BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0366.p (497 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde deh... 999 0.0 gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde deh... 668 0.0 gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde deh... 463 e-131 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 208 9e-55 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 207 2e-54 >gi|300313930|ref|YP_003778022.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 497 Score = 999 bits (2583), Expect = 0.0, Method: Compositional matrix adjust. Identities = 491/497 (98%), Positives = 495/497 (99%) Query: 1 MEKIAHYIGGQRVDTKSGRYADVFNPAQGVPVAQVALGTSDEVDAAVRAGVAAFPAWSNT 60 MEKIAHYIGGQRVDT+SGRYADVFNPAQGVPVAQVALGTSDEVDAAV+AGVAAFPAWSNT Sbjct: 1 MEKIAHYIGGQRVDTRSGRYADVFNPAQGVPVAQVALGTSDEVDAAVKAGVAAFPAWSNT 60 Query: 61 PPLTRARVLFRFLHLIQQRADDIARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLK 120 PPLTRARVLFRFLHLIQQRADD ARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLK Sbjct: 61 PPLTRARVLFRFLHLIQQRADDFARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLK 120 Query: 121 GEYTDQIARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPS 180 GEYTDQIARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPS Sbjct: 121 GEYTDQIARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPS 180 Query: 181 ASLLIADLLKEAGLPDGVFNVLQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAK 240 ASLLIADLLKEAGLPDGVFNV+QGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAK Sbjct: 181 ASLLIADLLKEAGLPDGVFNVVQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAK 240 Query: 241 GKRVQALGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVAVAVGSAGDELVAA 300 GKRVQALGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVA+AVGSAGDELVAA Sbjct: 241 GKRVQALGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVALAVGSAGDELVAA 300 Query: 301 LAERAGKLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFEK 360 LAERA KLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFEK Sbjct: 301 LAERARKLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFEK 360 Query: 361 GFFVGGTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDGG 420 GFFVGGTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDGG Sbjct: 361 GFFVGGTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDGG 420 Query: 421 VAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKAVMQR 480 VAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKAVMQR Sbjct: 421 VAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKAVMQR 480 Query: 481 WPNTASAGAEFAFPQMK 497 WPNTASAGAEFAFPQMK Sbjct: 481 WPNTASAGAEFAFPQMK 497 >gi|300314075|ref|YP_003778167.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 503 Score = 668 bits (1724), Expect = 0.0, Method: Compositional matrix adjust. Identities = 334/501 (66%), Positives = 394/501 (78%), Gaps = 4/501 (0%) Query: 1 MEKIAHYIGGQRVDTKSGRYADVFNPAQGVPVAQVALGTSDEVDAAVRAGVAAFPAWSNT 60 + I H+I GQ ++SGR DVFNPA G A+VAL + EV+AAV A AA PAW+ T Sbjct: 3 LPHIDHFINGQISRSESGRSQDVFNPATGAATAKVALASVQEVNAAVAAASAAAPAWAET 62 Query: 61 PPLTRARVLFRFLHLIQQRADDIARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLK 120 PL RARVLF+F LI+Q D +A+ I EHGK FSDA+GEV RG+EIVEFA GIPQ+LK Sbjct: 63 APLKRARVLFKFKELIEQNHDALAKAITLEHGKVFSDAKGEVTRGLEIVEFATGIPQLLK 122 Query: 121 GEYTDQIARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPS 180 ++TD I GID W++RQ LGV AGITPFNFP MVP+WM PIAIA GN FVLKPSERDPS Sbjct: 123 TQFTDNIGGGIDNWNLRQPLGVTAGITPFNFPVMVPLWMAPIAIATGNSFVLKPSERDPS 182 Query: 181 ASLLIADLLKEAGLPDGVFNVLQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAK 240 SL++A+L K AGLPDGVFNV+QGDKV VDALL+HP+V+A+SFVGSTPIAEYIY + + + Sbjct: 183 PSLMLAELFKRAGLPDGVFNVVQGDKVAVDALLEHPEVQAVSFVGSTPIAEYIYRKATER 242 Query: 241 GK----RVQALGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVAVAVGSAGDE 296 R QALGGAKNH+VVMPDA++ AVDAL+GAAYGSAGERCMAISVAVAVGS D+ Sbjct: 243 AGTFPLRAQALGGAKNHLVVMPDANLDQAVDALIGAAYGSAGERCMAISVAVAVGSVADK 302 Query: 297 LVAALAERAGKLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVP 356 LV AL R LK+ GM AEMGP+VTAA K ++EG I GV+ GA L+VDGRG +VP Sbjct: 303 LVEALIPRVKALKVKNGMEDDAEMGPLVTAAHKAKVEGYIQSGVDAGAKLLVDGRGLKVP 362 Query: 357 GFEKGFFVGGTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYT 416 G E+GFF+GGTL DHVT EM +Y+EEIFGPVLCV+R PD ASAV+LINAHEYGNGV+++T Sbjct: 363 GHEEGFFIGGTLFDHVTTEMKIYQEEIFGPVLCVVRVPDFASAVQLINAHEYGNGVSLFT 422 Query: 417 RDGGVAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKA 476 DG A EF R+I+VGMVG+NVP+PVPMA+ SFGGWKRSLFGD H YG EG+RFYTR K+ Sbjct: 423 ADGNTAHEFSRRIQVGMVGINVPIPVPMAWHSFGGWKRSLFGDTHAYGEEGIRFYTRYKS 482 Query: 477 VMQRWPNTASAGAEFAFPQMK 497 +MQRW T GAEF P K Sbjct: 483 IMQRWSATIGKGAEFTMPVAK 503 >gi|300312371|ref|YP_003776463.1| methylmalonate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 463 bits (1191), Expect = e-131, Method: Compositional matrix adjust. Identities = 225/493 (45%), Positives = 318/493 (64%), Gaps = 3/493 (0%) Query: 7 YIGGQRVDTKSGRYADVFNPAQGVPVAQVALGTSDEVDAAVRAGVAAFPAWSNTPPLTRA 66 Y+ G++V + S + DV NPA VA+V T +EVD AV F W NT R Sbjct: 12 YLNGEKVQSTSKEWRDVLNPATQEVVARVPFATKEEVDRAVANAKETFKTWRNTSLAQRM 71 Query: 67 RVLFRFLHLIQQRADDIARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEYTDQ 126 R++ +F L+++ +A +I REHGKT DA+GEV RG+E+VE A I + GE + Sbjct: 72 RIMLKFQQLLRENIAPLAELITREHGKTLPDAEGEVMRGLEVVEHACSITSLQLGELAEN 131 Query: 127 IARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPSASLLIA 186 +A G+D +++ Q LGV AGIT FNFP M+P +MFPIA+ACGN FVLKPSE+DP++SL + Sbjct: 132 VAGGVDVYTLYQPLGVGAGITAFNFPVMLPCFMFPIAVACGNTFVLKPSEQDPTSSLFLV 191 Query: 187 DLLKEAGLPDGVFNVLQGDKVTVDALLDHPQVKAISFVGSTPIAEYIYARGSAKGKRVQA 246 +L +AGLP GV NV+ G + + DHP +KA+SF+GST + +IY R S GKR Q Sbjct: 192 ELANQAGLPPGVLNVVHGGPDVANMICDHPDIKAVSFIGSTHVGTHIYRRASEAGKRAQC 251 Query: 247 LGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVAVAVGSAGDELVAALAERAG 306 + GAKNH +V+PDA A++ L+GAA+G+AG+RCMA SV V VG E + + ER+ Sbjct: 252 MMGAKNHCIVLPDAPKDQAINNLLGAAFGAAGQRCMANSVVVLVGQT-REWIPEIVERSK 310 Query: 307 KLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFEKGFFVGG 366 +K+ G A++GP+V+ AAKER+E LI GVE+GA L++DGR +V G E G FVG Sbjct: 311 AMKVGPGTDRKADVGPLVSKAAKERVERLIASGVEQGAKLLLDGRNCKVAGSENGNFVGP 370 Query: 367 TLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDGGVAREFV 426 T+ V PEM +Y +EIFGP +C++ + A+ INA+ GNG +++T G AR+F Sbjct: 371 TVFTGVKPEMDIYTQEIFGPAMCIVELDTLDEAIAFINANPNGNGTSIFTSSGYAARKFQ 430 Query: 427 RQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKAVMQRW--PNT 484 +I+VG VG+NVP+PVP+A+ SF G + S GD G + V F+T+ K V RW P+ Sbjct: 431 NEIDVGQVGINVPIPVPVAYFSFTGSRASKLGDLGPNGKQAVTFWTQTKTVTARWYAPDE 490 Query: 485 ASAGAEFAFPQMK 497 + G P+++ Sbjct: 491 EAGGQHHHHPEVR 503 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 208 bits (529), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 164/479 (34%), Positives = 244/479 (50%), Gaps = 22/479 (4%) Query: 7 YIGGQRVDTKSGRYADVFNPAQGVPVAQVALGTSDEVDAAVRAGVAAFPAWSNTPPLTRA 66 YI G+ + A V PA V ++ALG + +VD AV+A A PAW+ T PL RA Sbjct: 19 YIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLERA 78 Query: 67 RVLFRFLHLIQQRADDIARIIVREHGKTFSDAQ-GEVARGIEIVEFAAGIPQMLKGEYTD 125 +L R L+ +R++ A+ + E G S A+ V E + A + +Y Sbjct: 79 ALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRVA----RDNLADYPF 134 Query: 126 QIARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPSASLLI 185 RG A +R+ +GV A ITP+N+P A+A G VLKPSE P ++LL Sbjct: 135 IRPRGTTAI-VREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSALLF 193 Query: 186 ADLLKEAGLPDGVFNVLQGDKVTVDALLD-HPQVKAISFVGSTPIAEYIYARGSAKGKRV 244 A+++ EAG+P GVFN++ G V A L HPQV IS GST + + KRV Sbjct: 194 AEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRV 253 Query: 245 -QALGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMA----ISVAVAVGSAGDELVA 299 Q LGG K+ +V+PDAD++ A+ + AA + G+ C A I A+ + +A Sbjct: 254 AQELGG-KSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELALA 312 Query: 300 ALAERAGKLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFE 359 LA+ +K+ + A GP+ A R++ +I G+++GA L+ G G + G Sbjct: 313 TLAQ----MKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPG-RPDGLA 367 Query: 360 KGFFVGGTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDG 419 +G++ T+ V +M + +EEIFGPVL +L + A+ + N YG G V RD Sbjct: 368 QGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDK 427 Query: 420 GVAREFVRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFGDHHMYGPEGVRFYTRAKAVM 478 R +I+ G V +N P P A FGG+K+S G+ YG EG+ Y KA++ Sbjct: 428 DQVRAVAARIQSGQVHLNYPAWDPQA--PFGGYKQS--GNGREYGVEGMEEYMEVKAIL 482 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 207 bits (527), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 138/453 (30%), Positives = 222/453 (49%), Gaps = 6/453 (1%) Query: 7 YIGGQRVDTKSGRYADVFNPAQGVPVAQVALGTSDEVDAAVRAGVAAFPAWSNTPPLTRA 66 ++ GQ D G V NPA G + V L + E A+ A AA+PAW R+ Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 67 RVLFRFLHLIQQRADDIARIIVREHGKTFSDAQGEVARGIEIVEFAAGIPQMLKGEYTDQ 126 +L R+ L+ DD+A I+ E GK ++A+GE+ +E+ A + G+ Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPS 134 Query: 127 IARGIDAWSMRQALGVVAGITPFNFPAMVPMWMFPIAIACGNCFVLKPSERDPSASLLIA 186 + +++A+GV A ITP+NFPA + A+A G VLKP+E P ++L +A Sbjct: 135 PSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSALALA 194 Query: 187 DLLKEAGLPDGVFNVLQGDKVTVDA-LLDHPQVKAISFVGSTPIAEYIYARGSAKGKRVQ 245 L + AG+P GVF+V+ G + + +P V+ ISF GST + + + + ++ K++ Sbjct: 195 VLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLS 254 Query: 246 ALGGAKNHMVVMPDADMQLAVDALMGAAYGSAGERCMAISVAVAVGSAGDELVAALAERA 305 G +V DAD+ AV+ + + Y +AG+ C+ + D A L E Sbjct: 255 LELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAV 314 Query: 306 GKLKINEGMAAGAEMGPVVTAAAKERIEGLIGRGVEEGATLVVDGRGYQVPGFEKGFFVG 365 K K+ +G G GP++ A +++E + V +GA +++ G+ + + F Sbjct: 315 KKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHAL----GHSFFE 370 Query: 366 GTLLDHVTPEMTVYKEEIFGPVLCVLRCPDIASAVELINAHEYGNGVAVYTRDGGVAREF 425 T+L VTP M V +EE FGP+ + R + L N E+G Y+RD G Sbjct: 371 PTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRV 430 Query: 426 VRQIEVGMVGVNVPLPVPMAFSSFGGWKRSLFG 458 +E GMVG+N L + + FGG K+S G Sbjct: 431 AEGLESGMVGINTGL-ISNEVAPFGGVKQSGLG 462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,586,285 Number of extensions: 69942 Number of successful extensions: 329 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 280 Number of HSP's successfully gapped: 20 Length of query: 497 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 408 Effective length of database: 1,198,455 Effective search space: 488969640 Effective search space used: 488969640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)