQKRDEATA-HLFDKT-IPVQDKYFAAVKEMVSFQSSLMDKSA 179 Query: 177 AAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSRID 236 + S +++L L + ++L A A L+TRSITRPLN+AV +A VAAGDLT +I+ Sbjct: 180 ERASAASSSAINIMLVLSGVAVLLSALCAMLITRSITRPLNEAVQVATAVAAGDLTVQIE 239 Query: 237 SSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAGSL 296 ++S+DETG LL ALK+MN NL IV VR GT+TINTASREIATGNLDLS+RTE QAG+L Sbjct: 240 ATSKDETGALLGALKSMNDNLHRIVAEVRAGTDTINTASREIATGNLDLSSRTEQQAGAL 299 Query: 297 EETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQKIA 356 EETASAME+LTSTV+QNADNARQAN LA +ASQVA QGG+VV +VV TMG I+ +S+KI Sbjct: 300 EETASAMEELTSTVRQNADNARQANTLAETASQVAIQGGSVVGEVVQTMGQINDASRKIV 359 Query: 357 DIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLID 416 DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSA+AAKEIK LID Sbjct: 360 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAAAAKEIKSLID 419 Query: 417 DSVEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHIDE 476 SVE+V+ GSRLV +AG TM+EVV SV RVT VV EIT AS EQSDGIEQ+N AI +DE Sbjct: 420 ASVERVESGSRLVGQAGATMEEVVASVKRVTDVVAEITAASGEQSDGIEQINQAIVQMDE 479 Query: 477 TTQQNAALVEQAAAAARSMQDQAASLTDAVSVFRLEDD----AQVLAVAVQR 524 TQQNAALVEQAAAAA+S+Q+Q+ L+D V +FRL +D L+ VQR Sbjct: 480 VTQQNAALVEQAAAAAQSLQEQSGRLSDTVGIFRLGNDHAHQPSPLSAPVQR 531 >gi|300313820|ref|YP_003777912.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 444 bits (1143), Expect = e-126, Method: Compositional matrix adjust. Identities = 285/517 (55%), Positives = 367/517 (70%), Gaps = 12/517 (2%) Query: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDAT-----DDMTRIALKKE-RDAV 54 +K++KI VRLA+ F L+ +M ++ +G+ L SI ++ D +IAL E RD V Sbjct: 3 LKDIKISVRLAVCFAVLIGVMCLITGVGMKSLSSISKSSALIVEDRYEKIALAVEIRDNV 62 Query: 55 QWHAAIKENGVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLFD 114 A N A++ + + Y + A SA+ ++ ++E ++ A + LF Sbjct: 63 NGAARNLRN-----ALLGRNLEEATRYLDRSA-ANSAKTTEALGKMEKILSTARGRELFQ 116 Query: 115 EVGKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVIDQ 174 + + R+AY R + ++ A E+ T ++ D Y + ++ FQK +++Q Sbjct: 117 SIQEARSAYNTPRDKLRELIKQQKKEEAVELLFTQVIPAQDKYFQVLDQFVAFQKSLMEQ 176 Query: 175 TAAAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTSR 234 + +S +M+L L A+ I L AWL+TRSITRPLN+AV++A VAAGDLT + Sbjct: 177 SVTEGRDTTQSAIAMMLGLSAVAIALCVIAAWLVTRSITRPLNEAVSVASAVAAGDLTVQ 236 Query: 235 IDSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQAG 294 I ++S+DETG LLAALK+MN +L IV VR G++TINTAS EIATGNLDLS+RTE QAG Sbjct: 237 IGATSKDETGALLAALKSMNDSLHRIVSEVRQGSDTINTASAEIATGNLDLSSRTEEQAG 296 Query: 295 SLEETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQK 354 +LEETASAME+LTSTVKQNADNARQAN LAA+AS+VA QGG+VV QVV TMG I+ +S+K Sbjct: 297 ALEETASAMEELTSTVKQNADNARQANSLAATASEVAVQGGSVVGQVVQTMGEINDASRK 356 Query: 355 IADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQL 414 I DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR+LAQRSASAAKEIK L Sbjct: 357 IVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKAL 416 Query: 415 IDDSVEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITHI 474 IDDSV +VD+GSRLV +AG TM EVV SV RVT VV EI+ AS EQSDGIEQ+N AIT + Sbjct: 417 IDDSVSRVDNGSRLVEQAGTTMTEVVASVRRVTDVVAEISAASNEQSDGIEQINHAITQM 476 Query: 475 DETTQQNAALVEQAAAAARSMQDQAASLTDAVSVFRL 511 D+ TQQNAALVE+AAAAA+S+Q+Q+ L+ VSVF+L Sbjct: 477 DQVTQQNAALVEEAAAAAQSLQEQSTRLSATVSVFKL 513 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.126 0.327 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,160,731 Number of extensions: 39029 Number of successful extensions: 553 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 332 Number of HSP's successfully gapped: 70 Length of query: 533 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 443 Effective length of database: 1,193,720 Effective search space: 528817960 Effective search space used: 528817960 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)