BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0339.p (270 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herb... 536 e-154 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 86 4e-18 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 83 3e-17 gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogena... 82 4e-17 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 80 2e-16 >gi|300313913|ref|YP_003778005.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 270 Score = 536 bits (1381), Expect = e-154, Method: Compositional matrix adjust. Identities = 268/270 (99%), Positives = 269/270 (99%) Query: 1 MNQPHTPAHRGTALVTGASSGIGAIYADRLARLGYDLILVARNRSRLNTLAERITNQTGR 60 MNQPHTPAHRGTALVTGASSGIGAIYADRLARLGYDLILVARNRSRLNTLAERITNQTGR Sbjct: 1 MNQPHTPAHRGTALVTGASSGIGAIYADRLARLGYDLILVARNRSRLNTLAERITNQTGR 60 Query: 61 SVEVLEADLGQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSDVDHMTGMIALNVT 120 SVEVLEADLGQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSDV+HMTGMIALNVT Sbjct: 61 SVEVLEADLGQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSDVEHMTGMIALNVT 120 Query: 121 APTRLTYAAVPGFVARGQGAVINISSIVGVAPEVLNGVYGGSKAFVLAFSQSLHHELAAQ 180 APTRLTYAAVPGFVARGQGAVINISSIVGVAPEVLNGVYGGSKAFVLAFSQSLHHELAAQ Sbjct: 121 APTRLTYAAVPGFVARGQGAVINISSIVGVAPEVLNGVYGGSKAFVLAFSQSLHHELAAQ 180 Query: 181 GVQVQAVLPGATATDFWAIGGLPVEHLDPAIVMSAEQMVDAALVGFQRGELVTIPSLHEE 240 GVQVQAVLPGATATDFWAIGGLPVEHLDPAIVMSAEQMVDAALVGFQR ELVTIPSLHEE Sbjct: 181 GVQVQAVLPGATATDFWAIGGLPVEHLDPAIVMSAEQMVDAALVGFQRRELVTIPSLHEE 240 Query: 241 DAWTGYEAARQALIARLSANTPAPRYRAAH 270 DAWTGYEAARQALIARLSANTPAPRYRAAH Sbjct: 241 DAWTGYEAARQALIARLSANTPAPRYRAAH 270 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 85.5 bits (210), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 66/197 (33%), Positives = 101/197 (51%), Gaps = 7/197 (3%) Query: 13 ALVTGASSGIGAIYADRLARLGYDLILVARNRSRLNTLAERITNQTGRSVEVLEADLGQA 72 AL+TGAS+GIG A AR G LIL AR + L +A I Q GR+ V+ D+G+A Sbjct: 9 ALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRA-SVVAGDVGEA 67 Query: 73 ASLAQ-VEEKLRSDASITLLVNNAG-IGTHTPLLQSDVDHMTGMIALNVTAPTRLTYAAV 130 A+ + V L + + +NNAG +G PL + + N+T+ + Sbjct: 68 ATHERGVAMALDEFGGLDIAINNAGAVGACKPLAEISPQQWQDTLHANLTSAFLGARCQI 127 Query: 131 PGFVARGQGAVINISSIVGVAPEVLN-GVYGGSKAFVLAFSQSLHHELAAQGVQVQAVLP 189 P +ARG GA++ SS VG + + YG +KA ++ + + + A QG++ A+LP Sbjct: 128 PAMLARGAGAIVFTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYAVQGIRANALLP 187 Query: 190 GATATDFWAIGGLPVEH 206 G TD +GG P + Sbjct: 188 GGVDTD---MGGDPQQK 201 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 82.8 bits (203), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 68/189 (35%), Positives = 100/189 (52%), Gaps = 9/189 (4%) Query: 13 ALVTGASSGIGAIYADRLARLGYDLILVARNRSRLNTLAERITN---QTGRSVEVLEADL 69 ALVTGAS GIG A LAR G +I A + S + E + + GR VL + Sbjct: 11 ALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGA-VLNVND 69 Query: 70 GQAASLAQVEEKLRSDASITLLVNNAGIGTHTPLLQSDVDHMTGMIALNVTAPTRLTYAA 129 +A S+A VE+ + S+++LVNNAGI ++ + +IA N+TA RL+ A Sbjct: 70 AEA-SVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAV 128 Query: 130 VPGFVARGQGAVINISSIVGVA--PEVLNGVYGGSKAFVLAFSQSLHHELAAQGVQVQAV 187 + G + G +INI+S+VG A P +N Y +KA V S++L E+ ++ + V V Sbjct: 129 LRGMMKARTGRIINITSVVGEAGNPGQMN--YAAAKAGVAGMSRALAREIGSRNITVNCV 186 Query: 188 LPGATATDF 196 PG TD Sbjct: 187 APGFIDTDM 195 >gi|300309640|ref|YP_003773732.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 82.4 bits (202), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 66/187 (35%), Positives = 97/187 (51%), Gaps = 8/187 (4%) Query: 14 LVTGASSGIGAIYADRLARLGYDLILVARNRSRLNTLAERITNQTGRSVEVLEADLGQAA 73 ++TGASSG+G A LA LG L+L AR RL L IT G+++ V D+ + Sbjct: 11 VITGASSGLGETTARHLASLGAKLVLGARRTERLEKLVADITAAGGQAIAVT-TDVARRD 69 Query: 74 SL-AQVEEKLRSDASITLLVNNAGIGTHTPLLQSDVDHMTGMIALNVTAPTRLTYAAVPG 132 + A V + + I +LVNNAGI P+ + V+ MI +NV AA+P Sbjct: 70 DVEALVAKGEQHFGRIDVLVNNAGIMPLAPMAKLKVEEWDRMIDVNVKGVLYGVAAALPR 129 Query: 133 FVARGQGAVINISSIVGVAPEVLNG---VYGGSKAFVLAFSQSLHHELAAQGVQVQAVLP 189 F A+ G +IN+SS+ G+ +V G VY +K V A ++ L E A GV+ + P Sbjct: 130 FAAQSSGHIINVSSVAGI--KVFAGMGTVYSATKFAVRALTEGLRTE-APDGVRTTIISP 186 Query: 190 GATATDF 196 GA ++ Sbjct: 187 GAVDSEL 193 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 60/207 (28%), Positives = 92/207 (44%), Gaps = 13/207 (6%) Query: 13 ALVTGASSGIGAIYADRLARLGYDLIL-VARNRSRLNTLAERITNQTGRSVEVLEADLGQ 71 A VTG GIG RL + GY ++ N +R + + Q G + E ++ Sbjct: 6 AYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQ-GFDIHASEGNVSN 64 Query: 72 AASLAQVEEKLRSD-ASITLLVNNAGIGTHTPLLQSDVDHMTGMIALNVTAPTRLTYAAV 130 S EK+R++ + +LVNNAGI + D +I N+ + +T + Sbjct: 65 WESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVTKQVI 124 Query: 131 PGFVARGQGAVINISSIVGVAPEVLNGVYGGSKAFVLAFSQSLHHELAAQGVQVQAVLPG 190 G RG G +INISS+ G + Y +KA + F+ +L E+A +GV V V PG Sbjct: 125 EGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPG 184 Query: 191 ATATDF----------WAIGGLPVEHL 207 TD + G+PV+ L Sbjct: 185 YIGTDMVRAIRPEVLEKIVSGIPVKRL 211 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 860,106 Number of extensions: 36414 Number of successful extensions: 269 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 232 Number of HSP's successfully gapped: 41 Length of query: 270 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 186 Effective length of database: 1,222,130 Effective search space: 227316180 Effective search space used: 227316180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)