BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0265.p (590 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like prot... 1219 0.0 gi|300312639|ref|YP_003776731.1| choline dehydrogenase [Herbaspi... 572 e-164 gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbas... 508 e-145 gi|300313052|ref|YP_003777144.1| choline dehydrogenase [Herbaspi... 83 7e-17 >gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like protein [Herbaspirillum seropedicae SmR1] Length = 590 Score = 1219 bits (3155), Expect = 0.0, Method: Compositional matrix adjust. Identities = 587/590 (99%), Positives = 588/590 (99%) Query: 1 MPDITNDDVDVVIVGLGWAGSLMAIELAQAGLKVRALERGPDRPPEDFAYPKPADQYGYA 60 MPDITNDDVDVVIVGLGWAGSLMAIELAQAGLKVRALERGPDRPPEDFAYPKPADQYGYA Sbjct: 1 MPDITNDDVDVVIVGLGWAGSLMAIELAQAGLKVRALERGPDRPPEDFAYPKPADQYGYA 60 Query: 61 TRNKVFATPRDAALTVRYNTAQQALPTRKWGAFAPGTGVGGSGLHWTAVLIRATPTDLKL 120 TRNKVFATPRDAALTVRYNTAQQALPTRKWGAFAPGTGVGGSGLHWTAVLIRATPTDLKL Sbjct: 61 TRNKVFATPRDAALTVRYNTAQQALPTRKWGAFAPGTGVGGSGLHWTAVLIRATPTDLKL 120 Query: 121 KTYADQAYKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCGLSGTTGNLRGQIQPGGDPF 180 KTYADQAYKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCGLSGTTGNLRGQIQPGGDPF Sbjct: 121 KTYADQAYKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCGLSGTTGNLRGQIQPGGDPF 180 Query: 181 EGPRSNPYPLPALHDTYNNTLFADVARKLGYHPFPNPSANVSQAWTNPYGAQIAPCNYCG 240 EGPRSNPYPLPALHDTYNNTLFADVARKLGYHPFPNPSANVSQAWTNPYGAQIAPCNYCG Sbjct: 181 EGPRSNPYPLPALHDTYNNTLFADVARKLGYHPFPNPSANVSQAWTNPYGAQIAPCNYCG 240 Query: 241 FCSKYPCLNYSKASPQTTILDVVKRLPNFDYKVNANVIRVDLHADKKTARGVTYVDENMK 300 FCSKYPCLNYSKASPQTTILDVVKRLPNFDYKVNANVIRVDLHADKKTARGVTYVDENMK Sbjct: 241 FCSKYPCLNYSKASPQTTILDVVKRLPNFDYKVNANVIRVDLHADKKTARGVTYVDENMK 300 Query: 301 EVFQPAKIVILSSFQFANVRLMLLSGIGKPYDPITETGVIGRNYAFLSNGGATLFFKDKH 360 EVFQPAKIVILSSFQFANVRLMLLSGIGKPYDPITETGVIGRNYAFLSNGGATLFFKDKH Sbjct: 301 EVFQPAKIVILSSFQFANVRLMLLSGIGKPYDPITETGVIGRNYAFLSNGGATLFFKDKH 360 Query: 361 FNFFATAGATGEMFNDISPGNFDDGVKLGFIGGAKIHSSQASGAPIGTALPPGTPSWGRG 420 FN FATAGATGEMFNDISPGNFDDGVKLGFIGGAKIHSSQASGAPIGTALPPGTPSWGRG Sbjct: 361 FNSFATAGATGEMFNDISPGNFDDGVKLGFIGGAKIHSSQASGAPIGTALPPGTPSWGRG 420 Query: 421 WKEGMIDWYDHSMKISITTTCMSYRGHFLDLDPNYKDPWGQPLLRMTFDWRQNELKLQRY 480 WKEGMIDWYDHSMKISITTTCMSYRGHFLDLDPNYKDPWGQPLLRMTFDWRQNELKLQRY Sbjct: 421 WKEGMIDWYDHSMKISITTTCMSYRGHFLDLDPNYKDPWGQPLLRMTFDWRQNELKLQRY 480 Query: 481 LRDIVLKISKELNPDHMSESFLALDSHWDITKYVSTHNVGGAIMGDSPRDSALNKYLQSW 540 LRDIVLKISKELNPDHMSESFLALDSHWDITKYVSTHNVGGAIMGDSPRDSALNKYLQSW Sbjct: 481 LRDIVLKISKELNPDHMSESFLALDSHWDITKYVSTHNVGGAIMGDSPRDSALNKYLQSW 540 Query: 541 DVHNVFVPGGNAFPQNFQANPTATIGAITLMAAHAIKTQYLKNPGPLVKV 590 DVHNVFVPGGNAFPQNFQANPTATIGAITLMAAHAIKTQYLKNPGPLV+ Sbjct: 541 DVHNVFVPGGNAFPQNFQANPTATIGAITLMAAHAIKTQYLKNPGPLVQA 590 >gi|300312639|ref|YP_003776731.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 572 bits (1473), Expect = e-164, Method: Compositional matrix adjust. Identities = 283/591 (47%), Positives = 379/591 (64%), Gaps = 6/591 (1%) Query: 4 ITNDDVDVVIVGLGWAGSLMAIELAQAGLKVRALERGPDRPPE-DFAYPKPADQYGYATR 62 I D VDVV+VGLGW GS+M +EL +AGL V ALERG R D YPK D+ Y+ R Sbjct: 3 IKKDKVDVVMVGLGWTGSIMGMELTEAGLSVLALERGDMRDTNTDTPYPKAVDELKYSVR 62 Query: 63 NKVFATPRDAALTVRYNTAQQALPTRKWGAFAPGTGVGGSGLHWTAVLIRATPTDLKLKT 122 +F +T+R+ + A+P R+ G+F G GVGG+G HW ++ R P +L+++T Sbjct: 63 GALFQDLSRETVTIRHTLSDVAVPYRQHGSFLLGDGVGGAGFHWNGMMYRNLPEELEMRT 122 Query: 123 YADQAYKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCGLSGTTGNLRGQIQPGGDPFEG 182 + +Q Y + +M I+D T+ E+EP YDF +KV +SG GNL+GQI PGG+P EG Sbjct: 123 HHEQKYGKKFIPEDMTIQDHGVTYKELEPFYDFSEKVMAVSGKAGNLKGQIVPGGNPLEG 182 Query: 183 PRSNPYPLPALHDTYNNTLFADVARKLGYHPFPNPSANVSQAWTNPYGAQIAPCNYCGFC 242 RS+ +P P L Y+ +L R +GYHP+P P+AN SQ +TNPYG +I PCNYCGFC Sbjct: 183 ERSDEFPTPPLQYQYSASLVEKAMRDVGYHPYPAPAANASQPFTNPYGVRIGPCNYCGFC 242 Query: 243 SKYPCLNYSKASPQTTILDVVKRLPNFDYKVNANVIRVDLHADKKTARGVTYVDENMKEV 302 Y C YSKASPQTTIL V+ + NF+ + A VI+V+L A K A GVTY+D +EV Sbjct: 243 ENYGCYMYSKASPQTTILPVLLKRKNFEVRTKAQVIKVNLDASGKRATGVTYIDAQGREV 302 Query: 303 FQPAKIVILSSFQFANVRLMLLSGIGKPYDPITETGVIGRNYAFLSNGGATLFF-KDKHF 361 QPA +V+L++FQ NVRL+LLSGIGKPYDP+T GV+G+N+A+ NGG + K F Sbjct: 303 EQPADLVLLTAFQTHNVRLLLLSGIGKPYDPVTGEGVVGKNFAYQYNGGINVIMPKGTQF 362 Query: 362 NFFATAGATGEMFNDISPGNFDDGVKLGFIGGAKIHSSQASGAPI---GTALPPGTPSWG 418 N F GA G +D++ FD G LGFIGGA I + G PI GT PSWG Sbjct: 363 NPFIGTGAGGVGMDDLNADQFDHG-PLGFIGGASIRHVRYGGRPIKQAGTMPGEKVPSWG 421 Query: 419 RGWKEGMIDWYDHSMKISITTTCMSYRGHFLDLDPNYKDPWGQPLLRMTFDWRQNELKLQ 478 WK + D Y ++ I ++ + M+YR +L LDP Y+D GQPLLR+T +W++NE K+ Sbjct: 422 SEWKASVQDAYQRTLTIGMSGSVMAYRDCYLSLDPTYRDANGQPLLRLTLNWKENEGKML 481 Query: 479 RYLRDIVLKISKELNPDHMSESFLALDSHWDITKYVSTHNVGGAIMGDSPRDSALNKYLQ 538 Y+ + K+ + +NP+ + ++ S WD Y STH GGAIMG +P++S +NKYLQ Sbjct: 482 TYIASQMEKVGQAMNPEKVVKAVRKTGSSWDTRVYQSTHLTGGAIMGTTPKNSVVNKYLQ 541 Query: 539 SWDVHNVFVPGGNAFPQNFQANPTATIGAITLMAAHAIKTQYLKNPGPLVK 589 SWDV NVFV G AFPQN NPT + A+ +A AI+ QYLKNPGPLV+ Sbjct: 542 SWDVPNVFVYGACAFPQNMGYNPTGLVTALAYHSAKAIREQYLKNPGPLVQ 592 >gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 508 bits (1307), Expect = e-145, Method: Compositional matrix adjust. Identities = 255/585 (43%), Positives = 358/585 (61%), Gaps = 5/585 (0%) Query: 9 VDVVIVGLGWAGSLMAIELAQAGLKVRALERGPDRPP-EDFAYPKPADQYGYATRNKVFA 67 VDVVIVG GW G++MA E+ +AG +V ALERG R D AYPK ++ Y R K+F Sbjct: 9 VDVVIVGFGWTGAIMAKEMTEAGQRVVALERGVYRDTYPDGAYPKTINELEYQQRFKLFQ 68 Query: 68 TPRDAALTVRYNTAQQALPTRKWGAFAPGTGVGGSGLHWTAVLIRATPTDLKLKTYADQA 127 ++ T R T A+P R+ F PG GVGG+GLHW+ R P +L+++++ ++ Sbjct: 69 NLNKSSFTFRRKTGDSAIPYRQIAMFKPGEGVGGAGLHWSGCHWRILPEELRMRSHYEER 128 Query: 128 YKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCGLSGTTGNLRGQI-QPGGDPFEGPRSN 186 Y + + +M ++D+ ++ E+EP++DF +K+ G SGT + G++ G+PFE RS+ Sbjct: 129 YGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYRVGGKVVDESGNPFEADRSD 188 Query: 187 PYPLPALHDTYNNTLFADVARKLGYHPFPNPSANVSQAWTNPYGAQIAPCNYCGFCSKYP 246 +PLPA + Y LF A++ G HPF PSAN + + N YG Q+ PC +CGFCS Y Sbjct: 189 NFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYVNQYGCQMGPCTFCGFCSGYA 248 Query: 247 CLNYSKASPQTTILDVVKRLPNFDYKVNANVIRVDLHADKKTARGVTYVDENMKEVFQPA 306 C NYSKASP I+ +++ F+ + + NV+R++L + +K A GVTYVD N VFQPA Sbjct: 249 CYNYSKASPNVNIMPALRQSNLFELRSSCNVLRIELDSSRKKATGVTYVDINGDTVFQPA 308 Query: 307 KIVILSSFQFANVRLMLLSGIGKPYDPITETGVIGRNYAFLSNGGATLFFK-DKHFNFFA 365 IVI S+F + N RL LLSGIGKPYDP++ TG +GRN AF FF+ K+ N F Sbjct: 309 SIVIASTFAYNNARLFLLSGIGKPYDPVSNTGAVGRNIAFQMMSTINAFFEPGKNINGFI 368 Query: 366 TAGATGEMFNDISPGNFDDGVKLGFIGGAKIHSSQASGAPI-GTALPPGTPSWGRGWKEG 424 AG G +D + + D G LGF+GG+ I + + PI G A+P GTP WGR WK+ Sbjct: 369 GAGGNGVAVDDFNGDHMDHG-PLGFVGGSPIWCNPSGSKPISGIAVPSGTPRWGRAWKQA 427 Query: 425 MIDWYDHSMKISITTTCMSYRGHFLDLDPNYKDPWGQPLLRMTFDWRQNELKLQRYLRDI 484 + D Y ++M + M YR ++DLDPNYKD +GQPLLR TFDW+ N++++ RY+ D Sbjct: 428 VKDSYLNTMSFDVHGANMVYRDVYVDLDPNYKDAFGQPLLRFTFDWKDNDIRMSRYVTDQ 487 Query: 485 VLKISKELNPDHMSESFLALDSHWDITKYVSTHNVGGAIMGDSPRDSALNKYLQSWDVHN 544 +KI+ EL + + + D+ Y +TH GG MG P S +N+YLQSWDVHN Sbjct: 488 AVKIAHELKAREIKVTVKDFGAAPDLRSYQTTHWAGGVAMGTDPSTSVVNRYLQSWDVHN 547 Query: 545 VFVPGGNAFPQNFQANPTATIGAITLMAAHAIKTQYLKNPGPLVK 589 VF G F Q NPT A+T +A AI+ QYL +P PLV+ Sbjct: 548 VFAVGSGVFAQGIGYNPTGAAVALTYWSARAIREQYLNDPRPLVQ 592 >gi|300313052|ref|YP_003777144.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 543 Score = 82.8 bits (203), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 118/501 (23%), Positives = 191/501 (38%), Gaps = 77/501 (15%) Query: 99 VGGSGLHWTAVLIRATPTDLKLKTYADQAYKHSNLDPEMRIKDFPFTWNEIEPHYDFFDK 158 GGS HW R TP D++L + +D+ F ++ +EP+Y + Sbjct: 102 AGGSTWHWAGTCWRLTPEDMRLHSLYGVG------------RDWAFGYDVLEPYYTRVEH 149 Query: 159 VCGLSGTTGNLRGQIQPGGDPFEGP--RSNPYPLPALHDTYNNTLFADVARKLGYHPFPN 216 G+ G P + P RS PYP+PAL + F +V + LGY P Sbjct: 150 QLGICG----------PSDPALQWPPQRSAPYPMPALPFSPGEQRFTEVVQALGYRNIPT 199 Query: 217 PSANVSQAWTNPYGAQIAPCNYCGFCSKYPCLNYSKASPQTTILDVVKRLPNFDYK---- 272 A S PY + A C+ C+ P D L + K Sbjct: 200 AQARNSGM---PYDDRPA------CCANNNCV---PVCPVGAKYDAATALARLEAKGATI 247 Query: 273 -VNANVIRVDLHADKKTARGVTYVDENMKEVFQPAKIVILSSFQFANVRLMLLSGIGK-P 330 +A V RVD A+ + V Y D N KI +L+ +L+L+S + P Sbjct: 248 LTDAVVYRVDTDANDRV-EAVNYFDANKLSHRITGKIFVLACNGIETPKLLLMSANSRNP 306 Query: 331 YDPITETGVIGRNYAFLSNGGATLFFKDKHFNFFATAGATGEMFNDISPGNFDDGVKLGF 390 + + +GRN TL + +++ +G M + S G F + Sbjct: 307 HGLANRSDQVGRNMMDHPQLTMTLTLSEPYWSGVGPVVNSGIM--ETSQGAF----RSEH 360 Query: 391 IGGAKIHSSQASGAPIGTALPPGTPSWGRGWKEGMI-DWYDHSMKISITTTCMSYRGHFL 449 G +++ A + A K+G++ D ++ T H Sbjct: 361 AGAYFRYNNFARNRFVTFA----------ALKKGLVGQALDEEIRRMTACTADIVLAH-- 408 Query: 450 DLDPNYKDPWGQPLLRMTFDWRQNELKLQR----YLRDIVLKISKELNP----DHMSESF 501 ++ P+ ++ R+T +++ L L R Y + S EL ++E+ Sbjct: 409 EVLPDAEN-------RLTLSSKKDWLGLPRPAIHYDVGDYTRRSAELYSLPIGRKIAEAM 461 Query: 502 LALDSHWDITKYVSTHNVGGAIMGDSPRDSALNKYLQSWDVHNVFVPGGNAFPQNFQANP 561 A D S H +GG IMG P +S ++ ++ D N+++PGG A P N Sbjct: 462 GATDIKVSPKFAQSKHIMGGTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNS 521 Query: 562 TATIGAITLMAAHAIKTQYLK 582 T ++ A+ L AA AI Q K Sbjct: 522 TISMAALGLKAADAILRQLQK 542 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,051,155 Number of extensions: 93592 Number of successful extensions: 245 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 236 Number of HSP's successfully gapped: 4 Length of query: 590 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 499 Effective length of database: 1,188,985 Effective search space: 593303515 Effective search space used: 593303515 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)