BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0364.p (255 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate amino... 462 e-131 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 101 6e-23 gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-ox... 93 2e-20 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 76 2e-15 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 60 2e-10 >gi|300313929|ref|YP_003778021.1| omega-amino acid/pyruvate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 442 Score = 462 bits (1188), Expect = e-131, Method: Compositional matrix adjust. Identities = 224/224 (100%), Positives = 224/224 (100%) Query: 1 MSSQDPTHPSSLSAYWMPFTANRNFKAAPRMLVSAEGMHYRDDAGNTILDGTAGLWCVPL 60 MSSQDPTHPSSLSAYWMPFTANRNFKAAPRMLVSAEGMHYRDDAGNTILDGTAGLWCVPL Sbjct: 1 MSSQDPTHPSSLSAYWMPFTANRNFKAAPRMLVSAEGMHYRDDAGNTILDGTAGLWCVPL 60 Query: 61 GHAHPKIVSAVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVD 120 GHAHPKIVSAVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVD Sbjct: 61 GHAHPKIVSAVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVD 120 Query: 121 TALKIALAYHRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLP 180 TALKIALAYHRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLP Sbjct: 121 TALKIALAYHRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLP 180 Query: 181 HTHNLEKNAFTKGEPEYGAHLADELERLVTLHDASNIAAVIVEP 224 HTHNLEKNAFTKGEPEYGAHLADELERLVTLHDASNIAAVIVEP Sbjct: 181 HTHNLEKNAFTKGEPEYGAHLADELERLVTLHDASNIAAVIVEP 224 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 101 bits (251), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 64/207 (30%), Positives = 98/207 (47%), Gaps = 1/207 (0%) Query: 17 MPFTANRNFKAAPRMLVSAEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVSAVQKSVA 76 M +RN ++ M V+ EGM D G +D + G LGH HP+++ A++K V Sbjct: 1 MTHVFHRNPRSKLPMAVAGEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVG 60 Query: 77 TLDYAPSFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAYHRAKGNA 136 L YA + A ELA L +V++ GSEAV+ ALK+A Y+ G Sbjct: 61 ELAYAHTSFFTTAPAEELAAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVEVGQP 120 Query: 137 TRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNAFTKGEPE 196 R +I R + YHG G +++GG R+ F L+ H+ + A + + + Sbjct: 121 QRRHIIARRQSYHGNTLGALAIGGNAWRREMFMPMLIEA-HHVSPCYAYRNRADGESDVQ 179 Query: 197 YGAHLADELERLVTLHDASNIAAVIVE 223 Y LADELE+ + A + A + E Sbjct: 180 YVQRLADELEQKILSLGADQVIAFVAE 206 >gi|300311765|ref|YP_003775857.1| adenosylmethionine-8-amino-7-oxononanoate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 448 Score = 92.8 bits (229), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 63/215 (29%), Positives = 101/215 (46%), Gaps = 4/215 (1%) Query: 11 SLSAYWMPFTANRNFKAAPRMLVS-AEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVS 69 SL + W P T R ++AP + ++ A G+ D G LD + W GH + +I + Sbjct: 13 SLRSVWHPCTQMRRHESAPPIAITRAHGLWLHDSDGRRYLDAISSWWVNLFGHTNTRINA 72 Query: 70 AVQKSVATLDYAPSFQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAY 129 A+ + + L++A H L+E+L A TG G+ FY G+ AV+ ALK+++ Sbjct: 73 ALVEQLGQLEHAMLAGFTHEPVVALSEQLAALTGQALGHCFYASDGASAVEIALKMSVHA 132 Query: 130 HRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNA 189 R G + I GYHG G + V R + + L P H+ + + + A Sbjct: 133 WRNGGQPDKNEFICIAGGYHGETVGALGVTDTPLFRSAYESLLRPA--HVAASPDARQAA 190 Query: 190 FTKGEPEYGAHLADELERLVTLHDASNIAAVIVEP 224 + E A D++ER++ IAA+IVEP Sbjct: 191 PGESAEEVAARALDDVERILQ-QRQGRIAALIVEP 224 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 76.3 bits (186), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 58/209 (27%), Positives = 90/209 (43%), Gaps = 5/209 (2%) Query: 18 PFTANRNFKA-APRMLVSAEGMHYRDDAGNTILDGTAGLWCVPLGHAHPKIVSAVQKSVA 76 P T+ R + P +L S +G + D +LD +GLWCV G+ ++ A + + Sbjct: 17 PVTSLREHEELGPLILKSGQGAYLTDHQDKQLLDAFSGLWCVNTGYGQESVIRAATEQMQ 76 Query: 77 TLDYAPS-FQLGHPSAFELAERLKAYTGNRFGNVFYTGSGSEAVDTALKIALAYHRAKGN 135 L YA F A LA++L + +V++T GS+AVD A++ YH + G Sbjct: 77 RLPYATGYFHFASEPAILLAKKLVDISPASLQHVYFTLGGSDAVDAAIRYITHYHHSIGK 136 Query: 136 ATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVDHLPHTHNLEKNAFTKGEP 195 + I +RGYHG G + + F+ LP +LP + +G+ Sbjct: 137 PGKQHFISLQRGYHGSSSVGAGLTALPNFHYHFNLP-LPTQHYLPSPYPYRSE--LQGDE 193 Query: 196 EYGAHLADELERLVTLHDASNIAAVIVEP 224 A L V A N+AA EP Sbjct: 194 AIIAASVQALRDKVAELGADNVAAFFCEP 222 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 64/230 (27%), Positives = 107/230 (46%), Gaps = 22/230 (9%) Query: 6 PTHPSSLSAYWMPFTANRNFKA-----APRMLVSAEGMHYRDDAGNTILDGTAGLWCVPL 60 PT+P +A + +A A P + A G+ ++D GNT +D +AG+ + Sbjct: 20 PTYPGPKTAAALELSARTESMARGGGRMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSV 79 Query: 61 GHAHPKIVSAVQKSVATLDYAPSFQLGHPSAFELAERLKAYT--GNRFGNV-FYTGSGSE 117 G +P++V A++K +L + S ++ ELA ++ G R + F+T GS+ Sbjct: 80 GRCNPRVVEAIRKQSESLMH--SMEVNSSKRTELAAKISEIMPDGLRGDCITFFTQGGSD 137 Query: 118 AVDTALKIALAYHRAKGNATRTRLIGRERGYHGVGFGGMSVGGIGGNRKTFSTALLPGVD 177 A++ A+K A R G R ++I GYHG+ ++ RK F + GV Sbjct: 138 ALEAAVKFA---KRVTG---RHQIIAFHGGYHGIWNASNALTTGTAYRKGFG-PFMGGVI 190 Query: 178 H--LPHTHNLEKNAFTKGEPEYGAHLADELERLVTLHDAS-NIAAVIVEP 224 H P+ + + K + D L L T + A+ ++AAVIVEP Sbjct: 191 HAPYPYAYRFPFDTSHKSAEQIAGEYVDYL--LNTPYTAADDVAAVIVEP 238 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 812,854 Number of extensions: 31700 Number of successful extensions: 94 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 92 Number of HSP's successfully gapped: 8 Length of query: 255 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 172 Effective length of database: 1,226,865 Effective search space: 211020780 Effective search space used: 211020780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)