BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0354.p (522 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313925|ref|YP_003778017.1| methyl-accepting chemotaxis sen... 1017 0.0 gi|300309796|ref|YP_003773888.1| methyl-accepting chemotaxis pro... 397 e-112 gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis tra... 390 e-109 gi|300312783|ref|YP_003776875.1| methyl-accepting chemotaxis tra... 373 e-104 gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis tra... 365 e-102 >gi|300313925|ref|YP_003778017.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 522 Score = 1017 bits (2629), Expect = 0.0, Method: Compositional matrix adjust. Identities = 516/522 (98%), Positives = 520/522 (99%) Query: 1 MDFTRLTVSSRLKFGFGVVLFLILLIVGIALEKLAEVNRQLVSITEVNNVEIYHLSTMRA 60 MDFTRLTVSSRLKFGFGVVLFLILLIVGIAL+KLAEVNRQLVSITEVNNVEIYHLSTMRA Sbjct: 1 MDFTRLTVSSRLKFGFGVVLFLILLIVGIALKKLAEVNRQLVSITEVNNVEIYHLSTMRA 60 Query: 61 AAYEQSLASRGIGLALTPEDLNQNANNLQRQIGLYTQSEAALAKLFADLQETTDTEKQAI 120 AAYEQSLASRGIGLALTPEDLNQNANNLQRQIGLYTQSEAALAKLFADLQETTDTEKQA+ Sbjct: 61 AAYEQSLASRGIGLALTPEDLNQNANNLQRQIGLYTQSEAALAKLFADLQETTDTEKQAL 120 Query: 121 AAIQKMSAGLSPGLSNMIDLAKAQKTEELKPIMTGPLGKMQADRRKALAELSAFEDKLNN 180 AAIQKMSAGLSPGLSNMIDLAKAQKTEELKPIMTGPLGKMQADRRKALAELSAFEDKLNN Sbjct: 121 AAIQKMSAGLSPGLSNMIDLAKAQKTEELKPIMTGPLGKMQADRRKALAELSAFEDKLNN 180 Query: 181 EAKDEALAVSASARKLLIALGVIALLCGLMAAVLITRSVLRQLGGEPAYASRLAAQIAKG 240 EAKDEALAVSASARKLLIALGVIALLCGLMAAVLITRSVLRQLGGEPAYASRLAAQIAKG Sbjct: 181 EAKDEALAVSASARKLLIALGVIALLCGLMAAVLITRSVLRQLGGEPAYASRLAAQIAKG 240 Query: 241 DLRTEVKADTRHPESLMMSMKTMNEGLRGIVREVRSGTDAIATASSQIASGNLDLSSRTE 300 DLRTEVKADTRHPESLMMSMKTMNEGLRGIVREVRSGTDAIATASSQIASGNLDLSSRTE Sbjct: 241 DLRTEVKADTRHPESLMMSMKTMNEGLRGIVREVRSGTDAIATASSQIASGNLDLSSRTE 300 Query: 301 EQASSLEETASAMEQLTATVKQNADSAGEANRLAAEASAVASQSGQLVQEVVTTMGAIED 360 EQASSLEETASAMEQLTATVKQNADSAGEANRLAAEASAVASQSGQLV+EVVTTM AIED Sbjct: 301 EQASSLEETASAMEQLTATVKQNADSAGEANRLAAEASAVASQSGQLVKEVVTTMAAIED 360 Query: 361 SSKKIVDIISVIDGISFQTNILALNAAVEAARAGEQGRGFAVVAAEVRTLAQRSSAAARE 420 SSKKIVDIISVIDGISFQTNILALNAAVEAARAGEQGRGFAVVAAEVRTLAQRSSAAARE Sbjct: 361 SSKKIVDIISVIDGISFQTNILALNAAVEAARAGEQGRGFAVVAAEVRTLAQRSSAAARE 420 Query: 421 IKALIDDSVDKVASGSALVNRAGDTMSDVVSSVDRVVSVIGAISDSSAEQSKGIQEINHA 480 IKALIDDSVDKVASGSALVNRAGDTMSDVVSSVDRVVSVIGAISDSSAEQSKGIQEINHA Sbjct: 421 IKALIDDSVDKVASGSALVNRAGDTMSDVVSSVDRVVSVIGAISDSSAEQSKGIQEINHA 480 Query: 481 ISQMDQVTQQNAALVEEAAAASQALQAQAKRLSDVVEVFAVA 522 ISQMDQVTQQNAALVEEAAAASQALQAQA+RLS VVEVFAVA Sbjct: 481 ISQMDQVTQQNAALVEEAAAASQALQAQARRLSGVVEVFAVA 522 >gi|300309796|ref|YP_003773888.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 397 bits (1021), Expect = e-112, Method: Compositional matrix adjust. Identities = 239/514 (46%), Positives = 341/514 (66%) Query: 6 LTVSSRLKFGFGVVLFLILLIVGIALEKLAEVNRQLVSITEVNNVEIYHLSTMRAAAYEQ 65 + + +RL GF V+ L++L+ G + + +V + + EV N E + M E+ Sbjct: 5 MKMGTRLGLGFAWVVLLLVLVTGFGIFNMHQVQGRFTHVVEVKNPESALVKEMLETVGER 64 Query: 66 SLASRGIGLALTPEDLNQNANNLQRQIGLYTQSEAALAKLFADLQETTDTEKQAIAAIQK 125 S++ R + L PED++ A ++ Q Y + L +LFA+ +T+D EK A+ I Sbjct: 65 SISLRNLMLPAAPEDVDIEAQYIEAQSLKYKMAAQKLQQLFAESSDTSDEEKAALPKIAA 124 Query: 126 MSAGLSPGLSNMIDLAKAQKTEELKPIMTGPLGKMQADRRKALAELSAFEDKLNNEAKDE 185 ++ ++ ++ K ++ EL + +Q + L L+A ED+LN EA + Sbjct: 125 QASKAEKLINEAVEFGKRREAYELAQFLKEQYLPVQKSWQVELKALAALEDQLNKEAAEA 184 Query: 186 ALAVSASARKLLIALGVIALLCGLMAAVLITRSVLRQLGGEPAYASRLAAQIAKGDLRTE 245 + + AR L++ + I ++ ++AA +TR +LR+LGGEP YA ++A QIA+GDL Sbjct: 185 SAVAYSRARMLMLLISAIGVVTAVLAAFWLTRHLLRKLGGEPDYAVQIAGQIAEGDLAVV 244 Query: 246 VKADTRHPESLMMSMKTMNEGLRGIVREVRSGTDAIATASSQIASGNLDLSSRTEEQASS 305 + +SLM +M+ M + L IV EVR G++ I TASS+IA GNLDLS RTE+QA + Sbjct: 245 IDTRAGDHDSLMAAMRVMRDKLAAIVAEVRKGSETITTASSEIARGNLDLSGRTEQQAGA 304 Query: 306 LEETASAMEQLTATVKQNADSAGEANRLAAEASAVASQSGQLVQEVVTTMGAIEDSSKKI 365 LEETAS+MEQL +TVKQNA++A +AN LA AS VA Q G +V +VV TM +I SS+K+ Sbjct: 305 LEETASSMEQLNSTVKQNAENAHQANDLALAASDVAVQGGSIVSQVVQTMQSINASSQKV 364 Query: 366 VDIISVIDGISFQTNILALNAAVEAARAGEQGRGFAVVAAEVRTLAQRSSAAAREIKALI 425 DI+SVIDGI+FQTNILALNAAVEAARAGEQGRGFAVVA+EVR+LAQRS++AA+EIK LI Sbjct: 365 ADIVSVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKVLI 424 Query: 426 DDSVDKVASGSALVNRAGDTMSDVVSSVDRVVSVIGAISDSSAEQSKGIQEINHAISQMD 485 DSV KV +GS LV +AG TM D+V+S+ RV V+ I+ ++ EQS GI+++N AI Q+D Sbjct: 425 GDSVGKVENGSKLVEQAGSTMHDIVTSIRRVTDVMTEINHANREQSSGIEQVNEAIIQID 484 Query: 486 QVTQQNAALVEEAAAASQALQAQAKRLSDVVEVF 519 +TQQNAALVE+AAAA+++LQ QA L +V VF Sbjct: 485 DMTQQNAALVEQAAAAARSLQEQAAGLQQLVSVF 518 >gi|300309873|ref|YP_003773965.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 547 Score = 390 bits (1003), Expect = e-109, Method: Compositional matrix adjust. Identities = 244/516 (47%), Positives = 324/516 (62%) Query: 6 LTVSSRLKFGFGVVLFLILLIVGIALEKLAEVNRQLVSITEVNNVEIYHLSTMRAAAYEQ 65 L V +RL GFG++L +L I + L +A VN L IT+VNN EI ++ +R A ++ Sbjct: 4 LKVVTRLALGFGLILVFLLSISALGLYGMARVNDALTDITDVNNAEIKLVTALRNALSQR 63 Query: 66 SLASRGIGLALTPEDLNQNANNLQRQIGLYTQSEAALAKLFADLQETTDTEKQAIAAIQK 125 +LA R I L D+ + L RQ LY + AAL K+F TT+ E+Q + I+ Sbjct: 64 ALAIRNIALLDEQADMQVESQTLARQEKLYADAYAALDKMFTTEAATTERERQLLVQIKN 123 Query: 126 MSAGLSPGLSNMIDLAKAQKTEELKPIMTGPLGKMQADRRKALAELSAFEDKLNNEAKDE 185 A P ++ + L K E ++ + Q K+L EL+ FED+LN+EA Sbjct: 124 DEAATLPLMAKAVQLGLENKAAEAGKMLITQVRPKQQAWIKSLTELAEFEDQLNDEAATS 183 Query: 186 ALAVSASARKLLIALGVIALLCGLMAAVLITRSVLRQLGGEPAYASRLAAQIAKGDLRTE 245 A A R + +A + ALL G+ A+ LI RS+LRQLG EP+ A +A IA GDL Sbjct: 184 ARNTYAWLRMVTVAAALAALLVGVAASALIARSILRQLGAEPSEAQAVARDIASGDLTAT 243 Query: 246 VKADTRHPESLMMSMKTMNEGLRGIVREVRSGTDAIATASSQIASGNLDLSSRTEEQASS 305 V+ SLM S++ M L I ++S + I+ AS QIA GN DLS RTEEQA+S Sbjct: 244 VRVREGDSSSLMASIEQMRYQLNVIAHGIKSAAETISVASGQIAQGNFDLSQRTEEQAAS 303 Query: 306 LEETASAMEQLTATVKQNADSAGEANRLAAEASAVASQSGQLVQEVVTTMGAIEDSSKKI 365 LEETA++ME+LT TV+QN A EA RL+ A+ A+ Q VV TM I SS K+ Sbjct: 304 LEETAASMEELTTTVRQNTQHASEALRLSGGATETAAAGSQAFDRVVQTMDRITASSSKM 363 Query: 366 VDIISVIDGISFQTNILALNAAVEAARAGEQGRGFAVVAAEVRTLAQRSSAAAREIKALI 425 DIISVI+GI+FQTNILALNAAVEAARAGEQGRGFAVVA+EVR+LAQRS+ AA+EIK LI Sbjct: 364 SDIISVIEGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAVAAKEIKELI 423 Query: 426 DDSVDKVASGSALVNRAGDTMSDVVSSVDRVVSVIGAISDSSAEQSKGIQEINHAISQMD 485 +S+ V +GS LV+ AG M+ +V+SV R ++ I+ ++ EQ GI++IN A+ QMD Sbjct: 424 GESMSHVGAGSQLVSDAGQQMAQIVASVQRFGEIMNNIATATQEQGSGIEQINTAMVQMD 483 Query: 486 QVTQQNAALVEEAAAASQALQAQAKRLSDVVEVFAV 521 QVTQQNAALVEEA+AA+Q+L QA L VV VF + Sbjct: 484 QVTQQNAALVEEASAAAQSLSQQASGLLKVVSVFKI 519 >gi|300312783|ref|YP_003776875.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 373 bits (957), Expect = e-104, Method: Compositional matrix adjust. Identities = 236/520 (45%), Positives = 335/520 (64%), Gaps = 14/520 (2%) Query: 3 FTRLTVSSRLKFGFGVVLFLILLIVGIALEKLAEVNRQLVSITEVNNVEIYHLSTMRAAA 62 F + + ++L GFG ++ L +L+ GI+L + + + ++ V + ++ L R A Sbjct: 2 FKHMKIGTKLALGFGALVVLTILVSGISLSSIRALTSSIKAVLAVQDQKLAPLYAAREAL 61 Query: 63 YEQSLASRGIGLALTPEDLNQNANNLQRQIGLYTQSEAALAKLFADLQETTDTEKQAIAA 122 + +A+R + + D + + Q LY ++ L LFA +E EK Sbjct: 62 DQTGIAARNAYVFRSDADAQKELAIVDEQKQLYLKAIKDLGPLFAGNKEF---EKVRSGM 118 Query: 123 IQKMSAGLSPGLSNMIDLAKAQKTEELKPIMTGPLGKMQADRRKALAELSAFEDKL---N 179 +Q P L ++ ++EE + + + RR+ ++++ ++ N Sbjct: 119 LQMAEELKRPRLYR-----ESGRSEEYRDFLVNECSPL---RRQIVSDIGTVLAQVEAEN 170 Query: 180 NEAKDEALAVSASARKLLIALGVIALLCGLMAAVLITRSVLRQLGGEPAYASRLAAQIAK 239 N A+D ++ A + L ++ ++ ++ AVL+TR +L+QLGGEP YA+++A QIA Sbjct: 171 NRAQDATDKMAQLAALTVAVLTLVVVVISILIAVLLTRGLLKQLGGEPTYAAQIAQQIAD 230 Query: 240 GDLRTEVKADTRHPESLMMSMKTMNEGLRGIVREVRSGTDAIATASSQIASGNLDLSSRT 299 GDL V+ T SL+ ++K M + L IV +VR GT I ASS+IASGNLDLSSRT Sbjct: 231 GDLTANVQTKTGDNSSLLFAIKNMRDSLVSIVAQVRQGTFTIEQASSEIASGNLDLSSRT 290 Query: 300 EEQASSLEETASAMEQLTATVKQNADSAGEANRLAAEASAVASQSGQLVQEVVTTMGAIE 359 E+QASSLEETASAME+L TVKQNAD+A +A++LA AS VASQ G++V +VVTTM I Sbjct: 291 EQQASSLEETASAMEELMTTVKQNADNAKQASQLAVSASEVASQGGEVVAQVVTTMDGIN 350 Query: 360 DSSKKIVDIISVIDGISFQTNILALNAAVEAARAGEQGRGFAVVAAEVRTLAQRSSAAAR 419 SS+KIVDIISVIDGI+FQTNILALNAAVEAARAGEQGRGFAVVA+EVR+LAQRS+ AA+ Sbjct: 351 ASSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSATAAK 410 Query: 420 EIKALIDDSVDKVASGSALVNRAGDTMSDVVSSVDRVVSVIGAISDSSAEQSKGIQEINH 479 EI LI+DSV ++ GS+LV +AG TM +VV+SV RV V+ IS +S EQS GI ++N Sbjct: 411 EITELINDSVSQIERGSSLVEQAGQTMENVVNSVKRVTDVVAEISMASQEQSHGITQVNT 470 Query: 480 AISQMDQVTQQNAALVEEAAAASQALQAQAKRLSDVVEVF 519 AI+ MD+ TQQN+ALVEEAAAA+ +++ QA LS +V F Sbjct: 471 AITHMDEATQQNSALVEEAAAAANSMKEQAGALSGLVATF 510 >gi|300310789|ref|YP_003774881.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 365 bits (937), Expect = e-102, Method: Compositional matrix adjust. Identities = 235/522 (45%), Positives = 330/522 (63%), Gaps = 8/522 (1%) Query: 3 FTRLTVSSRLKFGFGVVLFLILLIVGIALEKLAEVNRQLVSITEVNNVEIYHLSTMRAAA 62 +L VS+RL GF V+ L+++I + L +A V L I NN + M + Sbjct: 1 MNKLKVSTRLALGFASVVVLLVVISLVGLWGMATVKGNLSDIV-ANNQQGGLADVMNNSV 59 Query: 63 YEQSLASRGIGLALTPEDLNQNANNLQRQIGLYTQSEAALAKLFADLQETTDTEKQAIAA 122 ++LA R I L P + + ++ Q Y + L K+F D +TT E + Sbjct: 60 RIRALAIRNIALFQEPALIAREIERIKVQEKAYQDAREKLGKMF-DQPDTTRREHELFDL 118 Query: 123 IQKMSAGLSPGLSNMIDLAKAQKTEELKPIMTGPLGKMQADRRKALAELSAFEDKLNNEA 182 I + +A P ++ + LA+ K+ EL ++T + +QA L EL FED+LNN+A Sbjct: 119 IAQNAADTEPLMNQAVTLAQQDKSAELISLLTQKIRPVQAKWLDNLDELGRFEDELNNQA 178 Query: 183 KDEALAVSASARKLLIALGVI---ALLCGLMAAVLITRSVLRQLGGEPAYASRLAAQIAK 239 + A + R +L + ++L + AA LI RSVL QLGGEPA A LAAQIA+ Sbjct: 179 ---SAAADTTYRSILTTTWTLVAGSVLLAVAAAFLIVRSVLHQLGGEPAQAQELAAQIAQ 235 Query: 240 GDLRTEVKADTRHPESLMMSMKTMNEGLRGIVREVRSGTDAIATASSQIASGNLDLSSRT 299 G+L + SLM S++ M L IV ++ ++I+TA+SQIA GNLDLS RT Sbjct: 236 GNLAVSIPLAKGDQHSLMASLENMRAQLTRIVDGIKHSAESISTAASQIAVGNLDLSRRT 295 Query: 300 EEQASSLEETASAMEQLTATVKQNADSAGEANRLAAEASAVASQSGQLVQEVVTTMGAIE 359 EEQA+SLEETAS+ME++T+TV+QN +AG+ ++LA AS A + G++V+ VV TM I Sbjct: 296 EEQAASLEETASSMEEITSTVEQNTQNAGQGHQLARSASLHAQKGGEVVERVVGTMKDIS 355 Query: 360 DSSKKIVDIISVIDGISFQTNILALNAAVEAARAGEQGRGFAVVAAEVRTLAQRSSAAAR 419 S+ KI +II+ ++GI+FQTNILALNAAVEAARAGEQGRGFAVVA EVR+LAQRS+++A+ Sbjct: 356 QSAYKITEIIATVEGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASSAK 415 Query: 420 EIKALIDDSVDKVASGSALVNRAGDTMSDVVSSVDRVVSVIGAISDSSAEQSKGIQEINH 479 EI+ LI+ SV V +G LV+ AG TM +VV S+ +V ++ I+ +S EQS GI +IN Sbjct: 416 EIRGLIESSVQHVEAGQKLVDEAGLTMDEVVRSIQQVNDLMSEITAASNEQSSGISQINI 475 Query: 480 AISQMDQVTQQNAALVEEAAAASQALQAQAKRLSDVVEVFAV 521 AISQMD+VTQQNAALVEE+A+A+Q++ QA L D V VF + Sbjct: 476 AISQMDEVTQQNAALVEESASAAQSMAQQANSLRDAVAVFRI 517 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.124 0.312 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,030,392 Number of extensions: 34588 Number of successful extensions: 500 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 298 Number of HSP's successfully gapped: 71 Length of query: 522 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 432 Effective length of database: 1,193,720 Effective search space: 515687040 Effective search space used: 515687040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)