BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0240.p (417 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidoh... 835 0.0 gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase... 416 e-117 gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum ... 163 3e-41 >gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 835 bits (2157), Expect = 0.0, Method: Compositional matrix adjust. Identities = 414/417 (99%), Positives = 416/417 (99%) Query: 1 MKAVPEPFQQAGRQAMQWAQQLAQHTESPGMLTRTYLTPQHQAAAAALARWMEEAGMRVR 60 MKAVPEPFQQAGRQAMQWAQQLAQHTESPGMLTRTYLTPQHQAAAAALARWMEEAGMRVR Sbjct: 1 MKAVPEPFQQAGRQAMQWAQQLAQHTESPGMLTRTYLTPQHQAAAAALARWMEEAGMRVR 60 Query: 61 RDNAGNVIGRYEAAPGHESAPAFVTGSHFDTVRNGGWYDGNLGIVLPLACIARWHRQGQR 120 RDNAGNVIGRYEAAPGHESAP+FVTGSHFDTVRNGGWYDGNLGIVLPLACIARWHRQGQR Sbjct: 61 RDNAGNVIGRYEAAPGHESAPSFVTGSHFDTVRNGGWYDGNLGIVLPLACIARWHRQGQR 120 Query: 121 FAFPIEVIGFAEEEGVRFKATLLGSRTVAGTFDQAVLENRDSDGVTMRAAIRAAGLDPDG 180 FAFPIEVIGFAEEEGVRFKATLLGSRTVAGTFDQAVLENRDSDGVTMRAAIRAAGLDPDG Sbjct: 121 FAFPIEVIGFAEEEGVRFKATLLGSRTVAGTFDQAVLENRDSDGVTMRAAIRAAGLDPDG 180 Query: 181 IAADAWQPGSMAAFVEVHIEQGPLLLNEDLPVGVVTAISGASRFMAEVHGLAGHAGTVPM 240 IAADAWQPGSMAAFVEVHIEQGPLLLNEDLPVGVVTAISGASRFMAEVHGLAGHAGTVPM Sbjct: 181 IAADAWQPGSMAAFVEVHIEQGPLLLNEDLPVGVVTAISGASRFMAEVHGLAGHAGTVPM 240 Query: 241 HLRRDAAMAAAEIGLYIERRCSIKPGLVGTMGLLEVVQGAANVVPGLASFSIDIRAEDDS 300 HLRRDAAMAAAEIGLYIERRCSIKP LVGTMGLLEVVQGAANVVPGLASFSIDIRAEDDS Sbjct: 241 HLRRDAAMAAAEIGLYIERRCSIKPELVGTMGLLEVVQGAANVVPGLASFSIDIRAEDDS 300 Query: 301 DRLAAVADVKAEIDRIAARRQVQVALRQTHEAASVPCAPRLQAALAQSIAAAGWPVRHLP 360 DRLAAVADVKAEIDRIAARRQVQ+ALRQTHEAASVPCAPRLQAALAQSIAAAGWPVRHLP Sbjct: 301 DRLAAVADVKAEIDRIAARRQVQLALRQTHEAASVPCAPRLQAALAQSIAAAGWPVRHLP 360 Query: 361 SGAGHDAMALAGLADVAMLFVRCGNGGISHHPDETMTEADAAIAAQVFSHLVEQFQP 417 SGAGHDAMALAGLADVAMLFVRCGNGGISHHPDETMTEADAAIAAQVFSHLVEQFQP Sbjct: 361 SGAGHDAMALAGLADVAMLFVRCGNGGISHHPDETMTEADAAIAAQVFSHLVEQFQP 417 >gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase/N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 587 Score = 416 bits (1069), Expect = e-117, Method: Compositional matrix adjust. Identities = 213/407 (52%), Positives = 275/407 (67%), Gaps = 4/407 (0%) Query: 12 GRQAMQWAQQLAQHTESPGMLTRTYLTPQHQAAAAALARWMEEAGMRVRRDNAGNVIGRY 71 G MQWA+++ +E G LT TYLT HQ AA +A+WM EAGM+V D GNV+GRY Sbjct: 180 GATVMQWAEEIGALSEEEGALTCTYLTETHQRTAAQIAQWMREAGMQVHIDAVGNVVGRY 239 Query: 72 EAAPGHESAPAFVTGSHFDTVRNGGWYDGNLGIVLPLACIARWHRQGQRFAFPIEVIGFA 131 ++ H A +TGSH+DTVRNGG YDG GI+LP+A + H +G+ F +EVIGF+ Sbjct: 240 QSP--HPQAKTLLTGSHYDTVRNGGKYDGREGILLPIAVVKYLHERGETLPFHLEVIGFS 297 Query: 132 EEEGVRFKATLLGSRTVAGTFDQAVLENRDSDGVTMRAAIRAAGLDPDGIAADAWQPGSM 191 EEEGVRFK+T LGS + G FD A+L+ RD + VTMR I AG D I A P + Sbjct: 298 EEEGVRFKSTFLGSNAITGHFDMALLDLRDKEDVTMRELIIQAGHDVAAIPQIARDPAEI 357 Query: 192 AAFVEVHIEQGPLLLNEDLPVGVVTAISGASRFMAEVHGLAGHAGTVPMHLRRDAAMAAA 251 A+VEVHIEQGP+LL DLPVG+VT+I+G+SR++ ++ G+A HAGT PM +R+DAA AAA Sbjct: 358 LAYVEVHIEQGPVLLKRDLPVGIVTSIAGSSRYLVDLKGVASHAGTTPMDMRKDAAAAAA 417 Query: 252 EIGLYIERRCSIKP--GLVGTMGLLEVVQGAANVVPGLASFSIDIRAEDDSDRLAAVADV 309 EI LY+E+RCS LVGT+G L+V +G+ NV+PG S+DIRA D RLAAV D+ Sbjct: 418 EIILYVEQRCSQDQHAALVGTVGQLQVPRGSTNVIPGACQLSLDIRAATDEVRLAAVKDI 477 Query: 310 KAEIDRIAARRQVQVALRQTHEAASVPCAPRLQAALAQSIAAAGWPVRHLPSGAGHDAMA 369 A+I+ I RR + + L +T A + PCAP L A L+ + AG P L SGAGHDAMA Sbjct: 478 LAKIEEICTRRSIDLKLEETLSAPAAPCAPWLMAQLSAATQRAGVPPFELASGAGHDAMA 537 Query: 370 LAGLADVAMLFVRCGNGGISHHPDETMTEADAAIAAQVFSHLVEQFQ 416 + L DV MLF RCGNGGISH+P ETMT DA ++ Q+ + F+ Sbjct: 538 MVRLTDVCMLFTRCGNGGISHNPLETMTADDAEVSGQILLDFLRNFK 584 >gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 163 bits (412), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 117/373 (31%), Positives = 177/373 (47%), Gaps = 21/373 (5%) Query: 30 GMLTRTYLTPQHQAAAAALARWMEEAGMRVRRDNAGNVIGRYEAAPGHESA-PAFVTGSH 88 G + R LT + ++ W GM V D GN+ R PG +A P +TGSH Sbjct: 33 GGVCRLALTDLDKQGRDLVSSWARAEGMSVVVDQIGNIFMR---RPGRNNALPPVMTGSH 89 Query: 89 FDTVRNGGWYDGNLGIVLPLACIARWHRQGQRFAFPIEVIGFAEEEGVRFKATLLGSRTV 148 DT GG +DGN G++ + + ++ + PIEV + EEG RF ++GS Sbjct: 90 IDTQPTGGKFDGNYGVLAGIEVVRTLNQHNIQTEAPIEVAVWTNEEGSRFVPVMMGSGVF 149 Query: 149 AGTF--DQAVLENRDSDGVTMRAAIRAAGLDPDGIAADAWQPGSMAAFVEVHIEQGPLLL 206 G F D A D +G +R + G + + D + A+ E HIEQGP+L Sbjct: 150 CGAFTLDHA-YNATDVEGKKVRDELERIGYIGEQVCGDH----PIGAYYEAHIEQGPVLE 204 Query: 207 NEDLPVGVVTAISGASRFMAEVHGLAGHAGTVPMHLRRDAAMAAAEIGLYIERRC----- 261 N D +GVV A+ G S + V G HAG PM +R+DA ++ YI + Sbjct: 205 NADTVIGVVPAVLGLSWYDCTVTGFESHAGPTPMEIRKDAL----QVSTYIMQEVVAIAH 260 Query: 262 SIKPGLVGTMGLLEVVQGAANVVPGLASFSIDIRAEDDSDRLAAVADVKAEIDRIAARRQ 321 P GT+G ++V+ + NV+PG FSID+R ++ D+ A ++ + + Sbjct: 261 RYAPYGRGTVGQVQVMPNSRNVIPGQVKFSIDLRNVSEASLTRMHEDLLAYLEAMRKKTG 320 Query: 322 VQVALRQTHEAASVPCAPRLQAALAQSIAAAGWPVRHLPSGAGHDAMALAGLADVAMLFV 381 + + L + P P + + AA G+ + SGAGHDA+ A LA M+FV Sbjct: 321 LSIELERVSYFPPCPFHPDCVDKVRAASAALGYKTMDVVSGAGHDAIYAARLAPAGMIFV 380 Query: 382 RCGNGGISHHPDE 394 C + G+SH+ E Sbjct: 381 PCKD-GVSHNEIE 392 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,376,630 Number of extensions: 60714 Number of successful extensions: 234 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 3 Length of query: 417 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 329 Effective length of database: 1,203,190 Effective search space: 395849510 Effective search space used: 395849510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)