BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0208.p (325 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 575 e-165 gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 124 1e-29 gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 97 2e-21 gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 87 1e-18 gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane ... 77 2e-15 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 575 bits (1482), Expect = e-165, Method: Compositional matrix adjust. Identities = 318/325 (97%), Positives = 320/325 (98%) Query: 1 MAADWIVRLSAGDAAQQQETAAAFARWQGQSRQHAQAARELQGVLAHCEGLLHSAQGDPS 60 MAADWIVRLSAGDAAQQQETAAAFARWQGQSRQHAQAARELQGVLAHCEGLLHSAQGDPS Sbjct: 18 MAADWIVRLSAGDAAQQQETAAAFARWQGQSRQHAQAARELQGVLAHCEGLLHSAQGDPS 77 Query: 61 PLLAGLESAMALSPRPGRGRRAVAALALACCIGLPLWLAWQAYPARYLLADMRTATGHWD 120 PLLAGLE+AMA SPRPGRGRRAVAALALACCIGLPLWL QAYPARYLLADMRTATGHWD Sbjct: 78 PLLAGLEAAMAPSPRPGRGRRAVAALALACCIGLPLWLGLQAYPARYLLADMRTATGHWD 137 Query: 121 ARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEVARDPARPFVIQTADGSIRAL 180 ARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEVARDPARPFVIQTADGSIRAL Sbjct: 138 ARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEVARDPARPFVIQTADGSIRAL 197 Query: 181 GTRFLVRRDQAGTLVSMLESKVSIQPGADTTQVPLVLEAGQRVRLHGGAPGPLEHIDAAA 240 GTRFLVRRD+AGTLVSMLESKVSIQPGADTTQ PLVLEAGQRVRLHGGA GPLEHIDAAA Sbjct: 198 GTRFLVRRDRAGTLVSMLESKVSIQPGADTTQAPLVLEAGQRVRLHGGAAGPLEHIDAAA 257 Query: 241 LERAWRRHQLVSNARPLTEVLDELNRHRPGRILYDQAQLAGLHMTVVLPLDDTQRALQLL 300 LERAWRRHQLVSNARPLTEVLDELNRHRPGRILYDQAQLAGLHMTVVLPLDDTQRALQLL Sbjct: 258 LERAWRRHQLVSNARPLTEVLDELNRHRPGRILYDQAQLAGLHMTVVLPLDDTQRALQLL 317 Query: 301 VDGVPGLRVRQFTPYLTYIDLAPAS 325 VDGVPGLRVRQFTPYLTYIDLAPAS Sbjct: 318 VDGVPGLRVRQFTPYLTYIDLAPAS 342 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 116/336 (34%), Positives = 168/336 (50%), Gaps = 43/336 (12%) Query: 2 AADWIVRLSAG--DAAQQQETAAAFARWQGQSRQHAQA---ARELQGVLAHCEGLLHSAQ 56 AA W++ + G DAA++ + A+W+ +S +H +A A LQ A Sbjct: 19 AATWLMEMHEGPLDAARRSK----LAQWRQRSPEHERAWSRAALLQETFAA--------- 65 Query: 57 GDPSPLLAGLESAMALSPRPGRGRRAVAALALACCIGLPLWLAWQAYPARYLLADMRTAT 116 L G +A+ GR R+A+ LA A +G WLA+ A A + TA Sbjct: 66 -----LPPGGATALKQVGPAGR-RKAIKLLAYALTLGPAGWLAYHALSGPEQGARIATAI 119 Query: 117 GHWDARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEVARD---PARPFVIQTA 173 G L DGSRL L+ + F +R + L +GE++V A D P RPF++ T Sbjct: 120 GERREVQLDDGSRLWLNTDTRITLDFTPAQRLLRLQRGEIMVLTAPDRQQPPRPFLVATR 179 Query: 174 DGSIRALGTRFLVR-RDQAGTLVSMLESKVSIQPGADTTQVPLVLEAGQRVRLH---GGA 229 GS+R LGTRF VR RDQA V++L+ V I P + Q VL +G + R A Sbjct: 180 QGSLRPLGTRFTVRERDQA-VEVAVLQGAVEITPASSAQQT--VLPSGMQTRFTRSGSAA 236 Query: 230 PGPLEHIDAAALERAWRRHQLVSNARPLTEVLDELNRHRPGRILYDQAQLAGLHMTVVLP 289 P P + A AW R L++ PL + + EL R+R G + D A +AGL ++ V P Sbjct: 237 PRPCDDTQA-----AWSRGLLIAERMPLAQFIGELGRYRSGLLQADPA-IAGLEVSGVFP 290 Query: 290 LDDTQRALQLLVDGVPGLRVRQFTPYLTYIDLAPAS 325 L DT R L+LL +P +RV+Q T Y ++ + PA+ Sbjct: 291 LADTDRVLRLLAQTLP-VRVQQLTRY--WVRVLPAT 323 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 96.7 bits (239), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 88/282 (31%), Positives = 132/282 (46%), Gaps = 14/282 (4%) Query: 20 TAAAFARWQGQSRQHAQAARELQGVLAHCEGLLHSAQGDPSPLLAGLESAMALSPRPGRG 79 T A+ W+ H +A R ++ V + L SP+ A L A AL+P G Sbjct: 40 TLEAWEAWRSAHPDHERAWRRIESVRGRLQAL-------ASPVNAALAQA-ALTP-SGNR 90 Query: 80 RRAVAALALACCIGLPLWLAWQAYPARYLLADMRTATGHWDARTLADGSRLLLSGSSAAN 139 R+ + LAL G W ++ P + AD+RT G L DGS+L+L+ SA N Sbjct: 91 RQVIKTLALLVFGGGVAWGLTRSEPWQQWRADLRTDIGQQRRLVLEDGSQLVLNTHSAVN 150 Query: 140 FHFDAQRRTVELVQGELLVEVAR-DPARPFVIQTADGSIRALGTRFLVRRDQAGTLVSML 198 F A+ R + L+ GELLV + RPF+++TA G ALGTR+ VR+ T VS+ Sbjct: 151 LAFSAEERRLRLISGELLVTTGKAGGGRPFLVETAQGLAYALGTRYAVRQRAGRTEVSVW 210 Query: 199 ESKVSIQPGADTTQVPLVLEAGQRVRLHGGAPGPLEHIDAAALERAWRRHQLVSNARPLT 258 E V + P Q VL+AGQ+ G + D + AW +V+ L Sbjct: 211 EGAVRLVP-RQAAQQARVLQAGQQAAFSAQGVGDVTTADETRM--AWTDGVIVARGMRLD 267 Query: 259 EVLDELNRHRPGRILYDQAQLAGLHMTVVLPLDDTQRALQLL 300 L EL R+ R+ A ++G+ ++ P+ D+ + L Sbjct: 268 AFLAELGRYTTDRLSCAPA-VSGVRISGSYPVADSDAVIATL 308 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 87.4 bits (215), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 95/315 (30%), Positives = 139/315 (44%), Gaps = 24/315 (7%) Query: 5 WIVRLSA--GDAAQQQETAAAFARWQGQSRQHAQAARELQGVLAHCEGLL------HSAQ 56 W+V+L A GDAA E A A W+ +H Q EL + L+ Sbjct: 19 WLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMASEEQFQRLVALPVPASQWH 78 Query: 57 GDPSPLLAGLESAMALSPRPGRGRRAVAALALACCIGLPLWLAWQAYPARYLLADMRTAT 116 G L + + + G + A+AL L A Y T T Sbjct: 79 GPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQAGLFEALDGASYA-----TRT 133 Query: 117 GHWDARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEVARDPA----RPFVIQT 172 G L DG+ L L+ SS+ FD RR + L QGE+ ++ D RPF +QT Sbjct: 134 GDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGADAQWPHHRPFWVQT 193 Query: 173 ADGSIRALGTRFLVRRDQAGTLVSMLESKVSIQ-PGADTTQVPLVLEAGQRVRLHGGAPG 231 A ++ALGTRF VR+ GT +++LE +V++ G D PLV + G+ V +H Sbjct: 194 AHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHVAGVD----PLVAQPGEIVDIHPDGRL 249 Query: 232 PLEHIDAAALE-RAWRRHQLVSNARPLTEVLDELNRHRPGRILYDQAQLAGLHMTVVLPL 290 A+A++ AW LV L +V+ +L R+R G + D A +A ++ V L Sbjct: 250 VRRAARASAMDPTAWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPA-VADWQVSGVFQL 308 Query: 291 DDTQRALQLLVDGVP 305 DT AL+ L +P Sbjct: 309 QDTDVALEALAQALP 323 >gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 77.4 bits (189), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 75/224 (33%), Positives = 103/224 (45%), Gaps = 23/224 (10%) Query: 104 PARYLLADMRTATGHWDARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGELLVEVARD 163 P L AD+ T TG TLADGS + L+ SA + R V L++GE+ V++A Sbjct: 104 PVAALTADLHTGTGQRQRTTLADGSIITLNARSAIDHLISPVLRQVRLLEGEIQVQIAAA 163 Query: 164 PARPFVIQTADGSIRAL-GTRFL----VRRDQAGTLVSMLESKVSIQPGADTTQVPLVLE 218 A PF I+T GS+ A GT L +R D + L +S + G T L Sbjct: 164 NALPFSIETVHGSVSATAGTLMLGLQPLRTDLVAQQAAAL---LSTRNGQHAT-----LA 215 Query: 219 AGQRVRLHG---GAPGPLEHIDAAALERAWRRHQLVSNARPLTEVLDELNRHRPGRILYD 275 AGQR GAP A E +W ++ L EV+ L +R G + D Sbjct: 216 AGQRTWFDQDEIGAPRA-----AGGAENSWVEGYYTASDNSLEEVVAALRPYRKGLLRLD 270 Query: 276 QAQLAGLHMTVVLPLDDTQRALQLLVDGVPGLRVRQFTPYLTYI 319 A +A L ++ PLDDT RAL L +P + V + +PY I Sbjct: 271 PA-VAQLRISGAFPLDDTDRALSALASALP-ITVNRVSPYWVSI 312 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 888,612 Number of extensions: 31804 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 5 Length of query: 325 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 240 Effective length of database: 1,217,395 Effective search space: 292174800 Effective search space used: 292174800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)