BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0291.p (476 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313868|ref|YP_003777960.1| major facilitator superfamily p... 916 0.0 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 125 8e-30 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 116 4e-27 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 100 2e-22 gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 80 3e-16 >gi|300313868|ref|YP_003777960.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 916 bits (2367), Expect = 0.0, Method: Compositional matrix adjust. Identities = 472/476 (99%), Positives = 474/476 (99%) Query: 1 LSAAASLPARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSIGSVLERPDTLGLDAAQ 60 LSAAASLPARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSIGSVLERPDTLGLDAAQ Sbjct: 3 LSAAASLPARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSIGSVLERPDTLGLDAAQ 62 Query: 61 VGWTGSLYIAGAVVGALVFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWFALCRL 120 VGWTGSLYIAGAVVGALVFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWFALCRL Sbjct: 63 VGWTGSLYIAGAVVGALVFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWFALCRL 122 Query: 121 ATGLGIGGEYAAINSAIDELVPARVRGRVNLVINASFWIGAALGAGLSLVILDERVLGPV 180 ATGLGIGGEYAAINSAIDELVPARVRGRVNLVINASFWIGAALGAGLSLVILDERVLGPV Sbjct: 123 ATGLGIGGEYAAINSAIDELVPARVRGRVNLVINASFWIGAALGAGLSLVILDERVLGPV 182 Query: 181 MGWRVCFLLGAVLAVAIVLVRRHVPESPRWLAMHGHHARAEAVVREIEAQVMAEHGTLPA 240 MGWRVCFLLGAVLAVAIVLVRRHVPESPRWLAMHGHHARAEAVVREIEAQVMAEHGTLPA Sbjct: 183 MGWRVCFLLGAVLAVAIVLVRRHVPESPRWLAMHGHHARAEAVVREIEAQVMAEHGTLPA 242 Query: 241 LPTAAPGEPSRSPGQAAGLLSWREIAQVLLHHYRARSLMVALMMVAQAFVYNAIFFTYSL 300 LPTAAPGE SRSPGQA GLLSWREIAQVLLHHYRARSLMVALMMVAQAFVYNAIFFTYSL Sbjct: 243 LPTAAPGESSRSPGQAVGLLSWREIAQVLLHHYRARSLMVALMMVAQAFVYNAIFFTYSL 302 Query: 301 VLTRFFQVPDTRVALYLFPFALGNVLGPLLLGHLFDSIGRRRMIAATYLLAGLGLAATAA 360 VLTRFFQVPDTRVALYLFPFALGNVLGPLLLGHLFDSIGRRRMIAATYLLAG+GLAATAA Sbjct: 303 VLTRFFQVPDTRVALYLFPFALGNVLGPLLLGHLFDSIGRRRMIAATYLLAGVGLAATAA 362 Query: 361 AFNAGWLDALGLALCWSAVFFVASAAASSAYLTASEVFPLQMRAMAIAIFYAIGTGVGGF 420 AFNAGWLDALGLALCWSAVFFVASAAASSAYLTASEVFPLQMRAMAIAIFYAIGTGVGGF Sbjct: 363 AFNAGWLDALGLALCWSAVFFVASAAASSAYLTASEVFPLQMRAMAIAIFYAIGTGVGGF 422 Query: 421 IAPALLGWLIASGERQAVAAGYAVGAVLALLAGLCALRWAVDAERRTLEEVAELKR 476 IAPALLGWLIASG+RQAVAAGYAVGAVLALLAGLCALRWAVDAERRTLEEVAELKR Sbjct: 423 IAPALLGWLIASGDRQAVAAGYAVGAVLALLAGLCALRWAVDAERRTLEEVAELKR 478 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 125 bits (314), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 144/501 (28%), Positives = 215/501 (42%), Gaps = 81/501 (16%) Query: 4 AASLPARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSIGSVLERPDTLGL---DAAQ 60 AA L R++ +P+SRWH + I +G A + D S+ VL P +GL AAQ Sbjct: 26 AAGLLFRIENVPFSRWHTKARIIMGSATLFDAFHAL---SLAFVL--PVLVGLWQLSAAQ 80 Query: 61 VGWTGSLYIAGAVVGALVFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWFALCRL 120 +G + G V+GAL FG + +R GR +T+ L ++ A F LCR Sbjct: 81 IGLLIASGYLGQVIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRF 140 Query: 121 ATGLGIGGEYAAINSAIDELVPARVRGRVNLVINASFWIG--AALGAGLSLVILDERVLG 178 G+G+GGE + I+EL A RG+ ++ F +G AA G LV Sbjct: 141 LQGIGVGGEVPVAATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLV-------- 192 Query: 179 PVMGWRVCFLLGAVLAVAIVLVRRHVPESPRWLAMHGHHARAEAVVREIEAQV------- 231 P GW FL+G + + + L+ +PESPRWL G AE ++R +EA Sbjct: 193 PRFGWEYMFLVGGIPGILVALLITRLPESPRWLIARGRLDDAEKLIRTLEASTPKRNLDA 252 Query: 232 -MAEHGTLPALPTAAPGEPSRSPGQAAGLLSWREIAQVLLHHYRARSLMVALMMVAQAFV 290 + + A G R G SW+E+ + YR R+++V L+ + FV Sbjct: 253 QTDKQAIEQRINALAAGAGKRQRG------SWKELFSPV---YRGRTIIVWLLWASSYFV 303 Query: 291 YNAIFFTYSLVLTRFFQVP---DTRVALYLFPFALGNVLGPLLLGHLFDSIGRRRMIAAT 347 N I + + +P R+A + VLG L L D +GRRR A+ Sbjct: 304 ANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCAL---LIDRVGRRRWAIAS 360 Query: 348 YLLAGLGLAATAAAFNAGWLDALGLALCWSAVFFVASAAASSA------YLTASEVFPLQ 401 ++L+G LA WS +F +SA A YL E++P + Sbjct: 361 FILSGALLALLGVLKAES---------AWSVMFLASSAYAVMGTTTVLLYLYTPEIYPTR 411 Query: 402 MRAMAIAIFYAIGTGVGGFIAPALLGWLIASGERQA-----VAAGYAVGAVLALLAGLCA 456 MR AIGTG+ WL A+ V AG+ + V + AG+ Sbjct: 412 MR--------AIGTGLA-------TSWLRAASAAAPAMVGVVLAGHGIATVFLMFAGVTV 456 Query: 457 L----RW-AVDAERRTLEEVA 472 L W ++ R+LEE++ Sbjct: 457 LGLLAAWRMIETTNRSLEEIS 477 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 116 bits (290), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 79/240 (32%), Positives = 124/240 (51%), Gaps = 12/240 (5%) Query: 18 RWHWRVVIALGVAWVLDGLEVTLVGSIGSVLERPDTLGLDAAQVGWTGSLYIAGAVVGAL 77 ++ +R+ + G+ W+ D ++V +G + + T GL Q TG+++ G ++GA Sbjct: 19 KFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAK--TFGLTVPQALQTGTMFFVGMLLGAF 76 Query: 78 VFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWFALCRLATGLGIGGEYAAINSAI 137 VFGRL DR+GR+ + M + + +ASAFA DF W RL TG+G+GG + + Sbjct: 77 VFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMM 136 Query: 138 DELVPARVRGRVNLVINASFW-IGAALGAGLSLVILDERVLGPVMGWRVCFLLGAVLAVA 196 E +P+ RGR LV+ SFW IG A L+LV + WRV F + + A+ Sbjct: 137 AEFLPSARRGR-WLVMLESFWAIGTICLAVLALVAVSWG----NDAWRVIFFVTGLPALV 191 Query: 197 IVLVRRHVPESPRWLAMHGHHARAEAVVREIEAQVMAEHGTLPALPTAAPGEPSRSPGQA 256 V++R +VPESP +L G A R++ +V +G+ +P P R P A Sbjct: 192 GVVLRFYVPESPMYLNRSGKSDAA----RQVLERVAKVNGSSTPIPPLQPEVVERKPMSA 247 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 100 bits (249), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 66/220 (30%), Positives = 112/220 (50%), Gaps = 8/220 (3%) Query: 11 LDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSIGSVLERPDTLGLDAAQVGWTGSLYIA 70 +D ++++HW V+ + V DG ++ + G++ VL + +L Q G GS + Sbjct: 10 IDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSL--SPVQAGALGSYALF 67 Query: 71 GAVVGALVFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWFALCRLATGLGIGGEY 130 G + GA +FG L DR+GRKK I+ L+ +T S FAT F + R GLG GG Sbjct: 68 GMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGLGCGGLM 127 Query: 131 AAINSAIDELVPARVRGRVNLVINASFWIGAALGAGLSLVILDERVLGPVMGWRVCFLLG 190 + ++E P R+R + ++ + + +G L AGL + +L P GW+ F Sbjct: 128 PNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWML------PNFGWQSMFFAA 181 Query: 191 AVLAVAIVLVRRHVPESPRWLAMHGHHARAEAVVREIEAQ 230 AV V + ++ +PES +L G +A A++ +++ Q Sbjct: 182 AVPLVLLPVILWKLPESVGFLLRQGRLEQARAMLTQVDPQ 221 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 80.5 bits (197), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 114/427 (26%), Positives = 176/427 (41%), Gaps = 67/427 (15%) Query: 56 LDAAQVGWTGSLYIAGAVVGALVFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWF 115 + A+ G+ + + + +G G L DR GR ++ T+ + T S F T F Sbjct: 44 MSKAEAGYIATGALLTSAIGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQL 103 Query: 116 ALCRLATGLGIGGEYAAINSAIDELVPARVRGRVNLVINASFWIGAALGAGLSLVILDER 175 R G G+GGE+A + I E + AR RG+ + ++ +S+ +G A G+ L+ + Sbjct: 104 FFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQSSWAVGWA-GSALAFWGVYA- 161 Query: 176 VLGPVMGWRVCFLLGAVLAVAIVLVRRHVPESPRWLAMHGHHARAEAVVREIEAQVMAEH 235 VL P W+V F +G + A+ + +RR + E +LA +A +RE Sbjct: 162 VLPPETAWKVLFWIGVLPALLTLYIRRAISEPAVYLA-------TQARLRE--------- 205 Query: 236 GTLPALPTAAPGEPSRSPGQAAGLLSWREIAQVLLHHYRARSLMVALMMVA--QAFVYNA 293 GQ L + H R+ ++A ++ Q Y+ Sbjct: 206 -----------------SGQRGNFLR-------IFHRDLLRTTLLASLLATGMQGAYYSV 241 Query: 294 IFFTYSLVLT-RFFQVPDTRVALYLFPFALGNVLGPLLLGHLFDSIGRRRMIAATYLLAG 352 + + + T R V T + YL G+ G L L D+IGRRR L A Sbjct: 242 TTWLPTFLKTERHLSVLGT--STYLLVLIGGSFCGYLTSAWLSDTIGRRRCFM---LFAA 296 Query: 353 LG----LAATAAAFNAGWLDALGLALCWSAVFFVASAAASSAYLTASEVFPLQMRAMAIA 408 +G L+ T W+ ALG L F + AYL SE+FP +R Sbjct: 297 MGIVLVLSYTQMIITDAWMLALGFVL---GFFLSGIFSGMGAYL--SELFPSDVRGSGQG 351 Query: 409 IFYAIGTGVGGFIAPALLGWLIASGERQAVAAGYAVGAVLALLAGLCALRWAVDAERR-- 466 Y G VG I PAL+G+L ++ G +G + A GL + V E R Sbjct: 352 FCYNFGRAVGA-ICPALVGYL-----SNSLPLGQTIGWIAAGCYGLVIVACLVLPETRGK 405 Query: 467 TLEEVAE 473 LE VA+ Sbjct: 406 ELEAVAK 412 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,350,491 Number of extensions: 52478 Number of successful extensions: 514 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 511 Number of HSP's successfully gapped: 18 Length of query: 476 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 387 Effective length of database: 1,198,455 Effective search space: 463802085 Effective search space used: 463802085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)