BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0218.p (125 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313805|ref|YP_003777897.1| two component response regulato... 245 1e-66 gi|300313791|ref|YP_003777883.1| response regulator protein [Her... 66 1e-12 gi|300310669|ref|YP_003774761.1| two component response regulato... 57 3e-10 >gi|300313805|ref|YP_003777897.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 125 Score = 245 bits (625), Expect = 1e-66, Method: Compositional matrix adjust. Identities = 124/125 (99%), Positives = 124/125 (99%) Query: 1 MPSSHGAIAVIDDDHGVRAALCNLLDSAGYRSCRFSSGEEFLASDCMPVAVCAIIDLQLA 60 MPSSHGAIAVIDDDHGVRAALCNLLDSAGYRSCRFSSGEEFLASDCMPVAVCAIIDLQLA Sbjct: 1 MPSSHGAIAVIDDDHGVRAALCNLLDSAGYRSCRFSSGEEFLASDCMPVAVCAIIDLQLA 60 Query: 61 CMSGFELAERLALARPDLPLLFISAQVTPHQQQRASALGGALLAKPVDADTLLALLTACL 120 CMSGFELAERLALARPDLPLLFISAQVTP QQQRASALGGALLAKPVDADTLLALLTACL Sbjct: 61 CMSGFELAERLALARPDLPLLFISAQVTPRQQQRASALGGALLAKPVDADTLLALLTACL 120 Query: 121 LYGRP 125 LYGRP Sbjct: 121 LYGRP 125 >gi|300313791|ref|YP_003777883.1| response regulator protein [Herbaspirillum seropedicae SmR1] Length = 206 Score = 65.9 bits (159), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 41/115 (35%), Positives = 60/115 (52%), Gaps = 1/115 (0%) Query: 7 AIAVIDDDHGVRAALCNLLDSAGYRSCRFSSGEEFLASDCMPVAVCAIIDLQLACMSGFE 66 + V+DDD+ VR AL LL SAGY + F+S +EFL + V C ++D+++ GFE Sbjct: 4 TVFVVDDDNMVRTALLRLLASAGYAAHGFASADEFLQARLPAVPCCLLLDMKMPGSDGFE 63 Query: 67 LAERLALARPDLPLLFISAQVT-PHQQQRASALGGALLAKPVDADTLLALLTACL 120 + RL +P++FIS T P Q A L KP+ + LL + L Sbjct: 64 VVRRLERRGDAIPVIFISGYGTIPTTVQAMKAGAHEFLTKPIQDERLLGAVEEAL 118 >gi|300310669|ref|YP_003774761.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 214 Score = 57.4 bits (137), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 36/123 (29%), Positives = 60/123 (48%), Gaps = 1/123 (0%) Query: 1 MPSSHGAIAVIDDDHGVRAALCNLLDSAGYRSCRFSSGEEFLASDCMPVAVCAIIDLQLA 60 M ++ + ++DDD VR +L LL S R+ F E FL + C I+D+++ Sbjct: 2 METNPPVVYIVDDDEAVRTSLAWLLSSVNIRTECFEGPEAFLKAYDANSLSCLILDVRMP 61 Query: 61 CMSGFELAERLALARPDLPLLFISAQVTPHQQQRASALGGA-LLAKPVDADTLLALLTAC 119 +SGF+L +RL D+P++F+S RA G L KP ++ +L + Sbjct: 62 EISGFQLQDRLKEIGADIPVIFVSGHGDIPMSVRALHNGAVDFLEKPYNSQQMLERIQMT 121 Query: 120 LLY 122 L + Sbjct: 122 LKH 124 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.137 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 283,027 Number of extensions: 7735 Number of successful extensions: 73 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 78 Number of HSP's successfully gapped: 3 Length of query: 125 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 50 Effective length of database: 1,264,745 Effective search space: 63237250 Effective search space used: 63237250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 125 (52.8 bits)