BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0308.p (408 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 820 0.0 gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 446 e-126 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 164 1e-41 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 123 3e-29 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 95 1e-20 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 820 bits (2119), Expect = 0.0, Method: Compositional matrix adjust. Identities = 407/408 (99%), Positives = 408/408 (100%) Query: 1 MVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYILD 60 MVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYILD Sbjct: 11 MVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYILD 70 Query: 61 AVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKASTEWFPAK 120 AVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKASTEWFPAK Sbjct: 71 AVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKASTEWFPAK 130 Query: 121 ERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWMLFYKSPRD 180 ERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWMLFYKSPRD Sbjct: 131 ERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWMLFYKSPRD 190 Query: 181 QKLLSPEERAYILEGQEKAPEKVQRESWTKIVRSRNFWSIAIPRFLSEPAWQTFNAWIPL 240 QKLLSPEERAYILEGQEK+PEKVQRESWTKIVRSRNFWSIAIPRFLSEPAWQTFNAWIPL Sbjct: 191 QKLLSPEERAYILEGQEKSPEKVQRESWTKIVRSRNFWSIAIPRFLSEPAWQTFNAWIPL 250 Query: 241 YMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSRKLVMLVGSLS 300 YMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSRKLVMLVGSLS Sbjct: 251 YMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSRKLVMLVGSLS 310 Query: 301 MIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSGYL 360 MIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSGYL Sbjct: 311 MIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSGYL 370 Query: 361 GATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIARPRPEAIA 408 GATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIARPRPEAIA Sbjct: 371 GATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIARPRPEAIA 418 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 446 bits (1146), Expect = e-126, Method: Compositional matrix adjust. Identities = 216/402 (53%), Positives = 285/402 (70%), Gaps = 1/402 (0%) Query: 1 MVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYILD 60 ++ LV L I NYLARN+L V APT+ EL MSTQQYSY+V A+Q+ Y +MQPV G+I+D Sbjct: 11 IIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIMQPVCGFIVD 70 Query: 61 AVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKASTEWFPAK 120 +G +IGF +F + WS+ A ATGW SLA FRAL+G+TEAA IP G+KA EWFP K Sbjct: 71 LIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGMKAVAEWFPDK 130 Query: 121 ERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWMLFYKSPRD 180 E+SVA+GWFN G+S+GA+ APPLV++ + GW+ +FVV G LG +W LW FY+SPRD Sbjct: 131 EKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAALWFAFYRSPRD 190 Query: 181 QKLLSPEERAYILEGQEKAPEKVQRESWTK-IVRSRNFWSIAIPRFLSEPAWQTFNAWIP 239 LS ER I+ GQ + V+ + K +VRSR FW+IA+ RF +EPAWQTF+ WIP Sbjct: 191 HAALSDSERETIISGQLRPVSAVRGKRPVKDVVRSRRFWAIALARFFAEPAWQTFSFWIP 250 Query: 240 LYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSRKLVMLVGSL 299 LY ERH+++K IAMFAWLPFLAAD+G + GGYLSP ++ L SR +++G++ Sbjct: 251 LYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWSRVCGVVLGAV 310 Query: 300 SMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGMSGY 359 MIGPACVG V SPYVAIAL +GGFAHQ +SG + ++++DVF +V TA+G GMS + Sbjct: 311 MMIGPACVGLVASPYVAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGTASGFAGMSAW 370 Query: 360 LGATLFTLLFGILVTQIGYGPLFVLLAAFDLVAVAVVFAIAR 401 +G F+LL G L +GYGPLF +L AFDL+ ++ + R Sbjct: 371 IGGLGFSLLVGALADTVGYGPLFGMLGAFDLIGAVLLIILIR 412 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 164 bits (416), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 111/380 (29%), Positives = 184/380 (48%), Gaps = 10/380 (2%) Query: 2 VTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYILDA 61 ++ + LA IVNYL R+TLS+A ++ +EL++S Q ++ A+ + YA Q G +LD Sbjct: 19 LSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPIGAMLDK 78 Query: 62 VGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKASTEWFPAKE 121 G ++ G WS+ A F Q R LG+ EA P G K +EWF +E Sbjct: 79 FGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPAGAKVVSEWFAMRE 138 Query: 122 RSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWMLFYKSPRDQ 181 R G F S+IG APP++ +L GW+M FV++G LG+ V W LFY++ R++ Sbjct: 139 RGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYLFYRN-RNE 197 Query: 182 KLLSPEERAYILEGQEKAPEKVQRESWTK---IVRSRNFWSIAIPRFLSEPAWQTFNAWI 238 L+P E Y+ + + P + SW++ + R W + + W+ Sbjct: 198 VQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYMVWLYLTWL 257 Query: 239 PLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSRK---LVML 295 P Y+ ERH++I +P++ +G + GY++ K + SRK L Sbjct: 258 PAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMPAIRSRKWPICAGL 316 Query: 296 VGSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTG 355 +G+ + PA + S +AI +S+ SG +++ S + VA+ + Sbjct: 317 IGAGAFTIPAA--YTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRRLVASLGSMQN 374 Query: 356 MSGYLGATLFTLLFGILVTQ 375 GYLG +L ++ G++V Q Sbjct: 375 FGGYLGGSLAPVITGVVVDQ 394 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 123 bits (308), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 101/384 (26%), Positives = 169/384 (44%), Gaps = 21/384 (5%) Query: 11 VNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYILDAVGTKIGFGI 70 VNY R TLS+A M EL + YI A+ Y + Q G++LD G+K + Sbjct: 25 VNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPGGWLLDRFGSKKIYMW 84 Query: 71 FALAWSLVCAAAAFATGWQSLA-------FFRALLGITEAAGIPGGVKASTEWFPAKERS 123 WS+ A T W S A R ++G+ EA P + WFP KER Sbjct: 85 SIALWSVFTFLQAGVT-WMSTAAAVASLFLLRFMVGLAEAPSFPANGRIVAAWFPTKERG 143 Query: 124 VAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVLWMLFYKSPRDQKL 183 A FN A+ PL+ W + W F V+G +G+ ++W SP+D Sbjct: 144 TASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIALALIWGRIIYSPKDHPR 203 Query: 184 LSPEERAYILEG---------QEKAPEKVQRESWTKIVRSRNFWSIAIPRFLSEPAWQTF 234 ++ E A+I G + AP R +++ +R + I ++ F Sbjct: 204 INQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRMLLGVYIGQYCINVITYFF 263 Query: 235 NAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSRKLVM 294 W P+Y+ ER M+I + A +P + IG V+GG +S K +S+ +RK+ + Sbjct: 264 LTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDRLIKR-GLSVTWARKIPI 322 Query: 295 LVGSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGAL-YSITSDVFTKNQVATATGL 353 + G L + +V++ ++ + ++++ F GAL +++ +D K V + GL Sbjct: 323 VGGMLLSMTMILCNYVEAQWMVVGIMALAFFGKGV--GALGWAVVADTAPKQIVGLSGGL 380 Query: 354 TGMSGYLGATLFTLLFGILVTQIG 377 M G + ++ G +V + G Sbjct: 381 FNMFGNVAGITTPIVIGYIVKETG 404 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 94.7 bits (234), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 98/412 (23%), Positives = 165/412 (40%), Gaps = 26/412 (6%) Query: 9 LIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAVMQPVAGYILDAVGTKIGF 68 I+NY+ R + + +L + + + + YA+ + + +L G + Sbjct: 33 FIINYIDRVNIGFVRSHLSTDLGIGAAAFGLGAGLFFVGYALFEVPSNMLLQRYGARAWL 92 Query: 69 GIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGVKASTEWFPAKERSVAIGW 128 W LV A AF S F R LLG+ EA PG V T+W P ER A+ Sbjct: 93 TRIMFTWGLVAACMAFVQSETSFYFMRFLLGVAEAGFFPGVVYYFTQWLPNHERGRAMAV 152 Query: 129 FNIGSSIGALCAPP------LVVWTILHGGWKMSFVVVGALGVIW-FVLWMLFYKSPRDQ 181 F GS+I ++ + P L+ LH GW+ F++ G +++ V+W++ +PR+ Sbjct: 153 FLSGSAIASILSGPITGALLLIEGGGLH-GWQWMFIIEGGASMVFCVVVWLVLDSTPREA 211 Query: 182 KLLSPEERAYI-----LEGQEKAPEKVQRESWTKIVRSRNFWSIAIPRFLSEPAWQTFNA 236 K LS E+R + E +++A + S ++R F + Sbjct: 212 KWLSQEQREALAAVIAAEQEQRAANRPAHVSALSLLRDPQIVLFCFIYFAIQLTIYGATF 271 Query: 237 WIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTSRKLVMLV 296 W+P + H N ++ +F +P++ A L L+ ++ K + + LV+ Sbjct: 272 WLPTIIKKMGHFNDFQVGLFNSIPWIVAIAAMYLFAVLAA---RYKKPQFWVATTLVISA 328 Query: 297 GSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVATATGLTGM 356 ++ + V P + + G + S + I A L Sbjct: 329 AGMA------ISAVAGPVIGFVAVCFAGIGFKAASSLFWPIPQAYLDARIAAAVLALINS 382 Query: 357 SGYLGATLFTLLFGILVTQIG--YGPLFVLLAAFDLVAVAVVFAIARPRPEA 406 G LG FG L + G G LF L A L+A +VF +AR RP A Sbjct: 383 LGNLGGFFAPATFGYLEQKTGSIQGGLFALSGA-SLLAAVLVF-LARTRPAA 432 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.138 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,213,817 Number of extensions: 47958 Number of successful extensions: 271 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 20 Length of query: 408 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 321 Effective length of database: 1,207,925 Effective search space: 387743925 Effective search space used: 387743925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)