BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0345.p (491 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspi... 968 0.0 gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspi... 615 e-177 gi|300313782|ref|YP_003777874.1| transmembrane protein [Herbaspi... 427 e-121 gi|300310865|ref|YP_003774957.1| response regulator containing a... 219 4e-58 gi|300312843|ref|YP_003776935.1| response regulator containing a... 204 2e-53 >gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 968 bits (2503), Expect = 0.0, Method: Compositional matrix adjust. Identities = 477/488 (97%), Positives = 481/488 (98%) Query: 1 LKNYLVVILAIAVLMVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNREMLSLS 60 LKNYLVVILAIAVLMVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRN EMLSLS Sbjct: 25 LKNYLVVILAIAVLMVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNLEMLSLS 84 Query: 61 LDTLAYRFQHPLSARRLNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQVSSRLGRGKGA 120 LDTLAYRFQHPLSARRLNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQVSSRLGRG GA Sbjct: 85 LDTLAYRFQHPLSARRLNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQVSSRLGRGNGA 144 Query: 121 PHYADRAYFIAHRDSPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVVMALDL 180 PHYADRAYFIAHRDSPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVVMALDL Sbjct: 145 PHYADRAYFIAHRDSPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVVMALDL 204 Query: 181 AYFRELFEGLALGDEGIISLYSDEGIAYMRVPYRETIIGRDVSQTGNFQRLRAMLSEEEG 240 AYFRELFEGLALGDEGIISLYSDEGIAYMRVPYRETIIGRD+SQTGNFQRLRAMLSEEEG Sbjct: 205 AYFRELFEGLALGDEGIISLYSDEGIAYMRVPYRETIIGRDISQTGNFQRLRAMLSEEEG 264 Query: 241 SFFAHATNDGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAIFGLLTVW 300 SFFAHATNDGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAIFGLLT W Sbjct: 265 SFFAHATNDGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAIFGLLTAW 324 Query: 301 LLLHVRQELRRRVTAERQLEALARTDGLTGLLNRRALDEALQATWERCQRNLGASFSVLF 360 LLLHVRQELRRRVTAERQLEALARTDGLT LLNRRALDEALQATWERCQRNLGASFSVLF Sbjct: 325 LLLHVRQELRRRVTAERQLEALARTDGLTDLLNRRALDEALQATWERCQRNLGASFSVLF 384 Query: 361 IDVDYFKAYNDTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEEFVVLLEQTDLQGA 420 IDVDYFKAYNDTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEEFVVLLEQTDLQGA Sbjct: 385 IDVDYFKAYNDTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEEFVVLLEQTDLQGA 444 Query: 421 QMMAQRLCDAVRERHIAHTSSPLAVVTISVGVATLERDHHRRVEDVLNAADAALYRAKRE 480 QMMAQRLCDAVRERHIAHTSSPLAVVTISVGVATLERDHHRRVEDVLNAADAALYRAK+ Sbjct: 445 QMMAQRLCDAVRERHIAHTSSPLAVVTISVGVATLERDHHRRVEDVLNAADAALYRAKQA 504 Query: 481 GRNAVRVS 488 GR+ V +S Sbjct: 505 GRDRVHLS 512 >gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 615 bits (1585), Expect = e-177, Method: Compositional matrix adjust. Identities = 301/489 (61%), Positives = 378/489 (77%), Gaps = 1/489 (0%) Query: 1 LKNYLVVILAIAVLMVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNREMLSLS 60 L+ YL +IL + ++MV++I ++LY+ Y+QTR SA++ A+NVTL+ QRAISRN E+LSL+ Sbjct: 4 LQKYLALILGLIIMMVLSICGASLYEGYRQTRISAITEANNVTLIVQRAISRNMEILSLA 63 Query: 61 LDTLAYRFQHPLSARRLNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQVSSRLGRGKGA 120 +DTLAYR+QHPL +D +RY YLFGST HI +A+LDADG V+V+SR Sbjct: 64 MDTLAYRYQHPLDLTHASDEERYNYLFGSTAAAHHITALAILDADGYVRVTSRRSASPSV 123 Query: 121 P-HYADRAYFIAHRDSPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVVMALD 179 +Y++R YF HRDS GL++SRP++A+LGNEL VVVLSKRLS +DGSF GVV+MALD Sbjct: 124 VVNYSERTYFTVHRDSADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGVVLMALD 183 Query: 180 LAYFRELFEGLALGDEGIISLYSDEGIAYMRVPYRETIIGRDVSQTGNFQRLRAMLSEEE 239 ++YFR+LF GL LG++GI+SLYSD+G+ YMRVPY + IGR+++ NFQRLRAML + + Sbjct: 184 MSYFRDLFTGLTLGEDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPNFQRLRAMLVDND 243 Query: 240 GSFFAHATNDGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAIFGLLTV 299 G+FFA A DGV RLYTFRRIP +PLIV +G +E IFKTW T Y+VL+ L IF L Sbjct: 244 GNFFARAHTDGVERLYTFRRIPDAPLIVLLGRSERFIFKTWYDTLYSVLLLLIIFTALAG 303 Query: 300 WLLLHVRQELRRRVTAERQLEALARTDGLTGLLNRRALDEALQATWERCQRNLGASFSVL 359 LL+ VR+EL +RV AE+QLE LARTDGLTGLLNRR+LDEAL A W+R QR+ A FS L Sbjct: 304 VLLMLVRRELLKRVAAEKQLEDLARTDGLTGLLNRRSLDEALLAVWQRSQRSPTAGFSTL 363 Query: 360 FIDVDYFKAYNDTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEEFVVLLEQTDLQG 419 FIDVDYFK YNDTYGH++GDTVL+ VAA L L R D AGRYGGEEFVVLLEQTD G Sbjct: 364 FIDVDYFKRYNDTYGHEMGDTVLQAVAAVLREHLQRQNDLAGRYGGEEFVVLLEQTDEHG 423 Query: 420 AQMMAQRLCDAVRERHIAHTSSPLAVVTISVGVATLERDHHRRVEDVLNAADAALYRAKR 479 A MAQ+LC+A+ R I H+ SPL V+++S+GVAT+ R H+R+EDVLNAAD ALY AKR Sbjct: 424 ALRMAQQLCEAIYNRGIPHSGSPLGVISVSIGVATMNRAPHQRMEDVLNAADHALYIAKR 483 Query: 480 EGRNAVRVS 488 EGRN V V+ Sbjct: 484 EGRNRVCVA 492 >gi|300313782|ref|YP_003777874.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 475 Score = 427 bits (1099), Expect = e-121, Method: Compositional matrix adjust. Identities = 217/473 (45%), Positives = 311/473 (65%), Gaps = 6/473 (1%) Query: 15 MVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNREMLSLSLDTLAYRFQHPLSA 74 M +S + LYQS++Q ++A+ + +N+ ++QR+ISRN E+L+L+LD+L +R++H Sbjct: 1 MTATVSCAVLYQSHRQVERAAIEHGANIARIAQRSISRNVELLTLALDSLTWRYRHT-QL 59 Query: 75 RRLNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQV-SSRLGRGKGAPHYADRAYFIAHR 133 + Q+ +L G ++A + ++D DG + V S+ L + P YA +F AH+ Sbjct: 60 HEMPLQQKMEFLLGENIEARYVAALGIVDTDGELVVGSAALSQILPGP-YAQSDFFTAHQ 118 Query: 134 D---SPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVVMALDLAYFRELFEGL 190 GLY+S P+ L +QVVVLSKRL+ DGSFAG+ +M L L YFR+LF+GL Sbjct: 119 THHAQGDTGLYISAPLTVRLERRMQVVVLSKRLAQPDGSFAGISLMVLYLDYFRDLFQGL 178 Query: 191 ALGDEGIISLYSDEGIAYMRVPYRETIIGRDVSQTGNFQRLRAMLSEEEGSFFAHATNDG 250 L ++G+ISLYS +G+AYMR+PY E IG ++ + + +R+ + +GS+FA A DG Sbjct: 179 TLENDGVISLYSADGLAYMRIPYNEAFIGSRLNISEDLERIFSPSGPAQGSYFARAQQDG 238 Query: 251 VRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAIFGLLTVWLLLHVRQELR 310 R LYTF R+PG+ VF+G IF+ W YAVL +A F + +++L+ VR EL+ Sbjct: 239 PRWLYTFARVPGTGWTVFIGRTGARIFREWYNMLYAVLFLMASFCIASIYLIRRVRVELQ 298 Query: 311 RRVTAERQLEALARTDGLTGLLNRRALDEALQATWERCQRNLGASFSVLFIDVDYFKAYN 370 RR ++QLE LARTD LT LLNRRALDEAL W+R + N A FS+LF+DVD+FK YN Sbjct: 299 RRSALDQQLEELARTDKLTSLLNRRALDEALARAWKRAEENPDAIFSLLFVDVDFFKLYN 358 Query: 371 DTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEEFVVLLEQTDLQGAQMMAQRLCDA 430 DTYGHK GD L V+ + A + R +D +GRYGGEEFVVLL TD GA +A+R+ A Sbjct: 359 DTYGHKAGDYALMAVSRCIVAAVSRLSDQSGRYGGEEFVVLLNGTDTVGATHVAKRIISA 418 Query: 431 VRERHIAHTSSPLAVVTISVGVATLERDHHRRVEDVLNAADAALYRAKREGRN 483 ++ I H SP ++T+S+GV +L R+HH +EDV+ ADAALYRAK++GRN Sbjct: 419 IQSLKIPHERSPFDILTVSIGVTSLLREHHSSIEDVVKDADAALYRAKQDGRN 471 >gi|300310865|ref|YP_003774957.1| response regulator containing a CheY-like receiver domain and a GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 554 Score = 219 bits (558), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 158/484 (32%), Positives = 253/484 (52%), Gaps = 32/484 (6%) Query: 21 ASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNREMLSLSLDTLAYRFQH---PLSAR-R 76 A T +++ + + ++ASNV L + ++ + + L+T+ H SAR R Sbjct: 42 AWTAWRAREVALSESTTDASNVALALIQHAEQSIKEADIVLETVQEHLDHEDRSASARIR 101 Query: 77 LNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQVSSRLGRGK-GAPHYADRAYFIAHRDS 135 +D ++ S ++ +A + V+D GR SS+ + G+P D YF H+ Sbjct: 102 THD-----FMIRSVASLPQLAQLTVVDEQGRTVASSQQRMVQTGSPVQDD--YFQYHQHR 154 Query: 136 PTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVVMALDLAYFRELFEGLALGDE 195 + ++ +PM++ + + + + +S+R+++ DGSFAGV+V +DLAYF+ +E +G Sbjct: 155 RSDRPFIGQPMRSHVTGQ-RTLTVSRRINHVDGSFAGVIVAEIDLAYFQRYYEQFNIGPH 213 Query: 196 GIISLYSDEGIAYMRVPYRETIIGRDVSQTGNFQRLRAMLSEEEGSFFAHATNDGVRRLY 255 G+I G +R P + R + + ++ M GSF + DGV R+ Sbjct: 214 GVILFGLTGGSLILRRPLLADMTDRSPAMSEALRK--HMEQARRGSFAMRSERDGVERIS 271 Query: 256 TFRRIPGSPLIVFVGYAEEAIFKTWRKTFYA------VLVSLAIFGLLTVWLLLHVRQEL 309 F++ P+ V A++ I + W + + +L+++ FG + +L+ R+ Sbjct: 272 CFQQGEAYPIFTVVALAKDDILEDWSRHVWVRGIGVVLLLAVICFGGKRLVVLVAGRERA 331 Query: 310 RRRVTAERQ--------LEALARTDGLTGLLNRRALDEALQATWERCQRNLGASFSVLFI 361 + A RQ LEA ++ DGLT + NRR D AL A R R G S S++ I Sbjct: 332 EAQAIAARQELESLYRTLEAQSQKDGLTDVFNRRYFDAALAAELARLNRT-GESLSLMMI 390 Query: 362 DVDYFKAYNDTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEEFVVLLEQTDLQGAQ 421 DVD+FK +NDTYGH GD L++VAA ++ R D RYGGEEF V+L AQ Sbjct: 391 DVDHFKQFNDTYGHAAGDVCLRQVAAAISTVARRQGDIVARYGGEEFAVILPNCHPGSAQ 450 Query: 422 MMAQRLCDAVRERHIAHTSSPLAVVTISVGVATLERDHHRRVE--DVLNAADAALYRAKR 479 +A R+ AVR IAH SP+ VVTISVGVA+L +E +++++AD ALYRAK Sbjct: 451 GLAHRVVQAVRGLEIAHERSPMGVVTISVGVASLASGKSLLIEPRELIDSADGALYRAKE 510 Query: 480 EGRN 483 EGR+ Sbjct: 511 EGRD 514 >gi|300312843|ref|YP_003776935.1| response regulator containing a CheY-like receiver and a GGDEF domains protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 204 bits (518), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 147/383 (38%), Positives = 213/383 (55%), Gaps = 24/383 (6%) Query: 124 ADRAYFIAHRDSPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVVMALDLAYF 183 +DR YFI HRD VG +V + + S ++ +S+RL++ DGSFAGV++ LD+ +F Sbjct: 137 SDRDYFIWHRDHDDVGPHVGKAL-LSRSTGRWIIPMSRRLNHPDGSFAGVLLATLDINHF 195 Query: 184 RELFEGLALGDEGIISLYSDEGIAYMRVPYRETIIGRDVSQTGNFQRLRAMLSEEEGSFF 243 +L+ + +G G I+L +G R+P+ I RD SQ F L + G Sbjct: 196 NQLYRTVEIGKGGSIALLLRDGTLLTRMPFDPGYINRDFSQGRLF--LEGLPHSPFGYLE 253 Query: 244 AHATNDGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFY----AVLVSLAIFGLLTV 299 + D +R +++ + PL++ V A E W++ + AVL+ LAI G+ Sbjct: 254 VVSPLDARQRFLSYQAVDKYPLVLVVTLAREEALADWKQQTFVYGAAVLLLLAIIGVFGW 313 Query: 300 WLLLHVRQELRRRV------------TAERQLEALARTDGLTGLLNRRALDEALQATWER 347 L+ + ELR R A +LE LA DGLTGL NRR LD L+ ++R Sbjct: 314 RLVGQI--ELRLRAEDRALQAMGELQMANHRLEMLAHQDGLTGLANRRHLDTMLETEFKR 371 Query: 348 CQRNLGASFSVLFIDVDYFKAYNDTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEE 407 +R+ GA+ +++ IDVD+FK YND YGH+ GD L+ VA L R R D A RYGGEE Sbjct: 372 AERS-GAALALIMIDVDFFKQYNDLYGHQAGDECLRRVAGVLKERQRRPGDLAARYGGEE 430 Query: 408 FVVLLEQTDLQGAQMMAQRLCDAVRERHIAHTSSPLAVVTISVGVATLE--RDHHRRVED 465 +LL TD +GA +A++L ++ + H +P+ VVT+SVGV LE R V+ Sbjct: 431 MAMLLPGTDAEGACAVAEKLRAGIQALELPHRGNPVGVVTVSVGVCALELLPSAARSVQA 490 Query: 466 VLNAADAALYRAKREGRNAVRVS 488 +L AADAALY AK EGRN VR++ Sbjct: 491 LLGAADAALYEAKHEGRNQVRLA 513 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,395,509 Number of extensions: 58480 Number of successful extensions: 322 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 229 Number of HSP's successfully gapped: 40 Length of query: 491 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 402 Effective length of database: 1,198,455 Effective search space: 481778910 Effective search space used: 481778910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)