BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0318.p (497 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 978 0.0 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 516 e-147 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 419 e-118 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 347 1e-96 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 221 9e-59 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 978 bits (2528), Expect = 0.0, Method: Compositional matrix adjust. Identities = 491/497 (98%), Positives = 492/497 (98%) Query: 1 MMQPVQRKSTLWPRTAMLLALTAALAGCANLAPDYTQPAAPVPASWTSQAAAQPAVGRAG 60 MMQPVQRKSTLWPRTAMLLALTAALAGCANLAPDYTQPAAPVPASWTSQAAAQPAVG AG Sbjct: 1 MMQPVQRKSTLWPRTAMLLALTAALAGCANLAPDYTQPAAPVPASWTSQAAAQPAVGSAG 60 Query: 61 PAAGEIDADHLGWREFFLDPRLQQVIATALDNNRDLRVAALNIEKARAQYRITDADRYPS 120 PAAGEIDADHLGWREFFLDPRLQQVIA ALDNNRDLRVAALNIEKARAQYRITDA+RYPS Sbjct: 61 PAAGEIDADHLGWREFFLDPRLQQVIAAALDNNRDLRVAALNIEKARAQYRITDANRYPS 120 Query: 121 ISATGASSASRTPAELSTTGRAAVSRQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQV 180 ISATG SSASRTPAELSTTG AAVS QQSVTVGVSAYELDFFGRLKNLSDAALESYQYQV Sbjct: 121 ISATGTSSASRTPAELSTTGSAAVSHQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQV 180 Query: 181 ETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLA 240 ETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLA Sbjct: 181 ETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLA 240 Query: 241 SQEATVESARADVARYASQVEQDLNALRLLVGADIDAALLPAGLPDTASVLPATPAGLPS 300 SQEATVESARADVARYASQVEQDLNALRLLVGADIDAALLPAGLPDTASVLPATPAGLPS Sbjct: 241 SQEATVESARADVARYASQVEQDLNALRLLVGADIDAALLPAGLPDTASVLPATPAGLPS 300 Query: 301 QVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTF 360 QVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTF Sbjct: 301 QVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTF 360 Query: 361 SPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQ 420 SPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQ Sbjct: 361 SPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQ 420 Query: 421 GKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVL 480 GKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVL Sbjct: 421 GKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVL 480 Query: 481 GGGWSEQTQPMQPGGQP 497 GGGWSEQTQPMQPGGQP Sbjct: 481 GGGWSEQTQPMQPGGQP 497 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 516 bits (1330), Expect = e-147, Method: Compositional matrix adjust. Identities = 274/476 (57%), Positives = 340/476 (71%), Gaps = 9/476 (1%) Query: 17 MLLALTAALAGCANLAPDYTQPAAPVPASWTSQAAAQPAVGRAGPAAGEIDADHLGWREF 76 ML + A L G L PDY +P APV A + + A + P +I GWR+ Sbjct: 15 MLPVMLAMLLGGCTLMPDYHRPDAPVAAHFAGADSKNAAGASSAPVVADI-----GWRDV 69 Query: 77 FLDPRLQQVIATALDNNRDLRVAALNIEKARAQYRITDADRYPSISATGASSASRTPAEL 136 F DP LQQVIA AL+NNRDLRVA LNIEKARAQY + A +PSI A + +ASRTP +L Sbjct: 70 FTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTPGDL 129 Query: 137 STTGRAAVSRQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVAT 196 S+TG A SR + T+G SAYELD FGR+++L + AL+S+ E RRST ISL+AEVAT Sbjct: 130 SSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIAEVAT 189 Query: 197 AWLTLASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARY 256 AWLTLASD+D L+LAQDT SQS ++AL+QRS ++G+ S + L + +VESAR DV Y Sbjct: 190 AWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVDVESY 249 Query: 257 ASQVEQDLNALRLLVGADIDAALLPAGLPDT----ASVLPATPAGLPSQVLLRRPDVLAA 312 +QV+QD NAL LLVG D+ ALLP GLPDT AS L P GLPS +L RR D++ A Sbjct: 250 TAQVDQDRNALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPPGLPSDLLQRRADIVQA 309 Query: 313 EHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAG 372 E L++ANA+IGAARAAF+PSI+LT N GTAS L GLFK GSGSW+F P I+LPIF+ G Sbjct: 310 ERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQISLPIFDGG 369 Query: 373 SLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFR 432 + +A+LD A ++RDI VA YEKAIQTAFREV+DALA R TL ++L AQ AS++AFR Sbjct: 370 ANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQASDEAFR 429 Query: 433 LSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSEQT 488 LS ARY +GVDSYLT L +QRTLYSAQ+ LI +L+ +N +T YK LGGGW E+T Sbjct: 430 LSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGWVERT 485 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 419 bits (1076), Expect = e-118, Method: Compositional matrix adjust. Identities = 239/483 (49%), Positives = 314/483 (65%), Gaps = 18/483 (3%) Query: 12 WPRTAMLLALTAAL------AGCANLAPDYTQPAAPVPASWTSQAAAQPAVGRAGPAAGE 65 PR+ LL + AAL GC+ LAP Y +P APV +S+ + AA GRA E Sbjct: 6 MPRSPRLLHMCAALVMAGVLGGCS-LAPTYERPQAPVESSYPADAA-----GRA-----E 54 Query: 66 IDADHLGWREFFLDPRLQQVIATALDNNRDLRVAALNIEKARAQYRITDADRYPSISATG 125 + A +LGWR+FF D RLQQ+IA AL+NNRDLR A LNIE+ARAQY+I AD P+++ +G Sbjct: 55 LQAVNLGWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSG 114 Query: 126 ASSASRTPAELSTTGRAAVSRQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRS 185 ++ +RT A S TG+ +S V + V AYELDFFGR++NL DAAL SY E ++S Sbjct: 115 NAARTRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKS 174 Query: 186 THISLVAEVATAWLTLASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEAT 245 ISLVA+VA A+L S + LKLA +T S+ L+Q+ D+G + +D + Sbjct: 175 AQISLVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTL 234 Query: 246 VESARADVARYASQVEQDLNALRLLVGADIDAALLPAGLPDTASVLPATPAGLPSQVLLR 305 VESAR + Q Q NAL LLVG I L PA L ++ PAGLPS +L Sbjct: 235 VESARVAKTQLERQRAQAENALTLLVGKKI-GELPPAQLLSDEKIVTDIPAGLPSDLLTN 293 Query: 306 RPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSIN 365 RPD+ +AE +L +ANA+IGAARAAFFP ISLT N G+AS TLGGLF++GSG+W+F PS++ Sbjct: 294 RPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLS 353 Query: 366 LPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATA 425 LPIF+ G A+LD A++ ++ V YEKAIQTAFREVADAL R +LD+++ AQ A Sbjct: 354 LPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLA 413 Query: 426 ASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWS 485 A ++ +L+D R++NG+ S L L AQR L+SAQQ LI R +N I LYK LGGG + Sbjct: 414 AQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLN 473 Query: 486 EQT 488 E T Sbjct: 474 ENT 476 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 347 bits (890), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 216/458 (47%), Positives = 282/458 (61%), Gaps = 11/458 (2%) Query: 28 CANLAPDYTQPAAPVPASWTSQAAAQPAVGRAGPAAGEIDADHLGWREFFLDPRLQQVIA 87 C +LAP Y +PAAPV A + P A A E + L WR++F DP LQ +I Sbjct: 25 CQSLAPHYQRPAAPVAAVYPDG----PVNEAAHQVANEAASPPLPWRDYFTDPPLQALIT 80 Query: 88 TALDNNRDLRVAALNIEKARAQYRITDADRYPSISATGASSASRTPAELSTTGRAAVSRQ 147 AL+N+RDLR AAL +++A+A Y I AD +P+I A SRTPA+L+ T R V Sbjct: 81 QALENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSA 140 Query: 148 QSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRDL 207 +G+S++E+D +GRL++L DAALES + TR + +SL+A+VA AWL LA + Sbjct: 141 YQAGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDER 200 Query: 208 LKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNAL 267 L++A T AS+ +F + R ++G S ++L E + A+A A+ Q Q LNAL Sbjct: 201 LEIANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNAL 260 Query: 268 RLLVGADIDAALLPAGLPDT---ASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIG 324 LLVG+ + +LP G A P P GLP+ +L RRPDV AAEH L +ANA+IG Sbjct: 261 TLLVGSPV---VLPVGQARAGLDAVFAPLQP-GLPALLLTRRPDVAAAEHRLMAANANIG 316 Query: 325 AARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKIT 384 AARAAFFPSISLT N GTAS L GLF+ GS +WTFSPSI LPIF AG LR +L +++ Sbjct: 317 AARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVR 376 Query: 385 RDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDS 444 RD+ VA YEK +QTAFREVADALA R L Q+L+ A + RLS RY NG Sbjct: 377 RDLAVAAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAP 436 Query: 445 YLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGG 482 +L L AQR L SAQQ L+ +R S+ + LY LGG Sbjct: 437 FLDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 221 bits (564), Expect = 9e-59, Method: Compositional matrix adjust. Identities = 164/464 (35%), Positives = 239/464 (51%), Gaps = 11/464 (2%) Query: 22 TAALAGCANLAPDYTQPAAPVPASWTSQAAAQP-AVGRAGPAAGEIDADHLGWREFFLDP 80 T AL CA L AP+P S P + PA A +GW+++F Sbjct: 14 TLALCACAPLT-------APLPPSALDLPTHYPRTLAEDAPANAAPTASAIGWQDYFPQA 66 Query: 81 RLQQVIATALDNNRDLRVAALNIEKARAQYRITDADRYPSISATGASSASRTPAELSTTG 140 LQ +IA AL +N DLR L +++ +AQ+RI + P+++ + SR P E +G Sbjct: 67 PLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPESG 126 Query: 141 RAAVSRQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLT 200 AA +R+ V +G S++ELDF+GR+ +L++AAL+ + ++ ISL+ +VA AW + Sbjct: 127 PAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAWTS 186 Query: 201 LASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQV 260 L + L L + A+ + + AL Q HD G LA Q + + RA+ A Sbjct: 187 LRELDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAELQQMR 246 Query: 261 EQDLNALRLLVG-ADIDAALLPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSA 319 + L +L G + AAL PA P T + A GLPS +L RPD++AAEH L+++ Sbjct: 247 ASQRSTLEMLAGLTQLPAALEPA--PTTILLPFALAPGLPSTLLAARPDIIAAEHRLQAS 304 Query: 320 NADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLD 379 A + AARAAFFP ISLTA GG AS L LFK S +W F P I+LP+ + G ++ D Sbjct: 305 AAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYD 364 Query: 380 SAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYR 439 A + + + +YE+ +Q AFREVA L L ++L + + + R R Sbjct: 365 IAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLR 424 Query: 440 NGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGG 483 G S L L ++R AQQ L N+I LY LGGG Sbjct: 425 YGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGG 468 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.127 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,465,142 Number of extensions: 60636 Number of successful extensions: 441 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 385 Number of HSP's successfully gapped: 17 Length of query: 497 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 408 Effective length of database: 1,198,455 Effective search space: 488969640 Effective search space used: 488969640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)