BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0320.p (428 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313894|ref|YP_003777986.1| RND family efflux transporter M... 835 0.0 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 386 e-108 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 357 e-100 gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [... 129 7e-31 gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbas... 128 1e-30 >gi|300313894|ref|YP_003777986.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 428 Score = 835 bits (2156), Expect = 0.0, Method: Compositional matrix adjust. Identities = 422/428 (98%), Positives = 424/428 (99%) Query: 1 MHPTASTPRRQGRTSSATARLALSLILVAVLTACSRSGDQPKPTAQAEVGYVVVKAQRQA 60 MHPTASTPRRQGRTSSATARLALSLILVAVL ACSRSGDQPKPTAQAEVGYVVVKAQRQA Sbjct: 1 MHPTASTPRRQGRTSSATARLALSLILVAVLAACSRSGDQPKPTAQAEVGYVVVKAQRQA 60 Query: 61 LTTELPGRTTAYLAADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARAS 120 LTTELPGRTTAYLAADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARAS Sbjct: 61 LTTELPGRTTAYLAADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARAS 120 Query: 121 LLKAEATLASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINL 180 LLKAEATLASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINL Sbjct: 121 LLKAEATLASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINL 180 Query: 181 QYTKILSPISGRIEKSSVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELA 240 QYTKILSPISGRIEKS+VTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELA Sbjct: 181 QYTKILSPISGRIEKSAVTPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELA 240 Query: 241 SGQIRKSGENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPG 300 SGQIRKSGENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPG Sbjct: 241 SGQIRKSGENAAQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPG 300 Query: 301 AYVRAVLEEGVDDHALLVPQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVS 360 AYVRAVLEEGVDDHALL PQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVS Sbjct: 301 AYVRAVLEEGVDDHALLAPQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVS 360 Query: 361 RGLKEGDKLIVDGLQKVRTGDAPRAIDITDSLQKDLAREAAAAAADPSLGGDAGKEGAAA 420 RGLKEGDKLIVDGLQKVRTGDAPRAIDITDSLQKDLAREAAAAAADPSLGGDAGK+ A A Sbjct: 361 RGLKEGDKLIVDGLQKVRTGDAPRAIDITDSLQKDLAREAAAAAADPSLGGDAGKDSAPA 420 Query: 421 SATAAQSK 428 SATAAQSK Sbjct: 421 SATAAQSK 428 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 386 bits (991), Expect = e-108, Method: Compositional matrix adjust. Identities = 207/358 (57%), Positives = 263/358 (73%), Gaps = 2/358 (0%) Query: 24 SLILVAVLTACSRSGDQPKPTAQAEV-GYVVVKAQRQALTTELPGRTTAYLAADIRPQVG 82 +L +++VL+AC + P A V G V V AQ L TELPGRT+ Y AD+RPQV Sbjct: 15 ALAILSVLSACGKPPGGAPPAAGTPVLGVVTVHAQPVELHTELPGRTSPYQIADVRPQVN 74 Query: 83 GIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSKLKAERYAELN 142 GII+ R F+EGS VKAGQ LYQI+PASYQAAYDS+ A+L KAEA++ +++LKA RY EL Sbjct: 75 GIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAALAKAEASVRTARLKASRYKELV 134 Query: 143 KIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKSSVTPGA 202 I AVS+QD DDA ++L + +ADV +A VQT+ INL Y ++ +PISGRI KSSVTPGA Sbjct: 135 AIKAVSQQDYDDAVASLGEAEADVGSARANVQTSRINLDYARLDAPISGRIGKSSVTPGA 194 Query: 203 LVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRKSGENAAQVKIVLEDGS 262 LVTA+QTTALTT+QQ+DPIYVDVTQS+ L L+ LA+GQ+ KSG N A+V++VLE+G Sbjct: 195 LVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLKEALANGQLEKSG-NGAKVRLVLENGR 253 Query: 263 TYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEEGVDDHALLVPQKA 322 TY + G L+F+ ++V+ TG ITLRA+ PNP LLPG YVRAVL+EGV LLVPQ+A Sbjct: 254 TYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQA 313 Query: 323 VSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVSRGLKEGDKLIVDGLQKVRTG 380 VSRD G A V+ A G V+QR + RA+G+ W VS GLKEGD+L+VDG Q+ G Sbjct: 314 VSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQWLVSSGLKEGDQLVVDGQQRASAG 371 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 357 bits (917), Expect = e-100, Method: Compositional matrix adjust. Identities = 185/362 (51%), Positives = 253/362 (69%), Gaps = 2/362 (0%) Query: 20 RLALSLILVAVLTACS-RSGDQPKPTAQAEVGYVVVKAQRQALTTELPGRTTAYLAADIR 78 RL +++ + A L AC + Q P +EV V + QR A+T ELPGR A+ A +R Sbjct: 9 RLTMTVAIAAALVACGQKPAAQGGPMPPSEVSVVTIAPQRVAITNELPGRVEAFRVAQVR 68 Query: 79 PQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSKLKAERY 138 +V GI+QKR+FKEGS VK GQ L++IDPA Y+A+ +SA+A+L +A+A L + L A+RY Sbjct: 69 ARVAGIVQKRVFKEGSEVKEGQVLFKIDPAQYKASLESAQATLARAQANLTQTTLTAQRY 128 Query: 139 AELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKSSV 198 L +++AVSKQ+ D+A + +Q +ADV KAAVQTA++NL Y + SPISGRI ++ V Sbjct: 129 KPLVEVNAVSKQEYDNAVAAQKQAEADVAAGKAAVQTASLNLGYATVTSPISGRIGRALV 188 Query: 199 TPGALVTASQTTALTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIRKSGENAAQVKIVL 258 T GALV T L VQQ+DP+YV+VTQS+ DLL+L++ +A+GQ++ G+N A V + L Sbjct: 189 TEGALVGQGDATQLALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDNKASVTLEL 248 Query: 259 EDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEEGVDDHALLV 318 E+G Y PGKL F+ +SVD TG ++LRA PNP +LLPG YVRA +E+ VD+ ALLV Sbjct: 249 ENGQAYPLPGKLLFSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLV 308 Query: 319 PQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVSRGLKEGDKLIVDGLQKVR 378 PQ+AV RD G +T +V+ A+ KV+ R V A GNNW V GLKEGD++IV+GLQKVR Sbjct: 309 PQQAVVRD-AGGSTVMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEGDRVIVEGLQKVR 367 Query: 379 TG 380 G Sbjct: 368 PG 369 >gi|300309658|ref|YP_003773750.1| HlyD-family secretion protein [Herbaspirillum seropedicae SmR1] Length = 399 Score = 129 bits (323), Expect = 7e-31, Method: Compositional matrix adjust. Identities = 102/318 (32%), Positives = 161/318 (50%), Gaps = 10/318 (3%) Query: 67 GRTTAYLAADIRPQVGGIIQKRLFKEGSTVKAGQPLYQIDPASYQAAYDSARASLLKAEA 126 GR A ++RP VGG + FK+G+ VK G L+ IDP Y+A A A L AEA Sbjct: 69 GRIEAVDRVEVRPLVGGTLTAVHFKDGALVKKGDVLFTIDPRPYEAEVARAEAQLAGAEA 128 Query: 127 TLASSKLKAERYAELNKIDAVSKQDNDDAQSTLRQNQADVEVQKAAVQTAAINLQYTKIL 186 + R L +A++K+D ++ Q+ R+ A+V +AA++ A +NL+YT+I Sbjct: 129 RAGYTASDLARGQRLLSDNAIAKRDFEEKQNASREASANVAAARAALRAARLNLEYTRIT 188 Query: 187 SPISGRIEKSSVTPGALVTASQTTA-LTTVQQIDPIYVDVTQSTVDLLRLRRELASGQIR 245 +P+SGRI ++ VT G +V A LTT+ + +Y L+ R Sbjct: 189 APVSGRISRAEVTVGNVVNPGAANAPLTTLVSVAKVYASFDVDEQSFLKY-----VNPAR 243 Query: 246 KSGENA-AQVKIVLEDGSTYERPGKLEFTGLSVDTGTGMITLRAVVPNPDGVLLPGAYVR 304 +G ++ + EDG Y R G++ +DT +G I +RA+ N + L+PG Y R Sbjct: 244 AAGATVPVELGLANEDG--YSRKGRVGSVDNRLDTASGTIRVRAIFDNANSQLVPGLYAR 301 Query: 305 AVLEEGVDDHALLVPQKAVSRDQTGAATALVLGADGKVQQRLVSVARAVGNNWWVSRGLK 364 L G A+L+ +KA+ DQ +V D R V V + +GL+ Sbjct: 302 VRLGGGEPHQAVLIDEKAIGTDQD-KRFVMVADKDNHANYRQVVVGAGHDGLVVIEKGLQ 360 Query: 365 EGDKLIVDGLQKVRTGDA 382 G++++V+GLQ++R GDA Sbjct: 361 PGERIVVNGLQRIRPGDA 378 >gi|300311797|ref|YP_003775889.1| membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 128 bits (321), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 113/358 (31%), Positives = 168/358 (46%), Gaps = 31/358 (8%) Query: 37 SGDQPKPTAQAEVGYVVVKAQRQALTTELPG--RTTAYLAADIRPQVGGIIQKRLFKEGS 94 + DQP+ A V + Q + L L G TA + ++ ++ G ++K F+EG Sbjct: 37 AADQPQAKAPPPVSVTLASVQERDLPQYLAGVGTVTANASVVVKTRIDGQLEKVGFQEGE 96 Query: 95 TVKAGQPLYQIDPASYQAAYDSARASLLKAEATLASSKLKAERYAELNKIDAVSKQDNDD 154 VK GQ L QID + QA A A K ATL +++L +RY L DA ++Q D Sbjct: 97 DVKKGQLLAQIDARALQAQLAQAEAQRAKDSATLMNARLDLQRYTTLRSQDAATQQQLDT 156 Query: 155 AQSTLRQNQADVEVQKAAVQTAAINLQYTKILSPISGRIEKSSVTPGALVTASQTTALTT 214 ++ + Q +A V+ +A V A + L YT I +PISGR+ V PG +V A+ L Sbjct: 157 QKALVAQLEAAVKTDEAQVAYAKVQLSYTTINAPISGRVGARLVDPGNIVHATDANGLVV 216 Query: 215 VQQIDPIYVDVTQSTVDLLRLRRELASGQIR--KSGENAAQVKIVLEDGSTYERPGKLEF 272 + QIDPI + + L E +G R ++ + QV D GKL Sbjct: 217 INQIDPITL--------VFTLPEEAVAGINRAQRAHQQPLQVSAYARDSQEVLATGKLVL 268 Query: 273 TGLSVDTGTGMITLRAVVPNPDGVLLPGAYVRAVLEEGVDDHALLVPQKAVSRDQTGAAT 332 ++T G + L+A NPD L PG YV A L+ +AL VP A+ R+Q G Sbjct: 269 LDNQINTTNGTVQLKARFDNPDHKLWPGQYVNARLQMRSHGNALTVPAAAIQRNQQG--- 325 Query: 333 ALVLGADGKVQQRL--VSVAR--------AVGNNWWVSRGLKEGDKLIVDGLQKVRTG 380 V D + Q R+ V V R A GN ++ G ++++DG K++ G Sbjct: 326 TYVYAIDQEDQARMQPVKVERIQDGIAVLAAGN------AVQAGQRVVIDGQYKLKPG 377 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.127 0.340 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,174,315 Number of extensions: 48275 Number of successful extensions: 218 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 12 Length of query: 428 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 340 Effective length of database: 1,203,190 Effective search space: 409084600 Effective search space used: 409084600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)