BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0226.p
         (323 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase...   637   0.0  
gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase...    91   1e-19
gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase...    65   6e-12

>gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 323

 Score =  637 bits (1642), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 318/323 (98%), Positives = 319/323 (98%)

Query: 1   MQQQVSPKGKPKSGIARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIAR 60
           MQQQVS KGK KSGIARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIAR
Sbjct: 1   MQQQVSIKGKTKSGIARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIAR 60

Query: 61  HWPSLQALGLAEHASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQ 120
           HWPSLQALGLAEHASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQ
Sbjct: 61  HWPSLQALGLAEHASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQ 120

Query: 121 VVLASSSSGLLMSKVQAACRHPQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFY 180
           VVLASSSSGLLMSKVQAACRHPQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFY
Sbjct: 121 VVLASSSSGLLMSKVQAACRHPQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFY 180

Query: 181 AAIGKKPIHVRKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMG 240
           AAIGKKPIHVRKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMG
Sbjct: 181 AAIGKKPIHVRKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMG 240

Query: 241 PYLNLHMSGGEGGIQHLLDHLGAPIESWWADLGAPALTDALKATVRAGVEEQMTGKSSTD 300
           PYLNLHMSGGEGGIQHLLDHLGAPIESWWADLGAPALTDALKATVRAGVEEQM GK+STD
Sbjct: 241 PYLNLHMSGGEGGIQHLLDHLGAPIESWWADLGAPALTDALKATVRAGVEEQMAGKTSTD 300

Query: 301 IAAERDQLLVQLLAAKSWARVIP 323
           IAAERDQLLVQLLAAKS ARVIP
Sbjct: 301 IAAERDQLLVQLLAAKSRARVIP 323


>gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase
           [Herbaspirillum seropedicae SmR1]
          Length = 512

 Score = 91.3 bits (225), Expect = 1e-19,   Method: Compositional matrix adjust.
 Identities = 78/247 (31%), Positives = 116/247 (46%), Gaps = 13/247 (5%)

Query: 1   MQQQVSPKGKPKSGIARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIAR 60
           M  +  P    +S +  VAV+G+G +GA  A      G  V   D    A  K   AI  
Sbjct: 1   MNNRKYPHALSRSAL--VAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAAGKAIGAICT 58

Query: 61  HWPSLQAL-----GLAEHASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDA 115
           +   L +       LA+ ASQ R+     LE  L  AD V E   E+   K +LF +++A
Sbjct: 59  NLDKLVSKDKLTPALAQAASQ-RISVAQSLEE-LHQADLVIEAIVEQLAAKHELFGKLEA 116

Query: 116 ALPAQVVLASSSSGLLMSKVQAACRHPQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQT 175
            +  + +LA+++S L ++++ A  +HP+RVV  H FNP  ++ LVE+I G  +     Q 
Sbjct: 117 IVSTRCILATNTSSLSITQIAAGLQHPERVVGMHFFNPVPMMALVEIISGLASDAALAQC 176

Query: 176 ALDFYAAIGKKPIHVRKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLR 235
             D   A GK P+  R    G I NRL    + E   ++ +G A    ID  +    G R
Sbjct: 177 LHDTALAWGKTPVMARS-TPGFIVNRLARPYYAEGLRMLHEGGAQPATIDAVMREAGGFR 235

Query: 236 WALMGPY 242
              MGP+
Sbjct: 236 ---MGPF 239


>gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase
           oxidoreductase [Herbaspirillum seropedicae SmR1]
          Length = 805

 Score = 65.5 bits (158), Expect = 6e-12,   Method: Compositional matrix adjust.
 Identities = 54/195 (27%), Positives = 90/195 (46%), Gaps = 8/195 (4%)

Query: 15  IARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIARHWPSLQAL-----G 69
           + +VAV+G GV+GA  AA+ +   + V   D +P  E      + R   +L+ L     G
Sbjct: 16  VKKVAVLGAGVMGAQIAAHCINARVPVVLFD-LPAKEGPKNGIVQRAIENLKKLSPAPLG 74

Query: 70  LAEHASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQVVLASSSSG 129
             + A+  ++  Y+   A LEG D + E   ER D K DL++++   +  + + AS++SG
Sbjct: 75  NKDDAALIQVANYEDDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFASNTSG 134

Query: 130 LLMSKVQAACRH--PQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFYAAIGKKP 187
           L ++ +          R    H FNPP  + LVE+I    T P  +     F      K 
Sbjct: 135 LSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTTLGKG 194

Query: 188 IHVRKEVKGHIANRL 202
           +    +    +ANR+
Sbjct: 195 VVRAFDTPNFVANRV 209


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.318    0.133    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,121,579
Number of extensions: 50997
Number of successful extensions: 246
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 242
Number of HSP's successfully gapped: 3
Length of query: 323
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 238
Effective length of database: 1,217,395
Effective search space: 289740010
Effective search space used: 289740010
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 131 (55.1 bits)