BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0230.p (362 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313814|ref|YP_003777906.1| spermidine/putrescine ABC trans... 735 0.0 gi|300310414|ref|YP_003774506.1| spermidine/putrescine ABC trans... 255 3e-69 gi|300309936|ref|YP_003774028.1| spermidine/putrescine ABC trans... 231 5e-62 gi|300312386|ref|YP_003776478.1| sugar ABC transporter ATPase [H... 223 1e-59 gi|300311547|ref|YP_003775639.1| ABC transporter ATPase [Herbasp... 221 8e-59 >gi|300313814|ref|YP_003777906.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 362 Score = 735 bits (1897), Expect = 0.0, Method: Compositional matrix adjust. Identities = 362/362 (100%), Positives = 362/362 (100%) Query: 1 MTELSVNDLHLDYGTGAHANPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPK 60 MTELSVNDLHLDYGTGAHANPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPK Sbjct: 1 MTELSVNDLHLDYGTGAHANPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPK 60 Query: 61 AGAIDIGKRRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSGEI 120 AGAIDIGKRRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSGEI Sbjct: 61 AGAIDIGKRRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSGEI 120 Query: 121 ATRVKAVLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLRE 180 ATRVKAVLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLRE Sbjct: 121 ATRVKAVLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLRE 180 Query: 181 EARAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFT 240 EARAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFT Sbjct: 181 EARAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFT 240 Query: 241 AEFMGSNNKLAAEVLSREGERVTVKLDGGTAVATLRGRSDVRPGEQLTGIIRVEQVRISG 300 AEFMGSNNKLAAEVLSREGERVTVKLDGGTAVATLRGRSDVRPGEQLTGIIRVEQVRISG Sbjct: 241 AEFMGSNNKLAAEVLSREGERVTVKLDGGTAVATLRGRSDVRPGEQLTGIIRVEQVRISG 300 Query: 301 EASDNAIRLPLSTCMYLGDRWECLFKVDGGPAGSVGLRAYAPQRLDQGEYWLSLPEQSLW 360 EASDNAIRLPLSTCMYLGDRWECLFKVDGGPAGSVGLRAYAPQRLDQGEYWLSLPEQSLW Sbjct: 301 EASDNAIRLPLSTCMYLGDRWECLFKVDGGPAGSVGLRAYAPQRLDQGEYWLSLPEQSLW 360 Query: 361 AF 362 AF Sbjct: 361 AF 362 >gi|300310414|ref|YP_003774506.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 255 bits (652), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 133/308 (43%), Positives = 191/308 (62%), Gaps = 11/308 (3%) Query: 20 NPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPKAGAIDIGKRRVFDGARNFE 79 N I+K + ++++RGE + LLGPSGSGKTT L +AG E P G I + DG Sbjct: 35 NLIVKNLDLDIRRGEFLTLLGPSGSGKTTCLMMLAGFEFPTGGEIRL------DGQLLNR 88 Query: 80 MPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSGEIATRVKAVLSQLGLGHLGER 139 +P +RN+G+VFQ+YAL+PH T+A NVAY L +RK +VK L + +G G+R Sbjct: 89 VPPHKRNIGMVFQNYALFPHMTIAQNVAYPLSVRKMDRATREAKVKQALDMVQMGKFGDR 148 Query: 140 YPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLREEARAFLRELIVRLGLSALM 199 YP QLSGGQQQRVA+AR LV++PQ++L+DEPL LD +LRE + L+ L RLG++ + Sbjct: 149 YPTQLSGGQQQRVALARTLVFDPQLVLMDEPLGALDKQLREHMQLELKALHKRLGVTFVY 208 Query: 200 VTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFTAEFMGSNNKLAAEVLSREG 259 VTHDQ EA+ +SDR+ + + G I+Q +Y+ P+ F A F+G NN+ V S +G Sbjct: 209 VTHDQSEALTMSDRVAVFDQGVIQQLAPVTELYEYPDNQFVANFIGDNNRFKGSVESVQG 268 Query: 260 ERVTVKLDGGTAVATLRGRSDVRPGEQLTGIIRVEQVRIS--GEASDNAIRLPLSTCMYL 317 E TV+L G A+ L G+ +T +R E++R++ G A N+IR ++ +Y Sbjct: 269 EHCTVRLTDGAALTGLNVHR-AHAGQSITACVRPERIRLAAGGSAEPNSIRASIAGLIYF 327 Query: 318 GD--RWEC 323 GD R C Sbjct: 328 GDHVRVRC 335 >gi|300309936|ref|YP_003774028.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 361 Score = 231 bits (590), Expect = 5e-62, Method: Compositional matrix adjust. Identities = 135/307 (43%), Positives = 189/307 (61%), Gaps = 18/307 (5%) Query: 17 AHANPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPKAGAIDIGKRRVFDGAR 76 A+ L+ + +++ GE + LLGPSG GKTT LR +AGLE P G RV G Sbjct: 20 ANGTRALQPLDLQIHPGETLVLLGPSGCGKTTTLRMIAGLEFPDEGG------RVLFGEE 73 Query: 77 NFEM-PAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSGEIATRVKAVLSQLGLGH 135 + M P E+R +G+VFQ+YAL+P+ +V +N+AYG+K+RK +GE A RV+ +L + L Sbjct: 74 DVTMLPIEKRGVGMVFQNYALFPNMSVGENIAYGMKIRKIAAGERAERVERLLEMVHLQG 133 Query: 136 LGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLREEARAFLRELIVRLGL 195 L R QLSGGQ+QRVA+ARAL P+V+LLDEPL+ LDAKLRE R+ L +L+ LG+ Sbjct: 134 LSHRRVDQLSGGQKQRVALARALAMEPRVLLLDEPLTALDAKLREAVRSDLNKLLRSLGI 193 Query: 196 SALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFTAEFMGSNNKLAAEVL 255 +A+ VTHDQGEAMA+ DRI+++ G+I Q GTPQ +Y P F AEF+G+ N++ V Sbjct: 194 TAIYVTHDQGEAMALGDRIVVMERGKISQIGTPQEIYYHPANDFVAEFIGAMNRVDGIV- 252 Query: 256 SREGERVTVKLDGGTAVATLRGRSDVRPGEQLTGIIRVEQVRISG--EASDNAIRLPLST 313 G+R+ + TL D G+ + R E V + EA + +R L Sbjct: 253 --AGDRLQLP------TGTLPLPPDAVAGQPAQALFRPEDVEVIDITEADAHCLRGKLIN 304 Query: 314 CMYLGDR 320 +LGDR Sbjct: 305 TFFLGDR 311 >gi|300312386|ref|YP_003776478.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 223 bits (569), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 122/296 (41%), Positives = 183/296 (61%), Gaps = 16/296 (5%) Query: 23 LKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPKAGAIDIGKRRVFDGARNFEMPA 82 ++ + E++ G+ LLGPSG GK+T LR +AGL+ P +G+I IG R V ++P Sbjct: 19 VRNIDFEVKAGQFTVLLGPSGCGKSTTLRLIAGLDKPSSGSIHIGGRDVT------QLPP 72 Query: 83 EERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSGEIATRVKAVLSQLGLGHLGERYPH 142 +R + +VFQSYAL+PH +V DN+ +G+++RK P TR+K V LGL HL ER P Sbjct: 73 AQRKISMVFQSYALFPHLSVRDNILFGVRVRKEPRAGYETRLKRVADILGLAHLLERRPA 132 Query: 143 QLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLREEARAFLRELIVRLGLSALMVTH 202 QLSGGQQQRVA+ RA++ + V L+DEPLSNLDA+LR+E R +R L +L ++ + VTH Sbjct: 133 QLSGGQQQRVALGRAIIADAPVCLMDEPLSNLDAQLRQEMRREIRALQQQLNMTMVYVTH 192 Query: 203 DQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFTAEFMGSNNKLAAEVLSREGERV 262 DQ EAM+++D+++LL GRIEQ P +Y P T F A F+G+ +L E Sbjct: 193 DQTEAMSMADQVILLREGRIEQDAAPADLYAQPATAFAARFIGTP---PMNLLPLEPHAE 249 Query: 263 TVKLDGGTAVATLRGRSDVRPGEQLTGIIRVEQVRISGEASDNAIRLPLSTCMYLG 318 + + G + G++ + G +R E +R++ +ASD+++ + T Y G Sbjct: 250 GLVISGSDVILRDLGQTPWQLG------LRPEHIRLA-DASDSSVPAVVETVEYFG 298 >gi|300311547|ref|YP_003775639.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 221 bits (563), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 153/367 (41%), Positives = 217/367 (59%), Gaps = 29/367 (7%) Query: 4 LSVNDLHLDYGTGAHANPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPKAGA 63 LSV ++ +G+ L VS+E++RGE+V LLGPSG GKTTLLR +AGLE +G Sbjct: 13 LSVREVEKRFGSFT----ALDRVSLEVRRGEMVCLLGPSGCGKTTLLRTIAGLERQDSGR 68 Query: 64 IDIGKRRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGL-KLRKTPSGEIAT 122 + DG ++P + R+ G++FQSYAL+P+ +VADNVAYGL ++ + E Sbjct: 69 LHA------DGRDISQLPPQARDYGILFQSYALFPNLSVADNVAYGLGRMSRQQKRE--- 119 Query: 123 RVKAVLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLREEA 182 RV +LS +GL ++YP QLSGGQQQRVA+ARAL +P ++LLDEPLS LDA++RE Sbjct: 120 RVTEMLSMVGLDGSQDKYPGQLSGGQQQRVALARALAPSPSLLLLDEPLSALDAQVREHL 179 Query: 183 RAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFTAE 242 + +R L + ++ LMVTHDQ EAM ++DRI ++ +GRIEQ GTP+ +Y+ P T F A+ Sbjct: 180 QLEIRRLQKQFRITTLMVTHDQEEAMVMADRIAVMQHGRIEQFGTPEQIYRRPATPFVAD 239 Query: 243 FMGSNNKLAAEVLSREGERVTVKLDGGTAVATLRGRSDVRPGEQLTGIIRVEQVRI-SGE 301 F+G N L LS G +V V GG + SD G R E V++ S E Sbjct: 240 FIGQANWLPFTRLS--GSQVAV---GGLHLEIQPDESDRASGRLFC---RPEAVQLFSDE 291 Query: 302 ASDNAIRLPLSTCMYLGDRWECLFKVDGGPAGSV--GLRAYAPQRL----DQGEYWLSLP 355 + N + +YLGDR+ D P ++ + + A +R+ Q W++LP Sbjct: 292 SCANRFLARVVDRIYLGDRYRLALAADALPGQTLLADVSSQAHERVASLDTQDGLWVTLP 351 Query: 356 EQSLWAF 362 QSL F Sbjct: 352 RQSLQVF 358 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,206,463 Number of extensions: 53448 Number of successful extensions: 626 Number of sequences better than 1.0e-08: 133 Number of HSP's gapped: 381 Number of HSP's successfully gapped: 152 Length of query: 362 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 276 Effective length of database: 1,212,660 Effective search space: 334694160 Effective search space used: 334694160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)