BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0287.p (255 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313865|ref|YP_003777957.1| 3-alpha-hydroxysteroid dehydrog... 520 e-149 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 65 7e-12 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 62 6e-11 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 61 8e-11 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 56 3e-09 >gi|300313865|ref|YP_003777957.1| 3-alpha-hydroxysteroid dehydrogenase/carbonyl reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 520 bits (1340), Expect = e-149, Method: Compositional matrix adjust. Identities = 253/255 (99%), Positives = 254/255 (99%) Query: 1 MNLQDKTVVVTGVSSGIGAETARQLRAQGARVIGVDRNEPAISVEAFVQADLSSAEAIDA 60 MNLQDKTVVVTGVSSGIGAETARQLRAQGARVIGVDRNEPAISVE FVQADLSSAEAIDA Sbjct: 1 MNLQDKTVVVTGVSSGIGAETARQLRAQGARVIGVDRNEPAISVETFVQADLSSAEAIDA 60 Query: 61 MVAQLPERIDALCNIAGVPGTAEAQLVARVNYLGLRHLVQRLTARLSPGGAIVNVASVLG 120 MVAQLPERIDALCNIAGVPGTAEAQLVARVNYLGLRHLVQRLTARLSPGGAIVNVAS+LG Sbjct: 61 MVAQLPERIDALCNIAGVPGTAEAQLVARVNYLGLRHLVQRLTARLSPGGAIVNVASILG 120 Query: 121 AEWPQRLEQHKALSQAASFAEGAAWLAQHPVAQEHCYQYFKEALIVWSMTQAQPMFLQHS 180 AEWPQRLEQHKALSQAASFAEGAAWLAQHPVAQEHCYQYFKEALIVWSMTQAQPMFLQHS Sbjct: 121 AEWPQRLEQHKALSQAASFAEGAAWLAQHPVAQEHCYQYFKEALIVWSMTQAQPMFLQHS 180 Query: 181 VRMNCVAPGPVFTPILGDFVTMLGQERVQKDAHRMKRPAYADEIAAAIVWLCSDDSRWIN 240 VRMNCVAPGPVFTPILGDFVTMLGQERVQKDAHRMKRPAYADEIAAAIVWLCSDDSRWIN Sbjct: 181 VRMNCVAPGPVFTPILGDFVTMLGQERVQKDAHRMKRPAYADEIAAAIVWLCSDDSRWIN 240 Query: 241 GVNLPVDGGLASTYL 255 GVNLPVDGGLASTYL Sbjct: 241 GVNLPVDGGLASTYL 255 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 64.7 bits (156), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 70/274 (25%), Positives = 115/274 (41%), Gaps = 57/274 (20%) Query: 3 LQDKTVVVTGVSSGIGAETARQLRAQGARVIGVDRNEPAISVEAFVQA------DLSSAE 56 L KTV++T + GIG + +GARVI D ++P + A + D++ Sbjct: 8 LAGKTVLITAAAQGIGRASTELFAREGARVIATDISKPHLDELAGIAGVETHLLDVTDDA 67 Query: 57 AIDAMVAQLPERIDALCNIAGVPGTAEA------------QLVARVNYLGLRHLVQRLTA 104 AI A+VA++ ID L N AG L A+ + +R ++ + A Sbjct: 68 AIKALVAKIGT-IDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRAVLPGMLA 126 Query: 105 RLSPGGAIVNVASVLGAEWPQRLEQHKALSQAASFAEGAAWLAQHPVAQEHCYQYFKEAL 164 + G+IVN+AS AAS +G VA Y K A+ Sbjct: 127 K--KAGSIVNIAS------------------AASSVKG--------VANRFAYGASKAAV 158 Query: 165 IVWSMTQAQPMFLQHSVRMNCVAPGPVFTPILGDFVTMLGQERVQKDAHR---------M 215 + + + A F+ +R N + PG + +P L ++ +E + + M Sbjct: 159 VGLTKSVAAD-FVAQGIRCNAICPGTIESPSLNQRISTQAKETGKSEEEVRAAFVGRQPM 217 Query: 216 KRPAYADEIAAAIVWLCSDDSRWINGVNLPVDGG 249 R A+E+AA ++L SD+S + G +DGG Sbjct: 218 GRIGKAEEVAALALYLASDESNFTTGSIHMIDGG 251 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 61.6 bits (148), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 71/264 (26%), Positives = 118/264 (44%), Gaps = 32/264 (12%) Query: 1 MNLQDKTVVVTGVS--SGIGAETARQLRAQGARVIGVD--RNEPA----------ISVEA 46 M L+DK ++TG + +G+G TAR + AQGAR++ +D R EPA + + A Sbjct: 1 MLLKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVA 60 Query: 47 FVQADLSSAEAIDAMVAQLPERIDALCNIAGVPGTAEAQLVARVNYLGLRHLVQRLTARL 106 V + A A++ Q RID L N AGV A+ + +Y + + R T + Sbjct: 61 DVTDKAACEAAAAAVLKQF-GRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYM 119 Query: 107 SPGGAIVNVASVLGAEWPQRLEQHKALSQAASFAEGAAWLAQHPVAQEHCYQYFKEALIV 166 S +VL A Q+ +S ++ G H Y K ++ Sbjct: 120 SQ--------AVLPAMQAQQSGSIVCISSVSAQRGGGILGGPH-------YSAAKAGVLG 164 Query: 167 WSMTQAQPMFLQHSVRMNCVAPGPVFTPILGDFVTMLGQERVQKDAHRMKRPAYADEIAA 226 + A+ F +R+NC+ PG + T I D ++ + + + + R D++ Sbjct: 165 LARAMARE-FGPQGIRVNCITPGLISTDINKDILSAEKKAEIAQTIP-LGRLGGPDDVGG 222 Query: 227 AIVWLCSDDSRWINGVNLPVDGGL 250 V+L SD S++ GV L V+GG+ Sbjct: 223 VCVFLASDLSQYCTGVTLDVNGGM 246 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 61.2 bits (147), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 80/279 (28%), Positives = 122/279 (43%), Gaps = 59/279 (21%) Query: 1 MNLQDKTVVVTGVSSGIGAETARQLRAQGARVIGVD---RNEPAISVEAFVQADLSSAEA 57 MN+ DK ++VTG S GIGA TA L Q + V+ A +V A +QA A A Sbjct: 1 MNMADKVILVTGGSRGIGAATA-LLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIA 59 Query: 58 IDAMVAQLPE-------------RIDALCNIAGVPG------TAEAQLVARV-------N 91 + VA+ + R+ AL N AGV A+ + RV + Sbjct: 60 VGGDVAREEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGS 119 Query: 92 YLGLRHLVQRLTARLSP-GGAIVNVASVLGAEWPQRLEQHKALSQAASFAEGAAWLAQHP 150 L R V+R++ R GGAIVN++S+ AA ++ Sbjct: 120 LLCAREAVKRMSTRHGGQGGAIVNLSSM-----------------AAKLGGPGEYV---- 158 Query: 151 VAQEHCYQYFKEALIVWSMTQAQPMFLQHSVRMNCVAPGPVFTPILGDFVTMLGQERVQK 210 Y K A+ +++ A+ + + +R+N V PG ++T I +R+ K Sbjct: 159 -----DYAASKGAIDAFTIGLAKEVAAE-GIRVNAVRPGLIYTDIHASGGEAGRVDRL-K 211 Query: 211 DAHRMKRPAYADEIAAAIVWLCSDDSRWINGVNLPVDGG 249 DA MKR A E+A AI+WL S+++ + G + V GG Sbjct: 212 DAVPMKRGGSAAEVANAILWLLSEEASYATGTFIDVSGG 250 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 55.8 bits (133), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 79/274 (28%), Positives = 117/274 (42%), Gaps = 53/274 (19%) Query: 2 NLQDKTVVVTGVSSGIGAETARQLRAQGARVIGVDRNEP------AISVEA------FVQ 49 L K V G S GIGA ++L A+GA V + P SV+A +Q Sbjct: 14 TLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQ 73 Query: 50 ADLSSAEAIDA---MVAQLPERIDALCNIAGVPGTAEAQLVARVNYLGLRHLVQRLTARL 106 AD + A A+ + A+ R+D L N AGV + ++ L L + L Sbjct: 74 ADSADAAALQQAIRLAAETFGRLDILVNNAGV------LAMGPLDQFKLEDLDRTLA--- 124 Query: 107 SPGGAIVNVASVLGA--EWPQRL-EQHKALSQAASFAEGAAWLAQHPVAQEHCYQYFKEA 163 VNV SV A E + + E + +S ++ A+ + P A Y K A Sbjct: 125 ------VNVRSVFVATQEAARYMGEGGRVISIGSTNAD------RIPFAGGAVYAMSKSA 172 Query: 164 LIVWSMTQAQPMFLQHSVRMNCVAPGPVFT---PILGDFVTMLGQERVQKDAHRMKRPAY 220 L+ + A+ + + +N V PGPV T P GDF L M P Y Sbjct: 173 LVGLTKGLARDLG-PRGITVNNVQPGPVDTDMNPASGDFAASL--------TGLMALPRY 223 Query: 221 --ADEIAAAIVWLCSDDSRWINGVNLPVDGGLAS 252 A+EIA+ + +L +S +I G NL +DGG ++ Sbjct: 224 GKAEEIASFVAYLAGPESGYITGANLMIDGGFSA 257 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.132 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 797,129 Number of extensions: 32341 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 225 Number of HSP's successfully gapped: 8 Length of query: 255 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 172 Effective length of database: 1,226,865 Effective search space: 211020780 Effective search space used: 211020780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)