BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0241.p (444 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport tran... 859 0.0 gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport tran... 390 e-109 gi|300313118|ref|YP_003777210.1| proton glutamate symport protei... 219 4e-58 >gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 445 Score = 859 bits (2219), Expect = 0.0, Method: Compositional matrix adjust. Identities = 442/444 (99%), Positives = 443/444 (99%) Query: 1 VSKLFKSLFGQVVLALIGGIIIGLLWPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGI 60 VSKLFKSLFGQVVLALIGGIIIGL WPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGI Sbjct: 2 VSKLFKSLFGQVVLALIGGIIIGLFWPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGI 61 Query: 61 CGASDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVQPGAGMNIDPSNLDASSLSGYM 120 CGASDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVQPGAGMNIDPSNLDASSLSGYM Sbjct: 62 CGASDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVQPGAGMNIDPSNLDASSLSGYM 121 Query: 121 ANAGKVKETGFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALV 180 ANAGKVKETGFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALV Sbjct: 122 ANAGKVKETGFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALV 181 Query: 181 ASLSDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVL 240 ASLSDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVL Sbjct: 182 ASLSDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVL 241 Query: 241 GGILRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMKKLEHMGIKKSTVGLVIPTGY 300 GGILRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMKKLEHMGIKKSTVGLVIPTGY Sbjct: 242 GGILRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMKKLEHMGIKKSTVGLVIPTGY 301 Query: 301 SFNLDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAATL 360 SFNLDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAATL Sbjct: 302 SFNLDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAATL 361 Query: 361 TTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWEKDIDKVRARAVLNGES 420 TTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWE+DIDKVRARAVLNGES Sbjct: 362 TTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWERDIDKVRARAVLNGES 421 Query: 421 VAPLVEENPVIELMGVTVKPAQLG 444 VAPLVEENPVIELMGVTVKPAQLG Sbjct: 422 VAPLVEENPVIELMGVTVKPAQLG 445 >gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 390 bits (1001), Expect = e-109, Method: Compositional matrix adjust. Identities = 211/418 (50%), Positives = 298/418 (71%), Gaps = 3/418 (0%) Query: 4 LFKSLFGQVVLALIGGIIIGLLWPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGICGA 63 L+KSL+ QV++A++ GI++G +P G+ +KPLGDGF+KLIKMII ++FC VV GI G Sbjct: 8 LYKSLYFQVLVAIVIGILLGHFYPSTGEAMKPLGDGFVKLIKMIIAPVIFCTVVIGIAGM 67 Query: 64 SDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVQPGAGMNIDPSNLDASSLSGYMANA 123 D+KKVG G A++YFEVV+T+AL++GL+L +QPG GMN+DP++LD S++ Y A Sbjct: 68 EDMKKVGKTGGLALLYFEVVSTVALIIGLLLVNFLQPGVGMNVDPASLDTKSIAAYTA-P 126 Query: 124 GKVKETGFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALVASL 183 GK+ +F++ +IP + V AF GDVLQVLL++V FG AL G +G V + + Sbjct: 127 GKMGSV--TDFVLGIIPTSMVDAFAKGDVLQVLLVAVLFGFALHKFGGRGTLVFDFIEKI 184 Query: 184 SDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVLGGI 243 S F + +++AP+G GA++FT+GKYG+GSL LA L+ FY + F+ VVLG I Sbjct: 185 SHVLFSVVGAIMKVAPIGAFGAMSFTIGKYGVGSLFSLAKLMGTFYLTCLLFIFVVLGII 244 Query: 244 LRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMKKLEHMGIKKSTVGLVIPTGYSFN 303 R G SI+K ++Y++EEL++VL T+SS+SVLP+++ K+E+ G KK+ VGLVIPTGYSFN Sbjct: 245 TRLHGFSIWKFVKYIKEELLIVLGTSSSESVLPRMLAKMENAGAKKTVVGLVIPTGYSFN 304 Query: 304 LDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAATLTTI 363 LD +IYLTMAA+FIAQATNT ++ +L + L+TSKGA G+ GS ++LAATL+ + Sbjct: 305 LDGTAIYLTMAAVFIAQATNTPMTFVQELTLLGVLLLTSKGAAGITGSGFIVLAATLSAV 364 Query: 364 PAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWEKDIDKVRARAVLNGESV 421 +PV GL L+L ID F+ ARAL N +GN VAT+V+A W ++D R VLN E+V Sbjct: 365 GHVPVAGLALILGIDRFMSEARALTNTIGNGVATLVVAKWSGELDSERLTKVLNNETV 422 >gi|300313118|ref|YP_003777210.1| proton glutamate symport protein [Herbaspirillum seropedicae SmR1] Length = 431 Score = 219 bits (557), Expect = 4e-58, Method: Compositional matrix adjust. Identities = 145/406 (35%), Positives = 229/406 (56%), Gaps = 32/406 (7%) Query: 15 ALIGGIIIGLLWPDFGQNLKPLG-----------DGFIKLIKMIIPVIVFCVVVQGICGA 63 A++ GI++G L F N K D F++LIKM+I +VF +V GI Sbjct: 14 AMLLGILVGYLI--FSHNDKAQAKELAGYISIASDLFLRLIKMVIAPLVFSTLVVGIAHM 71 Query: 64 SDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVQPGAGMNIDPSNLDASSLSGYMANA 123 D K VG + K++ +F + + ++L LG+++A ++QPGAG+ + PS DA+ +G + Sbjct: 72 GDAKSVGRIFGKSLAWFFIASLVSLALGMIMANLLQPGAGVAL-PSP-DAAG-AGLATSK 128 Query: 124 GKVKETGFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALVASL 183 VKE F L+P + V A +VLQV++ S+ FG AL +GE+G ++A++ L Sbjct: 129 FTVKE-----FFNHLVPKSIVEAMAQNEVLQVVVFSMFFGIALASLGERGKHLLAVIDDL 183 Query: 184 SDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVLGGI 243 S K + ++ AP+ V A+A TV G+ L A+ + FY F LV+L I Sbjct: 184 SHTMLKITVYVMKFAPVAVFAAMAATVAVNGLEILVSFAVFMRDFY-----FSLVLLWVI 238 Query: 244 LRASGL-----SIFKLIRYLREELVVVLATTSSDSVLPQIMKKLEHMGIKKSTVGLVIPT 298 L A G IF L+ ++E ++ AT SS++ P+++ L+ G+K+ V+P Sbjct: 239 LIAVGFIFLKKRIFHLLALIKEAFLLAFATASSEAAYPKLLDALDRFGVKRKISSFVMPM 298 Query: 299 GYSFNLDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAA 358 GYSFNLD IY T A LFIAQA H+ + ++ + ++TSKG GVP +++V++AA Sbjct: 299 GYSFNLDGSMIYCTFATLFIAQAYGIHMPISTQITMMLVLMLTSKGIAGVPRASLVVIAA 358 Query: 359 TLTTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWE 404 TL IP GL+++L ID F+ + R+ N +GN +A+ V+A WE Sbjct: 359 TLHQFN-IPEAGLLVILGIDTFLDMGRSATNAVGNSIASAVVAKWE 403 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.142 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,212,056 Number of extensions: 48284 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 248 Number of HSP's successfully gapped: 3 Length of query: 444 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 356 Effective length of database: 1,203,190 Effective search space: 428335640 Effective search space used: 428335640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)