BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0303.p
         (278 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane h...   559   e-160
gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herb...   124   9e-30
gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillu...    83   2e-17

>gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane
           hydrolase [Herbaspirillum seropedicae SmR1]
          Length = 278

 Score =  559 bits (1440), Expect = e-160,   Method: Compositional matrix adjust.
 Identities = 275/278 (98%), Positives = 277/278 (99%)

Query: 1   MSQPCATPATVLRIDTHAHVFTRDLPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQ 60
           MSQPCATPATVLRIDTHAHVFTRDLPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQ
Sbjct: 1   MSQPCATPATVLRIDTHAHVFTRDLPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQ 60

Query: 61  PSFLGSDNRYLLAAIARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPD 120
           PSFLGSDNRYLLAA+ARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPD
Sbjct: 61  PSFLGSDNRYLLAALARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPD 120

Query: 121 FTGVWRSTLAHIAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDPG 180
           FTGVWRSTLA IAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDPG
Sbjct: 121 FTGVWRSTLARIAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDPG 180

Query: 181 FRYLLSTAAGGQVWVKLSASYRNGGAEQGQRFARQAWPLLREHFGAQRLLWGSDWPHTQH 240
           FRYLLS+AAGGQVWVKLSASYRNGGAEQGQRFARQAWPLLREHFGAQRLLWGSDWPHTQH
Sbjct: 181 FRYLLSSAAGGQVWVKLSASYRNGGAEQGQRFARQAWPLLREHFGAQRLLWGSDWPHTQH 240

Query: 241 ESLTDYGHSWQQFTQLVSDEEQRRIITGQTAAQLFGIN 278
           ESLTDYGHSWQQFTQLVSDEEQRRIITGQTAAQLFGIN
Sbjct: 241 ESLTDYGHSWQQFTQLVSDEEQRRIITGQTAAQLFGIN 278


>gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 309

 Score =  124 bits (311), Expect = 9e-30,   Method: Compositional matrix adjust.
 Identities = 93/280 (33%), Positives = 130/280 (46%), Gaps = 7/280 (2%)

Query: 2   SQPCATPATVLRIDTHAHVFTRDLPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQP 61
           SQP    A +L  D+H H++    P++   R       TV  Y   +   G++  V+VQP
Sbjct: 32  SQPPRLKAPMLACDSHHHIYDARFPVSPHWRGGRPDGATVADYRLLMAKLGIARHVVVQP 91

Query: 62  SFLGSDNRYLLAAIARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDF 121
           S  GSDNR LL A+ ++  + RGI ++D DV   QL  +   GV G+R N +  A     
Sbjct: 92  STYGSDNRCLLDALTQFGAQARGIVVIDDDVDLPQLKEMDRLGVRGVRVNFL-SAQSWGV 150

Query: 122 TGV--WRSTLAHIAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDP 179
           T V   R T   IA LGW V++      + +    L A+  +VV+DH GR+    G+D P
Sbjct: 151 TTVERLRRTAERIAPLGWHVQVLMSGAQIAQHEAVLAALPTQVVIDHLGRIPQPDGLDHP 210

Query: 180 GFRYLLSTAAGGQVWVKLSASYRNGGAEQGQRFARQAWPLLREHFGA--QRLLWGSDWPH 237
           G + +L   A G  W+KLS  Y +    QG         L + +  A  QR LWGSDWPH
Sbjct: 211 GAQAVLRLLAKGNTWIKLSEPYAD--TLQGPPDYPDTSLLAKAYLQAAPQRTLWGSDWPH 268

Query: 238 TQHESLTDYGHSWQQFTQLVSDEEQRRIITGQTAAQLFGI 277
              +   D    +        D   R+ I     A LFG 
Sbjct: 269 PTEKIKPDDAVLFDLLAAWAPDPALRQQILVSNPAALFGF 308


>gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillum
           seropedicae SmR1]
          Length = 315

 Score = 83.2 bits (204), Expect = 2e-17,   Method: Compositional matrix adjust.
 Identities = 74/268 (27%), Positives = 113/268 (42%), Gaps = 15/268 (5%)

Query: 14  IDTHAHVFTRDLPLADVRR--YAPTYDVTVEQYIARLDTHGMSHGVLVQPSFLGSDNRYL 71
            D H H++      A   +  +AP       Q   RL   G +  V+V PS  G DN   
Sbjct: 55  CDCHMHIYDPRFAWAPGAKLVHAPATVAMYRQLQQRL---GTTRNVVVTPSAYGVDNSCT 111

Query: 72  LAAIARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDFTGVWRSTLAH 131
           L A+A+  +  RG+A+V+  VS+ +L  L   GV G+RFNL  G+       +       
Sbjct: 112 LDALAQLGRSARGVAVVNDQVSDAELQRLHQAGVRGLRFNLAVGSVT--TVEMMEPLARR 169

Query: 132 IAQLGW--QVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDPGFRYLLSTAA 189
           +A LGW  Q  +  E +   R +  L  + + +V DHF R+        P F ++     
Sbjct: 170 MAPLGWHLQANMPNEVLLAHRAM--LARLPVPLVFDHFARIPLQADATHPVFDFVTGLMR 227

Query: 190 GGQVWVKLSASYRNGGAEQGQRFARQAWPLLRE--HFGAQRLLWGSDWPHTQHESLTDYG 247
             +  VKLS +Y    +++G        PL R        +L+WGSDWPH   +   D  
Sbjct: 228 ERRASVKLSGAYLY--SKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDA 285

Query: 248 HSWQQFTQLVSDEEQRRIITGQTAAQLF 275
                    +  EE  R +     A+L+
Sbjct: 286 RLLDVMISWLGSEEMVRTVLVANPARLY 313


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.323    0.137    0.429 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,013,034
Number of extensions: 46692
Number of successful extensions: 166
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 162
Number of HSP's successfully gapped: 3
Length of query: 278
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 194
Effective length of database: 1,222,130
Effective search space: 237093220
Effective search space used: 237093220
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 130 (54.7 bits)