BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0314.p (277 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313889|ref|YP_003777981.1| amino acid ABC transporter perm... 553 e-159 gi|300313166|ref|YP_003777258.1| amino acid ABC transporter perm... 236 2e-63 gi|300309585|ref|YP_003773677.1| amino acid ABC transporter perm... 230 1e-61 gi|300313536|ref|YP_003777628.1| amino acid ABC transporter perm... 204 8e-54 gi|300310827|ref|YP_003774919.1| amino acid ABC transporter perm... 197 9e-52 >gi|300313889|ref|YP_003777981.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 277 Score = 553 bits (1426), Expect = e-159, Method: Compositional matrix adjust. Identities = 277/277 (100%), Positives = 277/277 (100%) Query: 1 MNFSLDFSPLAPYWPVFLHGAWLTLKMTTLAVIVGVAIGIFVAFAKNSPHRWLARGCSAY 60 MNFSLDFSPLAPYWPVFLHGAWLTLKMTTLAVIVGVAIGIFVAFAKNSPHRWLARGCSAY Sbjct: 1 MNFSLDFSPLAPYWPVFLHGAWLTLKMTTLAVIVGVAIGIFVAFAKNSPHRWLARGCSAY 60 Query: 61 IEVVRNTPFLVQIFLLYFGLASLGIRMPTFAAAVLAMIINIAAYAAEIIRAGLDSVPRGQ 120 IEVVRNTPFLVQIFLLYFGLASLGIRMPTFAAAVLAMIINIAAYAAEIIRAGLDSVPRGQ Sbjct: 61 IEVVRNTPFLVQIFLLYFGLASLGIRMPTFAAAVLAMIINIAAYAAEIIRAGLDSVPRGQ 120 Query: 121 IEAAQCLGLSVWRIRWHVMLQPAIERVYPALTSQFLLMMQASAMASQISAEELTAIANTV 180 IEAAQCLGLSVWRIRWHVMLQPAIERVYPALTSQFLLMMQASAMASQISAEELTAIANTV Sbjct: 121 IEAAQCLGLSVWRIRWHVMLQPAIERVYPALTSQFLLMMQASAMASQISAEELTAIANTV 180 Query: 181 QSDTFRSLETYLVVAALYLVLAILVKLVAYAIGETIFKRRRTLRRAAAQGRRTPVSRLPA 240 QSDTFRSLETYLVVAALYLVLAILVKLVAYAIGETIFKRRRTLRRAAAQGRRTPVSRLPA Sbjct: 181 QSDTFRSLETYLVVAALYLVLAILVKLVAYAIGETIFKRRRTLRRAAAQGRRTPVSRLPA 240 Query: 241 TDPAAMAPLAHVPYVHPASRPVAVNTAASLRIEGSAS 277 TDPAAMAPLAHVPYVHPASRPVAVNTAASLRIEGSAS Sbjct: 241 TDPAAMAPLAHVPYVHPASRPVAVNTAASLRIEGSAS 277 >gi|300313166|ref|YP_003777258.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 227 Score = 236 bits (601), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 116/224 (51%), Positives = 163/224 (72%), Gaps = 2/224 (0%) Query: 1 MNFSLDFSPLAPYWPVFLHGAWLTLKMTTLAVIVGVAIGIFVAFAKNSPHRWLARGCS-A 59 MN++ DF + W L GAWLT++++ L++++G A+G A A S L RG A Sbjct: 1 MNYTFDFMSVFANWDRLLMGAWLTIQLSALSIVLGFAVGTLCAIAGKSRLA-LVRGLVLA 59 Query: 60 YIEVVRNTPFLVQIFLLYFGLASLGIRMPTFAAAVLAMIINIAAYAAEIIRAGLDSVPRG 119 Y+E++RNTP LVQ+FL++FGLAS+G ++ AAV+A+ +N+ AY EI+RAG++S+ G Sbjct: 60 YVEIIRNTPLLVQVFLVFFGLASIGWKLSAETAAVIALTVNVGAYTTEIMRAGINSIHPG 119 Query: 120 QIEAAQCLGLSVWRIRWHVMLQPAIERVYPALTSQFLLMMQASAMASQISAEELTAIANT 179 QIEAA+CLG+S + + WHV+L PA+ERVYP+LTSQF+L+M AS++ SQISAEELTA AN Sbjct: 120 QIEAAECLGMSRFHVYWHVVLLPAVERVYPSLTSQFILLMLASSITSQISAEELTATANL 179 Query: 180 VQSDTFRSLETYLVVAALYLVLAILVKLVAYAIGETIFKRRRTL 223 VQS+TFRS E Y V+A YLVL+ L + +AI + F R+R L Sbjct: 180 VQSETFRSFEVYAVIAVAYLVLSFLFRGAFWAISQIAFVRKRKL 223 >gi|300309585|ref|YP_003773677.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 227 Score = 230 bits (586), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 113/223 (50%), Positives = 162/223 (72%) Query: 1 MNFSLDFSPLAPYWPVFLHGAWLTLKMTTLAVIVGVAIGIFVAFAKNSPHRWLARGCSAY 60 M++ DF+ +A +WP+ L GAW T++++ LA ++G +G A A+ S H WL S Y Sbjct: 1 MHYEFDFASVAAHWPLLLQGAWTTVQLSFLATLLGFVLGAACAVARGSRHGWLRAAVSGY 60 Query: 61 IEVVRNTPFLVQIFLLYFGLASLGIRMPTFAAAVLAMIINIAAYAAEIIRAGLDSVPRGQ 120 +E++RNTP L+Q + L FGL+S+G+ MP A A LA++INI AY+ EI+RAGL+S+ GQ Sbjct: 61 VELIRNTPLLIQCYFLIFGLSSVGVTMPIMAGATLALVINIGAYSCEIVRAGLESIHHGQ 120 Query: 121 IEAAQCLGLSVWRIRWHVMLQPAIERVYPALTSQFLLMMQASAMASQISAEELTAIANTV 180 +EAA CLGLS ++ WHV+L PA+ERV+PALTSQF+LMM AS++ S + AEEL +AN V Sbjct: 121 LEAASCLGLSPAQVFWHVVLLPAVERVWPALTSQFVLMMLASSILSSVGAEELLGVANRV 180 Query: 181 QSDTFRSLETYLVVAALYLVLAILVKLVAYAIGETIFKRRRTL 223 QSDTFR+ E +L++ A YL LA L++ + +G+ +F RRR L Sbjct: 181 QSDTFRNFEVFLLLWAAYLALACLMRAGFWLLGQLVFTRRRKL 223 >gi|300313536|ref|YP_003777628.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 223 Score = 204 bits (518), Expect = 8e-54, Method: Compositional matrix adjust. Identities = 101/222 (45%), Positives = 150/222 (67%) Query: 1 MNFSLDFSPLAPYWPVFLHGAWLTLKMTTLAVIVGVAIGIFVAFAKNSPHRWLARGCSAY 60 M + DF Y V + G T+++ + ++GVA+GIF A+A+ WL S Y Sbjct: 1 MAYQFDFLSTFDYTDVIVKGVATTIELIAIGGVLGVAVGIFGAWARTQGPGWLKPVVSGY 60 Query: 61 IEVVRNTPFLVQIFLLYFGLASLGIRMPTFAAAVLAMIINIAAYAAEIIRAGLDSVPRGQ 120 +E++RNTPFLVQ+F ++FGL SL I + AA++AM+IN+ AY+ EIIRAG+ ++PRGQ Sbjct: 61 VEIIRNTPFLVQLFFIFFGLPSLDIHISEITAAIVAMVINLGAYSTEIIRAGVQAIPRGQ 120 Query: 121 IEAAQCLGLSVWRIRWHVMLQPAIERVYPALTSQFLLMMQASAMASQISAEELTAIANTV 180 IEAAQ L +S +I H++L+PA+++++PAL+SQ +++M SA+ SQI+ EEL+ AN + Sbjct: 121 IEAAQSLSMSRMQIFRHIILRPALQKIWPALSSQVVIVMLGSAVCSQIAVEELSFAANFI 180 Query: 181 QSDTFRSLETYLVVAALYLVLAILVKLVAYAIGETIFKRRRT 222 Q FR+ E Y+V A+YLVLAIL++ V IG RRT Sbjct: 181 QGRNFRAFEAYIVATAIYLVLAILLRQVLRLIGHYFITARRT 222 >gi|300310827|ref|YP_003774919.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 231 Score = 197 bits (500), Expect = 9e-52, Method: Compositional matrix adjust. Identities = 107/235 (45%), Positives = 164/235 (69%), Gaps = 8/235 (3%) Query: 1 MNFSLDFSPLAPYWPVFLHGAWLTLKMTTLAVIVGVAIGIF---VAFAKNSPHRWLARGC 57 M + F+ + + L GA LT++++ L++I G+A+GIF ++ + P R Sbjct: 1 MQYDFQFAFIFQHLDQLLVGALLTIRLSALSMICGLAVGIFGAIISIHGSKPARL---AV 57 Query: 58 SAYIEVVRNTPFLVQIFLLYFGLASLGIRMPTFAAAVLAMIINIAAYAAEIIRAGLDSVP 117 ++Y+E++R+TPFLVQ+F+++FGL S+G+++ AA++AM +N+ AY+ EI+RAG+ ++ Sbjct: 58 TSYVELIRSTPFLVQLFIIFFGLPSIGVQVDANIAALVAMTVNLGAYSTEIVRAGMMAIH 117 Query: 118 RGQIEAAQCLGLSVWRIRWHVMLQPAIERVYPALTSQFLLMMQASAMASQISAEELTAIA 177 QIEAAQ L ++ W++ HV+L PA+E+VYPAL SQF LMM AS++ S ISAEELTA A Sbjct: 118 PSQIEAAQALAMTRWQVIRHVVLTPALEKVYPALASQFTLMMLASSIVSAISAEELTATA 177 Query: 178 NTVQSDTFRSLETYLVVAALYLVLAILVKLVAYAIGETIFKRRRTLRRAAAQGRR 232 N + ++TFRS E YLVV LY++LA+L +L + IG +FKRRR R A+ RR Sbjct: 178 NLLDAETFRSFEIYLVVMVLYILLALLFRLAFWLIGLVVFKRRR--RLGVARPRR 230 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 644,512 Number of extensions: 19344 Number of successful extensions: 201 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 191 Number of HSP's successfully gapped: 21 Length of query: 277 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 193 Effective length of database: 1,222,130 Effective search space: 235871090 Effective search space used: 235871090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)