BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0266.p (423 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-de... 864 0.0 gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein ... 383 e-107 gi|300313762|ref|YP_003777854.1| cytochrome c precursor protein ... 336 2e-93 gi|300311001|ref|YP_003775093.1| cytochrome c subunit II protein... 314 8e-87 gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herb... 283 2e-77 >gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-dehydrogenase cytochrome c subunit [Herbaspirillum seropedicae SmR1] Length = 423 Score = 864 bits (2232), Expect = 0.0, Method: Compositional matrix adjust. Identities = 418/423 (98%), Positives = 419/423 (99%) Query: 1 MKKLTSLFAALAWSLVALGTLTTAQAADQDLVARGQYLARAGDCMACHSAAGKPAYSGGL 60 MKKLTSLFAALAWSL ALGTLTTAQAADQDLVARGQYLARAGDCMACHSAAGKPAYSGGL Sbjct: 1 MKKLTSLFAALAWSLGALGTLTTAQAADQDLVARGQYLARAGDCMACHSAAGKPAYSGGL 60 Query: 61 AIDSGHGIIYSTNITPDKEHGIGNYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDE 120 AIDSGHGIIYSTNITPDKEHGIGNYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDE Sbjct: 61 AIDSGHGIIYSTNITPDKEHGIGNYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDE 120 Query: 121 DIHALYTYFMQGVQPVASTPPASSMSFPFNIRWGMKLWNVFFANDKPFREQDGWSAEIKR 180 DIHALYTYFMQGVQPVASTPPASSMSFPFNIRWGMKLWNVFFANDKPFREQDGWSAEIKR Sbjct: 121 DIHALYTYFMQGVQPVASTPPASSMSFPFNIRWGMKLWNVFFANDKPFREQDGWSAEIKR 180 Query: 181 GAYLVEGLGHCGSCHTPRGFAMNEKASDSSQAQFLSGGDLNGWAVPSLRGMPHWSAQDIV 240 GAYLVEGLGHCGSCHTPRG AMNEKASDSSQAQFLSGGDLNGWAVPSLRGMPHWSAQDIV Sbjct: 181 GAYLVEGLGHCGSCHTPRGVAMNEKASDSSQAQFLSGGDLNGWAVPSLRGMPHWSAQDIV 240 Query: 241 DYLQTGRNKTASVAGEMSLVVEHSTAHLKPEDLQAMAAYLKTLPPVASSGSGRVIPQGVD 300 DYLQTGRNKTASVAGEMSLVVEHST+HLK EDLQAMAAYLKTL PVASSGSGRVIPQGVD Sbjct: 241 DYLQTGRNKTASVAGEMSLVVEHSTSHLKREDLQAMAAYLKTLSPVASSGSGRVIPQGVD 300 Query: 301 ATVSKLTAAKDLTLGERLYLDNCAACHFVTGRGAPGIFPVLDGATVVNAENPSALLHVIL 360 ATVSKLTAAKDLTLGERLYLDNCAACHFVTGRGAPGIFPVLDGATVVNAENPSALLHVIL Sbjct: 301 ATVSKLTAAKDLTLGERLYLDNCAACHFVTGRGAPGIFPVLDGATVVNAENPSALLHVIL 360 Query: 361 AGARTPSTEKAPSILVMPGFAHRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLRAGMD 420 AGARTPSTEKAPSILVMPGFAHRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLRAGMD Sbjct: 361 AGARTPSTEKAPSILVMPGFAHRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLRAGMD 420 Query: 421 KQH 423 KQH Sbjct: 421 KQH 423 >gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 383 bits (983), Expect = e-107, Method: Compositional matrix adjust. Identities = 200/429 (46%), Positives = 267/429 (62%), Gaps = 20/429 (4%) Query: 8 FAALAWSLVALGTLTTAQ-------AADQDLVARGQYLARAGDCMACHSAAGKPAYSGGL 60 F A ++++L LT A +ADQ LV RG+YLA+AGDC+ACH+A G ++GGL Sbjct: 6 FIAACAAVMSLSALTAAAQSNPAAPSADQQLVQRGEYLAKAGDCVACHTAKGGKPFAGGL 65 Query: 61 AIDSGHGIIYSTNITPDKEHGIGNYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDE 120 AI + G +YS+NITPDKE+GIGNYSE F A+RHG+R DG LYPAMPYPSYAKV Sbjct: 66 AIATPIGTVYSSNITPDKENGIGNYSEEDFDRALRHGIRKDGASLYPAMPYPSYAKVKPA 125 Query: 121 DIHALYTYFMQGVQPVASTPPASSMSFPFNIRWGMKLWNVFFANDKPFREQDGW--SAEI 178 D+ ALY YFM GVQ + +++P ++RW + +W FA P DG ++ + Sbjct: 126 DVKALYAYFMHGVQADPAPNRGVDITWPLSMRWPLSIWRKVFA---PAVAVDGPEDNSPL 182 Query: 179 KRGAYLVEGLGHCGSCHTPRGFAMNEKASDSSQAQFLSGGDLNGWAVPSLR-----GMPH 233 RG YLVEGLGHCG+CHTPRG M EKA + +QFLSGG ++G+ +LR G+ + Sbjct: 183 VRGQYLVEGLGHCGACHTPRGVGMQEKALSNDSSQFLSGGVIDGYLANNLRGDGRDGLGN 242 Query: 234 WSAQDIVDYLQTGRNKTASVAGEMSLVVEHSTAHLKPEDLQAMAAYLKTLPPVASSGSGR 293 WS DIV +L+TGRN ++ G M+ VV +ST ++ EDL AMA YLK+L PV Sbjct: 243 WSEADIVAFLKTGRNSHSAAFGGMADVVANSTQYMTEEDLSAMAKYLKSLKPVKDGTPAL 302 Query: 294 VIPQGVDATVSKLTAAKDLTLGERLYLDNCAACHFVTGRGAPGIFPVLDGATVVNAENPS 353 D T L D + G +L+NCAACH +G+G FP L + VNAENP+ Sbjct: 303 AYD---DKTHQALRKGSDQSPGAMAFLNNCAACHRSSGKGYDETFPSLALSPTVNAENPA 359 Query: 354 ALLHVILAGARTPSTEKAPSILVMPGFAHRLSDEEAAALATFVRQGWGNHAGKVSEREVG 413 +L+ ++L GA P T KAP+ MP F RLSD+E A + TF+R WGN A VS +V Sbjct: 360 SLIRIVLEGAEMPWTHKAPTQFAMPAFGSRLSDQEVAEVVTFIRSSWGNQASSVSASDVA 419 Query: 414 KLRAGMDKQ 422 K+R + ++ Sbjct: 420 KVRKQLPEK 428 >gi|300313762|ref|YP_003777854.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 336 bits (861), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 182/415 (43%), Positives = 249/415 (60%), Gaps = 16/415 (3%) Query: 10 ALAWSLVALGTLTTAQAADQDLVARGQYLARAGDCMACHSAAGKPAYSGGLAIDSGHGII 69 ALA + A G+ A A DQ ++ RG YLARAGDC+ACH++ G ++GGLA+ S G I Sbjct: 65 ALAQRIAAQGS---ASAEDQQIL-RGAYLARAGDCVACHTSKGGAPFAGGLALASPIGAI 120 Query: 70 YSTNITPDKEHGIGNYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDEDIHALYTYF 129 YSTNITPDK+HGIG++S F+ +R GV G +YPAMPYPSY++++DED+ ALY YF Sbjct: 121 YSTNITPDKQHGIGDWSYEDFARLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQALYAYF 180 Query: 130 MQGVQPVASTPPASSMSFPFNIRWGMKLW-NVFFANDKPFREQDGWSAEIKRGAYLVEGL 188 + V P A A+ + +P ++RW + LW VF P+ G E+ RGAYLVEGL Sbjct: 181 SKAVPPSAQENRANDIPWPLSMRWPLALWRKVFAPTPAPYTPAAGSDQELARGAYLVEGL 240 Query: 189 GHCGSCHTPRGFAMNEKASDSSQAQ-FLSGGD-LNGWAVPSLR-----GMPHWSAQDIVD 241 GHCGSCH+PR M EKA + FLSGG ++GW+VPSLR G+ WS D+V+ Sbjct: 241 GHCGSCHSPRAVTMQEKALKEDGGRLFLSGGQVVDGWSVPSLRNEHGGGIAGWSQADLVE 300 Query: 242 YLQTGRNKTASVAGEMSLVVEHSTAHLKPEDLQAMAAYLKTLPPVASSGSGRVIPQGVDA 301 +L+TGRN+ + G M+ V+E S ++ DL AMA YL +LPP + R A Sbjct: 301 FLRTGRNQYTASFGAMNDVIEDSMQYMSDADLNAMARYLLSLPPRQQAAPYRYD----SA 356 Query: 302 TVSKLTAAKDLTLGERLYLDNCAACHFVTGRGAPGIFPVLDGATVVNAENPSALLHVILA 361 T + G R+YLD CAACH G G FP L G V+ + ++ + +IL Sbjct: 357 TAQAAYDGRPDGPGARIYLDRCAACHRSNGTGYGKAFPALAGNPVLQTSDATSAIRIILQ 416 Query: 362 GARTPSTEKAPSILVMPGFAHRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLR 416 G R PST A + LVM +A L D++ A + ++++ WGN G + EV K+R Sbjct: 417 GGRQPSTASATAGLVMAPYAQLLDDQQVAEVTSYIQTAWGNRGGTTTAAEVAKVR 471 >gi|300311001|ref|YP_003775093.1| cytochrome c subunit II protein [Herbaspirillum seropedicae SmR1] Length = 453 Score = 314 bits (805), Expect = 8e-87, Method: Compositional matrix adjust. Identities = 180/407 (44%), Positives = 237/407 (58%), Gaps = 27/407 (6%) Query: 24 AQAADQ-DLVARGQYLARAGDCMACHSAAGKPAYSGGLAIDSGHGIIYSTNITPDKEHGI 82 A AAD+ L+ RG+YLA AGDC ACHS G+ +SGG I S G IYSTNITP K GI Sbjct: 24 AHAADEGQLIKRGEYLAIAGDCAACHSQKGRAPFSGGHPISSPLGTIYSTNITPSKTAGI 83 Query: 83 GNYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDEDIHALYTYFMQGVQPVASTPPA 142 G+Y+ QF+AA+R GVRADG+QLYPAMPY +YA+++DED+ ALYTYFMQGV V PA Sbjct: 84 GSYTRDQFAAALRQGVRADGSQLYPAMPYTAYARLNDEDVTALYTYFMQGVAAVDQAAPA 143 Query: 143 SSMSFPFNIRWGMKLWNVFFANDKPFREQDGWSAEIKRGAYLVEGLGHCGSCHTPRGFAM 202 + + FPFNIR MK WN F + KPF SA+ RGAYLV+GL HC +CHTPR F M Sbjct: 144 TDLPFPFNIRLSMKAWNWLFLDKKPFEPDPSQSAQWNRGAYLVQGLAHCSTCHTPRNFLM 203 Query: 203 NEKASDSSQAQFLSGGDLNGWAVP-----SLRGMPHWSAQDIVDYLQTGRNKT-ASVAGE 256 E+ + L GG L W P +L+ P W+ + + DYL +G A+ AG Sbjct: 204 AEQLE-----RRLQGGSLGTWYAPDITPAALQAKPAWTREMLADYLASGHAANGATAAGP 258 Query: 257 MSLVVEHSTAHLKPEDLQAMAAYLKTLPPVASSGSGRVIPQGV-----DATVSKLTAAKD 311 M +E S + L+ EDL+AMA+YL ++ + P G+ D T L A Sbjct: 259 MLEAIEMSFSRLRREDLEAMASYLLPPTAATAAPAVNKPPAGLTPVQGDDTPQALAA--- 315 Query: 312 LTLGERLYLDNCAACHFVTGRGAPGIFPVLDGATVVNAENPSALLHVILAGARTPSTEKA 371 L+ G R+Y++NCA+CH G+G G+ P L V+ N ++ IL G + Sbjct: 316 LSPGARVYMNNCASCHNTAGQGLNGL-PPLQSHPVLRQPNGDNVVMAILEGVLPEHGQG- 373 Query: 372 PSILVMPGFAHRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLRAG 418 M GFAH L+DE+ AA+ ++ Q G V+ + V LRAG Sbjct: 374 -----MIGFAHTLNDEQVAAVTNYLLQDIGGSKVSVTTQRVATLRAG 415 >gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 499 Score = 283 bits (724), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 169/412 (41%), Positives = 228/412 (55%), Gaps = 32/412 (7%) Query: 31 LVARGQYLARAGDCMACHSAA-GKPAYSGGLAIDSGHGIIYSTNITPDKEHGIGNYSEAQ 89 L+ RG YLA A DC+ACH+A GKP +GGLAI S G I +TNITP K HGIGNY+E Q Sbjct: 52 LIERGHYLAIASDCIACHTAPRGKP-MAGGLAIASPVGEIIATNITPSKTHGIGNYTEEQ 110 Query: 90 FSAAVRHGVRADGTQLYPAMPYPSYAKVSDEDIHALYTYFMQGVQPVASTPPASSMSFPF 149 F+AA+R GVRADG LYPAMPY +YA +SD D+ ALY YFM+GV PV + + FP Sbjct: 111 FAAALRRGVRADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPVDVATRPTDLPFPM 170 Query: 150 NIRWGMKLWNVFFANDKPFREQDGWSAEIKRGAYLVEGLGHCGSCHTPRGFAMNEKASDS 209 N+RW MK WN F ++P Q SA+ RG YL EG HC +CHTPRG+ M EK Sbjct: 171 NLRWSMKAWNALFLKEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCHTPRGWLMQEKPDLQ 230 Query: 210 SQAQFLSGGDLNGWAVPSLRGMP-----HWSAQDIVDYLQTGR-NKTASVAGEMSLVVEH 263 L+G + W P++ P WS D+V YL+TGR A AG M+ + H Sbjct: 231 -----LAGAQVGPWYAPNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAAGSMAEAITH 285 Query: 264 STAHLKPEDLQAMAAYLKTLPPVAS---SGSGRVIPQGVDATVSKLTAAKDLT------- 313 S + L EDL+A+A Y+K P +A+ GSG QG + L+ Sbjct: 286 SFSRLTEEDLRAIAVYIKDRPAIATGDAQGSGSRFDQGKAGNALAQFRGRTLSAESPEEI 345 Query: 314 LGERLYLDNCAACHFVTGRGA-PGIFPVLDGATVVNAENPSALLHVILAGARTPSTEKAP 372 G R++ +CA+CH G+G+ G +P L + N + L+ IL G TE+ Sbjct: 346 RGARIFSASCASCHGYNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGVDR-ETEQGG 404 Query: 373 SILVMPGFA------HRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLRAG 418 + MP F ++LS+ + AAL+ ++ + +G A V +V +R G Sbjct: 405 HVF-MPPFGDQPNALNKLSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQG 455 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,410,909 Number of extensions: 62052 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 255 Number of HSP's successfully gapped: 8 Length of query: 423 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 335 Effective length of database: 1,203,190 Effective search space: 403068650 Effective search space used: 403068650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)