BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0307.p
         (225 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313883|ref|YP_003777975.1| glutathione S-transferase [Herb...   451   e-128
gi|300313568|ref|YP_003777660.1| glutathione S-transferase [Herb...    62   4e-11
gi|300309973|ref|YP_003774065.1| glutathione S-transferase [Herb...    57   2e-09

>gi|300313883|ref|YP_003777975.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 225

 Score =  451 bits (1160), Expect = e-128,   Method: Compositional matrix adjust.
 Identities = 223/225 (99%), Positives = 224/225 (99%)

Query: 1   MQSSPTLLSHPVPTTPEDDVLQLYVDAQFASPYAMSVFVALREKQLPFTLHTLDLDRGQQ 60
           MQSSPTLLSHPVPTTPEDDVLQLYVDAQFASPYAMSVFVALREKQLPFTLHTLDLDRGQQ
Sbjct: 1   MQSSPTLLSHPVPTTPEDDVLQLYVDAQFASPYAMSVFVALREKQLPFTLHTLDLDRGQQ 60

Query: 61  HEPDYAQRSLTSRVPMLRHGALTLSESSAITEYLDEVFAGPRLYPQDAVARARARQVQAW 120
           HEPDYAQRSLTSRVPMLRHGALTLSESSAITEYLDEVFAGPRLYPQDAVARARARQVQAW
Sbjct: 61  HEPDYAQRSLTSRVPMLRHGALTLSESSAITEYLDEVFAGPRLYPQDAVARARARQVQAW 120

Query: 121 LRSDLMALREERNTQVVFYGAKKAALSEQASAAAQRLFHFAGQLLPAGADSLFGQWCIAD 180
           LRSDLMALREERNTQVVFYGAKKAALSE+ASAAAQRLFHFAGQLLP GADSLFGQWCIAD
Sbjct: 121 LRSDLMALREERNTQVVFYGAKKAALSEEASAAAQRLFHFAGQLLPVGADSLFGQWCIAD 180

Query: 181 VDLALMLNRLALHGDRVPPHLEDYARRQWQRPSVQAWLTQPRPAL 225
           VDLALMLNRLALHGDRVPPHLEDYARRQWQRPSVQAWLTQPRPAL
Sbjct: 181 VDLALMLNRLALHGDRVPPHLEDYARRQWQRPSVQAWLTQPRPAL 225


>gi|300313568|ref|YP_003777660.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 233

 Score = 62.0 bits (149), Expect = 4e-11,   Method: Compositional matrix adjust.
 Identities = 56/194 (28%), Positives = 91/194 (46%), Gaps = 8/194 (4%)

Query: 31  SPYAMSVFVALREKQLPFTLHTLDLDRGQQHEPDYAQRSLTSRVPMLRHGALTLSESSAI 90
           S ++M  +VA+    +PF    L LD+      + A+ + + RVP+L  G + + +S +I
Sbjct: 32  SSWSMRPWVAMVAFDIPFKEVRLLLDQADT-AAEIARYTGSGRVPVLLDGDIAIWDSLSI 90

Query: 91  TEYLDEVFAGPRLYPQDAVARARARQVQAWLRSDLMALREERNTQV--VFYGAKKAALSE 148
            EYL E F    L+P    ARA AR + A + S    LR   +  +   + G  + A   
Sbjct: 91  CEYLAEQFPDRHLWPARTDARAIARSICAEMHSGFTGLRSAMSMDIRGRYPGQGRTA--- 147

Query: 149 QASAAAQRLFHFAGQLLP--AGADSLFGQWCIADVDLALMLNRLALHGDRVPPHLEDYAR 206
           +A A   R+     + L      D LFG + IAD   A ++ R   +   + P L+ YA 
Sbjct: 148 EAQADIARVCEIWEECLSQFGHHDFLFGDFSIADAFYAPVVMRFISYQVPLAPALQAYAD 207

Query: 207 RQWQRPSVQAWLTQ 220
           R  + P+V  W+ +
Sbjct: 208 RVREHPAVARWIAE 221


>gi|300309973|ref|YP_003774065.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 218

 Score = 56.6 bits (135), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 47/153 (30%), Positives = 70/153 (45%), Gaps = 7/153 (4%)

Query: 74  VPMLRHGALTLSESSAITEYLDEVFAGPRLYPQDAVARARARQVQAWLRSDLMALREE-- 131
           VP L HG + + ++ AI EYL+E+    +L P D  ARA  R V   + S   ALR    
Sbjct: 56  VPSLHHGDVLVWDTLAIAEYLNEIRPAAKLLPADLAARAHCRSVCGEMHSGFSALRAALP 115

Query: 132 RNTQVVFYGAKKAALSEQASAAAQRLFHFAGQLLPA-GADSLFG-QWCIADVDLALMLNR 189
            N +  F   K   +  +A A   R+     + L   G   LFG Q  +AD   A ++ R
Sbjct: 116 MNLKGHFPNFK---VWSRAEADIVRITTIWKECLAKYGGPFLFGEQRTVADAMYAPVVTR 172

Query: 190 LALHGDRVPPHLEDYARRQWQRPSVQAWLTQPR 222
              +  ++ P    YA+R    P +Q W+T  +
Sbjct: 173 FMTYDVKLDPICTAYAKRIMAMPEMQEWITAAK 205


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.132    0.397 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 662,084
Number of extensions: 23458
Number of successful extensions: 78
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 3
Length of query: 225
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 143
Effective length of database: 1,231,600
Effective search space: 176118800
Effective search space used: 176118800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 129 (54.3 bits)