BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0372.p (419 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum ... 863 0.0 gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase... 189 3e-49 gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidoh... 159 4e-40 >gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 863 bits (2231), Expect = 0.0, Method: Compositional matrix adjust. Identities = 416/419 (99%), Positives = 418/419 (99%) Query: 1 MDMNTQISTLRIDGARLWDSLMELARIGATAKGGVCRLALTDLDKQGRDLVSAWARAEGM 60 MDMNTQISTLRIDGARLWDSLMELARIGATAKGGVCRLALTDLDKQGRDLVS+WARAEGM Sbjct: 1 MDMNTQISTLRIDGARLWDSLMELARIGATAKGGVCRLALTDLDKQGRDLVSSWARAEGM 60 Query: 61 SVVVDQIGNIFMRRPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVLAGIEVVRTLNQHNI 120 SVVVDQIGNIFMRRPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVLAGIEVVRTLNQHNI Sbjct: 61 SVVVDQIGNIFMRRPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVLAGIEVVRTLNQHNI 120 Query: 121 QTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTLDHAYNATDVEGKKVRDELERIGYIG 180 QTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTLDHAYNATDVEGKKVRDELERIGYIG Sbjct: 121 QTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTLDHAYNATDVEGKKVRDELERIGYIG 180 Query: 181 DQVCGDHPIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEI 240 +QVCGDHPIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEI Sbjct: 181 EQVCGDHPIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEI 240 Query: 241 RKDALQVSTYIMQEVVAIAHRYAPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEVS 300 RKDALQVSTYIMQEVVAIAHRYAPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSE S Sbjct: 241 RKDALQVSTYIMQEVVAIAHRYAPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEAS 300 Query: 301 LTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHPDCVDKVRAASAALGYKTMDVVS 360 LTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHPDCVDKVRAASAALGYKTMDVVS Sbjct: 301 LTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHPDCVDKVRAASAALGYKTMDVVS 360 Query: 361 GAGHDAIYAARLAPAGMIFVPCKDGVSHNEIEDARPEHLEAGCNVLLQAMLASAGVVSA 419 GAGHDAIYAARLAPAGMIFVPCKDGVSHNEIEDARPEHLEAGCNVLLQAMLASAGVVSA Sbjct: 361 GAGHDAIYAARLAPAGMIFVPCKDGVSHNEIEDARPEHLEAGCNVLLQAMLASAGVVSA 419 >gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase/N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 587 Score = 189 bits (481), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 133/393 (33%), Positives = 201/393 (51%), Gaps = 17/393 (4%) Query: 27 IGATAK--GGVCRLALTDLDKQGRDLVSAWARAEGMSVVVDQIGNIFMRRPGRNNALPPV 84 IGA ++ G + LT+ ++ ++ W R GM V +D +GN+ R + + Sbjct: 190 IGALSEEEGALTCTYLTETHQRTAAQIAQWMREAGMQVHIDAVGNVVGRYQSPHPQAKTL 249 Query: 85 MTGSHIDTQPTGGKFDGNYGVLAGIEVVRTLNQHNIQTEAPIEVAVWTNEEGSRFVPVMM 144 +TGSH DT GGK+DG G+L I VV+ L++ +EV ++ EEG RF + Sbjct: 250 LTGSHYDTVRNGGKYDGREGILLPIAVVKYLHERGETLPFHLEVIGFSEEEGVRFKSTFL 309 Query: 145 GSGVFCGAFTLDHAYNATDVEGKKVRDELERIGY---IGDQVCGD-HPIGAYYEAHIEQG 200 GS G F + + D E +R+ + + G+ Q+ D I AY E HIEQG Sbjct: 310 GSNAITGHFDMA-LLDLRDKEDVTMRELIIQAGHDVAAIPQIARDPAEILAYVEVHIEQG 368 Query: 201 PVLENADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEIRKDAL----QVSTYIMQEVV 256 PVL D +G+V ++ G S Y + G SHAG TPM++RKDA ++ Y+ Q Sbjct: 369 PVLLKRDLPVGIVTSIAGSSRYLVDLKGVASHAGTTPMDMRKDAAAAAAEIILYVEQRCS 428 Query: 257 AIAHRYAPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVS-EVSLTRMHEDLLAYLEAM 315 H GTVGQ+QV S NVIPG + S+D+R + EV L + +D+LA +E + Sbjct: 429 QDQHAALV---GTVGQLQVPRGSTNVIPGACQLSLDIRAATDEVRLAAV-KDILAKIEEI 484 Query: 316 RKKTGLSIELERVSYFPPCPFHPDCVDKVRAASAALGYKTMDVVSGAGHDAIYAARLAPA 375 + + ++LE P P P + ++ AA+ G ++ SGAGHDA+ RL Sbjct: 485 CTRRSIDLKLEETLSAPAAPCAPWLMAQLSAATQRAGVPPFELASGAGHDAMAMVRLTDV 544 Query: 376 GMIFVPCKD-GVSHNEIEDARPEHLEAGCNVLL 407 M+F C + G+SHN +E + E +LL Sbjct: 545 CMLFTRCGNGGISHNPLETMTADDAEVSGQILL 577 >gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 159 bits (402), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 118/373 (31%), Positives = 176/373 (47%), Gaps = 21/373 (5%) Query: 33 GGVCRLALTDLDKQGRDLVSAWARAEGMSVVVDQIGNIFMR---RPGRNNALPPVMTGSH 89 G + R LT + ++ W GM V D GN+ R PG +A P +TGSH Sbjct: 30 GMLTRTYLTPQHQAAAAALARWMEEAGMRVRRDNAGNVIGRYEAAPGHESA-PSFVTGSH 88 Query: 90 IDTQPTGGKFDGNYGVLAGIEVVRTLNQHNIQTEAPIEVAVWTNEEGSRFVPVMMGSGVF 149 DT GG +DGN G++ + + ++ + PIEV + EEG RF ++GS Sbjct: 89 FDTVRNGGWYDGNLGIVLPLACIARWHRQGQRFAFPIEVIGFAEEEGVRFKATLLGSRTV 148 Query: 150 CGAFTLDHA-YNATDVEGKKVRDELERIGYIGDQVCGDH----PIGAYYEAHIEQGPVLE 204 G F D A D +G +R + G D + D + A+ E HIEQGP+L Sbjct: 149 AGTF--DQAVLENRDSDGVTMRAAIRAAGLDPDGIAADAWQPGSMAAFVEVHIEQGPLLL 206 Query: 205 NADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEIRKDAL----QVSTYIMQEVVAIAH 260 N D +GVV A+ G S + V G HAG PM +R+DA ++ YI + Sbjct: 207 NEDLPVGVVTAISGASRFMAEVHGLAGHAGTVPMHLRRDAAMAAAEIGLYIERRC----- 261 Query: 261 RYAPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEVSLTRMHEDLLAYLEAMRKKTG 320 P GT+G ++V+ + NV+PG FSID+R + D+ A ++ + + Sbjct: 262 SIKPELVGTMGLLEVVQGAANVVPGLASFSIDIRAEDDSDRLAAVADVKAEIDRIAARRQ 321 Query: 321 LSIELERVSYFPPCPFHPDCVDKVRAASAALGYKTMDVVSGAGHDAIYAARLAPAGMIFV 380 + + L + P P + + AA G+ + SGAGHDA+ A LA M+FV Sbjct: 322 VQLALRQTHEAASVPCAPRLQAALAQSIAAAGWPVRHLPSGAGHDAMALAGLADVAMLFV 381 Query: 381 PCKD-GVSHNEIE 392 C + G+SH+ E Sbjct: 382 RCGNGGISHHPDE 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,388,238 Number of extensions: 61354 Number of successful extensions: 173 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 3 Length of query: 419 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 331 Effective length of database: 1,203,190 Effective search space: 398255890 Effective search space used: 398255890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)