BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0302.p (516 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313877|ref|YP_003777969.1| methyl-accepting chemotaxis sen... 1027 0.0 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 462 e-131 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 441 e-125 gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sen... 436 e-123 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 429 e-121 >gi|300313877|ref|YP_003777969.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 539 Score = 1027 bits (2655), Expect = 0.0, Method: Compositional matrix adjust. Identities = 514/516 (99%), Positives = 515/516 (99%) Query: 1 MDRKIATKLLLSFVAVLMLMSCLGVFSVYQMDKVNDAATEIAHKWEPSVRISLTLEKVLA 60 MDRKIATKLLLSFVAVLMLMSCLGVFSVYQMDKVNDAATEIAHKWEPSVRISLTLEKVLA Sbjct: 24 MDRKIATKLLLSFVAVLMLMSCLGVFSVYQMDKVNDAATEIAHKWEPSVRISLTLEKVLA 83 Query: 61 RVRSTEFQHILGNQESMATLEKALTERYAEFTQLQQDYRKLPLTPEEAASFDQVSKTLAA 120 RVRSTEFQHILGNQESMATLEKALTERYAEFTQLQQDYRKLPLTPEE ASFDQVSKTLAA Sbjct: 84 RVRSTEFQHILGNQESMATLEKALTERYAEFTQLQQDYRKLPLTPEETASFDQVSKTLAA 143 Query: 121 YLVEHRKIIALSHDNRKDEALALTKGDSLKAYRAIDKQFATLRQSSIDHKEEANTLADQI 180 YLVEHRKIIALSHDNRKDEALALTKGDSLKAYRAIDKQFATLRQSSIDHKEEANTLADQI Sbjct: 144 YLVEHRKIIALSHDNRKDEALALTKGDSLKAYRAIDKQFATLRQSSIDHKEEANTLADQI 203 Query: 181 YDSSQHWIIGLLLAGIALGLALAIAVARIVSQPLAQALQLARQVADNDLTGQVRVHSADE 240 YDSSQHWIIGLLLAGIALGLALAIA+ARIVSQPLAQALQLARQVADNDLTGQVRVHSADE Sbjct: 204 YDSSQHWIIGLLLAGIALGLALAIAIARIVSQPLAQALQLARQVADNDLTGQVRVHSADE 263 Query: 241 TGQLMQALNHMTQSLGNMVAEVHRSISVIDTASGEIASGNADLSARTESQASSLEETASA 300 TGQLMQALNHMTQSLGNMVAEVHRSISVIDTASGEIASGNADLSARTESQASSLEETASA Sbjct: 264 TGQLMQALNHMTQSLGNMVAEVHRSISVIDTASGEIASGNADLSARTESQASSLEETASA 323 Query: 301 MEELTSTVRQNADHAHQANELVATSSRLAVQGGAVVDQVVQTMGEIRTGSARIADITGVI 360 MEELTSTVRQNADHAHQANELVATSSRLAVQGGAVVDQVVQTMGEIRTGSARIADITGVI Sbjct: 324 MEELTSTVRQNADHAHQANELVATSSRLAVQGGAVVDQVVQTMGEIRTGSARIADITGVI 383 Query: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAREIKALIEGSVQQV 420 DGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAREIKALIEGSVQQV Sbjct: 384 DGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAREIKALIEGSVQQV 443 Query: 421 ATGAELVDQAGQTMHEIVDSVARVATLMSEIAVASREQSRGIDEINHAVTQMDEGTQQNA 480 ATGAELVDQAGQTMHEIVDSVARVATLMSEIAVASREQSRGIDEINHAVTQMDEGTQQNA Sbjct: 444 ATGAELVDQAGQTMHEIVDSVARVATLMSEIAVASREQSRGIDEINHAVTQMDEGTQQNA 503 Query: 481 ALVEQAAAAAHSLRDQTRQLAALVARFRLRGMAHAS 516 ALVEQAAAAAHSLRDQTRQLAALVARFRLRGMAHAS Sbjct: 504 ALVEQAAAAAHSLRDQTRQLAALVARFRLRGMAHAS 539 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 462 bits (1190), Expect = e-131, Method: Compositional matrix adjust. Identities = 252/479 (52%), Positives = 335/479 (69%), Gaps = 1/479 (0%) Query: 2 DRKIATKLLLSFVAVLMLMSCLGVFSVYQMDKVNDAATEIAHKWEPSVRISLTLEKVLAR 61 D +I+ KL+ +F+ V+ +++ LGVFS+ Q+D+VN A+TEI+ W PS+R L+ ++R Sbjct: 6 DLRISRKLITTFLVVVAMVAALGVFSIRQLDQVNSASTEISTNWLPSIRTLARLQLAMSR 65 Query: 62 VRSTEFQHILG-NQESMATLEKALTERYAEFTQLQQDYRKLPLTPEEAASFDQVSKTLAA 120 +RS E QHIL + + +E+ + + Q +Y PEE A + +V K +A Sbjct: 66 LRSFELQHILAKDAKEYEEVERGMQVQMNNLKTAQAEYVTQISEPEEKAIYPEVEKLIAT 125 Query: 121 YLVEHRKIIALSHDNRKDEALALTKGDSLKAYRAIDKQFATLRQSSIDHKEEANTLADQI 180 ++ EH KIIALS + +EA L +G+S KAYR +Q L + + +N AD I Sbjct: 126 FVSEHDKIIALSRQGKDEEARNLQRGESTKAYRTSLQQIDKLAAVNDKGADISNKQADAI 185 Query: 181 YDSSQHWIIGLLLAGIALGLALAIAVARIVSQPLAQALQLARQVADNDLTGQVRVHSADE 240 Y ++ WII + A I L + LA+ V+R++++PL +A+ +A++VA DLT ++ DE Sbjct: 186 YAQARLWIIVTVAACIGLAMLLAVWVSRLIARPLEEAVSVAQRVAGGDLTTRIEPAGKDE 245 Query: 241 TGQLMQALNHMTQSLGNMVAEVHRSISVIDTASGEIASGNADLSARTESQASSLEETASA 300 TGQL+ AL M SL +V +V I TAS EIASGN DLS+RTE QASSLEETASA Sbjct: 246 TGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDLSSRTEEQASSLEETASA 305 Query: 301 MEELTSTVRQNADHAHQANELVATSSRLAVQGGAVVDQVVQTMGEIRTGSARIADITGVI 360 MEELTSTV+QNAD+A QAN+L ++S +A QGGAVV+QVV TMG I S +IADI GVI Sbjct: 306 MEELTSTVKQNADNARQANQLAVSASEVASQGGAVVNQVVDTMGSINASSRKIADIIGVI 365 Query: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAREIKALIEGSVQQV 420 DGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA AA+EIK LIEGSV QV Sbjct: 366 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIEGSVAQV 425 Query: 421 ATGAELVDQAGQTMHEIVDSVARVATLMSEIAVASREQSRGIDEINHAVTQMDEGTQQN 479 G++LV++AG TM E+V SV RV ++ EI+ AS+EQS GI+++N A+ QMDE TQQN Sbjct: 426 DMGSKLVERAGITMSEVVQSVRRVTDIVGEISSASQEQSEGIEQVNLAIAQMDEVTQQN 484 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 441 bits (1135), Expect = e-125, Method: Compositional matrix adjust. Identities = 262/510 (51%), Positives = 359/510 (70%), Gaps = 5/510 (0%) Query: 4 KIATKLLLSFVAVLMLMSCLGVFSVYQMDKVNDAATEIAHKWEPSVRISLTLEKVLARVR 63 KIA KL++SF AV++L LG+F++ ++ VN A+T++A W P+++ + +++ L R+R Sbjct: 8 KIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQASLPRMR 67 Query: 64 STEFQHILGNQESMATLE---KALTERYAEFTQLQQDYRKLPLTPEEAASFDQVSKTLAA 120 +E Q + + A E KA+ R + + Y EE +++ SK+ A Sbjct: 68 ISELQMVTA--ATPAEREDALKAIKSRLDILAKQRAAYEAQISEAEEKVLYEKFSKSFAT 125 Query: 121 YLVEHRKIIALSHDNRKDEALALTKGDSLKAYRAIDKQFATLRQSSIDHKEEANTLADQI 180 YL +++ +S + +++EA AL KGDS K +R++ + + + + + ++ +A+Q+ Sbjct: 126 YLEIDKELARISAEGKEEEARALFKGDSNKTFRSMIENMDEIIKVNDAGSKRSDDMAEQV 185 Query: 181 YDSSQHWIIGLLLAGIALGLALAIAVARIVSQPLAQALQLARQVADNDLTGQVRVHSADE 240 Y S+ WIIGLL A + + L LA+ VAR VS+PL +A+ +A +VA DLT +R S DE Sbjct: 186 YRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLTMDIRATSTDE 245 Query: 241 TGQLMQALNHMTQSLGNMVAEVHRSISVIDTASGEIASGNADLSARTESQASSLEETASA 300 TG LM +L M SL +V EV I TAS EIA+GN DLS+RTE QA SLEETASA Sbjct: 246 TGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQAGSLEETASA 305 Query: 301 MEELTSTVRQNADHAHQANELVATSSRLAVQGGAVVDQVVQTMGEIRTGSARIADITGVI 360 MEELT+TV+QNAD+A QAN+L A++S +A QGGAVV QVVQTM I S RIADI VI Sbjct: 306 MEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESSNRIADIISVI 365 Query: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAREIKALIEGSVQQV 420 DGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA+AA+EIK LI+ SV++V Sbjct: 366 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQLIDDSVEKV 425 Query: 421 ATGAELVDQAGQTMHEIVDSVARVATLMSEIAVASREQSRGIDEINHAVTQMDEGTQQNA 480 G++LV++AG TM E+V SV RV +++EI+ AS EQS GI+EIN A+TQMDE TQQNA Sbjct: 426 GAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINRAITQMDEVTQQNA 485 Query: 481 ALVEQAAAAAHSLRDQTRQLAALVARFRLR 510 ALVE+AAAAA SL+DQ +L+ +V+ F+L+ Sbjct: 486 ALVEEAAAAAQSLQDQAARLSQVVSVFKLQ 515 >gi|300309735|ref|YP_003773827.1| methyl-accepting chemotaxis sensory transducer protein [Herbaspirillum seropedicae SmR1] Length = 567 Score = 436 bits (1122), Expect = e-123, Method: Compositional matrix adjust. Identities = 255/515 (49%), Positives = 344/515 (66%), Gaps = 2/515 (0%) Query: 2 DRKIATKLLLSFVAVLMLMSCLGVFSVYQMDKVNDAATEIAHKWEPSVRISLTLEKVLAR 61 +R+IATKLLL+F VL+L LG+FS+ Q+ KVN AATE+A W PS+R ++ L+R Sbjct: 6 NRRIATKLLLAFACVLLLTGGLGIFSIVQLHKVNQAATELATNWLPSIRSLNDMKVALSR 65 Query: 62 VRSTEFQHIL--GNQESMATLEKALTERYAEFTQLQQDYRKLPLTPEEAASFDQVSKTLA 119 VR+ + Q L G+++ + + + + + ++ Y P E A F V+ + Sbjct: 66 VRANQAQLALYDGDEKQSSAVIGLMNKNLEALAEARKQYTGQISEPGEKALFPGVNARID 125 Query: 120 AYLVEHRKIIALSHDNRKDEALALTKGDSLKAYRAIDKQFATLRQSSIDHKEEANTLADQ 179 ++ H +A + DEA L G + + Y ++ L Q ++D ++ A+ Sbjct: 126 KFIALHGDTLAAVRAGKLDEAKRLLLGPAFENYLSLLNDLDKLAQVNLDGSVQSTRSAET 185 Query: 180 IYDSSQHWIIGLLLAGIALGLALAIAVARIVSQPLAQALQLARQVADNDLTGQVRVHSAD 239 YD +Q W++ +L+ + GLALA+ +AR+++ PL A + A +A DLT + S D Sbjct: 186 TYDQAQVWVVAILIGALLAGLALALFIARVIATPLRSAGEFAHLIAAGDLTADITARSDD 245 Query: 240 ETGQLMQALNHMTQSLGNMVAEVHRSISVIDTASGEIASGNADLSARTESQASSLEETAS 299 ETGQL AL M SL +V++V I TASG+I+SGN DLS+RTE QASSLEETAS Sbjct: 246 ETGQLTAALAAMNTSLRRIVSQVRSGTDAIATASGQISSGNLDLSSRTEEQASSLEETAS 305 Query: 300 AMEELTSTVRQNADHAHQANELVATSSRLAVQGGAVVDQVVQTMGEIRTGSARIADITGV 359 AMEELTSTVRQNA++A QAN+L ++S +A +GG VV QV++TMG I S +I DI V Sbjct: 306 AMEELTSTVRQNAENARQANQLALSASTIAEEGGKVVSQVIETMGSIDASSKKIVDIISV 365 Query: 360 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAREIKALIEGSVQQ 419 ID IAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSA AA+EIK LI+ SVQQ Sbjct: 366 IDSIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSATAAKEIKTLIDNSVQQ 425 Query: 420 VATGAELVDQAGQTMHEIVDSVARVATLMSEIAVASREQSRGIDEINHAVTQMDEGTQQN 479 V G+ LV +AG T+ E+V SV RV +MSEI+ AS EQ++GI+++N A+TQMDE TQQN Sbjct: 426 VGLGSGLVQKAGVTISEVVSSVQRVTDVMSEISAASGEQTQGIEQVNQAITQMDEVTQQN 485 Query: 480 AALVEQAAAAAHSLRDQTRQLAALVARFRLRGMAH 514 AALVE+AA+A+ SL Q ++L+ LV FRL G A Sbjct: 486 AALVEEAASASQSLSHQAQRLSELVGVFRLPGAAR 520 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 429 bits (1104), Expect = e-121, Method: Compositional matrix adjust. Identities = 257/507 (50%), Positives = 341/507 (67%), Gaps = 1/507 (0%) Query: 4 KIATKLLLSFVAVLMLMSCLGVFSVYQMDKVNDAATEIAHKWEPSVRISLTLEKVLARVR 63 IA KL LSF V+ L G+F++ ++ +VN A+ EI KW P++ + L+ L +R Sbjct: 8 NIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQGSLPPLR 67 Query: 64 STEFQHILG-NQESMATLEKALTERYAEFTQLQQDYRKLPLTPEEAASFDQVSKTLAAYL 122 E + + + A KA+ +R T+ + + + P+E + Q K L AY+ Sbjct: 68 LNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFLKNLTAYV 127 Query: 123 VEHRKIIALSHDNRKDEALALTKGDSLKAYRAIDKQFATLRQSSIDHKEEANTLADQIYD 182 ++ ++ + + DE AL GDS K YR++ + + + +A+ A ++ Sbjct: 128 KVDEQLAEMAANQQYDETRALFNGDSAKLYRSMVGNLDAIVKINTSGSAQADEEASTVFR 187 Query: 183 SSQHWIIGLLLAGIALGLALAIAVARIVSQPLAQALQLARQVADNDLTGQVRVHSADETG 242 +Q WI+GLL A + + LA VA VS+PL +A+Q+A++VA DLT ++R DETG Sbjct: 188 GAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARGDLTLRIRPAGRDETG 247 Query: 243 QLMQALNHMTQSLGNMVAEVHRSISVIDTASGEIASGNADLSARTESQASSLEETASAME 302 LM+AL M +SL ++V+EV + I TAS EIA GN DLS RTE QA SLEETASAME Sbjct: 248 ALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGSLEETASAME 307 Query: 303 ELTSTVRQNADHAHQANELVATSSRLAVQGGAVVDQVVQTMGEIRTGSARIADITGVIDG 362 ELTSTV QNAD+A QAN+L AT+S +A QGG VV QVVQTM I S RIADI VIDG Sbjct: 308 ELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRIADIISVIDG 367 Query: 363 IAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAREIKALIEGSVQQVAT 422 IAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA AA+EIK LI+ SVQ+V+ Sbjct: 368 IAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIDDSVQKVSA 427 Query: 423 GAELVDQAGQTMHEIVDSVARVATLMSEIAVASREQSRGIDEINHAVTQMDEGTQQNAAL 482 G+ LV++AG TM E+V SV RV +++EI+ A++EQS GI+E+N A+TQMDE TQQNAAL Sbjct: 428 GSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQMDETTQQNAAL 487 Query: 483 VEQAAAAAHSLRDQTRQLAALVARFRL 509 VEQAAAAA SL+ Q LAA+V +F+L Sbjct: 488 VEQAAAAADSLKGQASNLAAVVGKFKL 514 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.125 0.327 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,035,307 Number of extensions: 31849 Number of successful extensions: 496 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 252 Number of HSP's successfully gapped: 72 Length of query: 516 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 427 Effective length of database: 1,198,455 Effective search space: 511740285 Effective search space used: 511740285 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)