BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0246.p (344 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 704 0.0 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 153 2e-38 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 124 1e-29 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 110 2e-25 gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 71 1e-13 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 704 bits (1818), Expect = 0.0, Method: Compositional matrix adjust. Identities = 344/344 (100%), Positives = 344/344 (100%) Query: 1 MSYIAEPSRYDGRMPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTH 60 MSYIAEPSRYDGRMPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTH Sbjct: 1 MSYIAEPSRYDGRMPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTH 60 Query: 61 FDLANNYGPPAGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIA 120 FDLANNYGPPAGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIA Sbjct: 61 FDLANNYGPPAGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIA 120 Query: 121 SCEQSLKRMGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKA 180 SCEQSLKRMGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKA Sbjct: 121 SCEQSLKRMGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKA 180 Query: 181 AAILKSEGVPLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVP 240 AAILKSEGVPLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVP Sbjct: 181 AAILKSEGVPLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVP 240 Query: 241 EGARVTRAPSLPQRMLSEDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGAR 300 EGARVTRAPSLPQRMLSEDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGAR Sbjct: 241 EGARVTRAPSLPQRMLSEDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGAR 300 Query: 301 TVEQLDDSLDAVNKLDFTQEELASIDKHATDGDVDLWKVSSSIT 344 TVEQLDDSLDAVNKLDFTQEELASIDKHATDGDVDLWKVSSSIT Sbjct: 301 TVEQLDDSLDAVNKLDFTQEELASIDKHATDGDVDLWKVSSSIT 344 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 153 bits (387), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 114/324 (35%), Positives = 169/324 (52%), Gaps = 15/324 (4%) Query: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPPAGS 73 M YRR G+S L + A+ LG D E+GR ++ A + GV D A+ Y G Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGR-IVASAREHGVNFIDTADVY--TKGK 57 Query: 74 AEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIASCEQSLKRMGLDY 133 +EE G+ +A R E V++SK G M P + +R ++ E L R+ DY Sbjct: 58 SEEIVGKLLAGQ----RHEWVLASKLGNAMSGKP-NESNYSRSWIVRETENILTRLDTDY 112 Query: 134 VDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEGVPL-L 192 +DI Y HR D LEE + AL L R GK G+S++ + + + VPL L Sbjct: 113 LDILYMHRDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPL 172 Query: 193 IHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVPEGARVTRAPSLP 252 + QP Y++LNR E +LPA G+G + +SP+A+G+LT KY G + A+ TRA Sbjct: 173 VCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPG-QKPAQDTRAGRGD 231 Query: 253 QRMLS----EDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGARTVEQLDDS 308 +RM+ E++L + L + RG L Q A AWVL ++ ++S + G RT+ Q++D Sbjct: 232 KRMMETEFREESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDY 291 Query: 309 LDAVNKLDFTQEELASIDKHATDG 332 DA +L + EE A +D T G Sbjct: 292 YDAA-ELKISAEEEALVDSLVTPG 314 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 100/321 (31%), Positives = 158/321 (49%), Gaps = 29/321 (9%) Query: 17 RRTGRSGLLLPAISLGLWQNFGGVDNFETGR------AVLRRAFDRGVTHFDLANNYGPP 70 RR G +G L+ AI LG G+ +F R A + RA + G+ D A+ YGP Sbjct: 18 RRLGSNGPLVSAIGLGCM----GMSDFYAQRDDAESLATIDRALELGINLLDTADMYGP- 72 Query: 71 AGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYG--GPSGARKHLIASCEQSLKR 128 + EE GR + RD+ I++K G + P G G G+ ++ + E SLKR Sbjct: 73 -YTNEELLGRAIKG----RRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKR 127 Query: 129 MGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKSEG 188 +G++ +D++Y HR+D TP+E TMG LA L + GK Y+G+S S AA + ++ Sbjct: 128 LGVETIDLYYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSEAS------AATLERAHR 181 Query: 189 V-PLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTS--KYLGGVPEGARV 245 V P+ Q YS+ R E +L A LG+G + +SPL +G LT + + E Sbjct: 182 VHPVTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFR 241 Query: 246 TRAPSLPQRMLSEDNLARIRALNEIASKRGQTLAQMAIAWVLRDARVTSALIGARTVEQL 305 P + NL + E+A + G T +Q+A+AWV+ + G + L Sbjct: 242 RSNPRFQGENFAR-NLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYL 300 Query: 306 DDSLDAVNKLDFTQEELASID 326 +D+ +V + E+L ++D Sbjct: 301 EDNAGSVG-VKLAPEDLQALD 320 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 110 bits (275), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 106/351 (30%), Positives = 163/351 (46%), Gaps = 51/351 (14%) Query: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPP--- 70 M YR GR+ L + I+LG +G ++ A L A +RG+ D+A Y P Sbjct: 1 MQYRPLGRTDLKVSLITLGT-MTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKA 59 Query: 71 --AGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGAR-----------KH 117 G E G W+ K RD+L+I++K GP P R K Sbjct: 60 ETQGLTERYLGTWLKKS--GKRDQLLIATKC-----TGPARKPHNPRHVRGGINNLDRKG 112 Query: 118 LIASCEQSLKRMGLDYVDIF----------------YSHRVDADT-PLEETMGALAHLHR 160 L + SL+R+ LD+VD++ Y H D T P+EET+ L+ + Sbjct: 113 LTEALHGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVK 172 Query: 161 QGKALYVGISSYSPELTRKAAAILKSEGVPLLIHQPN-YSMLNRAIEGGLLPALEELGVG 219 GK +G+S+ + + + G+ ++ N Y++LNR+ E L VG Sbjct: 173 AGKIRTIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVG 232 Query: 220 CIGFSPLAQGLLTSKYLGGV-PEGARVTRAPSLPQRMLSEDNLARIRALNE---IASKRG 275 + +SPLA G+L+ KYL G P G R+T L +R N RA E +A + G Sbjct: 233 LLAYSPLAFGMLSGKYLDGARPAGGRLT----LFERFSRYTNAQAERATAEYVALARQHG 288 Query: 276 QTLAQMAIAWVLRDARVTSALIGARTVEQLDDSLDAVNKLDFTQEELASID 326 AQMA+A+V +TS +IGA T+ QL ++D+ +L ++ LA+I+ Sbjct: 289 LDPAQMALAYVNSRPFLTSNIIGATTLAQLRSNIDSA-ELQLGEDVLAAIE 338 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 71.2 bits (173), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 74/275 (26%), Positives = 116/275 (42%), Gaps = 50/275 (18%) Query: 21 RSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPPAGSAEENFGR 80 R+G +P + G W L+ D G++ D A Y AE R Sbjct: 10 RNGQSVPILGQGTWNMGESPAQANAEVRALQAGLDLGMSLIDTAEMY------AEGGAER 63 Query: 81 WMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGARKHLIASCEQSLKRMGLDYVDIFYSH 140 +AK RD++ + SK P+ + +++ IA+CE SLKR+G D +D++ H Sbjct: 64 IVAKAIAGRRDQVYLVSKVL------PH---NASKRGTIAACEASLKRLGTDRIDLYLLH 114 Query: 141 RVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAAILKS-EGVPLLIHQPNYS 199 A PL T+ + L QGK G+S++ T +L+ G L +Q Y+ Sbjct: 115 WRGAH-PLAATIEGMETLVSQGKIGGWGVSNFD---TDDIDDLLEEPHGEHYLCNQVLYN 170 Query: 200 MLNRAIEGGLLPALEELGVGCIGFSPLAQGLLTSKYLGGVPEGARVTRAPSLPQRMLSED 259 + R IE LLP ++ + + +SP+ QG R+ + P Sbjct: 171 LSRRGIEYDLLPQAQQAHIAVMAYSPIEQG--------------RILKHP---------- 206 Query: 260 NLARIRALNEIASKRGQTLAQMAIAWVLRDARVTS 294 AL +A T AQ+A+AWVLR V + Sbjct: 207 ------ALTALAQAHRVTPAQIALAWVLRQPGVVA 235 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,128,786 Number of extensions: 48768 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 138 Number of HSP's successfully gapped: 6 Length of query: 344 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 258 Effective length of database: 1,212,660 Effective search space: 312866280 Effective search space used: 312866280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)