BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0340.p (252 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum ... 508 e-145 gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogena... 78 7e-16 gi|300313264|ref|YP_003777356.1| short-chain alcohol dehydrogena... 76 2e-15 gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogena... 75 6e-15 gi|300313636|ref|YP_003777728.1| short-chain alcohol dehydrogena... 74 1e-14 >gi|300313914|ref|YP_003778006.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 508 bits (1309), Expect = e-145, Method: Compositional matrix adjust. Identities = 252/252 (100%), Positives = 252/252 (100%) Query: 1 MQLTDNTIFITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVVAANPGMQAIAFDVA 60 MQLTDNTIFITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVVAANPGMQAIAFDVA Sbjct: 1 MQLTDNTIFITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVVAANPGMQAIAFDVA 60 Query: 61 DPASIDAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSA 120 DPASIDAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSA Sbjct: 61 DPASIDAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSA 120 Query: 121 LIDLLKQQPRATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQEIA 180 LIDLLKQQPRATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQEIA Sbjct: 121 LIDLLKQQPRATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQEIA 180 Query: 181 PPWVDTDLVKKSGDPRAMPLDAFITETMARLASEDEEIIVDAVKPLRDNPGAKEHALVNG 240 PPWVDTDLVKKSGDPRAMPLDAFITETMARLASEDEEIIVDAVKPLRDNPGAKEHALVNG Sbjct: 181 PPWVDTDLVKKSGDPRAMPLDAFITETMARLASEDEEIIVDAVKPLRDNPGAKEHALVNG 240 Query: 241 FNAALMAHPIPV 252 FNAALMAHPIPV Sbjct: 241 FNAALMAHPIPV 252 >gi|300310554|ref|YP_003774646.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 77.8 bits (190), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 53/176 (30%), Positives = 89/176 (50%), Gaps = 2/176 (1%) Query: 8 IFITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVVA--ANPGMQAIAFDVADPASI 65 + ITG +G G +A F G+ VI GRR+ LD++V + + DV +SI Sbjct: 3 VLITGATAGFGAAMARKFAGNGHKVIAAGRRKERLDDLVKELGADKVLPLVLDVTSKSSI 62 Query: 66 DAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSALIDLL 125 + A+L + ++VL+NNAG+ + + ++DTN+ G + +T AL+ + Sbjct: 63 ETGLASLTGAWKEVDVLINNAGLALGLGPAHEVALSDWETMIDTNVKGLVTITHALLPDM 122 Query: 126 KQQPRATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQEIAP 181 ++ R TI++ SV P VY A+KA + + ++ R L T VR +AP Sbjct: 123 VKRGRGTIINLGSVAGAYPYPGGNVYGATKAFVDQFTLNLRADLVGTGVRATNLAP 178 >gi|300313264|ref|YP_003777356.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 76.3 bits (186), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 53/156 (33%), Positives = 79/156 (50%), Gaps = 6/156 (3%) Query: 9 FITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVV----AANPGMQAIAFDVADPAS 64 +TG SGIGR + A + G V + GRRQ L+E V P +A DV DPAS Sbjct: 12 LVTGAGSGIGRQITLALLREGYGVALAGRRQDALEETVRLAGEDGPNALVVAADVTDPAS 71 Query: 65 IDAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSALIDL 124 + A + R+ L++L NNAGI + + E + +D NL G + + Sbjct: 72 VKQLFAKTRERFGRLDMLFNNAGIFAPPVSLEELSVEQWKAAVDINLTGVFLCSQEAFRI 131 Query: 125 LK-QQPR-ATILHNTSVLAYVPLAVTAVYSASKAAL 158 +K Q PR I++N S+ A+ P +A Y+A+K A+ Sbjct: 132 MKDQSPRGGRIINNGSISAHAPRPFSASYTATKHAI 167 >gi|300311759|ref|YP_003775851.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 75.1 bits (183), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 63/178 (35%), Positives = 95/178 (53%), Gaps = 11/178 (6%) Query: 6 NTIFITGGASGIGRGLAEAFHKLGNTVIIGGR--RQALLDEVVAANPGMQAIAFDVADPA 63 TIFITG +SG G +A F + VI R R LL A+P ++ + DVAD Sbjct: 2 KTIFITGCSSGFGLEIARYFLQRDWKVIATMRTPRHDLLP----ASPHLRILPLDVADAH 57 Query: 64 SIDAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSALID 123 SI AA ++A P ++VLVNNAGI +V R + +TN LG I +T A I Sbjct: 58 SIQAA---VEAAGP-VDVLVNNAGIGNLGALEAQPMAQV-RELFETNTLGSIAMTQAFIP 112 Query: 124 LLKQQPRATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQEIAP 181 ++ + + +++ TS + L + AVY+ASKAA++++ S L V+V+ + P Sbjct: 113 QMRARGQGLVINVTSSVTLRALPLLAVYTASKAAINAFTESLALELAPLGVQVRLVLP 170 >gi|300313636|ref|YP_003777728.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 268 Score = 74.3 bits (181), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 65/206 (31%), Positives = 98/206 (47%), Gaps = 10/206 (4%) Query: 8 IFITGGASGIGRGLAEAFHKLGNTVIIGGRRQALLDEVVAANPGMQ---AIAFDVADPAS 64 +F+TG +SG+G LA A+ G + + RR A L+ + AA P + A DV D A+ Sbjct: 13 VFLTGASSGLGMALARAYASQGAVLGLVARRTAELEALRAALPHAERHRVYALDVTDHAA 72 Query: 65 IDAAAATLKARYPGLNVLVNNAGIMPFDDASGHIDDEVARRILDTNLLGPIRLTSALIDL 124 + AAA + + G +V++ NAGI D RIL N+ + + Sbjct: 73 LAAAATSFMDEFGGADVVIANAGISQGTLTEFQEDLAAFERILAVNVTATFATFTPFVAR 132 Query: 125 LKQQP-----RATILHNTSVLAYVPLAVTAVYSASKAALHSYAMSQRFMLRETSVRVQEI 179 +K P R ++ SV L YSASK+A+ SY S R LR + ++V I Sbjct: 133 MKASPEAQRRRCRLVGIGSVAGIRGLPGAGAYSASKSAVISYCESLRVELRASGIKVVTI 192 Query: 180 APPWVDTDL--VKKSGDPRAMPLDAF 203 AP ++DT + V P MP++ F Sbjct: 193 APGYIDTPMTRVNTYRMPFLMPVERF 218 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 751,407 Number of extensions: 28467 Number of successful extensions: 217 Number of sequences better than 1.0e-08: 34 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 34 Length of query: 252 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 169 Effective length of database: 1,226,865 Effective search space: 207340185 Effective search space used: 207340185 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)