BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0295.p (323 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate A... 652 0.0 gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporte... 250 9e-68 gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate A... 241 7e-65 gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporte... 220 1e-58 gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate A... 130 1e-31 >gi|300313870|ref|YP_003777962.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 323 Score = 652 bits (1682), Expect = 0.0, Method: Compositional matrix adjust. Identities = 322/323 (99%), Positives = 322/323 (99%) Query: 1 MDAHQPRHKRRLLLQGLAAGALASGTPLVRAAADKVLRIGYQKFNTLNILKGSGKLEQAL 60 MDAHQPRHKRRLLLQGLAAGALASGTPLVRAAADKVLRIGYQKFNTLNILKGSGKLEQAL Sbjct: 1 MDAHQPRHKRRLLLQGLAAGALASGTPLVRAAADKVLRIGYQKFNTLNILKGSGKLEQAL 60 Query: 61 QPQGWSVKWFEFAAGPQLTEALNAQAIDLGHAADTPSVFANASGVNAVYLGAEQPYPKGI 120 QPQGWSVKWFEFAAGPQLTEALNA AIDLGHAADTPSVFANASGVNAVYLGAEQPYPKGI Sbjct: 61 QPQGWSVKWFEFAAGPQLTEALNANAIDLGHAADTPSVFANASGVNAVYLGAEQPYPKGI 120 Query: 121 GIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVRA 180 GIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVRA Sbjct: 121 GIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVRA 180 Query: 181 AFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVF 240 AFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVF Sbjct: 181 AFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVF 240 Query: 241 AELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIADA 300 AELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIADA Sbjct: 241 AELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIADA 300 Query: 301 FLKLGLIKNPVRVQDKVYPEVLV 323 FLKLGLIKNPVRVQDKVYPEVLV Sbjct: 301 FLKLGLIKNPVRVQDKVYPEVLV 323 >gi|300310977|ref|YP_003775069.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 327 Score = 250 bits (639), Expect = 9e-68, Method: Compositional matrix adjust. Identities = 139/317 (43%), Positives = 191/317 (60%), Gaps = 4/317 (1%) Query: 4 HQPRHKRRLLLQGLAAGAL--ASGTPLVRAAADKVLRIGYQKF-NTLNILKGSGKLEQAL 60 HQ R + L +A GAL A G V+A K+LRIGYQK NTL +LK G LE+ L Sbjct: 10 HQARRRAIQRLSAIALGALTIALGASPVQAQETKLLRIGYQKAANTLVLLKAHGTLEKRL 69 Query: 61 QPQGWSVKWFEFAAGPQLTEALNAQAIDLGHAADTPSVFANASGVNAVYLGAEQPYPKGI 120 QP G VKW EFAAGPQL EALN ++D G+ + P VFA A+G + VY E P P Sbjct: 70 QPLGVEVKWAEFAAGPQLLEALNVGSVDFGYVGEAPPVFAQAAGADFVYTAYEIPTPDAE 129 Query: 121 GIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVRA 180 G+ V + SPI+SVA+LKGKK+A +G +V +L++ L++ GL Y DI+PVY+ AD RA Sbjct: 130 GLLVQKNSPIKSVAELKGKKIAFNKGSDVHWLVVALLKKAGLQYSDIQPVYLA-PADARA 188 Query: 181 AFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVF 240 AF+ G VDA +WDPF A E R L G G N+ F L+ F ++ + + + Sbjct: 189 AFERGAVDAWAIWDPFQAAAEKQVGARRLATGVGAVNHHQFFLSARPFAAKNPQVEKILL 248 Query: 241 AELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIADA 300 E+ + +W ++H + A LAP G+D ++ +R + VDA ++AEQQ+IADA Sbjct: 249 EEISREGEWVRTHTAQAAAQLAPIQGLDADIIETGLKRYAHVYKPVDAQVLAEQQKIADA 308 Query: 301 FLKLGLIKNPVRVQDKV 317 F +L LI P++V D V Sbjct: 309 FYELKLIPKPLKVSDAV 325 >gi|300309984|ref|YP_003774076.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 241 bits (614), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 137/321 (42%), Positives = 185/321 (57%), Gaps = 9/321 (2%) Query: 1 MDAHQPRHKRRLLLQGLAAGALASGTPL----VRAAADKVLRIGYQKFNTL-NILKGSGK 55 M H RR LL AA AL G+ L V A +K +RIGYQK +TL ILK +G Sbjct: 1 MSKHSTDSTRRRLLH--AATALGLGSALAPASVFAQGNKAIRIGYQKSSTLLTILKANGT 58 Query: 56 LEQALQPQGWSVKWFEFAAGPQLTEALNAQAIDL-GHAADTPSVFANASGVNAVYLGAEQ 114 LE+ L P G + W EFA+G L EALN +DL ADT VFA A+G Y+ E Sbjct: 59 LEKLLAPTGAKISWHEFASGLPLLEALNVGGVDLSADVADTVPVFAQAAGAKLAYVAQES 118 Query: 115 PYPKGIGIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTN 174 P P I V +S +R +DLKGKK+A + V +LL+ LE+ GL + D++ Y++ Sbjct: 119 PSPAAQAIVVRADSVLRKASDLKGKKIAVTKAAGVHYLLIATLEKAGLKFSDVDAAYLSP 178 Query: 175 AADVRAAFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKD 234 A D RAAF+ G+VDA WDPFLAG + ++ RIL DG G+++ + ++LA SF + Sbjct: 179 A-DGRAAFERGSVDAWVTWDPFLAGVQRQSQVRILSDGRGVADYQRYYLASASFAQARPE 237 Query: 235 IVRTVFAELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQ 294 ++R VF EL+KT W + HP+E A LAP G+D + LA RR+Y AV + EQ Sbjct: 238 VLRVVFDELQKTGHWVKQHPKEAAAFLAPLWGLDADTIELANSRRSYEVRAVRPEALGEQ 297 Query: 295 QQIADAFLKLGLIKNPVRVQD 315 Q+IAD F GL+ + D Sbjct: 298 QRIADTFFAAGLLPRKINTND 318 >gi|300310915|ref|YP_003775007.1| alkanesulfonates ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 220 bits (560), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 119/291 (40%), Positives = 169/291 (58%), Gaps = 2/291 (0%) Query: 29 VRAAADKVLRIGYQKFNTLNILKGSGKLEQALQPQGWSVKWFEFAAGPQLTEALNAQAID 88 V AAA + LR+GYQK L++++ G LEQ L P G +VKW EF+AGP EAL A +ID Sbjct: 45 VHAAAPRQLRVGYQK-GALSLIRAHGVLEQRLAPLGATVKWVEFSAGPVQLEALGAGSID 103 Query: 89 LGHAADTPSVFANASGVNAVYLGAEQPYPKGIGIFVAQESPIRSVADLKGKKVATGRGWN 148 G + P +FA A+G VY+GA P P + V +S ++ LKGK++A +G N Sbjct: 104 FGDVGEAPPIFAQAAGTPLVYVGATVPRPYVEAVLVKDDSELQRPQQLKGKRIALNKGSN 163 Query: 149 VQFLLLKALEEVGLSYRDIEPVYVTNAADVRAAFQSGNVDAATLWDPFLAGQELATRPRI 208 V + L+K LE+ GL Y D+ PV++ AD RAAF+ G VDA +W+P+LA + + RI Sbjct: 164 VHYFLVKLLEKHGLKYGDVTPVWLA-PADARAAFERGAVDAWAIWEPYLAAAQQGLKARI 222 Query: 209 LRDGTGLSNNRTFHLAVPSFVDRHKDIVRTVFAELKKTNQWTQSHPQETAELLAPQLGVD 268 L D G+ NR ++ + + H D++R EL K + W Q+ P E A LA LG+ Sbjct: 223 LADADGVVKNRAYYFSTRDYARDHADLLRIALEELGKIDAWAQARPDEYASELAGILGLP 282 Query: 269 PKVLRLATERRNYTTVAVDAGIVAEQQQIADAFLKLGLIKNPVRVQDKVYP 319 VL + R +Y T+ + I+ EQQQIAD FL L LI + + + P Sbjct: 283 RSVLDVVVRRYHYGTLPITRAILDEQQQIADTFLDLRLIPRKISLLEAAPP 333 >gi|300309986|ref|YP_003774078.1| nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 328 Score = 130 bits (328), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 102/310 (32%), Positives = 158/310 (50%), Gaps = 14/310 (4%) Query: 8 HKR---RLLLQGLAAGALASGTPLVRAAAD--KVLRIGYQKFNTLN-ILKGSGKLEQALQ 61 H+R RL L AL G PL+ +AA KV+R+ Y ++ + +L+ G LE+ L+ Sbjct: 2 HRRDFLRLAPAALGVTALG-GIPLLSSAAQPPKVIRLDYAYYSPPSLVLRKFGWLEEDLK 60 Query: 62 PQGWSVKWFEFAAGPQLTEALNAQAIDLGHAADTPSVFANASG--VNAVYLGAEQPYPKG 119 QG VKW + E LN+ ++D G A ++ A A+G V AVY+ + P+ Sbjct: 61 AQGTEVKWVLSQGSNRALEYLNSDSVDFGSTAGLAALLARANGNPVKAVYIYSR---PEW 117 Query: 120 IGIFVAQESPIRSVADLKGKKVATGRGWNVQFLLLKALEEVGLSYRDIEPVYVTNAADVR 179 + V +ESP++S+ +LKGKK+A +G + LL+AL E GL D+E V++ + AD R Sbjct: 118 TALAVGKESPVKSINELKGKKIAATKGTDPYLFLLRALHENGLKKSDVEIVHLQH-ADGR 176 Query: 180 AAFQSGNVDAATLWDPFLAGQELATRPRILRDGTGLSNNRTFHLAVPSFVDRHKDIVRTV 239 AA + G V A DP LA EL R++ N F SF RH D V+ V Sbjct: 177 AALEQGRVAAWAGLDPHLAASELEAGSRLIYRNVNF-NTYGFLNVSDSFAARHPDQVKRV 235 Query: 240 FAELKKTNQWTQSHPQETAELLAPQLGVDPKVLRLATERRNYTTVAVDAGIVAEQQQIAD 299 A ++ W +HP ET LL+ + + V RL +R +++ + + + A Sbjct: 236 IAAYERARSWIIAHPDETVALLSEESKLSAPVARLQLKRNDFSQPQIGREHIEALRAAAP 295 Query: 300 AFLKLGLIKN 309 L+ L+K+ Sbjct: 296 ILLEEDLVKS 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 994,503 Number of extensions: 42144 Number of successful extensions: 160 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 7 Length of query: 323 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 238 Effective length of database: 1,217,395 Effective search space: 289740010 Effective search space used: 289740010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)