BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0325.p (406 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313899|ref|YP_003777991.1| RND family efflux transporter M... 811 0.0 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 107 2e-24 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 105 7e-24 gi|300310851|ref|YP_003774943.1| RND family efflux transporter M... 100 3e-22 gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 87 2e-18 >gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 406 Score = 811 bits (2094), Expect = 0.0, Method: Compositional matrix adjust. Identities = 405/406 (99%), Positives = 406/406 (100%) Query: 1 MSHDRHSELGIHPLSEGEPQQRQQMMRRARVVLVVVLLLLAAGAARTVISRVANARELQA 60 MSHDRHSELGIHPLSEGEPQQRQQMMRRARVVLVVVLLLLAAGAARTVISRVANARELQA Sbjct: 1 MSHDRHSELGIHPLSEGEPQQRQQMMRRARVVLVVVLLLLAAGAARTVISRVANARELQA 60 Query: 61 GTAERAKMFVRVAPVRSSAEGQTLALPGTLQGFVQSPISARSGGYLKRWTKDIGAEVKKG 120 GTAERAKMFVRVAPVRSSAEGQTLALPGTLQGFVQSPISARSGGYLKRWTKDIGAEVKKG Sbjct: 61 GTAERAKMFVRVAPVRSSAEGQTLALPGTLQGFVQSPISARSGGYLKRWTKDIGAEVKKG 120 Query: 121 ELLAELDTPEIDQQLTQALAAREQAASSMELARSSMARWEALRKKDAVSQQELDERRSAF 180 ELLAELDTPEIDQQLTQALAAREQAASSMELARSSMARWEALRKKDAVSQQELDERRSAF Sbjct: 121 ELLAELDTPEIDQQLTQALAAREQAASSMELARSSMARWEALRKKDAVSQQELDERRSAF 180 Query: 181 AQASANLAAADANVGRLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTD 240 AQASANLAAADANVGRLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTD Sbjct: 181 AQASANLAAADANVGRLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTD 240 Query: 241 PLRVYVNVPQSYANLVKPGQQVTVTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSN 300 PLRVYVNVPQSYANLVKPGQQVTVTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSN Sbjct: 241 PLRVYVNVPQSYANLVKPGQQVTVTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSN 300 Query: 301 ADHALLPGAFVQVALPIKPSGALLVAADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFG 360 ADHALLPGAFVQVALPIKPSGALLVAADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFG Sbjct: 301 ADHALLPGAFVQVALPIKPSGALLVAADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFG 360 Query: 361 QTVEVLDGLQGDEHLILNPSDALAEGDQVEPTLEQPKEPEKKKAAS 406 QTVEVLDGLQGDEHLILNPSDALAEGDQVEPTLEQPKEPEK+KAAS Sbjct: 361 QTVEVLDGLQGDEHLILNPSDALAEGDQVEPTLEQPKEPEKQKAAS 406 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 99/344 (28%), Positives = 164/344 (47%), Gaps = 21/344 (6%) Query: 69 FVRVAPVRSSAEGQTLALPGTLQGFVQSPISARSGGYLKRWTKDIGAEVKKGELLAELDT 128 V +AP R + T LPG ++ F + + AR G +++ G+EVK+G++L ++D Sbjct: 41 VVTIAPQRVAI---TNELPGRVEAFRVAQVRARVAGIVQKRVFKEGSEVKEGQVLFKIDP 97 Query: 129 PEIDQQLTQALAAREQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASANLA 188 + L A A +A +++ + R++ L + +AVS+QE D +A QA A++A Sbjct: 98 AQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQAEADVA 157 Query: 189 AADANVGRLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNV 248 A A V G+ + +P SG I R V G L+ G L ++ Q DP VYVNV Sbjct: 158 AGKAAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQGDATQLALVQQLDP--VYVNV 215 Query: 249 PQSYANLVKPGQ-------------QVTVTQSELRGRKF--EGKVARTAGAIDAATRSMQ 293 QS A+L+K Q + +VT G+ + GK+ + ++D T S+ Sbjct: 216 TQSSADLLKLQQAMAAGQLKSVGDNKASVTLELENGQAYPLPGKLLFSDISVDENTGSVS 275 Query: 294 VEVTLSNADHALLPGAFVQVALP-IKPSGALLVAADTLMFRRDGTLVAAVDPHNQVRLKK 352 + N LLPG +V+ + ALLV ++ G+ V V+ N+V + Sbjct: 276 LRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLVPQQAVVRDAGGSTVMVVNAENKVEPRP 335 Query: 353 LRLGRNFGQTVEVLDGLQGDEHLILNPSDALAEGDQVEPTLEQP 396 ++ G G +VLDGL+ + +I+ + G V+P P Sbjct: 336 VKTGGARGNNWQVLDGLKEGDRVIVEGLQKVRPGATVKPVQWNP 379 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 105 bits (262), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 92/338 (27%), Positives = 163/338 (48%), Gaps = 12/338 (3%) Query: 70 VRVAPVRSSAEGQTLALPGTLQGFVQSPISARSGGYLKRWTKDIGAEVKKGELLAELDTP 129 V +A V+ Q LA GT+ + R G L++ G +VKKG+LLA++D Sbjct: 51 VTLASVQERDLPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGEDVKKGQLLAQIDAR 110 Query: 130 EIDQQLTQALAAREQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASANLAA 189 + QL QA A R + ++++ AR + R+ LR +DA +QQ+LD +++ AQ A + Sbjct: 111 ALQAQLAQAEAQRAKDSATLMNARLDLQRYTTLRSQDAATQQQLDTQKALVAQLEAAVKT 170 Query: 190 ADANVGRLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNVP 249 +A V + + I AP SG + R VD G+++ L V++Q DP+ + +P Sbjct: 171 DEAQVAYAKVQLSYTTINAPISGRVGARLVDPGNIVHATDANGLVVINQIDPITLVFTLP 230 Query: 250 QSYANLVKPGQ-------QVTVTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSNAD 302 + + Q QV+ + + GK+ I+ ++Q++ N D Sbjct: 231 EEAVAGINRAQRAHQQPLQVSAYARDSQEVLATGKLVLLDNQINTTNGTVQLKARFDNPD 290 Query: 303 HALLPGAFVQVALPIKPSG-ALLVAADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFGQ 361 H L PG +V L ++ G AL V A + + GT V A+D +Q R++ +++ R Sbjct: 291 HKLWPGQYVNARLQMRSHGNALTVPAAAIQRNQQGTYVYAIDQEDQARMQPVKVER-IQD 349 Query: 362 TVEVL---DGLQGDEHLILNPSDALAEGDQVEPTLEQP 396 + VL + +Q + ++++ L G ++ + QP Sbjct: 350 GIAVLAAGNAVQAGQRVVIDGQYKLKPGSRIVESKPQP 387 >gi|300310851|ref|YP_003774943.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 100 bits (248), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 76/302 (25%), Positives = 139/302 (46%), Gaps = 11/302 (3%) Query: 98 ISARSGGYLKRWTKDIGAEVKKGELLAELDTPEIDQQLTQALAAREQAASSMELARSSMA 157 + AR G L + G VK G+LLAE+D + LTQA + + + AR + Sbjct: 62 VRARVDGQLMKLHYKEGQVVKAGDLLAEIDPRSLQAALTQAEGQLARDRALLASARLDLK 121 Query: 158 RWEALRKKDAVSQQELDERRSAFAQASANLAAADANVGRLRQVEGFKRIVAPFSGIITRR 217 R++ L +D+++ Q++D + + Q + A + NV R F R+ AP SG + R Sbjct: 122 RYQTLLAQDSIASQQVDTQVALVKQYEGTVKADEGNVASARLQLSFTRVTAPISGRLGLR 181 Query: 218 NVDVGDLIDPGAGRALFVLSQTDPLRVYVNVPQSYANLVKPGQQVTVTQS--------EL 269 DVG+ + L +++Q P+ ++P+ N+ K QQ+ + E Sbjct: 182 QADVGNNVTTSDTNGLVIITQLQPITAIFSIPED--NIPKVLQQLQSGRKLPAQAWDREQ 239 Query: 270 RGRKFEGKVARTAGAIDAATRSMQVEVTLSNADHALLPGAFVQVALPIKPS-GALLVAAD 328 + + +G + +DA T +++++ N D+AL P FV + L + GA ++ Sbjct: 240 KNKLADGVLLTIDNVVDATTGTVKLKAQFPNTDYALFPSQFVNIRLQLNTEQGATVIPTA 299 Query: 329 TLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFGQTVEVLDGLQGDEHLILNPSDALAEGDQ 388 + G V V + V ++ ++ G + DG+ E ++++ D L EG + Sbjct: 300 AIQRGSKGLFVYVVKDDSSVTVRPVKTGPVQDDLTVITDGVSAGETVVIDGIDRLREGAK 359 Query: 389 VE 390 VE Sbjct: 360 VE 361 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 87.0 bits (214), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 85/308 (27%), Positives = 146/308 (47%), Gaps = 25/308 (8%) Query: 103 GGYLKRWTKDIGAEVKKGELLAELDTPEIDQQLTQALAAREQAASSMELARSSMARWEAL 162 GG L GA VKKG++L +D + ++ +A A A + S +AR + L Sbjct: 84 GGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAEAQLAGAEARAGYTASDLARGQRL 143 Query: 163 RKKDAVSQQELDERRSAFAQASANLAAADANVGRLRQVEGFKRIVAPFSGIITRRNVDVG 222 +A+++++ +E+++A +ASAN+AAA A + R + RI AP SG I+R V VG Sbjct: 144 LSDNAIAKRDFEEKQNASREASANVAAARAALRAARLNLEYTRITAPVSGRISRAEVTVG 203 Query: 223 DLIDPGAGRALFVLSQTDPLRVYVNVPQSYANL----------VKPGQQVTVTQ------ 266 ++++PGA A PL V+V + YA+ V P + T Sbjct: 204 NVVNPGAANA--------PLTTLVSVAKVYASFDVDEQSFLKYVNPARAAGATVPVELGL 255 Query: 267 SELRGRKFEGKVARTAGAIDAATRSMQVEVTLSNADHALLPGAFVQVAL-PIKPSGALLV 325 + G +G+V +D A+ +++V NA+ L+PG + +V L +P A+L+ Sbjct: 256 ANEDGYSRKGRVGSVDNRLDTASGTIRVRAIFDNANSQLVPGLYARVRLGGGEPHQAVLI 315 Query: 326 AADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFGQTVEVLDGLQGDEHLILNPSDALAE 385 + +D V D N +++ +G V + GLQ E +++N + Sbjct: 316 DEKAIGTDQDKRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQPGERIVVNGLQRIRP 375 Query: 386 GDQVEPTL 393 GD + PT+ Sbjct: 376 GDAITPTV 383 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.130 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,076,138 Number of extensions: 40894 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 190 Number of HSP's successfully gapped: 10 Length of query: 406 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 319 Effective length of database: 1,207,925 Effective search space: 385328075 Effective search space used: 385328075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)