BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0196.p (298 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313785|ref|YP_003777877.1| autotransporter adhesin protein... 584 e-168 gi|300312345|ref|YP_003776437.1| cell wall surface anchor protei... 219 2e-58 gi|300310420|ref|YP_003774512.1| inner membrane protein [Herbasp... 210 1e-55 gi|300310421|ref|YP_003774513.1| hypothetical protein Hsero_1088... 203 1e-53 >gi|300313785|ref|YP_003777877.1| autotransporter adhesin protein [Herbaspirillum seropedicae SmR1] Length = 3631 Score = 584 bits (1506), Expect = e-168, Method: Compositional matrix adjust. Identities = 296/298 (99%), Positives = 296/298 (99%), Gaps = 1/298 (0%) Query: 1 VNLTVAQSLAPGEHDIVARYTDLAGNTVNGTAQHVSVAGGGDPVTLTDLAVRSSKQAPAA 60 VNLTVAQSLAPGEHDIVARYTDLAGNTVNGTAQHVSVAGGGDPVTLTDLAVRSSKQAPAA Sbjct: 3335 VNLTVAQSLAPGEHDIVARYTDLAGNTVNGTAQHVSVAGGGDPVTLTDLAVRSSKQAPAA 3394 Query: 61 VQALNASENRYAVINDLDTSDISGHSASGPVISGKVGGGRDSDRYVVTVEMGGKVLAFDE 120 VQALNASENRYAVINDLDTSDISGHSASGPVISGKVGGGRDSDRYVVTVEMGGKVLAFDE Sbjct: 3395 VQALNASENRYAVINDLDTSDISGHSASGPVISGKVGGGRDSDRYVVTVEMGGKVLAFDE 3454 Query: 121 VGAGDFSISLSAGSLAPGLYRDLTVTASRASGNEPGQSTAVQGLKLGWYWAAQSVSDIFG 180 VGAGDFSISLSAGSLAPGLYRDLTVTASRASGNEPGQSTAVQGLKLGWYWAAQSVSDIFG Sbjct: 3455 VGAGDFSISLSAGSLAPGLYRDLTVTASRASGNEPGQSTAVQGLKLGWYWAAQSVSDIFG 3514 Query: 181 GNGNDDIVIGTTHYSAASFVQTGAGLQDKVIGGAFGRSDNLAATVGDFVLGVDKVEVFNQ 240 GNGNDDIVIGTTHYSAASFVQTGAG QDKVI GAFGRSDNLAATVGDFVLGVDKVEVFNQ Sbjct: 3515 GNGNDDIVIGTTHYSAASFVQTGAG-QDKVIVGAFGRSDNLAATVGDFVLGVDKVEVFNQ 3573 Query: 241 SLTQANLSRFVTASAGVNPNDTRLSIDLDGAGPGTLTYTLMLQNVAYNSANTATIFGL 298 SLTQANLSRFVTASAGVNPNDTRLSIDLDGAGPGTLTYTLMLQNVAYNSANTATIFGL Sbjct: 3574 SLTQANLSRFVTASAGVNPNDTRLSIDLDGAGPGTLTYTLMLQNVAYNSANTATIFGL 3631 >gi|300312345|ref|YP_003776437.1| cell wall surface anchor protein [Herbaspirillum seropedicae SmR1] Length = 7175 Score = 219 bits (559), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 142/308 (46%), Positives = 192/308 (62%), Gaps = 18/308 (5%) Query: 1 VNLTVAQSLAPGEHDIVARYTDLAGNTVNGTAQHVSVAGGGDPVTLTDLAVRSSKQAPAA 60 ++LT A SL G+H + ++Y D+AGNTV G +V GG L++L V QA Sbjct: 6876 LSLTPASSLGAGQHTLSSKYIDVAGNTVTGNDIVFNVQGGATAPVLSNLKVSGDGQA--- 6932 Query: 61 VQALNASENRYAVINDL--DTSDISG--HSASGPVISGKVGGGRDSDRYVVTVEMGGKVL 116 QA+N S RYA+++ D++ SG H S +G VG Y+VTV MGGK+L Sbjct: 6933 AQAINDSTTRYAMVSAAAGDSTGSSGLQHQLS---FTGTVGTAGSGHSYLVTVSMGGKLL 6989 Query: 117 AFDEVGAGDFSISLSAGSLAPGLYRDLTVTASRAS-GNEPGQSTAVQGLKLGWYWAAQSV 175 AF++ AGDF + A LAPG Y+DL++ A+ + G GQSTA+ G LGWYWA QS+ Sbjct: 6990 AFNQFAAGDFKLEAPANVLAPGFYKDLSIVATDVTDGPSNGQSTAIGGQTLGWYWAPQSL 7049 Query: 176 SDIFGGNGNDDIVIGTTHYSAASF---VQTGAGLQDKVIGGAFGRSDN--LAATVGDFVL 230 +++ G G+D I +G + + VQTGAG +D ++ GA G +D+ L A V DF++ Sbjct: 7050 TNLTAGAGDDMIPLGAAATANGIYNTEVQTGAG-KDTLVVGASGATDSARLMARVSDFMV 7108 Query: 231 GVDKVEVFNQSLTQANLSRFVTASAGVNPNDTRLSIDLDGAGPGTLTYTLMLQNVAYNSA 290 G+DKV V Q++T ANL RFVTASA N T LSIDLDG GPG+LTYTL+L NV Y + Sbjct: 7109 GMDKVSVAGQTVTAANLDRFVTASAA-PANGTILSIDLDGPGPGSLTYTLLLVNVQYAQS 7167 Query: 291 NTATIFGL 298 NT TIFG+ Sbjct: 7168 NTHTIFGV 7175 >gi|300310420|ref|YP_003774512.1| inner membrane protein [Herbaspirillum seropedicae SmR1] Length = 1882 Score = 210 bits (534), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 129/302 (42%), Positives = 179/302 (59%), Gaps = 7/302 (2%) Query: 1 VNLTVAQSLAPGEHDIVARYTDLAGN--TVNGTAQHVSVAGGGDPVTLTDLAVRSSKQAP 58 V+LT+A+S+ ++ + Y + +GN + N + L+DL V S + Sbjct: 1584 VSLTLAESILGSDNYLYTEYKEASGNIASANEITAYADRPQSVVAPVLSDLNVASPNKG- 1642 Query: 59 AAVQALNASENRYAVINDLDTSDISGHSASGPVISGKVGGGRDSDRYVVTVEMGGKVLAF 118 VQALN S +YA I+D G+ G + GKVG SD+Y++TV+MG KVLAF Sbjct: 1643 -GVQALNDSTTQYASISDPGAPWAPGNYERGLLFGGKVGAEGSSDKYLITVKMGSKVLAF 1701 Query: 119 DEVGAGDFSISLSAGSLAPGLYRDLTVTASR-ASGNEPGQSTAVQGLKLGWYWAAQSVSD 177 D V AGDF++S +A +APGL+ DL+ TA+ SGN G ++ V GLKLG YWA Q + D Sbjct: 1702 DTVNAGDFTLSSAANLIAPGLHEDLSFTATNITSGNNNGMTSTVTGLKLGHYWATQFLGD 1761 Query: 178 IFGGNGNDDIVIGTTHYSAASFVQTGAGLQDKVIGGAFGRSDNLAATVGDFVLGVDKVEV 237 GG+GND+ ++G T + +QT G D + GAFG+ N AAT+ DF G DK+ V Sbjct: 1762 TRGGHGNDNFLLGATKNGLNTLIQTNGG-DDTLTLGAFGKQGNFAATITDFTAGADKIAV 1820 Query: 238 FNQSLTQANLSRFVTASAGVN-PNDTRLSIDLDGAGPGTLTYTLMLQNVAYNSANTATIF 296 F +S+ NL +FV A + N T++ +DLDGA G TYTL LQNVAY ANT T+F Sbjct: 1821 FGKSIDLGNLQQFVKEVAPIQGGNGTKVVVDLDGAAAGNQTYTLHLQNVAYQPANTHTLF 1880 Query: 297 GL 298 G+ Sbjct: 1881 GI 1882 >gi|300310421|ref|YP_003774513.1| hypothetical protein Hsero_1088 [Herbaspirillum seropedicae SmR1] Length = 8016 Score = 203 bits (517), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 126/310 (40%), Positives = 179/310 (57%), Gaps = 14/310 (4%) Query: 1 VNLTVAQSLAPGEHDIVARYTDLAGNTVNGTAQHVSVAGGGDPVTLTDLAVRSSKQAPAA 60 +NL ++ SLAPG H IV++YT G T TA V++ G LTDL V++ + Sbjct: 7709 LNLKISDSLAPGNHAIVSKYTSATGLTSQSTATTVNITGDAKTPLLTDLKVKAGYGEEST 7768 Query: 61 VQALNASENRYAVINDLDTSDISGHSASGPVISGKVG--GGRDSDRYVVTVEMGGKVLAF 118 +A+ + Y I D G G + SG V G +Y+VTV +GGK+L + Sbjct: 7769 AKAILLGGDAYTSITDPGRVGTEGTYDKGLIFSGSVNTPGLTGPQKYLVTVSLGGKLLGY 7828 Query: 119 D--------EVGAGDFSISLSAGSLAPGLYRDLTVTASRASGN--EPGQSTAVQGLKLGW 168 D + F++ ++ LAPGLYRDLT T + + GQ++ ++ LGW Sbjct: 7829 DTFTLSTTDDPATSKFTLQAASNLLAPGLYRDLTATVTNVTEGSIHNGQTSVIKDASLGW 7888 Query: 169 YWAAQSVSDIFGGNGNDDIVIGTTHYSAASFVQTGAGLQDKVIGGAFGRSDNLAATVGDF 228 YW Q + ++ GG GND +++G + ++ V TGAG +G AFG+S NL ATV DF Sbjct: 7889 YWVGQGMGNLIGGQGNDGMLLGASQTTSPFTVTTGAGAGTLTLG-AFGKSSNLTATVTDF 7947 Query: 229 VLGVDKVEVFNQSLTQANLSRFVTASAGVNPNDTRLSIDLDGAGPGTLTYTLMLQNVAYN 288 LG+DKV+V+ QS+T ANLS F +A+A N T L++DLDG G L+YTL LQNVA+N Sbjct: 7948 QLGLDKVQVWGQSITSANLSSFASATASDNGRSTTLTVDLDGVR-GGLSYTLQLQNVAFN 8006 Query: 289 SANTATIFGL 298 +ANT TIFG+ Sbjct: 8007 AANTQTIFGV 8016 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.130 0.367 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 978,198 Number of extensions: 43282 Number of successful extensions: 160 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 4 Length of query: 298 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 213 Effective length of database: 1,217,395 Effective search space: 259305135 Effective search space used: 259305135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 130 (54.7 bits)