BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0296.p (433 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirill... 869 0.0 gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-... 259 4e-70 gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-... 65 9e-12 gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [... 64 2e-11 >gi|300313871|ref|YP_003777963.1| thiophene oxidase [Herbaspirillum seropedicae SmR1] Length = 433 Score = 869 bits (2246), Expect = 0.0, Method: Compositional matrix adjust. Identities = 432/433 (99%), Positives = 433/433 (100%) Query: 1 MHLAAFLIAGNAAHSQALWRHPQSRRGGFLELDYYLHIARTLERGKFDLLFFADRLAVST 60 MHLAAFLIAGNAAHSQALWRHPQSRRGGFLELDYYLHIARTLERGKFDLLFFADRLAVST Sbjct: 1 MHLAAFLIAGNAAHSQALWRHPQSRRGGFLELDYYLHIARTLERGKFDLLFFADRLAVST 60 Query: 61 RYGDSHLHGLYVGDQDATRLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLD 120 RYGDSHLHGLY+GDQDATRLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLD Sbjct: 61 RYGDSHLHGLYIGDQDATRLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLD 120 Query: 121 HLSGGRAAWNVVTSVNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQ 180 HLSGGRAAWNVVTSVNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQ Sbjct: 121 HLSGGRAAWNVVTSVNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQ 180 Query: 181 QDRYADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVVFN 240 QDRYADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVVFN Sbjct: 181 QDRYADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVVFN 240 Query: 241 IQPDLPRLQAFYRDLKNRAQQFGRRPQDIRILSAVMPFVGQSRQHARELQEQANALADPL 300 IQPDLPRLQAFYRDLKNRAQQFGRRPQDIRILSAVMPFVGQSRQHARELQEQANALADPL Sbjct: 241 IQPDLPRLQAFYRDLKNRAQQFGRRPQDIRILSAVMPFVGQSRQHARELQEQANALADPL 300 Query: 301 VGLSTLSSHMNVDFSGFALDAPLGDVQVSGIQGLFKLLRAISAERQLTLADAGRLYAQGV 360 VGLSTLSSHMNVDFSGFALDAPLGDVQVSGIQGLFKLLRAISAERQLTLADAGRLYAQGV Sbjct: 301 VGLSTLSSHMNVDFSGFALDAPLGDVQVSGIQGLFKLLRAISAERQLTLADAGRLYAQGV 360 Query: 361 LTPQIAGTAADVADWLQSIIVANGADGFVISPSHLPYGFDDFVDQVIPELQRRGAFRQDY 420 LTPQIAGTAADVADWLQSIIVANGADGFVISPSHLPYGFDDFVDQVIPELQRRGAFRQDY Sbjct: 361 LTPQIAGTAADVADWLQSIIVANGADGFVISPSHLPYGFDDFVDQVIPELQRRGAFRQDY 420 Query: 421 AGRQLRQHLAASP 433 AGRQLRQHLAASP Sbjct: 421 AGRQLRQHLAASP 433 >gi|300314031|ref|YP_003778123.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 259 bits (661), Expect = 4e-70, Method: Compositional matrix adjust. Identities = 163/444 (36%), Positives = 232/444 (52%), Gaps = 30/444 (6%) Query: 1 MHLAAFLIAGNAAHSQALWRHPQSRRGGFLELDYYLHIARTLERGKFDLLFFADRLAVST 60 + L AF + + + LW HP+ R + ++ Y+ +AR LERGKFD LF AD + V Sbjct: 6 IRLNAFNMNCVSHINHGLWTHPRDRSQDYTDIAYWTDLARLLERGKFDGLFLADIVGV-- 63 Query: 61 RYGDSHLHGLYVGDQDATRL--------DPLPILGALAAATSHIGLGATRSTTYSQPYSL 112 + +Y G+ D T DPL ++ A+AA T H+G G T + TY P+ Sbjct: 64 -------YDVYQGNTDLTLRETIQLPVNDPLMLVPAMAAVTEHLGFGVTANLTYEAPFLF 116 Query: 113 AREFATLDHLSGGRAAWNVVTSVNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWH-S 171 AR +TLDHLS GR WN+VT A GL E L HD RYDRADEF+EV +KLW S Sbjct: 117 ARRMSTLDHLSRGRVGWNIVTGYLDSAARAMGLHEQLEHDQRYDRADEFMEVVYKLWEGS 176 Query: 172 WQPDALRLQQDR--YADADKVAAIDHQGAHFSVRGPLNIPASPQGHPVIIQAGSSDRGQD 229 W+ A+ + R YAD KV I H G ++ V G S Q PV+ QAGSS RGQ Sbjct: 177 WEDGAVLRDRARRVYADPAKVHKIAHHGDYYHVEGIHLSEPSRQRTPVLYQAGSSGRGQA 236 Query: 230 FAARWAEVVFNIQPDLPRLQAFYRDLKNRAQQFGRRPQDIRILSAVMPFVGQSRQHAREL 289 F AR AE VF + + +++ A + GR P I+I + G++ Q A+E Sbjct: 237 FGARHAECVFISSQSKDATRKLVQSIRDGAVREGRDPDAIKIFMGLTVVTGRTEQQAQER 296 Query: 290 QEQANALADPLVGLSTLSSHMNVDFSGFALDAPLGDVQVSGIQGLFKLLRA----ISAER 345 + A P GL+ S+ +DFS + LD P+ + + I+ L K++ + + Sbjct: 297 YREYARYASPEAGLAHYSAGTGIDFSQYELDEPIRPAKTNAIESLTKVVTQPELNWTKRK 356 Query: 346 QLTLADAGRLYAQGVLTPQIAGTAADVADWLQSIIVANGADGFVISPSHLPYGFDDFVDQ 405 L + G YA I G VAD L+S + G DG+ ++ + P F+DFV+ Sbjct: 357 LLQQLELGGRYA------SIVGAPDRVADELESWVADTGIDGYNLTRTVTPESFEDFVEL 410 Query: 406 VIPELQRRGAFRQDYAGRQLRQHL 429 VIP LQ RG+++++YA LR+ L Sbjct: 411 VIPVLQERGSYKREYAQGTLREKL 434 >gi|300310284|ref|YP_003774376.1| coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase [Herbaspirillum seropedicae SmR1] Length = 385 Score = 65.5 bits (158), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 72/270 (26%), Positives = 117/270 (43%), Gaps = 43/270 (15%) Query: 32 LDYYLHIARTLERGKFDLLFFADRLAVSTRYGDSHLHGLYVGDQDATRLDPLPILGALAA 91 L+Y +A T E+ F+ + ++ + YG + H + + AL Sbjct: 43 LEYNQRLAVTAEKAGFE--YALSQIRFTAGYGAEYQH------------ESVSFSQALLH 88 Query: 92 ATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGRAAWNVVTSVNQGEADNFGLKETLPH 151 AT+ + + A P +A++ AT+DH+S GR A NVV+ +GE G + L H Sbjct: 89 ATTKLKVLAAILPGPWHPAVVAKQIATIDHISNGRIAINVVSGWFKGEFQAIG-EPWLEH 147 Query: 152 DVRYDRADEFLEVTHKLWHSWQPDALRLQQDRYADADKVAAIDHQGAHFSVRGPLNIPAS 211 D RY R++EF+ +W D + D Y + + P+ P Sbjct: 148 DERYRRSNEFIRALKGIWTQ---DDFTFKGDFYRFNNYTLSPK----------PVQKP-- 192 Query: 212 PQGHPVIIQAGSSDRGQDFAARWAEVVFNIQPDLPRLQAFYRDLKNRAQQFGRRPQDIRI 271 HP I Q GSS +D AA ++ F L L+A D++ +A Q G + +RI Sbjct: 193 ---HPEIFQGGSSRAARDNAASVSDWYFTNGNTLEGLKAQIDDIQAKAAQNGHK---VRI 246 Query: 272 LSAVMPFVGQSRQHARELQEQANALADPLV 301 V FV AR+ +E+A A+ ++ Sbjct: 247 --GVNAFV-----IARDTEEEAQAVLQDII 269 >gi|300310902|ref|YP_003774994.1| alkanesulfonate monooxygenase [Herbaspirillum seropedicae SmR1] Length = 375 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 59/191 (30%), Positives = 83/191 (43%), Gaps = 26/191 (13%) Query: 77 ATRLDPLPILGALAAATSHIGLGATRSTTYSQPYSLAREFATLDHLSGGRAAWNVVTS-- 134 A+ LDP + +L T I L T+ P AR+ ATLD GR NVV Sbjct: 53 ASCLDPWVVAASLVPVTQRIKLLVALRTSLGGPGPSARQAATLDQALNGRLLLNVVPGGD 112 Query: 135 VNQGEADNFGLKETLPHDVRYDRADEFLEVTHKLWHSWQPDALRLQQDRYADADKVAAID 194 + EAD LK HD RY+ ADE+L + KL LQ + ++ Sbjct: 113 ATELEADGVFLK----HDERYEAADEYLTIWRKL----------LQGE---------TVN 149 Query: 195 HQGAHFSVR-GPLNIPASPQGHPVIIQAGSSDRGQDFAARWAEVVFNIQPDLPRLQAFYR 253 ++G HF +R G L PA Q +P + GSS D AA+ + ++ Sbjct: 150 YEGKHFRIRNGQLFHPAVQQPYPPLYFGGSSPAAHDLAAKHVDAYLTWGEPPAAVRQKID 209 Query: 254 DLKNRAQQFGR 264 D++ RA + GR Sbjct: 210 DVRERAARQGR 220 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,499,434 Number of extensions: 67894 Number of successful extensions: 192 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 188 Number of HSP's successfully gapped: 5 Length of query: 433 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 345 Effective length of database: 1,203,190 Effective search space: 415100550 Effective search space used: 415100550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)