BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0190.p (479 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313782|ref|YP_003777874.1| transmembrane protein [Herbaspi... 974 0.0 gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspi... 428 e-121 gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspi... 410 e-116 gi|300310865|ref|YP_003774957.1| response regulator containing a... 172 4e-44 gi|300312843|ref|YP_003776935.1| response regulator containing a... 163 4e-41 >gi|300313782|ref|YP_003777874.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 475 Score = 974 bits (2519), Expect = 0.0, Method: Compositional matrix adjust. Identities = 475/475 (100%), Positives = 475/475 (100%) Query: 5 MTATVSCAVLYQSHRQVERAAIEHGANIARIAQRSISRNVELLTLALDSLTWRYRHTQLH 64 MTATVSCAVLYQSHRQVERAAIEHGANIARIAQRSISRNVELLTLALDSLTWRYRHTQLH Sbjct: 1 MTATVSCAVLYQSHRQVERAAIEHGANIARIAQRSISRNVELLTLALDSLTWRYRHTQLH 60 Query: 65 EMPLQQKMEFLLGENIEARYVAALGIVDTDGELVVGSAALSQILPGPYAQSDFFTAHQTH 124 EMPLQQKMEFLLGENIEARYVAALGIVDTDGELVVGSAALSQILPGPYAQSDFFTAHQTH Sbjct: 61 EMPLQQKMEFLLGENIEARYVAALGIVDTDGELVVGSAALSQILPGPYAQSDFFTAHQTH 120 Query: 125 HAQGDTGLYISAPLTVRLERRMQVVVLSKRLAQPDGSFAGISLMVLYLDYFRDLFQGLTL 184 HAQGDTGLYISAPLTVRLERRMQVVVLSKRLAQPDGSFAGISLMVLYLDYFRDLFQGLTL Sbjct: 121 HAQGDTGLYISAPLTVRLERRMQVVVLSKRLAQPDGSFAGISLMVLYLDYFRDLFQGLTL 180 Query: 185 ENDGVISLYSADGLAYMRIPYNEAFIGSRLNISEDLERIFSPSGPAQGSYFARAQQDGPR 244 ENDGVISLYSADGLAYMRIPYNEAFIGSRLNISEDLERIFSPSGPAQGSYFARAQQDGPR Sbjct: 181 ENDGVISLYSADGLAYMRIPYNEAFIGSRLNISEDLERIFSPSGPAQGSYFARAQQDGPR 240 Query: 245 WLYTFARVPGTGWTVFIGRTGARIFREWYNMLYAVLFLMASFCIASIYLIRRVRVELQRR 304 WLYTFARVPGTGWTVFIGRTGARIFREWYNMLYAVLFLMASFCIASIYLIRRVRVELQRR Sbjct: 241 WLYTFARVPGTGWTVFIGRTGARIFREWYNMLYAVLFLMASFCIASIYLIRRVRVELQRR 300 Query: 305 SALDQQLEELARTDKLTSLLNRRALDEALARAWKRAEENPDAIFSLLFVDVDFFKLYNDT 364 SALDQQLEELARTDKLTSLLNRRALDEALARAWKRAEENPDAIFSLLFVDVDFFKLYNDT Sbjct: 301 SALDQQLEELARTDKLTSLLNRRALDEALARAWKRAEENPDAIFSLLFVDVDFFKLYNDT 360 Query: 365 YGHKAGDYALMAVSRCIVAAVSRLSDQSGRYGGEEFVVLLNGTDTVGATHVAKRIISAIQ 424 YGHKAGDYALMAVSRCIVAAVSRLSDQSGRYGGEEFVVLLNGTDTVGATHVAKRIISAIQ Sbjct: 361 YGHKAGDYALMAVSRCIVAAVSRLSDQSGRYGGEEFVVLLNGTDTVGATHVAKRIISAIQ 420 Query: 425 SLKIPHERSPFDILTVSIGVTSLLREHHSSIEDVVKDADAALYRAKQDGRNKHSL 479 SLKIPHERSPFDILTVSIGVTSLLREHHSSIEDVVKDADAALYRAKQDGRNKHSL Sbjct: 421 SLKIPHERSPFDILTVSIGVTSLLREHHSSIEDVVKDADAALYRAKQDGRNKHSL 475 >gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 428 bits (1101), Expect = e-121, Method: Compositional matrix adjust. Identities = 218/478 (45%), Positives = 313/478 (65%), Gaps = 6/478 (1%) Query: 1 VLALMTATVSCAVLYQSHRQVERAAIEHGANIARIAQRSISRNVELLTLALDSLTWRYRH 60 + LM +S + LYQS++Q ++A+ + +N+ ++QR+ISRN+E+L+L+LD+L +R++H Sbjct: 35 IAVLMVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNLEMLSLSLDTLAYRFQH 94 Query: 61 T-QLHEMPLQQKMEFLLGENIEARYVAALGIVDTDGELVVGSAALSQILPGP-YAQSDFF 118 + Q+ +L G ++A + ++D DG + V S+ L + P YA +F Sbjct: 95 PLSARRLNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQV-SSRLGRGNGAPHYADRAYF 153 Query: 119 TAHQTHHAQGDTGLYISAPLTVRLERRMQVVVLSKRLAQPDGSFAGISLMVLYLDYFRDL 178 AH+ GLY+S P+ L +QVVVLSKRL+ DGSFAG+ +M L L YFR+L Sbjct: 154 IAHRD---SPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGVVVMALDLAYFREL 210 Query: 179 FQGLTLENDGVISLYSADGLAYMRIPYNEAFIGSRLNISEDLERIFSPSGPAQGSYFARA 238 F+GL L ++G+ISLYS +G+AYMR+PY E IG ++ + + +R+ + +GS+FA A Sbjct: 211 FEGLALGDEGIISLYSDEGIAYMRVPYRETIIGRDISQTGNFQRLRAMLSEEEGSFFAHA 270 Query: 239 QQDGPRWLYTFARVPGTGWTVFIGRTGARIFREWYNMLYAVLFLMASFCIASIYLIRRVR 298 DG R LYTF R+PG+ VF+G IF+ W YAVL +A F + + +L+ VR Sbjct: 271 TNDGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAIFGLLTAWLLLHVR 330 Query: 299 VELQRRSALDQQLEELARTDKLTSLLNRRALDEALARAWKRAEENPDAIFSLLFVDVDFF 358 EL+RR ++QLE LARTD LT LLNRRALDEAL W+R + N A FS+LF+DVD+F Sbjct: 331 QELRRRVTAERQLEALARTDGLTDLLNRRALDEALQATWERCQRNLGASFSVLFIDVDYF 390 Query: 359 KLYNDTYGHKAGDYALMAVSRCIVAAVSRLSDQSGRYGGEEFVVLLNGTDTVGATHVAKR 418 K YNDTYGHK GD L V+ + A + R +D +GRYGGEEFVVLL TD GA +A+R Sbjct: 391 KAYNDTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEEFVVLLEQTDLQGAQMMAQR 450 Query: 419 IISAIQSLKIPHERSPFDILTVSIGVTSLLREHHSSIEDVVKDADAALYRAKQDGRNK 476 + A++ I H SP ++T+S+GV +L R+HH +EDV+ ADAALYRAKQ GR++ Sbjct: 451 LCDAVRERHIAHTSSPLAVVTISVGVATLERDHHRRVEDVLNAADAALYRAKQAGRDR 508 >gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 410 bits (1055), Expect = e-116, Method: Compositional matrix adjust. Identities = 215/478 (44%), Positives = 308/478 (64%), Gaps = 5/478 (1%) Query: 1 VLALMTATVSCAVLYQSHRQVERAAIEHGANIARIAQRSISRNVELLTLALDSLTWRYRH 60 ++ +M ++ A LY+ +RQ +AI N+ I QR+ISRN+E+L+LA+D+L +RY+H Sbjct: 14 LIIMMVLSICGASLYEGYRQTRISAITEANNVTLIVQRAISRNMEILSLAMDTLAYRYQH 73 Query: 61 T-QLHEMPLQQKMEFLLGENIEARYVAALGIVDTDGELVVGSA-ALSQILPGPYAQSDFF 118 L +++ +L G A ++ AL I+D DG + V S + S + Y++ +F Sbjct: 74 PLDLTHASDEERYNYLFGSTAAAHHITALAILDADGYVRVTSRRSASPSVVVNYSERTYF 133 Query: 119 TAHQTHHAQGDTGLYISAPLTVRLERRMQVVVLSKRLAQPDGSFAGISLMVLYLDYFRDL 178 T H+ D GL+IS P+ L + VVVLSKRL++ DGSF G+ LM L + YFRDL Sbjct: 134 TVHRD---SADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGVVLMALDMSYFRDL 190 Query: 179 FQGLTLENDGVISLYSADGLAYMRIPYNEAFIGSRLNISEDLERIFSPSGPAQGSYFARA 238 F GLTL DG++SLYS DG+ YMR+PY+ + IG + + + +R+ + G++FARA Sbjct: 191 FTGLTLGEDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPNFQRLRAMLVDNDGNFFARA 250 Query: 239 QQDGPRWLYTFARVPGTGWTVFIGRTGARIFREWYNMLYAVLFLMASFCIASIYLIRRVR 298 DG LYTF R+P V +GR+ IF+ WY+ LY+VL L+ F + L+ VR Sbjct: 251 HTDGVERLYTFRRIPDAPLIVLLGRSERFIFKTWYDTLYSVLLLLIIFTALAGVLLMLVR 310 Query: 299 VELQRRSALDQQLEELARTDKLTSLLNRRALDEALARAWKRAEENPDAIFSLLFVDVDFF 358 EL +R A ++QLE+LARTD LT LLNRR+LDEAL W+R++ +P A FS LF+DVD+F Sbjct: 311 RELLKRVAAEKQLEDLARTDGLTGLLNRRSLDEALLAVWQRSQRSPTAGFSTLFIDVDYF 370 Query: 359 KLYNDTYGHKAGDYALMAVSRCIVAAVSRLSDQSGRYGGEEFVVLLNGTDTVGATHVAKR 418 K YNDTYGH+ GD L AV+ + + R +D +GRYGGEEFVVLL TD GA +A++ Sbjct: 371 KRYNDTYGHEMGDTVLQAVAAVLREHLQRQNDLAGRYGGEEFVVLLEQTDEHGALRMAQQ 430 Query: 419 IISAIQSLKIPHERSPFDILTVSIGVTSLLREHHSSIEDVVKDADAALYRAKQDGRNK 476 + AI + IPH SP +++VSIGV ++ R H +EDV+ AD ALY AK++GRN+ Sbjct: 431 LCEAIYNRGIPHSGSPLGVISVSIGVATMNRAPHQRMEDVLNAADHALYIAKREGRNR 488 >gi|300310865|ref|YP_003774957.1| response regulator containing a CheY-like receiver domain and a GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 554 Score = 172 bits (437), Expect = 4e-44, Method: Compositional matrix adjust. Identities = 134/411 (32%), Positives = 204/411 (49%), Gaps = 30/411 (7%) Query: 85 VAALGIVDTDGELVVGSAALSQILPGPYAQSDFFTAHQTHHAQGDTGLYISAPLTVRLER 144 +A L +VD G V S+ + G Q D+F HQ H + D +I P+ + Sbjct: 116 LAQLTVVDEQGR-TVASSQQRMVQTGSPVQDDYFQYHQ--HRRSDRP-FIGQPMRSHVTG 171 Query: 145 RMQVVVLSKRLAQPDGSFAGISLMVLYLDYFRDLFQGLTLENDGVISLYSADGLAYMRIP 204 + + + +S+R+ DGSFAG+ + + L YF+ ++ + GVI G +R P Sbjct: 172 Q-RTLTVSRRINHVDGSFAGVIVAEIDLAYFQRYYEQFNIGPHGVILFGLTGGSLILRRP 230 Query: 205 YNEAFIGSRLNISEDLERIFSPSGPAQGSYFARAQQDGPRWLYTFARVPGTGWTVFIGRT 264 +SE L + + +GS+ R+++DG + F + G + +F Sbjct: 231 LLADMTDRSPAMSEALRKHMEQA--RRGSFAMRSERDGVERISCFQQ--GEAYPIFTVVA 286 Query: 265 GAR--IFREWYNMLY----AVLFLMASFCIASIYLI----RRVRVELQRRSALDQQLEEL 314 A+ I +W ++ V+ L+A C L+ R R E Q +A Q+LE L Sbjct: 287 LAKDDILEDWSRHVWVRGIGVVLLLAVICFGGKRLVVLVAGRERAEAQAIAAR-QELESL 345 Query: 315 ART-------DKLTSLLNRRALDEALARAWKRAEENPDAIFSLLFVDVDFFKLYNDTYGH 367 RT D LT + NRR D ALA R +++ SL+ +DVD FK +NDTYGH Sbjct: 346 YRTLEAQSQKDGLTDVFNRRYFDAALAAELARLNRTGESL-SLMMIDVDHFKQFNDTYGH 404 Query: 368 KAGDYALMAVSRCIVAAVSRLSDQSGRYGGEEFVVLLNGTDTVGATHVAKRIISAIQSLK 427 AGD L V+ I R D RYGGEEF V+L A +A R++ A++ L+ Sbjct: 405 AAGDVCLRQVAAAISTVARRQGDIVARYGGEEFAVILPNCHPGSAQGLAHRVVQAVRGLE 464 Query: 428 IPHERSPFDILTVSIGVTSLLREHHSSIE--DVVKDADAALYRAKQDGRNK 476 I HERSP ++T+S+GV SL IE +++ AD ALYRAK++GR++ Sbjct: 465 IAHERSPMGVVTISVGVASLASGKSLLIEPRELIDSADGALYRAKEEGRDR 515 >gi|300312843|ref|YP_003776935.1| response regulator containing a CheY-like receiver and a GGDEF domains protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 163 bits (412), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 134/408 (32%), Positives = 207/408 (50%), Gaps = 28/408 (6%) Query: 87 ALGIVDTDGELVVGSAALSQILPGPYAQSDFFTAHQTHHAQGDTGLYISAPLTVRLERRM 146 L + D G+ ++ S+A + P + D+F H+ H D G ++ L R R Sbjct: 112 GLYVFDEHGKRLLTSSA-PPLAPANNSDRDYFIWHRDHD---DVGPHVGKALLSRSTGRW 167 Query: 147 QVVVLSKRLAQPDGSFAGISLMVLYLDYFRDLFQGLTLENDGVISLYSADGLAYMRIPYN 206 ++ +S+RL PDGSFAG+ L L +++F L++ + + G I+L DG R+P++ Sbjct: 168 -IIPMSRRLNHPDGSFAGVLLATLDINHFNQLYRTVEIGKGGSIALLLRDGTLLTRMPFD 226 Query: 207 EAFIGSRLNISEDLERIFSPSGPAQ--GSYFARAQQDGPRWLYTFARVPGTGWTVFIGRT 264 +I N R+F P G + D + ++ V + + Sbjct: 227 PGYI----NRDFSQGRLFLEGLPHSPFGYLEVVSPLDARQRFLSYQAVDKYPLVLVVTLA 282 Query: 265 GARIFREWYNMLY----AVLFLMASFCIASIYLIR----RVRVELQRRSAL------DQQ 310 +W + AVL L+A + L+ R+R E + A+ + + Sbjct: 283 REEALADWKQQTFVYGAAVLLLLAIIGVFGWRLVGQIELRLRAEDRALQAMGELQMANHR 342 Query: 311 LEELARTDKLTSLLNRRALDEALARAWKRAEENPDAIFSLLFVDVDFFKLYNDTYGHKAG 370 LE LA D LT L NRR LD L +KRAE + A +L+ +DVDFFK YND YGH+AG Sbjct: 343 LEMLAHQDGLTGLANRRHLDTMLETEFKRAERS-GAALALIMIDVDFFKQYNDLYGHQAG 401 Query: 371 DYALMAVSRCIVAAVSRLSDQSGRYGGEEFVVLLNGTDTVGATHVAKRIISAIQSLKIPH 430 D L V+ + R D + RYGGEE +LL GTD GA VA+++ + IQ+L++PH Sbjct: 402 DECLRRVAGVLKERQRRPGDLAARYGGEEMAMLLPGTDAEGACAVAEKLRAGIQALELPH 461 Query: 431 ERSPFDILTVSIGVTS--LLREHHSSIEDVVKDADAALYRAKQDGRNK 476 +P ++TVS+GV + LL S++ ++ ADAALY AK +GRN+ Sbjct: 462 RGNPVGVVTVSVGVCALELLPSAARSVQALLGAADAALYEAKHEGRNQ 509 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,466,793 Number of extensions: 61326 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 40 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 40 Length of query: 479 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 390 Effective length of database: 1,198,455 Effective search space: 467397450 Effective search space used: 467397450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 132 (55.5 bits)