BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0304.p (413 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313879|ref|YP_003777971.1| major facilitator superfamily p... 821 0.0 gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 431 e-122 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 90 3e-19 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 85 1e-17 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 84 2e-17 >gi|300313879|ref|YP_003777971.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 413 Score = 821 bits (2120), Expect = 0.0, Method: Compositional matrix adjust. Identities = 413/413 (100%), Positives = 413/413 (100%) Query: 1 MFSWYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLV 60 MFSWYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLV Sbjct: 1 MFSWYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLV 60 Query: 61 ASAIGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEW 120 ASAIGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEW Sbjct: 61 ASAIGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEW 120 Query: 121 AAGAVLMAETIRAEHRGKAMGTVQSAWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLL 180 AAGAVLMAETIRAEHRGKAMGTVQSAWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLL Sbjct: 121 AAGAVLMAETIRAEHRGKAMGTVQSAWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLL 180 Query: 181 PALLVVYVRRSVQETDAFLAGQKAAASKPAASTLLGIFRPDVLRLTIIGSLLGVGAHGGY 240 PALLVVYVRRSVQETDAFLAGQKAAASKPAASTLLGIFRPDVLRLTIIGSLLGVGAHGGY Sbjct: 181 PALLVVYVRRSVQETDAFLAGQKAAASKPAASTLLGIFRPDVLRLTIIGSLLGVGAHGGY 240 Query: 241 YALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRIGRRRTVAFFALAC 300 YALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRIGRRRTVAFFALAC Sbjct: 241 YALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRIGRRRTVAFFALAC 300 Query: 301 VATVLCYIFLPLSDTQMLVCGFPLGFFAAGIPASMGALFNELYPSGIRGTGVGFCYNFGR 360 VATVLCYIFLPLSDTQMLVCGFPLGFFAAGIPASMGALFNELYPSGIRGTGVGFCYNFGR Sbjct: 301 VATVLCYIFLPLSDTQMLVCGFPLGFFAAGIPASMGALFNELYPSGIRGTGVGFCYNFGR 360 Query: 361 VASAGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVVVAVLMLPETKGRKMETSA 413 VASAGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVVVAVLMLPETKGRKMETSA Sbjct: 361 VASAGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVVVAVLMLPETKGRKMETSA 413 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 431 bits (1109), Expect = e-122, Method: Compositional matrix adjust. Identities = 216/410 (52%), Positives = 285/410 (69%) Query: 4 WYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLVASA 63 W ++ T ER+T A F+G+A+D D +++ IP L+AA+S+SKA+AG I+ L+ SA Sbjct: 2 WLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSA 61 Query: 64 IGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEWAAG 123 IGGW AG L+DRYGRV+ LQ T++WF+FFTFLS F SF QL +A+QGFG GGEWA G Sbjct: 62 IGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAVG 121 Query: 124 AVLMAETIRAEHRGKAMGTVQSAWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLLPAL 183 +VL+AETI A HRGKA G VQS+WAVGW G+ L F ++++LP E AW+V+F IG+LPAL Sbjct: 122 SVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPAL 181 Query: 184 LVVYVRRSVQETDAFLAGQKAAASKPAASTLLGIFRPDVLRLTIIGSLLGVGAHGGYYAL 243 L +Y+RR++ E +LA Q L IF D+LR T++ SLL G G YY++ Sbjct: 182 LTLYIRRAISEPAVYLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYSV 241 Query: 244 MTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRIGRRRTVAFFALACVAT 303 TWLPT+LKTE+HLSVL T YL V+I +CG + S+ L D IGRRR FA + Sbjct: 242 TTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIVL 301 Query: 304 VLCYIFLPLSDTQMLVCGFPLGFFAAGIPASMGALFNELYPSGIRGTGVGFCYNFGRVAS 363 VL Y + ++D ML GF LGFF +GI + MGA +EL+PS +RG+G GFCYNFGR Sbjct: 302 VLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAVG 361 Query: 364 AGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVVVAVLMLPETKGRKMETSA 413 A P LVG++S+S+PLGQ IG AA Y LV+VA L+LPET+G+++E A Sbjct: 362 AICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLVLPETRGKELEAVA 411 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 90.1 bits (222), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 95/335 (28%), Positives = 146/335 (43%), Gaps = 33/335 (9%) Query: 56 GATLVASAIGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVL------- 108 G + +G GA SDR GR L ++I+ + T + AF S+ + +L Sbjct: 67 GVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIAL 126 Query: 109 -KALQGFGFGGEWAAGAVLMAETIRAEHRGKAMGTVQSAWAVGWGGAVLLFSVLFS---- 163 K +QGF GGE+ + + E A HR S+W V G LL + LF Sbjct: 127 GKMIQGFSAGGEFGSSTAFLVE--HAPHRRGFF----SSWQVASQGISLLLAALFGAVLN 180 Query: 164 --LLPQEMA---WRVMFVIGLLPALLVVYVRRSVQETDAFLAGQKAAASKPAASTLLGIF 218 L P+++A WRV F+ GLL A +Y+RR + E F +S+ + L F Sbjct: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEF-----KESSEKTNAPLRDTF 235 Query: 219 RPDVLRLTIIGSLLGVGAHGGYYALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCI 278 +RL I + + Y +L ++PTY + L S+ ++V + + G Sbjct: 236 AHQKMRLLIGAGSVIMATVSVYLSL--YIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSP 293 Query: 279 ASSQLLDRIGRRR-TVAFFALACVATVLCYIFLPLSD--TQMLVCGFPLGFFAAGIPASM 335 L D+IGR + AL V T ++FL S Q+L+ +G A+M Sbjct: 294 LVGALSDKIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAM 353 Query: 336 GALFNELYPSGIRGTGVGFCYNFGRVASAGFPVLV 370 AL +++P RGTG+ YN GF L+ Sbjct: 354 PALLADIFPVATRGTGMSLAYNIAVTIFGGFAGLI 388 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 84.7 bits (208), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 112/423 (26%), Positives = 181/423 (42%), Gaps = 51/423 (12%) Query: 23 WALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLVASAIGGWFAGALSDRYGRVKTL 82 W DA+ V + P + F L+ A V +G + G L+DR GR L Sbjct: 32 WLADAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGMLLGAFVFGRLADRIGRRPVL 91 Query: 83 QITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEWAAGAVLMAETIRAEHRGKAMGT 142 ++ + F SAFA F LL L+ L G G GG +MAE + + RG+ + Sbjct: 92 MGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMMAEFLPSARRGRWLVM 151 Query: 143 VQSAWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLLPALLVVYVRRSVQETDAFL--A 200 ++S WA+G +L V S AWRV+F + LPAL+ V +R V E+ +L + Sbjct: 152 LESFWAIGTICLAVLALVAVSW--GNDAWRVIFFVTGLPALVGVVLRFYVPESPMYLNRS 209 Query: 201 GQKAAASK--------PAASTLLGIFRPDVLRLTIIGSLLGVGAHG-------------- 238 G+ AA + +ST + +P+V+ + +L Sbjct: 210 GKSDAARQVLERVAKVNGSSTPIPPLQPEVVERKPMSALFSAALRRRSLSLYLAWGLISI 269 Query: 239 GYYALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRIGRRRT-VAFFA 297 YY + +LP L +E + +L + + G S+ ++R GR+ T V F Sbjct: 270 AYYGVFVYLPVKLGSE-GFGFMRGQIFLIFLALVQLPGYALSAYGVERWGRKPTLVGFLV 328 Query: 298 LACVATVLCYIFLPLSDTQMLVCGFPLGFFAAGIP--ASMGALFNELYPSGIRGTGVGFC 355 L+ V C ++ L MLV G L + + A++ A E+YP+ +R +G+G Sbjct: 329 LSAVG---CMLY-SLGKDPMLVVGSTLLLSFSLLGTWAALYAFTPEVYPTDLRASGMG-- 382 Query: 356 YNFGRVASAG--------FPVLVGHMSDSMPLGQAIGIDAAIAYSLVVVAVLMLPETKGR 407 G VA G PV+ H + ++ + A + A+A V V E++ R Sbjct: 383 -TAGAVARFGGLFAPAIVAPVMASHFTLALAMLSAFLVAGAVAILCVDV------ESRNR 435 Query: 408 KME 410 +E Sbjct: 436 ALE 438 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 84.3 bits (207), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 101/391 (25%), Positives = 162/391 (41%), Gaps = 55/391 (14%) Query: 26 DALDVQMFSLAIPALIAAFSLSKADAGLISGATLVASAIGGWFAGALSDRYGRVKTLQIT 85 DA + +P L+ + LS A GL+ + + IG F G +++RYGRV + +T Sbjct: 56 DAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQVIGALFFGWMAERYGRVPSASVT 115 Query: 86 IIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEWAAGAVLMAETIRAEHRGKAMGTVQS 145 + S + + A SF L + + LQG G GGE A + E +A RGK + Sbjct: 116 VGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPVAATYINELSQAHGRGKFFVLYEL 175 Query: 146 AWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLLPALLVVYV----------------- 188 + +G +L + + + L W MF++G +P +LV + Sbjct: 176 IFPLG----LLAAAQVGAFLVPRFGWEYMFLVGGIPGILVALLITRLPESPRWLIARGRL 231 Query: 189 ---------------RRSVQ-ETDAFLAGQK-----AAASKPAASTLLGIFRPDVLRLTI 227 +R++ +TD Q+ A A K + +F P TI Sbjct: 232 DDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGKRQRGSWKELFSPVYRGRTI 291 Query: 228 IGSLLGVGAHGGYYALMTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRI 287 I LL ++ + WLP+ KT HL + + +V V C + + L+DR+ Sbjct: 292 IVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCALLIDRV 351 Query: 288 GRRRTVAFFALACVATVLCYIFLPLSDTQMLVCGFPLGFFAAGIPASMGA------LFN- 340 GRRR A + +L L L + + F A+ A MG L+ Sbjct: 352 GRRR------WAIASFILSGALLALLGVLKAESAWSVMFLASSAYAVMGTTTVLLYLYTP 405 Query: 341 ELYPSGIRGTGVGFCYNFGRVASAGFPVLVG 371 E+YP+ +R G G ++ R ASA P +VG Sbjct: 406 EIYPTRMRAIGTGLATSWLRAASAAAPAMVG 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.139 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,198,958 Number of extensions: 48980 Number of successful extensions: 370 Number of sequences better than 1.0e-08: 29 Number of HSP's gapped: 327 Number of HSP's successfully gapped: 29 Length of query: 413 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 325 Effective length of database: 1,203,190 Effective search space: 391036750 Effective search space used: 391036750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)