BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0252.p (304 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313833|ref|YP_003777925.1| sugar ABC transporter permease ... 602 e-174 gi|300312388|ref|YP_003776480.1| sugar ABC transporter permease ... 103 2e-23 gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease ... 88 1e-18 gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC tra... 85 9e-18 gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease ... 79 3e-16 >gi|300313833|ref|YP_003777925.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 304 Score = 602 bits (1553), Expect = e-174, Method: Compositional matrix adjust. Identities = 304/304 (100%), Positives = 304/304 (100%) Query: 1 MKTIRAWQLAVLLAPAVLIYALFSALPLLDTLRLGFYTANDAGVRSFVGLDNYRTILFDP 60 MKTIRAWQLAVLLAPAVLIYALFSALPLLDTLRLGFYTANDAGVRSFVGLDNYRTILFDP Sbjct: 1 MKTIRAWQLAVLLAPAVLIYALFSALPLLDTLRLGFYTANDAGVRSFVGLDNYRTILFDP 60 Query: 61 DWSAAFWNAMWNNVKFFCIHMALQNPIGLLLATLFSLKGLRGARTYRTLIFLPTLLSVVI 120 DWSAAFWNAMWNNVKFFCIHMALQNPIGLLLATLFSLKGLRGARTYRTLIFLPTLLSVVI Sbjct: 61 DWSAAFWNAMWNNVKFFCIHMALQNPIGLLLATLFSLKGLRGARTYRTLIFLPTLLSVVI 120 Query: 121 IGFIWQLILSPLWGVGEKLLGTVGLADWFAPWLGQESTALLTLSLISVWQYVGIPMMLIY 180 IGFIWQLILSPLWGVGEKLLGTVGLADWFAPWLGQESTALLTLSLISVWQYVGIPMMLIY Sbjct: 121 IGFIWQLILSPLWGVGEKLLGTVGLADWFAPWLGQESTALLTLSLISVWQYVGIPMMLIY 180 Query: 181 AALLAVPEEVLEAAHAEGASAMRIFWQIKLPLIYPTLGLVTILTFVANFNAFDLIYSVKG 240 AALLAVPEEVLEAAHAEGASAMRIFWQIKLPLIYPTLGLVTILTFVANFNAFDLIYSVKG Sbjct: 181 AALLAVPEEVLEAAHAEGASAMRIFWQIKLPLIYPTLGLVTILTFVANFNAFDLIYSVKG 240 Query: 241 ALAGPNYASDLLGTYFYRTFFGYQAQIGSPTMGAAVATLMFLVILGGVGAYFVLVQRRLT 300 ALAGPNYASDLLGTYFYRTFFGYQAQIGSPTMGAAVATLMFLVILGGVGAYFVLVQRRLT Sbjct: 241 ALAGPNYASDLLGTYFYRTFFGYQAQIGSPTMGAAVATLMFLVILGGVGAYFVLVQRRLT 300 Query: 301 RYAM 304 RYAM Sbjct: 301 RYAM 304 >gi|300312388|ref|YP_003776480.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 291 Score = 103 bits (256), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 88/256 (34%), Positives = 133/256 (51%), Gaps = 14/256 (5%) Query: 11 VLLAPAVLIYALFSALPLLDTLRLGFYTANDAGVRS--FVGLDNYRTILFDPDWSAAFWN 68 +LL+PA+++ F+ P L TL F++ GVR FVGL+NYR + DP FW Sbjct: 12 LLLSPAMVLLIAFTHYPALATLWHSFFS-TPKGVRPSVFVGLENYRLMADDP----VFWQ 66 Query: 69 AMWNNVKFFCIHMALQNPIGLLLATLFSLKGLRGARTYRTLIFLPTLLSVVIIGFIWQLI 128 A+ NN+ F + L + +L+A L+ + L G R F PT+L ++ + IW Sbjct: 67 ALCNNLWFALGTIPLAIALAILMA-LWVNEKLAGRGVLRLAYFTPTVLPMIAVANIWLFF 125 Query: 129 LSPLWGVGEKLLGTVGLADWFAPWLGQESTALLTLSLISVWQYVGIPMMLIYAALLAVPE 188 +P +G+ E++ G +GL WLG T L L +++VW+ G M+ AAL + Sbjct: 126 YTPQYGLLEQITGALGLPS--HNWLGNPQTVLGALMVVAVWKEAGFFMIFYLAALQQISP 183 Query: 189 EVLEAAHAEGASAMRIFWQIKLPLIYPTLGLVTILTFVANFNAFD-LIYSVKGALAGPNY 247 + EAA EGAS + FW+++ PL+ PT V I + F D ++ KG GP+ Sbjct: 184 VLAEAAALEGASRWQYFWRVQFPLLMPTTLFVLINALINAFRLVDHIVVMTKG---GPDN 240 Query: 248 ASDLLGTYFYRTFFGY 263 AS LL Y Y F + Sbjct: 241 ASSLLLYYIYEVGFAF 256 >gi|300309613|ref|YP_003773705.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 311 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 82/289 (28%), Positives = 133/289 (46%), Gaps = 22/289 (7%) Query: 13 LAPAVLIYALFSALPLLDTLRLGFYTANDAGVRSFVGLDNYRTILFDPDWSAAFWNAMWN 72 LAPA ++ ++ P+L T+ L F++ + FVGL NY + P F+ A+ N Sbjct: 42 LAPACVMTLVYVLYPILYTIYLSFFSWDGMTEPRFVGLANYVELFHAP----TFYTALKN 97 Query: 73 NVKFFCIHMALQNPIGLLLATLFSLKGLRGARTYRTLIFLPTLLSVVIIGFIWQLILSPL 132 NV + + + L P+GL +A + K + G R ++L F P +LS V++G ++ P Sbjct: 98 NVLWLLMFL-LAPPMGLAIALYLNQKVI-GMRVVKSLFFAPFVLSGVVVGLMYSWFYDPS 155 Query: 133 WGVGEKLLGTVGLADWFAPWLGQESTALLTLSLISVWQYVGIPMMLIYAALLAVPEEVLE 192 +G+ L G P LG A + ++W M+L L A+ + +E Sbjct: 156 FGLLSLLFGQG------VPVLGDARYATFGIIFAALWPQTAYCMILFLTGLTALNHDQVE 209 Query: 193 AAHAEGASAMRIFWQIKLPLIYPTLGLVTILTFVANFNAFDLIYSVKGALAGPNYASDLL 252 AA EGA + + LP + T + +++ + +FDLI + GP +S +L Sbjct: 210 AARMEGAKGWTMLRYVILPQLRSTTFMAFVVSIIGALRSFDLISVMTS--GGPYESSTVL 267 Query: 253 GTYFYRTFFGYQAQIGSPTMGAAVATLMFLVILGGVGAYFVLVQRRLTR 301 Y Y Y Q S AAVA +F ++L Y V RR+ R Sbjct: 268 AYYAYDQAIKYYRQGYS----AAVAVTLFAIML----VYIVYQLRRMLR 308 >gi|300313088|ref|YP_003777180.1| sn-glycerol-3-phosphate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 294 Score = 84.7 bits (208), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 71/254 (27%), Positives = 125/254 (49%), Gaps = 24/254 (9%) Query: 7 WQLAVLLAPAVLIYALFSALPLLDTLRLGFYTAND-AGVRSFVGLDNYRTILFDPDWSAA 65 W +L+AP ++I LF P + L + G FV DN++T+ DP + + Sbjct: 11 WLPYLLVAPQIIITLLFFFWPAVQALYQSMLLQDAFGGYSEFVWFDNFKTLFGDPTYLES 70 Query: 66 FWN-AMWNN-VKFFCIHMALQNPIGLLLATLFSLKGLRGARTYRTLIFLPTLLSVVIIGF 123 F A+++ V FF + ++L L +F+ + RGA Y+T + P +S V++G Sbjct: 71 FRTTAIFSALVAFFGLALSL-------LLAVFADRVRRGASIYKTFLIWPYAVSPVVVGV 123 Query: 124 IWQLILSPLWGVGEKLLGTVGLADWFAPW--LGQESTALLTLSLISVWQYVGIPMMLIYA 181 +W +LSP G+ +L +GL PW + + A++ + + ++W+ + + A Sbjct: 124 LWMFLLSPSLGILSHVLSWLGL-----PWDYMINGTHAMILIVIAAIWKQISYNFLFFLA 178 Query: 182 ALLAVPEEVLEAAHAEGASAMRIFWQIKLPLIYPTLGLVTILTFVAN--FNAFDLIYSVK 239 L ++P+ ++EAA +GA ++ F+ I PLI PT T FV N + FD V Sbjct: 179 GLQSIPKSLIEAAAIDGAGPVKRFFTIVFPLISPT----TFFLFVVNVVYAFFDTFVIVD 234 Query: 240 GAL-AGPNYASDLL 252 GP +++L Sbjct: 235 ATTHGGPGKDTEIL 248 >gi|300309782|ref|YP_003773874.1| sugar ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 324 Score = 79.3 bits (194), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 81/295 (27%), Positives = 140/295 (47%), Gaps = 32/295 (10%) Query: 15 PAVLIYALFSALPLLDTL-----RLGFYTANDAGVRSFVGLDNYRTILFDPDWSAAFWNA 69 P+VLI + +PL T+ R + G FVGL+NY +L DP AFW + Sbjct: 46 PSVLILLAWMIVPLAMTIYFSVNRYNLMAPDQTG---FVGLENYAFLLQDP----AFWPS 98 Query: 70 MWNNVKFFCIHMALQNPIGLLLATLFSLKGLRGARTYRTLIFLPTLLSVVIIGFIWQ-LI 128 + N +A+ G LLA LF + RG R L+ P + + IW+ +I Sbjct: 99 IGNTALLIGSVLAISVIGGTLLAVLFD-QPFRGRGIARLLVIGPFFVMPTVAALIWKNMI 157 Query: 129 LSPLWGVGEKLLGTVGLA--DWFAPWLGQESTALLTLSLISVWQYVGIPMMLIYAALLAV 186 + P++G+ ++ +GL DW A + +L++ +I WQ++ +++ AL ++ Sbjct: 158 MHPVYGILAYVMRHLGLEPIDWLAEY------PMLSVIIIVAWQWIPFAFLILLTALQSL 211 Query: 187 PEEVLEAAHAEGASAMRIFWQIKLPLIYPTLGLVTILTFVANFNAFDLIYSVKGALAGPN 246 + EAA +GA +RIF+ I LP + + +V ++ + + F I++ GP Sbjct: 212 DTDQKEAAQLDGAGPVRIFFYIVLPHLKRAITVVIMIETIFLLSVFAEIFTTTA--GGPG 269 Query: 247 YASDLLGTYFYRTFFGYQAQIGSPTMGAAVATLMFLVILGGVGAYFVLVQRRLTR 301 A+ L TF Y + +G A A +F V+L + ++F++ R L R Sbjct: 270 TATTNL------TFLVYSIGLQQFDIGIASAGGIFAVVLANIVSFFLV--RMLAR 316 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,009,467 Number of extensions: 44651 Number of successful extensions: 244 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 227 Number of HSP's successfully gapped: 11 Length of query: 304 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 219 Effective length of database: 1,217,395 Effective search space: 266609505 Effective search space used: 266609505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)