BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0322.p (484 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313895|ref|YP_003777987.1| two-component system sensor his... 952 0.0 gi|300311272|ref|YP_003775364.1| two component sensor histidine ... 143 2e-35 gi|300313246|ref|YP_003777338.1| two component transmembrane sen... 142 7e-35 gi|300311514|ref|YP_003775606.1| two component sensor histidine ... 126 5e-30 gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase tra... 118 8e-28 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 952 bits (2462), Expect = 0.0, Method: Compositional matrix adjust. Identities = 471/475 (99%), Positives = 473/475 (99%) Query: 10 LSDRFFYRANIRLKLFYAMLALIVALIAAINGAGHYIFNRDFLGYLNEQAQQRMEDMVPR 69 +SDRFFYRANIRLKLFYAMLALIVALIAAINGAGHYIFNRDFLGYLNEQAQQRMEDMVPR Sbjct: 1 MSDRFFYRANIRLKLFYAMLALIVALIAAINGAGHYIFNRDFLGYLNEQAQQRMEDMVPR 60 Query: 70 LEQAYREHGSWDFIRDNPRAWFELMKPAIVEHNGATRSAGPTFTASDLTGATLRFSLLDA 129 LEQAYREHGSWDFIRDNPRAWFELMKPAIVEHNGA RSAGPTFTASDLTGATLRFSLLDA Sbjct: 61 LEQAYREHGSWDFIRDNPRAWFELMKPAIVEHNGAARSAGPTFTASDLTGATLRFSLLDA 120 Query: 130 SRRPVIGYPNVGPDARLRPLLQDDVIIGWVAQTPFESVIGALDLRFQQSQFAAQIIIAVV 189 SRRPVIGYPNVGPDARLRPLLQDDVIIGWVAQTPFESVIGALDLRFQQSQFAAQIIIAVV Sbjct: 121 SRRPVIGYPNVGPDARLRPLLQDDVIIGWVAQTPFESVIGALDLRFQQSQFAAQIIIAVV 180 Query: 190 AVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVESGDEVGMLAQDFNQLAMT 249 AVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVESGDEVGMLAQDFNQLAMT Sbjct: 181 AVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVESGDEVGMLAQDFNQLAMT 240 Query: 250 LERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLRSLKSEVEQLNKLVG 309 LERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLRSLKSEVE LNKLVG Sbjct: 241 LERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLRSLKSEVEHLNKLVG 300 Query: 310 DLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPASMPVHGDAAR 369 DLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPASMPVHGDAAR Sbjct: 301 DLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPASMPVHGDAAR 360 Query: 370 LQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFEDSEPGVPPEVMARLFERFFRVE 429 LQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVF+DSEPGVPPEVMARLFERFFRVE Sbjct: 361 LQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFDDSEPGVPPEVMARLFERFFRVE 420 Query: 430 ASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPARLEDERGSA 484 ASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPARLEDERGSA Sbjct: 421 ASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPARLEDERGSA 475 >gi|300311272|ref|YP_003775364.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 143 bits (361), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 106/300 (35%), Positives = 161/300 (53%), Gaps = 9/300 (3%) Query: 180 FAAQIIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRV----EVESGDE 235 F+ ++IA+ ++ A + +S L+R L VA A RLA+GD++ R +V + Sbjct: 99 FSLLVLIALPLIVGAGLM---LSLRLSRHLDAVAVAARRLAAGDFSARALSCKQVPAA-- 153 Query: 236 VGMLAQDFNQLAMTLERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLR 295 + L +D N++A LER E+ A ++HELRTPL +L+ + DG+ Q L Sbjct: 154 LDALMRDVNEMAERLERRERELHASSAAIAHELRTPLTAAKARLQGIMDGIFDRSPQQLS 213 Query: 296 SLKSEVEQLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSC 355 + +++QLN+L+ DLY LSL AG L R+ L PLL + A + M + Sbjct: 214 MIMRQLDQLNRLISDLYLLSLVSAGQLALVRSQFQLRPLLEERLAWAAPSLRLLPMSTAL 273 Query: 356 ELPASMPVHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFEDSEPGVPP 415 +P++ D RL Q +S L +N+LRY +G L + A L H+ ED PG P Sbjct: 274 YCCEHLPLNADRDRLGQAISILIDNALRYAASGKLLTISAGLHDGQIHIDVEDDGPGFAP 333 Query: 416 EVMARLFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPA 475 E + +RF+R E SR+R +GG+GLGLA+ I +AH G + P GG R+ + LPA Sbjct: 334 EHLELACDRFWRAEHSRSRHAGGSGLGLAVAVAICEAHGGKLSVHNRPAGGARMHLELPA 393 >gi|300313246|ref|YP_003777338.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 361 Score = 142 bits (357), Expect = 7e-35, Method: Compositional matrix adjust. Identities = 99/293 (33%), Positives = 145/293 (49%), Gaps = 4/293 (1%) Query: 184 IIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVESG--DEVGMLAQ 241 ++ ++ + +A ++ VR+S + PL VA + R+A GD + R E DE L Sbjct: 70 LVTTLIGLSIALWVAVRLSRRILTPLNSVATSLRRVAQGDLSARAAAERQPLDEAWQLIN 129 Query: 242 DFNQLAMTLERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLRSLKSEV 301 DFN +A LER ++ R + A ++HELRTP+ IL G+L+ L DGV QP+ SL ++V Sbjct: 130 DFNAMAERLERVQQERIFWNAAIAHELRTPVTILRGRLQGLSDGVFQPEPALFSSLLAQV 189 Query: 302 EQLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPASM 361 E L +L+ DL + LA++G L + + DL + ++G RL E Sbjct: 190 EGLGRLIEDLRIVGLAESGHLRVQLQETDLQADIMTVLKACEPALQEKGFRLLLETGVQR 249 Query: 362 PVHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVFEDSEPGVPPEVMARL 421 D R++Q L L EN L++ G L H L EDS PG+ + Sbjct: 250 S-RCDPVRIRQALLALLENVLQHASPGPLRISTRSLAGMH-SLCVEDSGPGIAASDAQMI 307 Query: 422 FERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLP 474 F+ F R + + G+GLGLA+ R I AH GT AS LGG V P Sbjct: 308 FDAFHRGARAGDAQQKGSGLGLAVVRTIASAHHGTADCRASELGGACFEVRWP 360 >gi|300311514|ref|YP_003775606.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 473 Score = 126 bits (316), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 92/296 (31%), Positives = 152/296 (51%), Gaps = 9/296 (3%) Query: 184 IIIAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVE---VESGDEVGMLA 240 I++A++A ++ A L ++ + P+ + A ++ A+G+ + +E DE+ L Sbjct: 168 IVVALLASLVFALL---LAWYFSHPIGHLKNAFNQAANGNLDLELEPVMSARNDELADLG 224 Query: 241 QDFNQLAMTLERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLRSLKSE 300 +DF+ + L + +R + DVSHELR+P+A L + TL ++ E Sbjct: 225 RDFDVMTAKLRTLMESQRRLLHDVSHELRSPIARLQVAIGLARVQDKDKLDLTLERIERE 284 Query: 301 VEQLNKLVGDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPAS 360 +++KLVG+L LS +A I + +L D S G + +L Sbjct: 285 SVRMDKLVGELLTLSRLEANVPGSMEEPIAIEAMLGDMINDASFEAEAIGKHVDDQLDCR 344 Query: 361 MPVHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQ--QHWHLVFEDSEPGVPPEVM 418 + V G+ L + + N+ N++++T G +Q+H L + + HL D PGVP E M Sbjct: 345 VQVKGNPELLYRAIENVLRNAIKHTAPGTTVQIHTQLDEINKLLHLSILDRGPGVPEEEM 404 Query: 419 ARLFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLP 474 +FE FFR +++ +SG GLGL+I RRIV+AHQG I A+ GGLRV + LP Sbjct: 405 NSIFELFFRSSRTKSNSSG-HGLGLSIARRIVEAHQGFIQAANRSNGGLRVDITLP 459 >gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 459 Score = 118 bits (296), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 88/250 (35%), Positives = 132/250 (52%), Gaps = 17/250 (6%) Query: 235 EVGMLAQDFNQLAMTLERNEKLRREFMADVSHELRTPLAILNGQLE---ALEDGVLQPDA 291 E+ + + NQ L +++ F+AD +H+LRTPLA+++ Q++ L+D A Sbjct: 215 ELRPIVEAINQYIFRLNAQVTVQKRFVADAAHQLRTPLAVIDSQIQFARQLDDPARV--A 272 Query: 292 QTLRSLKSEVEQLNKLVGDLYDLSLADAG-ALTYRRADIDL----APLLRDSAALYSDRF 346 Q L SL+ + L L LS A+A + + RA +DL A +L + + L + Sbjct: 273 QVLASLQEGSRSMTDLTNKLLLLSQAEASDSSAFPRAPVDLLAVSAMVLEELSGLALKKG 332 Query: 347 SQRGMRLSCELPASMPVHGDAARLQQLLSNLFENSLRYTDAGGRLQVHAHLQQQHWHLVF 406 G+ + E S V+G+ A+L +L NL +N+LRYT GG++ V ++ + Sbjct: 333 IDLGLETTLE---SAMVNGNEAQLMGMLMNLIDNALRYTPEGGKVTVGLEKKRSQMIITV 389 Query: 407 EDSEPGVPPEVMARLFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLG- 465 D+ PG+P EV R+FERF+R A G+GLGLAI R IV A QG+I A G Sbjct: 390 SDNGPGIPAEVRERVFERFYRNAAPDQP---GSGLGLAIVREIVQASQGSIRLDAPAQGS 446 Query: 466 GLRVVVVLPA 475 GL V LPA Sbjct: 447 GLVATVSLPA 456 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,391,289 Number of extensions: 56432 Number of successful extensions: 372 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 302 Number of HSP's successfully gapped: 42 Length of query: 484 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 395 Effective length of database: 1,198,455 Effective search space: 473389725 Effective search space used: 473389725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)