BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0279.p (565 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) syntheta... 1130 0.0 gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthet... 120 4e-28 >gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) synthetase [Herbaspirillum seropedicae SmR1] Length = 565 Score = 1130 bits (2923), Expect = 0.0, Method: Compositional matrix adjust. Identities = 558/565 (98%), Positives = 562/565 (99%) Query: 1 MLRIAIAQCNPTIGDFEGNLALHLDGLQRAAQAGADLVVFPELSICGYYPGDLLEEARFL 60 MLRIAIAQCNPTIGDFEGNLALHLDGLQRAAQAGADLVVFPELSICGYYPGDLLEEARFL Sbjct: 1 MLRIAIAQCNPTIGDFEGNLALHLDGLQRAAQAGADLVVFPELSICGYYPGDLLEEARFL 60 Query: 61 ARAEATLQDVIDATRRRPGLTAVIGAPRRNPGPGKPLFNALLAVREGRIVAEYHKQLLPV 120 RAEATLQDVIDATRR PGLTAVIGAPRRNPGPGKPLFNALLAVREGRIVAEYHKQLLPV Sbjct: 61 DRAEATLQDVIDATRRLPGLTAVIGAPRRNPGPGKPLFNALLAVREGRIVAEYHKQLLPV 120 Query: 121 YGVFDDGRHFEAGPETACVLPLKGMRVGFLICEDGWNDDLRSYRVNPFEKLAQADAQLVV 180 YGVFDDGRHFEAGPETACVLPLKGMRVGFLICEDGWNDDLRSYRVNPFEKLAQADAQLVV Sbjct: 121 YGVFDDGRHFEAGPETACVLPLKGMRVGFLICEDGWNDDLRSYRVNPFEKLAQADAQLVV 180 Query: 181 SINASPSDVGKRSQRHALFQAACLRRQMALLYVNQVGGQDQLVYDGGSFAMSPLQGLAFE 240 SINASPSDVGKRSQRHALFQAACLRRQMALLYVNQVGGQDQLVYDGGSFAMSPLQGLAFE Sbjct: 181 SINASPSDVGKRSQRHALFQAACLRRQMALLYVNQVGGQDQLVYDGGSFAMSPLQGLAFE 240 Query: 241 AARLAEDFQVLAFEGGRFLRSDGVGAPQCIDQTLATPEFHRRQILLGLRDYARRCGFGKV 300 AARLAEDFQVLAFEGGRFLRSDGVGAPQCIDQT+ATPEFHRRQILLGLRDYARRCGFGKV Sbjct: 241 AARLAEDFQVLAFEGGRFLRSDGVGAPQCIDQTMATPEFHRRQILLGLRDYARRCGFGKV 300 Query: 301 VVGSSGGIDSAVTIALAAEALGAQNVVAVTMPSMFSSAGSIDDSETLCRALGVTLFRHPI 360 VVGSSGGIDSAVTIALAAEALGAQNVVAVTMPS+FSSAGS+ DSETLC+ALGVTLFRHPI Sbjct: 301 VVGSSGGIDSAVTIALAAEALGAQNVVAVTMPSVFSSAGSVSDSETLCQALGVTLFRHPI 360 Query: 361 ADLVREFEDGFATAFGAPLRGLPLENLQARVRGAILMEYSNGYDALLLTTGNKSEISVGY 420 ADLVREFEDGFATAFGAPLRGLPLENLQARVRGAILMEYSNGYDALLLTTGNKSEISVGY Sbjct: 361 ADLVREFEDGFATAFGAPLRGLPLENLQARVRGAILMEYSNGYDALLLTTGNKSEISVGY 420 Query: 421 CTLYGDTNGGLGLIGDLYKTEVYALARHLNAQAGREIIPAAIIDKAPSAELAPDQRDTDS 480 CTLYGDTNGGLGLIGDLYKTEVYALARHLNAQAGREIIPAAIIDKAPSAELAPDQRDTDS Sbjct: 421 CTLYGDTNGGLGLIGDLYKTEVYALARHLNAQAGREIIPAAIIDKAPSAELAPDQRDTDS 480 Query: 481 LPPYEVLDEILKWHIEGSRLPADEAQAAARTVEQLSGTDTGAALVERICRMVARNEYKRR 540 LPPYEVLDEILKWHIEGSRLPADEAQAAARTVEQLSGTDTGAALVERICRMVARNEYKRR Sbjct: 481 LPPYEVLDEILKWHIEGSRLPADEAQAAARTVEQLSGTDTGAALVERICRMVARNEYKRR 540 Query: 541 QSPPVIRLRSRAFGSGRQIPIAVRY 565 QSPPVIRLRSRAFGSGRQIPIAVRY Sbjct: 541 QSPPVIRLRSRAFGSGRQIPIAVRY 565 >gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthetase [Herbaspirillum seropedicae SmR1] Length = 685 Score = 120 bits (300), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 146/590 (24%), Positives = 234/590 (39%), Gaps = 100/590 (16%) Query: 3 RIAIAQCNPTIGDFEGNLALHLDGLQRAAQAGADLVVFPELSICGYYPGDLLEEARFLAR 62 R+A+A + + D N + ++AA GA LV FPEL + Y DL ++ L Sbjct: 15 RVAVAVPDCKVADPGFNAQRTIALAEQAAARGAVLVSFPELGLSAYSCEDLFQQRALLDA 74 Query: 63 AEATLQDVIDATRRRPGLTAVIGAPRRNPGPGKPLFNALLAVREGRIVAEYHKQLLPVYG 122 + LQ V++A+ R P ++G P + L+N + + GRI+ K LP Y Sbjct: 75 SLQALQSVLEASMRIPA-ALIVGLPLK---VDHQLYNCAVVLHHGRILGVVPKSYLPNYS 130 Query: 123 VFDDGRHFEAGPETAC-VLPLKGMRVGF------------------LICEDGWNDDLRSY 163 F + R F + + L G VGF ICED W Sbjct: 131 EFYEARQFSSADCAVTRSVHLLGQEVGFGSHLLFEIRNIPDFRFHIEICEDVW------V 184 Query: 164 RVNPFEKLAQADAQLVVSINASPSDVGKRSQRHALFQAACLRRQMALLYVNQVGGQD--Q 221 + P A A A ++V+++AS VGK RH L R A LY + G+ Sbjct: 185 PIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGESTTD 244 Query: 222 LVYDGGSFAMSPLQGLAFEAARLA-----------------EDFQVLAF------EGGRF 258 L +DG + + LA E+ R A E F F Sbjct: 245 LAWDGQALICENGELLA-ESERFAQGGHVIYADVDLERLSRERFHQTTFGQSVRRHADEV 303 Query: 259 LRSDGVGAPQCIDQTLATP------------------EFHRRQI----LLGLRDYARRCG 296 R + VG I Q A P E R++ + L G Sbjct: 304 RRFEVVGFEVQIPQQQALPLQRRVARFPYVPANAEQRELRCREVYSIQVQALVQRLSSAG 363 Query: 297 FGKVVVGSSGGIDSAVTIALAAEALGA-----QNVVAVTMPSMFSSAGSIDDSETLCRAL 351 KVV+G SGG+DS + + A+A+ N++A TMP +S+ ++ + L + Sbjct: 364 LKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRSNILAYTMPGFATSSRTLVQAHQLMAQV 423 Query: 352 GVTL----FRHPIADLVREFEDGFATAFGAPLRGLPLENLQARVRGAILMEYSNGYDALL 407 G + R ++++ G + G P+ + EN+QA R L +N + A++ Sbjct: 424 GCSAQEIDIRPSCEQMLKDL--GHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAIV 481 Query: 408 LTTGNKSEISVGYCTL-YGDTNGGLGLIGDLYKTEVYALARHLNAQAGREIIPAAIIDKA 466 + TG+ SE+++G+CT GD + + KT + + R + E+ ++ Sbjct: 482 IGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHMVRWVAESGSLELKQPEVLIHV 541 Query: 467 PSAELAPD-----------QRDTDSLPPYEVLDEILKWHIEGSRLPADEA 505 E++P+ QR D + PYE+ D L + + PA A Sbjct: 542 LETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVA 591 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,793,134 Number of extensions: 78561 Number of successful extensions: 251 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 248 Number of HSP's successfully gapped: 3 Length of query: 565 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 475 Effective length of database: 1,193,720 Effective search space: 567017000 Effective search space used: 567017000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)