BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0324.p (486 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 961 0.0 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 288 5e-79 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 281 1e-76 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 276 2e-75 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 216 4e-57 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 961 bits (2485), Expect = 0.0, Method: Compositional matrix adjust. Identities = 485/486 (99%), Positives = 485/486 (99%) Query: 1 MRSRQLLLALACALSLAGCASGPDYTRPQVDMPAAWTPEAPWRSMQPADATPRGPWWERF 60 MRSRQLLLALACALSLAGCASGPDYTRPQVDMPAAWT EAPWRSMQPADATPRGPWWERF Sbjct: 1 MRSRQLLLALACALSLAGCASGPDYTRPQVDMPAAWTSEAPWRSMQPADATPRGPWWERF 60 Query: 61 GDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRP 120 GDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRP Sbjct: 61 GDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRP 120 Query: 121 LTNYNSPNFSTVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAE 180 LTNYNSPNFSTVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAE Sbjct: 121 LTNYNSPNFSTVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAE 180 Query: 181 LATNYFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQI 240 LATNYFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQI Sbjct: 181 LATNYFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQI 240 Query: 241 DLLGRQRAQYEHAIATLTGTPAPAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAAERA 300 DLLGRQRAQYEHAIATLTGTPAPAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAAERA Sbjct: 241 DLLGRQRAQYEHAIATLTGTPAPAFSITPSLTPITPPAIPVGVPSDVLERRPDIAAAERA 300 Query: 301 MAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLR 360 MAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLR Sbjct: 301 MAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLR 360 Query: 361 AGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIAS 420 AGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIAS Sbjct: 361 AGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIAS 420 Query: 421 SRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSRTPPAEAAG 480 SRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSRTPPAEAAG Sbjct: 421 SRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSRTPPAEAAG 480 Query: 481 AGPSRP 486 AGPSRP Sbjct: 481 AGPSRP 486 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 288 bits (738), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 188/477 (39%), Positives = 278/477 (58%), Gaps = 5/477 (1%) Query: 1 MRSRQLLLALACALSLAGCASGPDYTRPQVDM-PAAWTPEAPWRSMQPADATPRGPWWER 59 + + LL L +L+LA C+ GPDY RPQ+D PA + W+ QP D G WWE Sbjct: 7 FKLKPLLAMLVASLALAACSVGPDYVRPQMDTGPAIFKEGQNWKPAQPRDLVADGKWWEM 66 Query: 60 FGDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANR 119 FGD QLNAL Q NQ LA+A A A A + + +A +P ++ +A A+R + Sbjct: 67 FGDPQLNALVAQVDISNQNLALAEANFRSAVALVQSSRSAYSPTLSASAGASRGSSGSAG 126 Query: 120 PLTNYNSPNFST--VQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLL 177 ++ + S V+N + + ++E D +G+V+R VE++ A+A+ SAADL RL Sbjct: 127 ASSSNAASGSSAGSVRNSYTFSLTAAWEADIWGKVRRDVESSQASAQASAADLAAARLSA 186 Query: 178 TAELATNYFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTL 237 A++A NY LR LD + ++ + ++L++A ++ G + D+ Q Q L S Sbjct: 187 QAQVAQNYLQLRVLDAQQQLLEETVQAYERSLQVAQNQYAAGMVAKSDVIQAQTQLKSAQ 246 Query: 238 TQIDLLGRQRAQYEHAIATLTGTPAPAFSITPS-LTPITPPAIPVGVPSDVLERRPDIAA 296 G RAQ EHAIA L G F++ + L + PP +P GVPS +LERRPD+AA Sbjct: 247 ASALDNGVTRAQVEHAIALLVGKTPAQFALPRAPLAAVAPP-VPQGVPSMLLERRPDVAA 305 Query: 297 AERAMAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDG 356 AER +A+ANA+IGVA AAY+PS L S G S +A F P +WSLG +Q++FDG Sbjct: 306 AERRVASANAKIGVAKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQTIFDG 365 Query: 357 GRLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVL 416 G A D + A Y+ TVA+Y++TVLTA QEVED + L LE+ A A+ +AR+ + Sbjct: 366 GARAAATDQAIATYDGTVATYKQTVLTAFQEVEDNLAALRILEQEAAVQGEALQAARQAV 425 Query: 417 EIASSRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSRT 473 + +++Y+ G Y++VITAQ + L++E + ++ +R+ SV L KALGG W R+ Sbjct: 426 TLVNNQYKAGTVSYVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGWDERS 482 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 281 bits (718), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 198/487 (40%), Positives = 278/487 (57%), Gaps = 26/487 (5%) Query: 1 MRSRQLLLALACALSLAGCASGPDYTRPQVDMPAAWTPEAPWRSMQPADATPRGPWWERF 60 R+ + + AL A L GCA GPDY RP +D+P A+ PW+ P PWWE + Sbjct: 29 FRATKGMTALVLAALLGGCAVGPDYARPALDLPQAYKETGPWKLAAPRQIDDHQPWWEAY 88 Query: 61 GDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRP 120 GD LNAL QA A NQT+ A A+ QA+A V A+ P + A A+R++ + N Sbjct: 89 GDPVLNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQAGASRAQTNTN-- 146 Query: 121 LTNYNSPNFSTVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAE 180 + N +++G + S+E D +GR++R EA A A+ SAA L RL + A Sbjct: 147 -------GVQRLGNSYSVGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQAT 199 Query: 181 LATNYFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQI 240 LA +Y LR D++ D+ RR +A ++L+L+T +++ G D+AQ + L + Q+ Sbjct: 200 LAQDYLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQL 259 Query: 241 DLLGRQRAQYEHAIATLTGTPAPAFSITPSLTPI----------------TPPAIPVGVP 284 L R Q EHAIA L G FS+ P+L P + P IPVG+P Sbjct: 260 IDLDDTRNQLEHAIAMLIGKAPAQFSL-PALPPAAASDSAIDANAARLQGSLPQIPVGLP 318 Query: 285 SDVLERRPDIAAAERAMAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWS 344 S++LERRPDIA AER AAANA IGVA AAY+P+ L S G +S +A LFN PS +WS Sbjct: 319 SELLERRPDIANAERLAAAANANIGVARAAYFPTLTLSASGGYNSLAFANLFNTPSRVWS 378 Query: 345 LGVSASQSLFDGGRLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQ 404 LG + ++SLFDGG A D + A Y+A VA Y++TVL +QEVED ++ L L++ Sbjct: 379 LGSALAESLFDGGARSARNDAAVAAYDAAVAQYKQTVLGGLQEVEDKLSTLRVLDQESTV 438 Query: 405 SQLAIASARRVLEIASSRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKA 464 A+ SA+ +A +YE G YL V+T Q + L ++R A L+G++L+ SV LIKA Sbjct: 439 QAQAVQSAQLAERLAMRQYEAGTVTYLSVVTTQAASLTNQRNAVTLLGRQLVASVALIKA 498 Query: 465 LGGDWQS 471 GG WQ+ Sbjct: 499 TGGGWQA 505 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 276 bits (707), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 197/471 (41%), Positives = 266/471 (56%), Gaps = 26/471 (5%) Query: 18 GCASGPDYTRPQVDMPAAWTPEAP----------WRSMQPADATPRGPWWERFGDAQLNA 67 GC+ P Y RP VD PAA+ AP W++ QPA+ RG WW+ FGD +LNA Sbjct: 30 GCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 89 Query: 68 LQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSR---ISANRPLTNY 124 L+ A NQ L A ARL QARA A PQV+ A R R S P Sbjct: 90 LEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPADAD 149 Query: 125 NSPNFSTVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATN 184 PN + +VSYEVD FGRV +V+AA A A++S A + L L A++A Sbjct: 150 TRPN-----TLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQ 204 Query: 185 YFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLG 244 YF +RELD + R + L++++L L R D G S +D+A+ +A L S ++ + Sbjct: 205 YFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGIA 264 Query: 245 RQRAQYEHAIATLTGTPAPAFSITPSLTPITPPAI--PVGVPSDVLERRPDIAAAERAMA 302 RQRA EHA+A L G FS+ P P+T A+ P G+PS +LERRPDIAAAERAMA Sbjct: 265 RQRATAEHALAILLGRTPAEFSLPPQ--PLTRLAVTVPPGLPSALLERRPDIAAAERAMA 322 Query: 303 AANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSL----GVSASQSLFDGGR 358 AANA++GVA +A++PS L G +S LFN S + L G + S LFDGGR Sbjct: 323 AANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGR 382 Query: 359 LRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEI 418 +AGVD ++A YE VASYR TVL A +EVED + L L++ A+A++ R ++ Sbjct: 383 RQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKL 442 Query: 419 ASSRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDW 469 + ++Y GA Y VI A +++L +R + L G+R +V LI+ALGG W Sbjct: 443 SHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 216 bits (549), Expect = 4e-57, Method: Compositional matrix adjust. Identities = 159/486 (32%), Positives = 241/486 (49%), Gaps = 34/486 (6%) Query: 2 RSRQLLLALACALSLAGCASGPDYTRPQVDMPAAWTPEAPWRSMQPADATPRGP------ 55 R + AL A L GC+ P Y RPQ AP S PADA R Sbjct: 11 RLLHMCAALVMAGVLGGCSLAPTYERPQ----------APVESSYPADAAGRAELQAVNL 60 Query: 56 -WWERFGDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSR 114 W + F D +L L AL N+ L A + +ARAQ + A P +NL+ AAR+R Sbjct: 61 GWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTR 120 Query: 115 ISANRPLTNYNSPNFSTVQNDFALGASV-SYEVDFFGRVQRSVEAAGAAAEQSAADLENT 173 +A++ LT T+ N + +G +V +YE+DFFGRV+ +AA A+ + ++ Sbjct: 121 TAASQSLTGQ-----PTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSA 175 Query: 174 RLLLTAELATNYFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALL 233 ++ L A++A Y R +L + + ++ L+LA R D+GA++ LD Q L+ Sbjct: 176 QISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLV 235 Query: 234 DSTLTQIDLLGRQRAQYEHAIATLTGT------PAPAFSITPSLTPITPPAIPVGVPSDV 287 +S L RQRAQ E+A+ L G PA S +T I P G+PSD+ Sbjct: 236 ESARVAKTQLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDI-----PAGLPSDL 290 Query: 288 LERRPDIAAAERAMAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGV 347 L RPDI +AE+++ AANA IG A AA++P L ++G S LF + S WS G Sbjct: 291 LTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGP 350 Query: 348 SASQSLFDGGRLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQL 407 S S +FD GR A +D ++ V +Y + + TA +EV D + +L+ + Sbjct: 351 SLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEA 410 Query: 408 AIASARRVLEIASSRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGG 467 +A+ + L++ R++ G LD + AQ+ L ++++ Q RL ++ L KALGG Sbjct: 411 FLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGG 470 Query: 468 DWQSRT 473 T Sbjct: 471 GLNENT 476 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,210,574 Number of extensions: 42929 Number of successful extensions: 287 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 235 Number of HSP's successfully gapped: 17 Length of query: 486 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 397 Effective length of database: 1,198,455 Effective search space: 475786635 Effective search space used: 475786635 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)