BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0217.p (80 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313804|ref|YP_003777896.1| transmembrane protein [Herbaspi... 158 9e-41 gi|300313803|ref|YP_003777895.1| transmembrane protein [Herbaspi... 55 1e-09 >gi|300313804|ref|YP_003777896.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 80 Score = 158 bits (399), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 80/80 (100%), Positives = 80/80 (100%) Query: 1 MKPYLISLFMGILAGLIYAALHVQSPAPPIIALLGLLGMLIGEQVVPIVRRKLAGEPLTA 60 MKPYLISLFMGILAGLIYAALHVQSPAPPIIALLGLLGMLIGEQVVPIVRRKLAGEPLTA Sbjct: 1 MKPYLISLFMGILAGLIYAALHVQSPAPPIIALLGLLGMLIGEQVVPIVRRKLAGEPLTA 60 Query: 61 SWFHRECMPKITGTPPPEEP 80 SWFHRECMPKITGTPPPEEP Sbjct: 61 SWFHRECMPKITGTPPPEEP 80 >gi|300313803|ref|YP_003777895.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 54 Score = 54.7 bits (130), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 28/45 (62%), Positives = 36/45 (80%) Query: 1 MKPYLISLFMGILAGLIYAALHVQSPAPPIIALLGLLGMLIGEQV 45 M YLISL GIL G++YA V+SPAPP IAL+GLLGM++GE++ Sbjct: 1 MSAYLISLCAGILIGVVYALFRVRSPAPPGIALVGLLGMVLGERL 45 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.144 0.451 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 224,435 Number of extensions: 5569 Number of successful extensions: 26 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 26 Number of HSP's successfully gapped: 2 Length of query: 80 Length of database: 1,619,870 Length adjustment: 51 Effective length of query: 29 Effective length of database: 1,378,385 Effective search space: 39973165 Effective search space used: 39973165 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 123 (52.0 bits)