BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0239.p (542 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313821|ref|YP_003777913.1| histidine ammonia-lyase [Herbas... 1110 0.0 gi|300313003|ref|YP_003777095.1| histidine ammonia-lyase [Herbas... 194 2e-50 >gi|300313821|ref|YP_003777913.1| histidine ammonia-lyase [Herbaspirillum seropedicae SmR1] Length = 542 Score = 1110 bits (2872), Expect = 0.0, Method: Compositional matrix adjust. Identities = 538/542 (99%), Positives = 540/542 (99%) Query: 1 MSTITIDGFNLTAAQVVSVARAPHLKVALADSSRAALKQSRDYIESTWMHDEAPMMYSFN 60 MSTITIDGFNLTAAQVVSVARAPHLKVALADSSRAALKQSRDYIESTWMHDEAPMMYSFN Sbjct: 1 MSTITIDGFNLTAAQVVSVARAPHLKVALADSSRAALKQSRDYIESTWMHDEAPMMYSFN 60 Query: 61 TGVGLLKDTRIKVEHIELFQTQLIRAHSAGIGEAFSEEVSRATMLLRANAFASNYSAPRV 120 TGVGLLKDTRIKVEHIELFQTQLIRAHSAGIGEAFSEEVSRATMLLRANAFASNYSAPRV Sbjct: 61 TGVGLLKDTRIKVEHIELFQTQLIRAHSAGIGEAFSEEVSRATMLLRANAFASNYSAPRV 120 Query: 121 EVVDRLLAFINAGIHPIMPQKGSVGASGDLAPLAYLAAAIAGFDEAEVMYQGQRMRATEA 180 EVVDRLLAFINAGIHPIMPQKGSVGASGDLAPLAYLAAAIAGFDEAEVMYQGQRMRATEA Sbjct: 121 EVVDRLLAFINAGIHPIMPQKGSVGASGDLAPLAYLAAAIAGFDEAEVMYQGQRMRATEA 180 Query: 181 IEQAGIGPVKFDLKAKDASALINGCTASLAVAILAAHDARHLLTDACLSLGLTLEAMRAE 240 IEQAGIGPVKFDLKAKDASALINGCTASLAVAILAAHDARHLLTDACLSLGLTLEAMRAE Sbjct: 181 IEQAGIGPVKFDLKAKDASALINGCTASLAVAILAAHDARHLLTDACLSLGLTLEAMRAE 240 Query: 241 MSAFDPRIQQARPHAGQIKTAEVIRTLLQGSTRTTPGARAVQLPEELRRTDIAYTARIQD 300 MSAFDPRIQQARPHAGQIKTAEVIRTLLQGSTRTT ARAVQLP+ELRRTDIAYTARIQD Sbjct: 241 MSAFDPRIQQARPHAGQIKTAEVIRTLLQGSTRTTHAARAVQLPDELRRTDIAYTARIQD 300 Query: 301 VYSLRCAPQVYGPVFDALDYIDTIIEKETNSATDNPLIFHKEEGEGFEIISGGNFHGQYL 360 VYSLRCAPQVYGPVFDALDYIDTIIEKETNSATDNPLIFHKEEGEGFEIISGGNFHGQYL Sbjct: 301 VYSLRCAPQVYGPVFDALDYIDTIIEKETNSATDNPLIFHKEEGEGFEIISGGNFHGQYL 360 Query: 361 AQAMDLLAMAITDLGSICERRIARLIDPTLSWGLPRNLMSGVRGVNTGYPVVQCSMSSLV 420 AQAMDLLAMAITDLGSICERRIARLIDPTLSWGLPRNLMSGVRGVNTGYPVVQCSMSSLV Sbjct: 361 AQAMDLLAMAITDLGSICERRIARLIDPTLSWGLPRNLMSGVRGVNTGYPVVQCSMSSLV 420 Query: 421 MENRTLCMPGSVDSIPSKGNSEDHISNSTWCARKAMTVVQNTQYIVGVEMLLAAQALTMT 480 MENRTLCMPGSVDSIPSKGNSEDHISNSTWCARKAMTVVQNTQYIVGVEMLLAAQALTMT Sbjct: 421 MENRTLCMPGSVDSIPSKGNSEDHISNSTWCARKAMTVVQNTQYIVGVEMLLAAQALTMT 480 Query: 481 EALLKDFVLGQGTQAAYAEIRRQIPACLDGDRWFHDDVEAAHGFVVSGSVRNAVLAAIGE 540 EALLKDFVLGQGTQAAYAEIRRQIPACLDGDRWFHDDV+AAHGFVVSGSVRNAVLAAIGE Sbjct: 481 EALLKDFVLGQGTQAAYAEIRRQIPACLDGDRWFHDDVQAAHGFVVSGSVRNAVLAAIGE 540 Query: 541 FV 542 FV Sbjct: 541 FV 542 >gi|300313003|ref|YP_003777095.1| histidine ammonia-lyase [Herbaspirillum seropedicae SmR1] Length = 526 Score = 194 bits (492), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 138/450 (30%), Positives = 229/450 (50%), Gaps = 33/450 (7%) Query: 34 RAALKQSRDYIESTWMHDEAPMMYSFNTGVGLLKDTRIKVEHIELFQTQLIRAHSAGIGE 93 RA + + D+++ D +Y TG G + E + L H G+G Sbjct: 46 RARIARGADFLDRLLREDGT--IYGVTTGYGDSCTVTVPPELVPELPHHLYTYHGCGLGA 103 Query: 94 AFSEEVSRATMLLRANAFASNYSAPRVEVVDRLLAFINAGIHPIMPQKGSVGASGDLAPL 153 F+ E +RA + R N+ +S V +++++ + + P +P +GSVGASGDL PL Sbjct: 104 LFTPEQARAILAARLNSLCQGFSGVSVALLEQITGLLQHDLLPQIPCEGSVGASGDLTPL 163 Query: 154 AYLAAAIAGFDEAEVMYQGQRMRATEAIEQAGIGPVKFDLKAKDASALINGCTASLAVAI 213 +YLAA + G E +V +G + A +A+ AG+ P++ L+ K+ A++NG A+A Sbjct: 164 SYLAAVLCG--ERDVWREGATVPAAQALAAAGMTPLR--LRPKEGLAIMNGTAVMTALAC 219 Query: 214 LAAHDARHLLTDACLSLGLTLEAMRAEMSAFDPRIQQARPHAGQIKTAEVIRTLLQGSTR 273 LA AR+L A ++ + FD + A+PH GQ + A ++ R Sbjct: 220 LAFDRARYLCQLATRITAMSSFTLDGNAHHFDATLFSAKPHPGQQQVAAWLQ-------R 272 Query: 274 TTPGARAVQLPEELRRTDIAYTARIQDVYSLRCAPQVYGPVFDALDYIDTIIEKETNSAT 333 P +A R + R+QD YS+RCAP V G + DAL + IE E NSA Sbjct: 273 DLPCGQA-------HRNE----KRLQDRYSVRCAPHVIGVLNDALPSLRQFIENELNSAN 321 Query: 334 DNPLIFHKEEGEGFEIISGGNFHGQYLAQAMDLLAMAITDLGSICERRIARLIDPTLSWG 393 DNPLI + +G ++ GG+F+G ++A AMD + A+ ++ + +R++A ++D + G Sbjct: 322 DNPLI----DPDGERVLHGGHFYGGHIAFAMDSMKTAVANVADLLDRQMALMVDQRYNNG 377 Query: 394 LPRNLMSGVRG----VNTGYPVVQCSMSSLVMENRTLCMPGSVDSIPSKGNSEDHISNST 449 LP NL SG +G +N G +Q S S+ E L MP SV S ++ +++D +S T Sbjct: 378 LPANL-SGAQGARAPINHGLKALQISASAWTAEALKLTMPASVFSRSTECHNQDKVSMGT 436 Query: 450 WCARKAMTVVQNTQYIVGVEMLLAAQALTM 479 AR + V++ T+ + ++ Q + + Sbjct: 437 IAARDCLRVLELTEQVAAALLITVRQGVWL 466 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,519,770 Number of extensions: 57730 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 2 Length of query: 542 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 452 Effective length of database: 1,193,720 Effective search space: 539561440 Effective search space used: 539561440 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)