BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0351.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313922|ref|YP_003778014.1| LysR family transcription regul... 626 0.0 gi|300309921|ref|YP_003774013.1| LysR family transcription regul... 204 1e-53 gi|300311458|ref|YP_003775550.1| LysR family transcription regul... 199 2e-52 gi|300312196|ref|YP_003776288.1| LysR family transcription regul... 186 2e-48 gi|300309660|ref|YP_003773752.1| LysR family transcription regul... 178 5e-46 >gi|300313922|ref|YP_003778014.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 626 bits (1615), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/308 (100%), Positives = 308/308 (100%) Query: 1 MDRLMAMQVFVTVVDSGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 MDRLMAMQVFVTVVDSGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP Sbjct: 1 MDRLMAMQVFVTVVDSGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 Query: 61 AGNELLPRCRQMLEYSEDMRHALDAPDETPRGLLRITASTSFAQTQLIQAVVEYGRRYPG 120 AGNELLPRCRQMLEYSEDMRHALDAPDETPRGLLRITASTSFAQTQLIQAVVEYGRRYPG Sbjct: 61 AGNELLPRCRQMLEYSEDMRHALDAPDETPRGLLRITASTSFAQTQLIQAVVEYGRRYPG 120 Query: 121 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCRSIICATPDYLARHGLPRK 180 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCRSIICATPDYLARHGLPRK Sbjct: 121 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCRSIICATPDYLARHGLPRK 180 Query: 181 VEDLALHNCLTHSYVGRSLWQFDPKKKKARSGRGASAAKPQSVAVGGSISANEVTVLLQA 240 VEDLALHNCLTHSYVGRSLWQFDPKKKKARSGRGASAAKPQSVAVGGSISANEVTVLLQA Sbjct: 181 VEDLALHNCLTHSYVGRSLWQFDPKKKKARSGRGASAAKPQSVAVGGSISANEVTVLLQA 240 Query: 241 VLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGMYGVYASRKHMPATLRTMLDFLA 300 VLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGMYGVYASRKHMPATLRTMLDFLA Sbjct: 241 VLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGMYGVYASRKHMPATLRTMLDFLA 300 Query: 301 QRFAGQPF 308 QRFAGQPF Sbjct: 301 QRFAGQPF 308 >gi|300309921|ref|YP_003774013.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 299 Score = 204 bits (518), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 116/305 (38%), Positives = 167/305 (54%), Gaps = 13/305 (4%) Query: 1 MDRLMAMQVFVTVVDSGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 M++L ++ F+ V D GS AAE L +S+ VSR V ELE +G+RL+ RTTRRLSLT Sbjct: 1 MNKLREIECFIAVADLGSFVKAAEALGISKAAVSRTVLELEARLGSRLMQRTTRRLSLTE 60 Query: 61 AGNELLPRCRQMLEYSEDMRHALDAPDETPRGLLRITASTSFAQTQLIQAVVEYGRRYPG 120 AG L RC+Q++ E+ L + P GLLRI A +F L + R+P Sbjct: 61 AGALYLERCKQIVAALEEADDLLSSGSAKPTGLLRINAPLTFGVLHLAPLWPMFLERHPE 120 Query: 121 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCRSIICATPDYLARHGLPRK 180 +++D+ DR V++++E D+AIR+ + L+ R L +CA+P YL HG P Sbjct: 121 ISLDITLSDRIVDIIDEGYDMAIRIARLSDSTLVHRKLTSTSLHVCASPAYLDAHGTPAH 180 Query: 181 VEDLALHNCLTHSY-VGRSLWQFDPKKKKARSGRGASAAKPQSVAVGGSISANEVTVLLQ 239 +LA H +++SY + WQF+ + P SV V + N +Q Sbjct: 181 PHELAQHQTISYSYNQSKDEWQFNGPE------------GPVSVRVSSRLHVNNGDSCVQ 228 Query: 240 AVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGMYGVYASRKHMPATLRTMLDFL 299 A L G+GI P + + + SGQLV +L DY +LG+Y VY SR H+PA +R MLDFL Sbjct: 229 AALGGIGITRQPTFMIDQYLRSGQLVSILGDYGAPELGIYAVYPSRAHLPAKMRAMLDFL 288 Query: 300 AQRFA 304 + FA Sbjct: 289 TEAFA 293 >gi|300311458|ref|YP_003775550.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 303 Score = 199 bits (506), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 115/306 (37%), Positives = 177/306 (57%), Gaps = 16/306 (5%) Query: 1 MDRLMAMQVFVTVVDSGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 MDRL +M+VF VV+ GS AAE LD+S V +R++A+LE +G RLL+R+TRRLSLT Sbjct: 1 MDRLQSMRVFAKVVEQGSFVRAAELLDISNAVATRFIADLEAHLGTRLLNRSTRRLSLTE 60 Query: 61 AGNELLPRCRQMLEYSEDMRHALDAPDETPRGLLRITASTSFAQTQLIQAVVEYGRRYPG 120 AG L R R +L +D + + P G L I + F Q+QL + + Y + YP Sbjct: 61 AGQAYLERVRMILAEVDDAEALVSLETKRPAGTLAIYCNAGFGQSQLGEMLSAYSQAYPE 120 Query: 121 VAVDLLALDRTVNLVEERIDLAIRMT-NRLEPNLIARPLGVCRSIICATPDYLARHGLPR 179 VA+D+ +R+++LVEE ID+ + + + ++I R LGV R I+C +P Y+AR G P Sbjct: 121 VALDISLSERSIDLVEEGIDIGFFSSMQKFDASMIVRQLGVARVILCTSPAYIARQGAPE 180 Query: 180 KVEDLALHNCL--THSYVGRSLWQFDPKKKKARSGRGASAAKPQSVAVGGSISANEVTVL 237 + EDL+ HNCL +H Y+ R W + + V + + +N +L Sbjct: 181 QPEDLSRHNCLNFSHEYL-RHHWHVN------------TNGGVLDVPIVSKVVSNSGVLL 227 Query: 238 LQAVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGMYGVYASRKHMPATLRTMLD 297 LAG+GI P+Y++ + SG+LVQ+LP ++ Q+ M VY SR+ + A +R+ + Sbjct: 228 RDLALAGMGIVLRPSYSLGDDLRSGRLVQVLPGFDMGQVIMSMVYPSRRLLSAKVRSFAE 287 Query: 298 FLAQRF 303 F++ RF Sbjct: 288 FISARF 293 >gi|300312196|ref|YP_003776288.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 308 Score = 186 bits (473), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 112/310 (36%), Positives = 169/310 (54%), Gaps = 13/310 (4%) Query: 1 MDRLMAMQVFVTVVDSGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 MD+L AMQ FV VV+SGS AA + S+P VSR V+ELE ++G RLLHRTTRRLSLT Sbjct: 1 MDKLQAMQTFVAVVESGSFVAAVDVTGQSKPAVSRQVSELEQFLGIRLLHRTTRRLSLTD 60 Query: 61 AGNELLPRCRQMLEYSEDMRHALDAPDETPRGLLRITASTSFAQTQLIQAVVEYGRRYPG 120 G RC+++LE +D+ + + G LRI A F QL ++ ++P Sbjct: 61 EGRVYFARCKELLETIQDVESEVGLNSDQACGRLRIGAPQDFGVEQLAPLWGKFTEQHPL 120 Query: 121 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCRSIICATPDYLARHGLPRK 180 + +++ DRT++L+EE D+AIR++ + ++ARPL R I CA P++LARHG P Sbjct: 121 ITLNITLSDRTIDLLEEGFDMAIRISALGDSRMVARPLAPVRMIACAAPEFLARHGTPGT 180 Query: 181 VEDLALHNCLTHSY-VGRSLWQFDPKKKKARSGRGASAAKPQSVAVGGSISANEVTVLLQ 239 +L + +++SY G W F R G + V V ++ N Sbjct: 181 PAELRDYPFISYSYRAGGDDWSF-------RHVHGDTC----QVRVRSTVYVNNGATCRA 229 Query: 240 AVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEP-LQLGMYGVYASRKHMPATLRTMLDF 298 +AG GI P + V + +GQLV+L + QL ++ +Y +R H+P ++ M DF Sbjct: 230 MAIAGQGIAIQPDFIVNAAIQAGQLVELFEGWSTGEQLVVHTLYPTRTHLPPKVQRMRDF 289 Query: 299 LAQRFAGQPF 308 L ++F P+ Sbjct: 290 LIEQFRVPPW 299 >gi|300309660|ref|YP_003773752.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 337 Score = 178 bits (451), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 101/311 (32%), Positives = 163/311 (52%), Gaps = 15/311 (4%) Query: 1 MDRLMAMQVFVTVVDSGSLSAAAEQLDLSRPVVSRYVAELEDWVGARLLHRTTRRLSLTP 60 MDR AMQVF VVD+ S + AA+ L+L R V+ V LE + RLL+RTTRR+SLTP Sbjct: 1 MDRFQAMQVFTAVVDANSFTRAADNLNLPRTTVTTLVQGLESMLQVRLLNRTTRRISLTP 60 Query: 61 AGNELLPRCRQMLEYSEDMRHALDAPDETPRGLLRITASTSFAQTQLIQAVVEYGRRYPG 120 G RC ++L ++ + P+G LRI S + L+ + ++ RYP Sbjct: 61 DGAAYYERCVRILADLDETEQSFRNVTRGPQGRLRIDVPASVGRLMLLPNLCDFYTRYPD 120 Query: 121 VAVDLLALDRTVNLVEERIDLAIRMTNRLEPNLIARPLGVCRSIICATPDYLARHGLPRK 180 + + + DR V+LV+E +D IR+ + +++AR +G +++ CA P YL RHG+P Sbjct: 121 IELMMGMGDRPVDLVQEAVDCVIRIGELQDSSMVARRIGTFQTVTCAAPAYLERHGVPHT 180 Query: 181 VEDLALHNCLTH--SYVGRSL-WQFDPKKKKARSGRGASAAKPQSVAVGGSISANEVTVL 237 +EDL H + + S GR++ W F + V + G ++ NE Sbjct: 181 IEDLQQHKAVHYFSSRTGRTIDWDF------------VVEGVTREVKMNGKLAVNEADAY 228 Query: 238 LQAVLAGVGIGHMPAYTVAPLVASGQLVQLLPDYEPLQLGMYGVYASRKHMPATLRTMLD 297 ++ L G G+ P Y V + G+LV++L ++P + + VY +H+ +R +D Sbjct: 229 VECALQGFGMVQAPLYMVTRHIDEGRLVEVLSQWKPAPMPISVVYLHNRHLSPKVRAFVD 288 Query: 298 FLAQRFAGQPF 308 ++A+ FA P Sbjct: 289 WVAELFATCPL 299 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 939,787 Number of extensions: 38615 Number of successful extensions: 308 Number of sequences better than 1.0e-08: 64 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 64 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)