BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0323.p (406 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 575 e-165 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 325 5e-90 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 252 3e-68 gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis pro... 209 4e-55 gi|300313049|ref|YP_003777141.1| methyl-accepting chemotaxis I p... 187 9e-49 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 575 bits (1482), Expect = e-165, Method: Compositional matrix adjust. Identities = 284/285 (99%), Positives = 284/285 (99%) Query: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ Sbjct: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 Query: 61 ASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFS 120 ASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFS Sbjct: 61 ASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFS 120 Query: 121 KSFATYLEIDKELARISGEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD 180 KSFATYLEIDKELARIS EGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD Sbjct: 121 KSFATYLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD 180 Query: 181 MAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRA 240 MAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRA Sbjct: 181 MAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRA 240 Query: 241 TSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGN 285 TSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGN Sbjct: 241 TSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGN 285 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 325 bits (832), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 160/285 (56%), Positives = 213/285 (74%) Query: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 M WF NL IAKKL +SFA VI LT++ GLFAI+ELK VN+AS ++ + W+PT+ AA+ +Q Sbjct: 1 MIWFYNLNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQ 60 Query: 61 ASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFS 120 SLP +R++EL++VTA TP ER A KAIK RLD L +QRA +E ++SE +EK Y +F Sbjct: 61 GSLPPLRLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFL 120 Query: 121 KSFATYLEIDKELARISGEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD 180 K+ Y+++D++LA ++ + +E RALF GDS K +RSM+ N+D I+K+N +GS ++D+ Sbjct: 121 KNLTAYVKVDEQLAEMAANQQYDETRALFNGDSAKLYRSMVGNLDAIVKINTSGSAQADE 180 Query: 181 MAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRA 240 A V+R A WI+GLLAA V+IA LA +VA VSRPL EAV +A+RVA+GDLT+ IR Sbjct: 181 EASTVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIRP 240 Query: 241 TSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGN 285 DETG LM +LRAMN+SL+ IV EVR GTDTITTAS EIA GN Sbjct: 241 AGRDETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGN 285 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 252 bits (644), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 128/285 (44%), Positives = 193/285 (67%) Query: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 M WF +L+I++KLI +F V+ + LG+F+IR+L VN AST+++TNWLP+I+ +Q Sbjct: 1 MKWFYDLRISRKLITTFLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQ 60 Query: 61 ASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFS 120 ++ R+R ELQ + A E E+ + ++ +++ L +A Y QISE EEK +Y + Sbjct: 61 LAMSRLRSFELQHILAKDAKEYEEVERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEVE 120 Query: 121 KSFATYLEIDKELARISGEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD 180 K AT++ ++ +S +GK+EEAR L +G+S K +R+ ++ +D++ VND G+ S+ Sbjct: 121 KLIATFVSEHDKIIALSRQGKDEEARNLQRGESTKAYRTSLQQIDKLAAVNDKGADISNK 180 Query: 181 MAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRA 240 A+ +Y A LWII +AA + +A+ LAV V+R ++RPL EAV+VA+RVA GDLT I Sbjct: 181 QADAIYAQARLWIIVTVAACIGLAMLLAVWVSRLIARPLEEAVSVAQRVAGGDLTTRIEP 240 Query: 241 TSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGN 285 DETG L+ +L++MNDSL IVG+VR GT TITTAS EIA+GN Sbjct: 241 AGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGN 285 >gi|300313024|ref|YP_003777116.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 571 Score = 209 bits (531), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 118/285 (41%), Positives = 170/285 (59%) Query: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 M WF NLKIA KL +F V+V + LG F+ EL AVN++STD+A NWLP+I A + Sbjct: 1 MNWFYNLKIAHKLNAAFLVVLVFIIGLGGFSSLELAAVNKSSTDIARNWLPSIDALSKMS 60 Query: 61 ASLPRMRISELQMVTAATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFS 120 +L R R ++Q + A E + + + ++ L K+ A Y +SE EK LY + Sbjct: 61 LALARSRSFDMQQLLTADKQEEKASRERADKQMATLLKEVARYGKLVSEPREKELYPEIE 120 Query: 121 KSFATYLEIDKELARISGEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDD 180 ++ A Y E + ++ + + +++ A AL S +R + E + E+ +VN G+ SD Sbjct: 121 RNIALYKEAHERMSALFEQDQKQAAHALMNSTSTPVYRKLQEQIGELSEVNSKGAALSDA 180 Query: 181 MAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRA 240 A+ +Y A + I+ L+ VV+A+ L+ +AR V+RPL AV +A +VA GDLT DI Sbjct: 181 DADHIYGQANMAILALVTTCVVLAMALSWWIARLVARPLANAVDIARQVAAGDLTADIPT 240 Query: 241 TSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGN 285 DETG L+ +LR MN +L IV EVR GTDTITTAS EIATGN Sbjct: 241 AHRDETGQLLEALRQMNGNLLNIVREVRQGTDTITTASAEIATGN 285 >gi|300313049|ref|YP_003777141.1| methyl-accepting chemotaxis I protein [Herbaspirillum seropedicae SmR1] Length = 516 Score = 187 bits (476), Expect = 9e-49, Method: Compositional matrix adjust. Identities = 115/294 (39%), Positives = 178/294 (60%), Gaps = 17/294 (5%) Query: 1 MTWFNNLKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQ 60 M WF NLKIA+KL ++F AV+VL ILG+F+I +L V AS++++T+W+P K ++ Sbjct: 1 MGWFKNLKIARKLQLAFLAVLVLMAILGIFSISQLAKVKNASSEISTDWMPAQKKLGDLK 60 Query: 61 ASLPRMRISELQMVTAATPAEREDAL----KAIKSRLDILAKQRAAYEAQISEAEEKVLY 116 R+R +E Q+ E + L +AI + LD K YEA + E+ LY Sbjct: 61 LYFVRLRGTESQLAIYDGDVEAMNGLVSRSQAILTGLDKTMKD---YEASLDNVPERELY 117 Query: 117 EKFSKSFATYLEIDKE-----LARISGEGKEEEARALFKGDSNKTFRSMIENMDEIIKVN 171 K + +L K+ LAR + +G++ LF G SNK + ++ +++++IK+N Sbjct: 118 TKVAGQIGEFLAEHKKIVAAFLARDNPQGRD-----LFLGYSNKLYPQILGDLEKLIKIN 172 Query: 172 DAGSKRSDDMAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQ 231 G + A+ + + + ++ LLAA + I +GLA+ VAR ++RPL +AV +A RVA+ Sbjct: 173 QDGGNAATADADDTFSKSRVGVLVLLAAALAIGMGLALYVARIIARPLQDAVDIARRVAE 232 Query: 232 GDLTMDIRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGN 285 GDL I+ S DETG LM +L MN+ L+++VG+VR+GTD+I TAS EIATGN Sbjct: 233 GDLGAQIQVQSKDETGQLMQALDTMNEGLRKVVGQVRSGTDSIATASTEIATGN 286 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.356 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 994,457 Number of extensions: 36259 Number of successful extensions: 301 Number of sequences better than 1.0e-08: 53 Number of HSP's gapped: 277 Number of HSP's successfully gapped: 53 Length of query: 406 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 319 Effective length of database: 1,207,925 Effective search space: 385328075 Effective search space used: 385328075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)