BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0247.p (491 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbas... 963 0.0 gi|300313666|ref|YP_003777758.1| two component sensor histidine ... 194 2e-50 gi|300310848|ref|YP_003774940.1| two component sensor histidine ... 187 2e-48 gi|300311468|ref|YP_003775560.1| oxidative stress resistance two... 125 8e-30 gi|300313895|ref|YP_003777987.1| two-component system sensor his... 97 3e-21 >gi|300313829|ref|YP_003777921.1| sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 478 Score = 963 bits (2490), Expect = 0.0, Method: Compositional matrix adjust. Identities = 477/478 (99%), Positives = 478/478 (100%) Query: 14 LPDSLLGRLSVVMIFGVLVTQVAGGLIWAVQLRSKSEAETRTAAQHLAHSAASAIRYFMT 73 +PDSLLGRLSVVMIFGVLVTQVAGGLIWAVQLRSKSEAETRTAAQHLAHSAASAIRYFMT Sbjct: 1 MPDSLLGRLSVVMIFGVLVTQVAGGLIWAVQLRSKSEAETRTAAQHLAHSAASAIRYFMT 60 Query: 74 LPPNYRPLMIQQLREMGGTRFFINANSSAVAVKSIGDSRLAKLAIDVAAQTLREDLPFLP 133 LPPNYRPLMIQQLREMGGTRFFINANSSAVAVKSIGDSRLAKLAIDVAAQTLREDLPFLP Sbjct: 61 LPPNYRPLMIQQLREMGGTRFFINANSSAVAVKSIGDSRLAKLAIDVAAQTLREDLPFLP 120 Query: 134 EARLAFAWPDGLPVASDGLTVNDLPDNWVQHILMTKPNSAPVLVIQMQVESGRWIYLAAL 193 EARLAFAWPDGLPVASDGLTVNDLPDNWVQHILMTKPNSAPVLVIQMQVESGRWIYLAAL Sbjct: 121 EARLAFAWPDGLPVASDGLTVNDLPDNWVQHILMTKPNSAPVLVIQMQVESGRWIYLAAL 180 Query: 194 MPNPYFLDSDNPLWLDRLVLQGLSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVP 253 MPNPYFLDSDNPLWLDRLVLQGLSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVP Sbjct: 181 MPNPYFLDSDNPLWLDRLVLQGLSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVP 240 Query: 254 SLPETGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDA 313 SLPETGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDA Sbjct: 241 SLPETGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDA 300 Query: 314 MRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDARLAGHEIAFTPS 373 MRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDARLAGHEIAFTPS Sbjct: 301 MRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDARLAGHEIAFTPS 360 Query: 374 GINVIAKPLALKRAIGNLFDNALYYGQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAM 433 GINVIAKPLALKRAIGNLFDNALYYGQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAM Sbjct: 361 GINVIAKPLALKRAIGNLFDNALYYGQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAM 420 Query: 434 GTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPAR 491 GTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPAR Sbjct: 421 GTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPAR 478 >gi|300313666|ref|YP_003777758.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 479 Score = 194 bits (492), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 141/488 (28%), Positives = 234/488 (47%), Gaps = 37/488 (7%) Query: 15 PDSLLGRLSVVMIFGVLVTQVAGGLIWA-VQLRSKSEAETRTAAQHLAHSAASAIRYFMT 73 P +L GRL+++++ G+L Q+ G IW ++ + E TR AA HLA A+ Sbjct: 15 PQTLSGRLALILVAGMLAAQMLTGTIWYDMRYGTAMEMPTRLAAAHLA----DAVLILDD 70 Query: 74 LPPNYRPLMIQQLREMGGTRFFIN----ANSSAVAVKSIG-----DSRLAKLAIDVAAQT 124 P R + L +F ++ + + +AV + + + +D+ Sbjct: 71 APATQRQALAHSLTR---AQFVLSLPDVVDRADLAVPGLAPASSPTTHADQGVVDLFGTV 127 Query: 125 LREDLPFLPEARLAFAWPDGLPVASDG---LTVNDLPDNWVQHILMTKPNSAPVLVIQMQ 181 L++ P L D A L ++ P + ++ K S +Q Sbjct: 128 LQQHAGPRPWKMLQLGLDDQHANAESSWWTLLRDEFPHGRFRVLVTLKDGS----WLQAD 183 Query: 182 VESGRWIYLAALMPNPYFLDSDNPLWLDRLVLQGLSLAAILLLTILVVRWTTRPLAALAD 241 +E G+ A + P+ D + + L + A++L+ + VR +PL +A+ Sbjct: 184 IEEGQ----AGMSTAPHSAAFDY-----FMRIYALRIFAVVLIAWMAVRLAMKPLQVMAE 234 Query: 242 AAEAFGKGETVPSLPETGSREYVKTARAFSGMRERIKRYLEDRERLFVSISHDLRTPITR 301 AAE G L G E A+AF+ M+ RI + +R R ++SHDLR+PITR Sbjct: 235 AAEKLGGNIRSAPLATDGPLEVSTAAQAFNAMQRRIIDNIAERTRFLAAVSHDLRSPITR 294 Query: 302 LKLRTELMDDDAMRTDFHDDLDELDMMVKGALQSVKDSDIHENRTEVRLDALVLRMIRDA 361 +KLRTE++ D+ + F DLDE+D ++ L V+D D+ E + LD+L+ + D Sbjct: 295 MKLRTEMLPDERGKEKFRKDLDEMDALISATLNVVQDIDVQERPQLIDLDSLLESLRTDQ 354 Query: 362 RLAGHEIAFTPSGINVIAKPLALKRAIGNLFDNALYYGQKVEISIRPSPGAEGDLVEIEI 421 G E+ + + P +L+R + NL +NA+ YGQ+ + ++ + D V I + Sbjct: 355 AEVGGEVTLSGHAAPLQGYPRSLRRCLQNLVENAVRYGQRARVQLQD----DADQVCIRV 410 Query: 422 RDHGPGVPEDAMGTLFQPYVRLEHGRAQNSGGMGLGLGISRNIVQAHGGELLLRNHPEGG 481 D GPG+PE + + +P+ RLE R +GG GLGL I+ +VQAH G + L N P GG Sbjct: 411 DDDGPGIPEALLERVQEPFYRLERSRNAGTGGFGLGLSIAATVVQAHRGVMKLVNRPGGG 470 Query: 482 LMATIILP 489 L I++P Sbjct: 471 LRVEILIP 478 >gi|300310848|ref|YP_003774940.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 465 Score = 187 bits (474), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 108/275 (39%), Positives = 156/275 (56%), Gaps = 5/275 (1%) Query: 216 LSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVPSLPETGSREYVKTARAFSGMRE 275 L L I +L LV R TRP+ LA AA G L ETG E + A AF+ M+ Sbjct: 187 LFLVLIAMLAWLVARMATRPIRQLAQAASRLGSDIDHAPLAETGPTEIRQAAHAFNAMQA 246 Query: 276 RIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDAMRTDFHDDLDELDMMVKGALQS 335 RI+R ++ R + +I+HDL+TP+TR++LR E + D ++ DDL + MV+ L Sbjct: 247 RIRRQIQHRTHMLAAITHDLQTPLTRMRLRLEKVSDPGLQQKLIDDLGVMHGMVREGLDL 306 Query: 336 VKDSDIHENRTEVRLDALVLRMIRDARLAGHEIAFTPSG-INVIAKPLALKRAIGNLFDN 394 + D E + +D+L+ + DA AG ++A S V+A+P AL+R + NL DN Sbjct: 307 ARSMDSSEKIQALDIDSLLDSVCADAADAGQDVALQGSTRAFVMAQPGALRRCLTNLIDN 366 Query: 395 ALYYGQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAMGTLFQPYVRLEHGRAQNSGGM 454 A YG+ +SI E + I IRD GPG+PE + +F+P+ RLE R++++GG Sbjct: 367 ACKYGKSARVSI----ALEQQKIAIRIRDQGPGIPESELEAVFEPFYRLEASRSRDTGGT 422 Query: 455 GLGLGISRNIVQAHGGELLLRNHPEGGLMATIILP 489 GLGL I+ NI + H G+L LRN EGGL + LP Sbjct: 423 GLGLTIALNIAENHHGQLRLRNLAEGGLEVLLELP 457 >gi|300311468|ref|YP_003775560.1| oxidative stress resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 455 Score = 125 bits (314), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 101/284 (35%), Positives = 136/284 (47%), Gaps = 18/284 (6%) Query: 216 LSLAAILLLTILVVRWTTRPLAALADAAEAFGKGETVPSLPETGSREYVKTARAFSGMRE 275 LSL + ++ L+ +PLA L AA A KG LPE+G E + R+F+ M Sbjct: 172 LSLIGAVFISTLI----NQPLARLTAAARAIAKGRQPEPLPESGPTEIEEANRSFNQMVA 227 Query: 276 RIKRYLEDRERLFVSISHDLRTPITRLKLRTELMD-DDAMRTDFHDDLDELDMMVKGALQ 334 + R DR + ISHDLRTP+ R++L E+ + D R H DL ++D ++ L Sbjct: 228 DLNRIESDRALILAGISHDLRTPLARMQLEVEMANLSDEARDGMHSDLAQMDSIIGQFLD 287 Query: 335 SVKDSDIHENRTEVRLDALVLRMIRDARLAGHEI--AFTPSGINVIAKPLALKRAIGNLF 392 K D T D L +RL+ + TP V L R NL Sbjct: 288 YAKPFDTSSLETIDLADVLQDVAGNASRLSDIRVHTQITPD-TQVAGNGTELARVFSNLV 346 Query: 393 DNALYYGQK-----VEISIRPSPGAEGDLVEIEIRDHGPGVPEDAMGTLFQPYVRLEHGR 447 +NA YG+ EI IR EG V +E+ DHGPGVP + L +P+ RL+ R Sbjct: 347 ENARRYGKTEGTDCAEIDIRSR--IEGHQVVVEVADHGPGVPVNECERLLRPFTRLDTAR 404 Query: 448 AQNSGGMGLGLGISRNIVQAHGGELLLRNHPE--GGLMATIILP 489 Q + G GLGL I IV H G+LLL+ H E GL+ I LP Sbjct: 405 GQ-ANGSGLGLAIVNRIVLKHNGKLLLKGHEETRSGLLVQITLP 447 >gi|300313895|ref|YP_003777987.1| two-component system sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 97.1 bits (240), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 81/296 (27%), Positives = 138/296 (46%), Gaps = 25/296 (8%) Query: 216 LSLAAILLLTILVVRWT---TRPLAALADAAEAFGKGETVPSLPETGSREYVKTARAFSG 272 +++ A++L LVVR + TRPL +A A G+ + E A+ F+ Sbjct: 177 IAVVAVMLAAFLVVRISGVLTRPLTRVARATHRLASGDYAIRVEVESGDEVGMLAQDFNQ 236 Query: 273 MRERIKRYLEDRERLFVSISHDLRTPITRLKLRTELMDDDAMRTD------FHDDLDELD 326 + ++R + R +SH+LRTP+ L + E ++D ++ D +++ L+ Sbjct: 237 LAMTLERNEKLRREFMADVSHELRTPLAILNGQLEALEDGVLQPDAQTLRSLKSEVEHLN 296 Query: 327 MMVKGALQSVKDSD---IHENRTEVRL-----DALVLRMIRDARLAGHEIAFTPSGINVI 378 +V G L + +D + R ++ L D+ L R ++ P+ + V Sbjct: 297 KLV-GDLYDLSLADAGALTYRRADIDLAPLLRDSAALYSDRFSQRGMRLSCELPASMPVH 355 Query: 379 AKPLALKRAIGNLFDNALYY---GQKVEISIRPSPGAEGDLVEIEIRDHGPGVPEDAMGT 435 L++ + NLF+N+L Y G ++++ + + D PGVP + M Sbjct: 356 GDAARLQQLLSNLFENSLRYTDAGGRLQVHAH----LQQQHWHLVFDDSEPGVPPEVMAR 411 Query: 436 LFQPYVRLEHGRAQNSGGMGLGLGISRNIVQAHGGELLLRNHPEGGLMATIILPAR 491 LF+ + R+E R + SGG GLGL I R IV AH G + P GGL ++LPAR Sbjct: 412 LFERFFRVEASRNRASGGAGLGLAICRRIVDAHQGTIGASASPLGGLRVVVVLPAR 467 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,421,833 Number of extensions: 58793 Number of successful extensions: 316 Number of sequences better than 1.0e-08: 34 Number of HSP's gapped: 286 Number of HSP's successfully gapped: 34 Length of query: 491 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 402 Effective length of database: 1,198,455 Effective search space: 481778910 Effective search space used: 481778910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)