BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0343.p (429 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313917|ref|YP_003778009.1| two component sensor histidine ... 840 0.0 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 78 2e-15 gi|300312426|ref|YP_003776518.1| nitrogen regulation (sensor his... 74 2e-14 gi|300310677|ref|YP_003774769.1| two component transmembrane sen... 72 6e-14 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 72 6e-14 >gi|300313917|ref|YP_003778009.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 517 Score = 840 bits (2171), Expect = 0.0, Method: Compositional matrix adjust. Identities = 426/429 (99%), Positives = 426/429 (99%) Query: 1 VLAFLLWQLSRQGASAQIGQATGQATISCAAMSAGLSHALGAASTPGQDAPAPTPVMQAV 60 VLAFLLWQLSRQGASAQIGQATGQATISCAAMSAGLSHALGAASTPGQDA APTPVMQAV Sbjct: 89 VLAFLLWQLSRQGASAQIGQATGQATISCAAMSAGLSHALGAASTPGQDASAPTPVMQAV 148 Query: 61 IDLALRDQAGVEGGFWQAGKGVVAYAFPTYDGSGVKRDPPGAEMERIDSTAQRALDSKTT 120 IDLALRDQAGVEGGFWQAGKGVVAYAFPTYDGSGVKRDPPGAEMERIDSTAQRALDSKTT Sbjct: 149 IDLALRDQAGVEGGFWQAGKGVVAYAFPTYDGSGVKRDPPGAEMERIDSTAQRALDSKTT 208 Query: 121 ITDVRPGLREAVVFAACPLSVTTDAALAAWTLKRVPVMSAAVLDQLLVAIGLLLAFVVIT 180 ITDVRPGLREAVVFAACPLSVTTDAALAAWTLKRVPVMSAAVLDQLLVAIGLLLAFVVIT Sbjct: 209 ITDVRPGLREAVVFAACPLSVTTDAALAAWTLKRVPVMSAAVLDQLLVAIGLLLAFVVIT 268 Query: 181 GLWLGATLTRWRSQSERLAAQLAHAERLATLGRVAAGMAHEIRNPIGTMRMKAENALAAP 240 GLWLGATLTRWRSQSERLAAQLAHAERLATLGRVAAGMAHEIRNPIGTMRMKAENALAAP Sbjct: 269 GLWLGATLTRWRSQSERLAAQLAHAERLATLGRVAAGMAHEIRNPIGTMRMKAENALAAP 328 Query: 241 PQMREARATGALEMVVAQTERLDALVGSLLALSQPFQLQRQPLDLAQWLPQRRLAHLEMA 300 PQMREARAT ALEMVVAQTERLDALVGSLLALSQPFQLQRQPLDLAQWLPQRRLAHLEMA Sbjct: 329 PQMREARATSALEMVVAQTERLDALVGSLLALSQPFQLQRQPLDLAQWLPQRRLAHLEMA 388 Query: 301 ERQGVRIQVTLPEQDDLVTHSLDPAQLARALDNLLLNALAHCPAGGEIEIGARLRSPGRL 360 ERQGVRIQVTLPEQDDLVTHSLDPAQLARALDNLLLNALAHCPAGGEIEIGARLRSPGRL Sbjct: 389 ERQGVRIQVTLPEQDDLVTHSLDPAQLARALDNLLLNALAHCPAGGEIEIGARLRSPGRL 448 Query: 361 LLWVADNGPGVPQDLRQSLFDPFVSQRPGGTGLGLVLVREIIHAHGGEIILAPTAAGARF 420 LWVADNGPGVPQDLRQSLFDPFVSQRPGGTGLGLVLVREIIHAHGGEIILAPTAAGARF Sbjct: 449 FLWVADNGPGVPQDLRQSLFDPFVSQRPGGTGLGLVLVREIIHAHGGEIILAPTAAGARF 508 Query: 421 EMELPWRAS 429 EMELPWRAS Sbjct: 509 EMELPWRAS 517 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 77.8 bits (190), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 71/239 (29%), Positives = 109/239 (45%), Gaps = 36/239 (15%) Query: 205 AERLATLGRVAAGMAHEIRNPIGTMRMKAENALAAPPQMREARATGALEMVVAQTERLDA 264 ++ +A R A AH+++ P+ MRM++E AL Q+ R +LE + +E Sbjct: 143 SQTIAIQKRFIADAAHQMKTPLAGMRMQSELALRQDDQVDVRR---SLEQLAKSSETATR 199 Query: 265 LVGSLLALSQPFQLQRQ-----PLDLAQ--------WLPQRRLAHLEMAERQGVRIQVTL 311 LV LL+L++ Q Q P++L + W+P M+ Q + + Sbjct: 200 LVNQLLSLARAENQQSQSGGMIPVELNELARSVVQDWVP--------MSFTQRIDLGFEQ 251 Query: 312 PEQDDLVTHSLDPAQLARALDNLLLNALAHCPAGGEIEIGARLRSP---GRLLLWVADNG 368 P+ L+T +P L L NLL NAL + P G + R R+ G +L V DNG Sbjct: 252 PDHPILITG--NPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGGGLAILEVEDNG 309 Query: 369 PGVPQDLRQSLFDPFVS-----QRPGGTGLGLVLVREIIHAHGG--EIILAPTAAGARF 420 PG+P R ++F+ F G GLGL +VREI H EI P + ++ Sbjct: 310 PGIPVAERANVFERFYRILGTPSTGSGLGLGLAIVREIAQQHEAALEITSNPRSTDPKY 368 >gi|300312426|ref|YP_003776518.1| nitrogen regulation (sensor histidine kinase) transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 366 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 69/229 (30%), Positives = 106/229 (46%), Gaps = 28/229 (12%) Query: 217 GMAHEIRNPIGTMRMKAENALAAPPQMREARATGALEMVVAQTERLDALVGSLLALSQPF 276 +AHEI+NP+G +R A+ P+ ++++ + +RL LV LLA P Sbjct: 146 NLAHEIKNPLGGIRGAAQLLELELPERHLKELREYTQVIIKEADRLQTLVDRLLA---PH 202 Query: 277 QLQRQPLDLAQWLPQRRLAHLEMAER-QGVRIQ----VTLPEQDDLVTHSLDPAQLARAL 331 + D+ R+ L MAE QG+ I+ +++PE D QL +A+ Sbjct: 203 RHPHIVGDVNIHEVCERVRSLVMAEFPQGLTIKRDYDLSIPE------FRGDKEQLIQAV 256 Query: 332 DNLLLNA---LAHCPAGGEIEIGARLRSPGR-----------LLLWVADNGPGVPQDLRQ 377 N++ NA LA G+ E+ R R + L L + DNGPG+P D++ Sbjct: 257 LNIVHNAAQALADRIRAGDGELILRTRVSRQVTLSKVRYRLALDLHIIDNGPGIPPDIQD 316 Query: 378 SLFDPFVSQRPGGTGLGLVLVREIIHAHGGEIILAPTAAGARFEMELPW 426 +F P VS R GG+GLGL L + +H H G I F + +P Sbjct: 317 RIFYPLVSGREGGSGLGLTLAQTFVHQHMGVIECDSRPGCTDFRILIPL 365 >gi|300310677|ref|YP_003774769.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 72.4 bits (176), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 75/264 (28%), Positives = 116/264 (43%), Gaps = 17/264 (6%) Query: 171 GLLLAFVVITGLWLGATLTRWRSQSERLAAQLAHAERLATLGRVAAGMAHEIRNPIGTMR 230 G LL F IT + A R R Q A L + R +G +A +AHE+ P+ +R Sbjct: 247 GHLLHFHDITD-QVEAEEARRRDQ-----AALEYLARHNAMGEMATAIAHELSQPLAAIR 300 Query: 231 MKAENA---LAAPPQMRE--ARATGALEMVVAQTERLDALVGSLLALSQPFQLQRQPLDL 285 E + L +RE + LE Q + A++ S+ + +DL Sbjct: 301 NFLEGSVLRLNHSEALREQAGQIIWGLESAGRQVDHAAAIIKSVREYVVKLEQSEGLIDL 360 Query: 286 AQWLPQRRLAHLEMAERQGVRIQVTLPEQDDLVTHSLDPAQLARALDNLLLNA---LAHC 342 + L + R +EM +G R+Q+ + ++ + S + + + + NL NA ++H Sbjct: 361 NEVLAEVRW-FIEMRAAEG-RVQLEVQASEEALVVSCEKVLIGQVILNLAFNAIEEMSHF 418 Query: 343 PAGGEIEIGARLRSPGRLLLWVADNGPGVPQDLRQSLFDPFVSQRPGGTGLGLVLVREII 402 P + R + LL VAD G G+ R+ LFD F S + G G+GL L + II Sbjct: 419 PENQRRVCISLARKDQQALLTVADEGRGIDAGHRERLFDGFFSSKVSGNGIGLALCKNII 478 Query: 403 HAHGGEIILAPTAA-GARFEMELP 425 H G+I P G RF LP Sbjct: 479 ARHRGDIWAQPAQVRGTRFCFTLP 502 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 72.4 bits (176), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 67/224 (29%), Positives = 109/224 (48%), Gaps = 20/224 (8%) Query: 196 ERLAAQLAHAERLATLGRVAAGMAHEIRNPIGTMRMKAENALAAPPQMREARATGALEMV 255 +LA L E+L A ++HE+R P+ + + E AL +A+ +L+ Sbjct: 235 NQLAMTLERNEKLRR--EFMADVSHELRTPLAILNGQLE-ALEDGVLQPDAQTLRSLK-- 289 Query: 256 VAQTERLDALVGSLLALS----QPFQLQRQPLDLAQWLPQRRLAHLEMAERQGVRIQVTL 311 ++ E L+ LVG L LS +R +DLA L + + ++G+R+ L Sbjct: 290 -SEVEHLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCEL 348 Query: 312 PEQDDLVTHSLDPAQLARALDNLLLNALAHCPAGGEIEIGARLRSPGRLLLWVADNGPGV 371 P + H D A+L + L NL N+L + AGG +++ A L+ L++ D+ PGV Sbjct: 349 PAS--MPVHG-DAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVF-DDSEPGV 404 Query: 372 PQDLRQSLFDPFV------SQRPGGTGLGLVLVREIIHAHGGEI 409 P ++ LF+ F ++ GG GLGL + R I+ AH G I Sbjct: 405 PPEVMARLFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTI 448 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,414,206 Number of extensions: 62134 Number of successful extensions: 368 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 330 Number of HSP's successfully gapped: 45 Length of query: 429 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 341 Effective length of database: 1,203,190 Effective search space: 410287790 Effective search space used: 410287790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)