BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0288.p (487 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 967 0.0 gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 259 3e-70 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 259 5e-70 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 246 2e-66 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 233 3e-62 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 967 bits (2500), Expect = 0.0, Method: Compositional matrix adjust. Identities = 486/487 (99%), Positives = 486/487 (99%) Query: 1 MQASPFLDSAQWQQHLFRGQWVAAAQGTTVLEPATGRPLTQVGLASPDDVSASVELAIAA 60 MQASPFLDSAQWQQHLFRGQWVAAAQG TVLEPATGRPLTQVGLASPDDVSASVELAIAA Sbjct: 1 MQASPFLDSAQWQQHLFRGQWVAAAQGATVLEPATGRPLTQVGLASPDDVSASVELAIAA 60 Query: 61 QAAWVAMPPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAA 120 QAAWVAMPPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAA Sbjct: 61 QAAWVAMPPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAA 120 Query: 121 GMPLQAQGQLLPSTPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKP 180 GMPLQAQGQLLPSTPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKP Sbjct: 121 GMPLQAQGQLLPSTPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKP 180 Query: 181 DLRTPVSGGFMLARIFEQAGLPAGLLHVLPGGAETGEALVTAPGVPMIAFTGSPAVGRRI 240 DLRTPVSGGFMLARIFEQAGLPAGLLHVLPGGAETGEALVTAPGVPMIAFTGSPAVGRRI Sbjct: 181 DLRTPVSGGFMLARIFEQAGLPAGLLHVLPGGAETGEALVTAPGVPMIAFTGSPAVGRRI 240 Query: 241 GELAGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASL 300 GELAGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASL Sbjct: 241 GELAGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASL 300 Query: 301 EEGLLQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYE 360 EEGLLQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYE Sbjct: 301 EEGLLQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYE 360 Query: 361 QLFYQPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVG 420 QLFYQPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVG Sbjct: 361 QLFYQPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVG 420 Query: 421 RAMAVAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIRQ 480 RAMAVAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIRQ Sbjct: 421 RAMAVAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIRQ 480 Query: 481 QAMPYPF 487 QAMPYPF Sbjct: 481 QAMPYPF 487 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 259 bits (663), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 166/424 (39%), Positives = 238/424 (56%), Gaps = 7/424 (1%) Query: 69 PRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQG 128 P +R + AA D+ A+ +A ETG V+ A AA M Q G Sbjct: 62 PGQRRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAG 121 Query: 129 QLLPS-TPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPVS 187 +++PS PG L++ R P GV+ I+P+N P+IL +R +A LA GN VV K P + Sbjct: 122 EVIPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPAT 181 Query: 188 GGFMLARIFEQAGLPAGLLHVLPGGAE----TGEALVTAPGVPMIAFTGSPAVGRRIGEL 243 G ++A + ++AGLP G+++ + E EA++ P V + FTGS VGR I Sbjct: 182 HGLIIAAL-QEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQ 240 Query: 244 AGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEEG 303 RHLK LELGG ++L DADLD A AA+GA+ + GQICM+ RI+V S+ + Sbjct: 241 CARHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADE 300 Query: 304 LLQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYEQLF 363 ++RLA KA LP+GD G V LG ++D ++R +AL+ +++A GA LV GGR Sbjct: 301 FVRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTL 360 Query: 364 YQPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRAM 423 T+L V M ++ EE FGPV ++R DE AIA AN + GL++ + S V RA+ Sbjct: 361 MPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARAL 420 Query: 424 AVAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIRQQAM 483 A++ R+ G+ H+N TV+DE PFGG +G G GG A E+T+ RW T++ Sbjct: 421 ALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFG-HFGGKAGIAEFTELRWVTVQTTPR 479 Query: 484 PYPF 487 YPF Sbjct: 480 HYPF 483 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 259 bits (661), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 167/453 (36%), Positives = 240/453 (52%), Gaps = 9/453 (1%) Query: 13 QQHLFRGQWVAAAQGT--TVLEPATGRPLTQVGLASPDDVSASVELAIAAQAAWVAMPPR 70 QQ GQW A QG V PA G L V L + ++E A AA AW + Sbjct: 12 QQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAK 71 Query: 71 ERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQGQL 130 ER+ I RR L + D+LA ++ E G L + E+ A + A + G Sbjct: 72 ERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDT 131 Query: 131 LPS-TPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPVSGG 189 +PS +P + + +GV I+P+NFP + R PALAAG +V+KP TP S Sbjct: 132 IPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSA- 190 Query: 190 FMLARIFEQAGLPAGLLHVLPGGAE-TGEALVTAPGVPMIAFTGSPAVGRRIGELAGRHL 248 LA + E+AG+PAG+ V+ G + G + + P V I+FTGS VG+ + + + + Sbjct: 191 LALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSI 250 Query: 249 KKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEEGLLQRL 308 KKLSLELGG ++ +DADLD A A + + GQ C+ ANRI V + + +L Sbjct: 251 KKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKL 310 Query: 309 ADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYEQL---FYQ 365 + + VG G V GP+I+E+ +Q+V V ++VA GA+++ GG+ L F++ Sbjct: 311 VEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFE 370 Query: 366 PTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRAMAV 425 PTVL V P M+V EE FGP+A + RF DE+ +ALAN GLA+ S +GR V Sbjct: 371 PTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRV 430 Query: 426 AARLRQGMVHVNDQTVNDECVNPFGGPGIAGNG 458 A L GMV +N +++E V PFGG +G G Sbjct: 431 AEGLESGMVGINTGLISNE-VAPFGGVKQSGLG 462 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 246 bits (629), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 158/471 (33%), Positives = 254/471 (53%), Gaps = 10/471 (2%) Query: 14 QHLFRGQWVAAAQGTT--VLEPATGRPLTQVGLASPDDVSASVELAIAAQAAWVAMPPRE 71 Q G+W A+A G T V+ PAT + ++ A D+ ++E A W M E Sbjct: 6 QLFINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFE 65 Query: 72 RADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQGQLL 131 R+ I RRAA L + +E+AR++ E G L + + E A A + G+L+ Sbjct: 66 RSKIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLV 125 Query: 132 PS-TPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPVSGGF 190 P+ PG + + P+G + +P+NFPL +R ++ ALAAG +++VK TP S Sbjct: 126 PARVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAE 185 Query: 191 MLARIFEQAGLPAGLLHVLPG-GAETGEALVTAPGVPMIAFTGSPAVGRRIGELAGRHLK 249 ++ R + AG+PAG+++++ G AE E L+ P + I+FTGS VG+++ LAG+H+K Sbjct: 186 LI-RAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMK 244 Query: 250 KLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEEGLLQRLA 309 + ++ELGG ++ +DAD+DVAA A + + GQ+C++ R LV + + + Sbjct: 245 RATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFV 304 Query: 310 DKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGR---YEQLFYQP 366 A L VG+G + +GP+ ++R++ + AL+ ++V+ GAQL GG+ + F+QP Sbjct: 305 TYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQP 364 Query: 367 TVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRAMAVA 426 TVL + R EE FGP+A + F+ ++ I ANR GLAA + S A+A Sbjct: 365 TVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALA 424 Query: 427 ARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTT 477 AR+ GM+ +N + + PFGG +G G S GG E Y + T Sbjct: 425 ARVESGMLTINHLGLALPEL-PFGGIKDSGYG-SEGGTEAIEAYINTKLVT 473 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 233 bits (594), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 163/425 (38%), Positives = 237/425 (55%), Gaps = 12/425 (2%) Query: 63 AWVAMPPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEH-EVREAITLCHLAAG 121 AW + +R + + A L + D LA M AR G + +V AI+ AAG Sbjct: 59 AWSKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAG 118 Query: 122 MPLQAQGQLLPST----PGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVV 177 Q +G+ +P+ LS R P+GV+G I P+N PL++T VA LAAG VV Sbjct: 119 WANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVV 178 Query: 178 VKPDLRTPVSGGFMLARIFEQAGLPAGLLHVLPG-GAETGEALVTAPGVPMIAFTGSPAV 236 +KP TP S LAR+ ++AG P G+++V+ G G G AL P V I+FTGSP Sbjct: 179 IKPAEETPQSA-LHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEA 237 Query: 237 GRRIGELAGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILV 296 GR I +AG K+++LELGG + ++ +DA + A G +++QGQ+C A +RILV Sbjct: 238 GRAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILV 297 Query: 297 HASLEEGLLQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAG 356 SL + + LA+ A + VGD + V +GP+ + Q +RV+ +Q+ + GA L+AG Sbjct: 298 QRSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAG 357 Query: 357 G--RYEQ-LFYQPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAG 413 G R ++ F QPT+ GM + EEIFGPV ++I FD +E+AIALAN GLAA Sbjct: 358 GVSRPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAAT 417 Query: 414 VLSPSVGRAMAVAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQW 473 V + ++ RA VAA ++ G V VN + D + P+GG +G G GG A YT+ Sbjct: 418 VWTTNLARAHRVAAGVKVGAVGVNCWSPLDANL-PWGGIKDSGMGRE-GGLAGALAYTEE 475 Query: 474 RWTTI 478 + T+ Sbjct: 476 KTVTV 480 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,856,896 Number of extensions: 91394 Number of successful extensions: 415 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 345 Number of HSP's successfully gapped: 21 Length of query: 487 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 398 Effective length of database: 1,198,455 Effective search space: 476985090 Effective search space used: 476985090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)