BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0317.p (544 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313891|ref|YP_003777983.1| methyl-accepting chemotaxis I p... 1079 0.0 gi|300312783|ref|YP_003776875.1| methyl-accepting chemotaxis tra... 562 e-161 gi|300313014|ref|YP_003777106.1| methyl-accepting chemotaxis tra... 539 e-154 gi|300311037|ref|YP_003775129.1| methyl-accepting chemotaxis tra... 506 e-144 gi|300309796|ref|YP_003773888.1| methyl-accepting chemotaxis pro... 374 e-104 >gi|300313891|ref|YP_003777983.1| methyl-accepting chemotaxis I protein [Herbaspirillum seropedicae SmR1] Length = 544 Score = 1079 bits (2791), Expect = 0.0, Method: Compositional matrix adjust. Identities = 542/544 (99%), Positives = 542/544 (99%) Query: 1 MNQLSKLKIGTQLRLGFGIVIVLMLILITFALMRMNAISAAVQYQEDVTDTKLEPLYVAR 60 MNQLSKLKIGTQLRLGFGIVIVLMLILITFALMRMNAISAAVQYQE VTDTKLEPLYVAR Sbjct: 1 MNQLSKLKIGTQLRLGFGIVIVLMLILITFALMRMNAISAAVQYQETVTDTKLEPLYVAR 60 Query: 61 EALDQTGLAARNAYIFHDQAEALKELALLDQQKALYLDALRQLEPVLAGQAQFEKAKAGL 120 EALDQTGLAARNAYIFHDQAEALKELALLDQQKALYLDALRQLEPVLAGQAQFEKAKAGL Sbjct: 61 EALDQTGLAARNAYIFHDQAEALKELALLDQQKALYLDALRQLEPVLAGQAQFEKAKAGL 120 Query: 121 LAMAEELKRPRKYRESGQMEAYGEFLVKECSPLRRQIVQDIDALLKVVQRETKAAGDRAD 180 LAMAEELKRPRKYRESGQMEAYGEFLVKECSPLRRQIVQDIDALLKVVQRETKAAGDRAD Sbjct: 121 LAMAEELKRPRKYRESGQMEAYGEFLVKECSPLRRQIVQDIDALLKVVQRETKAAGDRAD 180 Query: 181 ALFASSLRLIMACAAIILGVSIATAWLIRKSLLGQLGGEPRYAVEITGRIAHGELSAPVD 240 ALFASSLRLIMACAAIILGVSIATAWLIRKSLLGQLGGEPRYAVEITGRIAHGELSAPVD Sbjct: 181 ALFASSLRLIMACAAIILGVSIATAWLIRKSLLGQLGGEPRYAVEITGRIAHGELSAPVD 240 Query: 241 TAPGDRSSLLYEIRVMRDKLASLVQQVRVGTHAIADASSEIAVGNQNLSERTEQQADTLE 300 TAPGDRSSLLYEIRVMRDKLASLVQQVRVGTHAIADASSEIAVGNQNLSERTEQQADTLE Sbjct: 241 TAPGDRSSLLYEIRVMRDKLASLVQQVRVGTHAIADASSEIAVGNQNLSERTEQQADTLE 300 Query: 301 KTVAAMDELTQAVRRNADNAQQAKHLAGSASDVASQAGTVVQQVVDTMGQIDASSRKIVD 360 KTVAAMDELTQAVRRNADNAQQAKHLAGSASDVASQAGTVVQQVVDTMGQIDASSRKIVD Sbjct: 301 KTVAAMDELTQAVRRNADNAQQAKHLAGSASDVASQAGTVVQQVVDTMGQIDASSRKIVD 360 Query: 361 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSASAAREIKSLIDS 420 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSASAAREIKSLIDS Sbjct: 361 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSASAAREIKSLIDS 420 Query: 421 SVSSVTDGSRLVEQAGATMQDVVESVQRVSAVVVEISAASQAQSDGILEVNTAIARLDEN 480 SVSSVTDGSRLVEQAGATMQDVVESVQRVSAVVVEISAASQAQSDGILEVNTAIARLDEN Sbjct: 421 SVSSVTDGSRLVEQAGATMQDVVESVQRVSAVVVEISAASQAQSDGILEVNTAIARLDEN 480 Query: 481 TQQNAALVEEAAAASQSMSQQADHLNQLVGAFKLSPAQMEGQHPAHLESQSIDVTPAPPR 540 TQQNAALVEEAAAASQSMSQQADHLNQLVGAFKLSPAQMEGQHPAHLESQSIDVTPAP R Sbjct: 481 TQQNAALVEEAAAASQSMSQQADHLNQLVGAFKLSPAQMEGQHPAHLESQSIDVTPAPAR 540 Query: 541 LGMR 544 LGMR Sbjct: 541 LGMR 544 >gi|300312783|ref|YP_003776875.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 542 Score = 562 bits (1448), Expect = e-161, Method: Compositional matrix adjust. Identities = 309/529 (58%), Positives = 384/529 (72%), Gaps = 6/529 (1%) Query: 4 LSKLKIGTQLRLGFGIVIVLMLILITFALMRMNAISAAVQYQEDVTDTKLEPLYVAREAL 63 +KIGT+L LGFG ++VL +++ +L + A++++++ V D KL PLY AREAL Sbjct: 2 FKHMKIGTKLALGFGALVVLTILVSGISLSSIRALTSSIKAVLAVQDQKLAPLYAAREAL 61 Query: 64 DQTGLAARNAYIFHDQAEALKELALLDQQKALYLDALRQLEPVLAGQAQFEKAKAGLLAM 123 DQTG+AARNAY+F A+A KELA++D+QK LYL A++ L P+ AG +FEK ++G+L M Sbjct: 62 DQTGIAARNAYVFRSDADAQKELAIVDEQKQLYLKAIKDLGPLFAGNKEFEKVRSGMLQM 121 Query: 124 AEELKRPRKYRESGQMEAYGEFLVKECSPLRRQIVQDIDALLKVVQRETKAAGDRADALF 183 AEELKRPR YRESG+ E Y +FLV ECSPLRRQIV DI +L V+ E A D D + Sbjct: 122 AEELKRPRLYRESGRSEEYRDFLVNECSPLRRQIVSDIGTVLAQVEAENNRAQDATDKMA 181 Query: 184 ASSLRLIMACAAIILGVSIATAWLIRKSLLGQLGGEPRYAVEITGRIAHGELSAPVDTAP 243 + + +++ +SI A L+ + LL QLGGEP YA +I +IA G+L+A V T Sbjct: 182 QLAALTVAVLTLVVVVISILIAVLLTRGLLKQLGGEPTYAAQIAQQIADGDLTANVQTKT 241 Query: 244 GDRSSLLYEIRVMRDKLASLVQQVRVGTHAIADASSEIAVGNQNLSERTEQQADTLEKTV 303 GD SSLL+ I+ MRD L S+V QVR GT I ASSEIA GN +LS RTEQQA +LE+T Sbjct: 242 GDNSSLLFAIKNMRDSLVSIVAQVRQGTFTIEQASSEIASGNLDLSSRTEQQASSLEETA 301 Query: 304 AAMDELTQAVRRNADNAQQAKHLAGSASDVASQAGTVVQQVVDTMGQIDASSRKIVDIIG 363 +AM+EL V++NADNA+QA LA SAS+VASQ G VV QVV TM I+ASSRKIVDII Sbjct: 302 SAMEELMTTVKQNADNAKQASQLAVSASEVASQGGEVVAQVVTTMDGINASSRKIVDIIS 361 Query: 364 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSASAAREIKSLIDSSVS 423 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA+AA+EI LI+ SVS Sbjct: 362 VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSATAAKEITELINDSVS 421 Query: 424 SVTDGSRLVEQAGATMQDVVESVQRVSAVVVEISAASQAQSDGILEVNTAIARLDENTQQ 483 + GS LVEQAG TM++VV SV+RV+ VV EIS ASQ QS GI +VNTAI +DE TQQ Sbjct: 422 QIERGSSLVEQAGQTMENVVNSVKRVTDVVAEISMASQEQSHGITQVNTAITHMDEATQQ 481 Query: 484 NAALVEEAAAASQSMSQQADHLNQLVGAFKLSPAQM------EGQHPAH 526 N+ALVEEAAAA+ SM +QA L+ LV F+L Q + PAH Sbjct: 482 NSALVEEAAAAANSMKEQAGALSGLVATFRLDAQQARQSGYSQASAPAH 530 >gi|300313014|ref|YP_003777106.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 553 Score = 539 bits (1388), Expect = e-154, Method: Compositional matrix adjust. Identities = 306/519 (58%), Positives = 383/519 (73%), Gaps = 2/519 (0%) Query: 1 MNQLSKLKIGTQLRLGFGIVIVLMLILITFALMRMNAISAAVQYQEDVTDTKLEPLYVAR 60 M+ +KIGT+L LGF +++ L +IL +L +N ++ +++ +DV +L PLY+AR Sbjct: 9 MSGFKNMKIGTKLSLGFALLLTLTIILAGISLFSINRLTGSIKAADDVQVERLLPLYLAR 68 Query: 61 EALDQTGLAARNAYIFHDQAEALKELALLDQQKALYLDALRQLEPVLAGQAQFEKAKAGL 120 EALDQTGLAARNAY+F +A+A KELA++D+QK +YL AL+ +EP G F+K + GL Sbjct: 69 EALDQTGLAARNAYVFRTEADAQKELAIVDEQKQIYLKALKDMEPHFVGHKGFDKVRTGL 128 Query: 121 LAMAEELKRPRKYRESGQMEAYGEFLVKECSPLRRQIVQDIDALLKVVQRETKAAGDRAD 180 L MAEELKRPR YRESG+ME + +FLV ECSPLRRQIV DI+ +++ + A Sbjct: 129 LQMAEELKRPRIYRESGKMEEFRDFLVNECSPLRRQIVLDINKVIEEADADNIKAHQTTV 188 Query: 181 ALFASSLRLIMACAAIILGVSIATAWLIRKSLLGQLGGEPRYAVEITGRIAHGELSAPVD 240 + A + L+ I+ +S A++I +SLL QLGGEP YA +I +IA G+L+A VD Sbjct: 189 DMAAMASSLVAVLTGIVAVISALVAFVITRSLLKQLGGEPAYAADIAQQIASGDLTADVD 248 Query: 241 TAPGDRSSLLYEIRVMRDKLASLVQQVRVGTHAIADASSEIAVGNQNLSERTEQQADTLE 300 PGD +SLL+ I+ MRD LA++V +VR GTHAI AS+EIA GN +LS RTEQQA LE Sbjct: 249 AKPGDTTSLLFAIKKMRDNLAAIVGKVREGTHAIEQASAEIATGNMDLSSRTEQQAGALE 308 Query: 301 KTVAAMDELTQAVRRNADNAQQAKHLAGSASDVASQAGTVVQQVVDTMGQIDASSRKIVD 360 +T +AM+EL V++NADNA+QA LA SASDVASQ G VV QVV TM I+ASSRKIVD Sbjct: 309 ETASAMEELMSTVKQNADNARQASQLAVSASDVASQGGQVVGQVVSTMEGINASSRKIVD 368 Query: 361 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSASAAREIKSLIDS 420 II VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA+AA+EI LI+ Sbjct: 369 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSATAAKEITDLIND 428 Query: 421 SVSSVTDGSRLVEQAGATMQDVVESVQRVSAVVVEISAASQAQSDGILEVNTAIARLDEN 480 SV + GS LV QAGATM++VV SV+RV+ VV EIS ASQ QS GI +VN AI +DE Sbjct: 429 SVGQIEQGSSLVGQAGATMENVVTSVKRVTDVVAEISHASQEQSHGIAQVNLAITHMDEA 488 Query: 481 TQQNAALVEEAAAASQSMSQQADHLNQLVGAFKL--SPA 517 TQQN+ALVEEAAAA+ SM QA+ L LV FKL SPA Sbjct: 489 TQQNSALVEEAAAAANSMKTQAEQLAGLVSTFKLAHSPA 527 >gi|300311037|ref|YP_003775129.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 522 Score = 506 bits (1302), Expect = e-144, Method: Compositional matrix adjust. Identities = 287/516 (55%), Positives = 382/516 (74%) Query: 1 MNQLSKLKIGTQLRLGFGIVIVLMLILITFALMRMNAISAAVQYQEDVTDTKLEPLYVAR 60 M LS++KIG +L LGF ++L+L L+ A++ +N++ + Y + + +LEPLY+AR Sbjct: 1 MKVLSEMKIGVKLSLGFASQLLLLLALVAAAMLGINSLRETIVYANKLKNEQLEPLYLAR 60 Query: 61 EALDQTGLAARNAYIFHDQAEALKELALLDQQKALYLDALRQLEPVLAGQAQFEKAKAGL 120 EALDQTG+AARNAY+F A+A EL ++D+QKA+YL L+ LEP LA +A F + GL Sbjct: 61 EALDQTGIAARNAYVFVSDADAKNELNIVDEQKAIYLQQLQLLEPRLADRAGFAAVREGL 120 Query: 121 LAMAEELKRPRKYRESGQMEAYGEFLVKECSPLRRQIVQDIDALLKVVQRETKAAGDRAD 180 L MAEELKRPR+YR+SG MEAY +FLV ECSPLRR+IV +I+ L+ + E+ AGD A+ Sbjct: 121 LQMAEELKRPRRYRDSGAMEAYRDFLVNECSPLRRKIVANINQLIDQISAESTTAGDTAE 180 Query: 181 ALFASSLRLIMACAAIILGVSIATAWLIRKSLLGQLGGEPRYAVEITGRIAHGELSAPVD 240 +L I A + L +++ A+ I +SLL QLGGEP YA+ I RIAHG+L+ V Sbjct: 181 NAAKQALLFISLLAGLALLIAVVIAFFITRSLLRQLGGEPAYAMGIAQRIAHGDLAVDVV 240 Query: 241 TAPGDRSSLLYEIRVMRDKLASLVQQVRVGTHAIADASSEIAVGNQNLSERTEQQADTLE 300 T D+SSLLY I++MRD L+ +V QVR GT +I A SEI+ GN +LS RTE+QA +LE Sbjct: 241 TRSHDQSSLLYAIKMMRDSLSRIVAQVRQGTDSIERACSEISSGNADLSGRTERQASSLE 300 Query: 301 KTVAAMDELTQAVRRNADNAQQAKHLAGSASDVASQAGTVVQQVVDTMGQIDASSRKIVD 360 +T +AM+++T AVR++ADNA QA LAGSAS +A + G ++ VV TM I SS KIVD Sbjct: 301 ETASAMEQITSAVRQSADNAIQANQLAGSASSIALKGGQMMGDVVATMEDIHHSSVKIVD 360 Query: 361 IIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSASAAREIKSLIDS 420 II VIDGIAFQTNILALNAAVEAARAGE GRGFAVVA+EVR+LAQRSA+AA+EIK LI + Sbjct: 361 IISVIDGIAFQTNILALNAAVEAARAGEGGRGFAVVASEVRTLAQRSAAAAKEIKELISA 420 Query: 421 SVSSVTDGSRLVEQAGATMQDVVESVQRVSAVVVEISAASQAQSDGILEVNTAIARLDEN 480 SV+ V GS LV+ AG TM ++V SVQ VS V+ EI+A+S+ QS GI +VN AI+ +DE Sbjct: 421 SVAKVESGSGLVKGAGETMGELVGSVQNVSKVIAEITASSREQSTGIEQVNDAISHMDEV 480 Query: 481 TQQNAALVEEAAAASQSMSQQADHLNQLVGAFKLSP 516 TQQN+ALVE++AAA+++++ QA L +LV FK++P Sbjct: 481 TQQNSALVEQSAAAAEALTAQAQSLARLVSTFKVAP 516 >gi|300309796|ref|YP_003773888.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 374 bits (959), Expect = e-104, Method: Compositional matrix adjust. Identities = 247/553 (44%), Positives = 358/553 (64%), Gaps = 19/553 (3%) Query: 5 SKLKIGTQLRLGFGIVIVLMLILITFALMRMNAISAAVQYQEDVTDTKLEPLYVAREALD 64 S +K+GT+L LGF V++L++++ F + M+ + + V + K + +E L+ Sbjct: 3 SGMKMGTRLGLGFAWVVLLLVLVTGFGIFNMHQVQGRFTH---VVEVKNPESALVKEMLE 59 Query: 65 QTG---LAARNAYIFHDQAEALKELALLDQQKALYLDALRQLEPVLAGQAQF-EKAKAGL 120 G ++ RN + + E ++ Q Y A ++L+ + A + ++ KA L Sbjct: 60 TVGERSISLRNLMLPAAPEDVDIEAQYIEAQSLKYKMAAQKLQQLFAESSDTSDEEKAAL 119 Query: 121 LAMAEELKRPRKY----RESGQM-EAY--GEFLVKECSPLRRQIVQDIDALLKVVQRETK 173 +A + + K E G+ EAY +FL ++ P+++ ++ AL + + K Sbjct: 120 PKIAAQASKAEKLINEAVEFGKRREAYELAQFLKEQYLPVQKSWQVELKALAALEDQLNK 179 Query: 174 AAGDRADALFASSLRLIMACAAIILGVSIATAWLIRKSLLGQLGGEPRYAVEITGRIAHG 233 A + + ++ + L++ +AI + ++ A+ + + LL +LGGEP YAV+I G+IA G Sbjct: 180 EAAEASAVAYSRARMLMLLISAIGVVTAVLAAFWLTRHLLRKLGGEPDYAVQIAGQIAEG 239 Query: 234 ELSAPVDTAPGDRSSLLYEIRVMRDKLASLVQQVRVGTHAIADASSEIAVGNQNLSERTE 293 +L+ +DT GD SL+ +RVMRDKLA++V +VR G+ I ASSEIA GN +LS RTE Sbjct: 240 DLAVVIDTRAGDHDSLMAAMRVMRDKLAAIVAEVRKGSETITTASSEIARGNLDLSGRTE 299 Query: 294 QQADTLEKTVAAMDELTQAVRRNADNAQQAKHLAGSASDVASQAGTVVQQVVDTMGQIDA 353 QQA LE+T ++M++L V++NA+NA QA LA +ASDVA Q G++V QVV TM I+A Sbjct: 300 QQAGALEETASSMEQLNSTVKQNAENAHQANDLALAASDVAVQGGSIVSQVVQTMQSINA 359 Query: 354 SSRKIVDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRSLAQRSASAARE 413 SS+K+ DI+ VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSASAA+E Sbjct: 360 SSQKVADIVSVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKE 419 Query: 414 IKSLIDSSVSSVTDGSRLVEQAGATMQDVVESVQRVSAVVVEISAASQAQSDGILEVNTA 473 IK LI SV V +GS+LVEQAG+TM D+V S++RV+ V+ EI+ A++ QS GI +VN A Sbjct: 420 IKVLIGDSVGKVENGSKLVEQAGSTMHDIVTSIRRVTDVMTEINHANREQSSGIEQVNEA 479 Query: 474 IARLDENTQQNAALVEEAAAASQSMSQQADHLNQLVGAFKLSPAQMEGQHPAHLESQS-- 531 I ++D+ TQQNAALVE+AAAA++S+ +QA L QLV F+L AQ G A + + Sbjct: 480 IIQIDDMTQQNAALVEQAAAAARSLQEQAAGLQQLVSVFRLDAAQAHGHTAAPAHAPAAV 539 Query: 532 ---IDVTPAPPRL 541 IDVTP P L Sbjct: 540 ERVIDVTPGAPEL 552 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.128 0.335 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,230,066 Number of extensions: 43897 Number of successful extensions: 476 Number of sequences better than 1.0e-08: 69 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 71 Length of query: 544 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 454 Effective length of database: 1,193,720 Effective search space: 541948880 Effective search space used: 541948880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)