BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0365.p (167 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 335 1e-93 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 89 3e-19 gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-ox... 78 3e-16 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 73 1e-14 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 67 7e-13 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 335 bits (859), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 167/167 (100%), Positives = 167/167 (100%) Query: 1 VEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNGPDGIELFHGYTYSGHPLACAAGLAS 60 VEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNGPDGIELFHGYTYSGHPLACAAGLAS Sbjct: 276 VEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNGPDGIELFHGYTYSGHPLACAAGLAS 335 Query: 61 LEVFQTEGVLENAQAVADYFQEAAHALRGLPHVIDVRTIGLIAGIELASIPGRVGARAYD 120 LEVFQTEGVLENAQAVADYFQEAAHALRGLPHVIDVRTIGLIAGIELASIPGRVGARAYD Sbjct: 336 LEVFQTEGVLENAQAVADYFQEAAHALRGLPHVIDVRTIGLIAGIELASIPGRVGARAYD 395 Query: 121 AFVRAFNDGILIRVTGDIIALSPPLIINKQQIDELFGKLAAILKTLA 167 AFVRAFNDGILIRVTGDIIALSPPLIINKQQIDELFGKLAAILKTLA Sbjct: 396 AFVRAFNDGILIRVTGDIIALSPPLIINKQQIDELFGKLAAILKTLA 442 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 88.6 bits (218), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 57/171 (33%), Positives = 89/171 (52%), Gaps = 8/171 (4%) Query: 1 VEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNG-PDGIELFHGYTYSGHPLACAAGLA 59 V+PD+ T AKGLT G PMGAV + +++ +G P + HG TYS HP++ A L Sbjct: 274 VQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMSTVVGHGQTYSAHPVSAAVALE 333 Query: 60 SLEVFQTEGVLENAQAVADYFQEAAHALRGLPHVIDVRTIGLIAGIELAS---IPGRVGA 116 L +++ G+L+N + + F AL P V D R+ GL+ +EL + R Sbjct: 334 VLRLYEEGGLLDNGRRLEPVFAGGLRALLAHPLVGDARSRGLLGALELVADKQTKARFAP 393 Query: 117 --RAYDA-FVRAFNDGILIRVTGD-IIALSPPLIINKQQIDELFGKLAAIL 163 + +D F A+ G++ R GD I+ +P L ++ +F +L AIL Sbjct: 394 ELKLHDRIFQAAYRHGLVFRAFGDNILGFAPALCYTQEDFALMFARLKAIL 444 >gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 78.2 bits (191), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 54/166 (32%), Positives = 82/166 (49%), Gaps = 8/166 (4%) Query: 1 VEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNGPDGIELFHGYTYSGHPLACAAGLAS 60 V PD+ +KG++ G +P+ V I+ AF + H ++Y+G+PLAC A LA+ Sbjct: 277 VWPDLLCLSKGISGGYLPLSLVMSSEAIYQAFYDDDVRRGFLHSHSYTGNPLACRAALAT 336 Query: 61 LEVFQTEGVLENAQAVADYFQEAAHALRGLPHVIDVRTIGLIAGIELASIPGRVGARAYD 120 L +F + VLE +A A A L P V VR G+I + A + G A A Sbjct: 337 LAIFAEDAVLERNRAFAPVLAAALQPLAQDPRVRHVRQRGMIWAYD-AVLEG--SAAATT 393 Query: 121 AFVR-----AFNDGILIRVTGDIIALSPPLIINKQQIDELFGKLAA 161 AF R A +L+R G + L PP +++++Q L +L A Sbjct: 394 AFSRRMAAEAAQREVLVRPIGASLYLMPPYLLDEEQAHWLGRQLHA 439 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 72.8 bits (177), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 58/170 (34%), Positives = 86/170 (50%), Gaps = 13/170 (7%) Query: 1 VEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNGPDGIELFHGYTYSGHPLACAAGLAS 60 V PD+ T AK L G+ P+ AV + EI DA P G+ G TY+G+PLA A+ LA Sbjct: 262 VLPDLMTMAKSLAGGM-PLSAVNGRAEIMDA--PAPGGL----GGTYAGNPLAIASALAV 314 Query: 61 LEVFQTEGVLENAQAVADYFQEAAHALR-GLPHVIDVRTIGLIAGIELASIPGRVGARAY 119 L+V + E ++ Q + D QE LR +P + +VR +G + +E A Y Sbjct: 315 LDVMEEEQLVTRGQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVAVEFADPATGKPDAEY 374 Query: 120 DAFVR--AFNDGILIRVT---GDIIALSPPLIINKQQIDELFGKLAAILK 164 V+ A N+G+L+ G++I PL I +DE G LA ++ Sbjct: 375 TKKVQQHALNNGLLLLTCGSYGNVIRFLFPLTIPDTVMDEALGILAKAIR 424 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 67.0 bits (162), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 60/175 (34%), Positives = 83/175 (47%), Gaps = 17/175 (9%) Query: 1 VEPDIFTTAKGLTNGVVPMGAVFVKPEIHDAFMNGPDGIELFHGYTYSGHPLACAAGLAS 60 V PDI AKGL G P+GA+ I+DA + G + HG+TY GH ACAA +A Sbjct: 260 VVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQ--HGHTYIGHATACAAAVAV 317 Query: 61 LEVFQTEGVLENAQAVADYFQ-EAAHALRGLPHVIDVRTIGLIAGIEL-----ASIP--- 111 + Q E +LEN + + + E A HV D+R GL G+EL + +P Sbjct: 318 QKTIQEERLLENVRQRGEQLRSELRQAFGDQAHVGDIRGRGLFVGVELVAERSSKLPLSP 377 Query: 112 -----GRVGARAYDAFVRAFNDGILIR-VTGDIIALSPPLIINKQQIDELFGKLA 160 RV A A + + G I GD I L+PP I + I E+ +L+ Sbjct: 378 DLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILLAPPFIASSNDISEIVQRLS 432 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.142 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 559,894 Number of extensions: 24016 Number of successful extensions: 86 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 80 Number of HSP's successfully gapped: 8 Length of query: 167 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 88 Effective length of database: 1,245,805 Effective search space: 109630840 Effective search space used: 109630840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)