BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0187.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herb... 535 e-153 gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [H... 215 3e-57 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 112 4e-26 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 108 4e-25 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 107 1e-24 >gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 535 bits (1377), Expect = e-153, Method: Compositional matrix adjust. Identities = 260/263 (98%), Positives = 261/263 (99%) Query: 1 MTAATTPAFEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALV 60 MTAATTPAFEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALV Sbjct: 1 MTAATTPAFEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALV 60 Query: 61 ASLKDAAIAPRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYW 120 ASLKDA IAPRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYW Sbjct: 61 ASLKDAPIAPRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYW 120 Query: 121 DERLAVNLRHQFFCAQAVLPGMRQRKGGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH 180 DERLAVNLRHQFFCAQAVLPGMR+RK GVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH Sbjct: 121 DERLAVNLRHQFFCAQAVLPGMRERKQGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH 180 Query: 181 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRVDPHDVAALALF 240 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRVDPHDVAALALF Sbjct: 181 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRVDPHDVAALALF 240 Query: 241 LSSDSGAKCTGREYYVDAGWLGA 263 LSSDSGAKCTGREYYVDAGWLGA Sbjct: 241 LSSDSGAKCTGREYYVDAGWLGA 263 >gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 215 bits (548), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 121/251 (48%), Positives = 161/251 (64%), Gaps = 1/251 (0%) Query: 12 ALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALVASLKDAA-IAP 70 A + SL GKRV ITGGG+GIGAA+VEAF QGA V F+DIA E S+AL + A P Sbjct: 11 ASFPSLKGKRVFITGGGTGIGAAIVEAFAQQGAHVAFVDIATEASEALCNEVAAAGHPKP 70 Query: 71 RFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYWDERLAVNLRH 130 F C+L ++ A +AT E++ +G D+L+NNAAND RHK +VT YW++R+A+N R Sbjct: 71 LFRHCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLEEVTLEYWNDRIAINQRP 130 Query: 131 QFFCAQAVLPGMRQRKGGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMARDFGRDG 190 FF Q+V+ GM++R GG I+NF SISWH +Y TAKA G+T G+ARD G Sbjct: 131 SFFAVQSVVEGMKRRGGGSIINFSSISWHQSGGGFPVYTTAKASTLGLTRGLARDLGPHK 190 Query: 191 VRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRVDPHDVAALALFLSSDSGAKCT 250 +RVN + PG + T RQ LW E + I QCL + P +A + LFL++D A CT Sbjct: 191 IRVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWHLARMVLFLAADDSAMCT 250 Query: 251 GREYYVDAGWL 261 +E+ VDAGW+ Sbjct: 251 AQEFIVDAGWV 261 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 112 bits (279), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 81/261 (31%), Positives = 120/261 (45%), Gaps = 4/261 (1%) Query: 1 MTAATTPAFEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALV 60 MT TTP SL GK +ITG GIG AL GA + A A+ Sbjct: 1 MTTKTTPLPAGLQGFSLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVA 60 Query: 61 ASLKDAAIAPRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYW 120 A L+ + + E ++A +IE+ +G + IL+NNA R+ + + W Sbjct: 61 AQLRAEGHTIHESSFDATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDW 120 Query: 121 DERLAVNLRHQFFCAQAVLPGMRQRKGGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH 180 + ++ NL FF AQAV M R G I+N S+ LG P + Y K ++ T Sbjct: 121 HDLISTNLNSVFFAAQAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTK 180 Query: 181 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRV--DPHDVAALA 238 GMA D+G+ G++VN + PG +T L + + ++ P+R D D+ A Sbjct: 181 GMAIDWGKYGIQVNGLGPGYFKTEMNMALVNDAKFTDWLVGRT--PSRRWGDVEDLVGAA 238 Query: 239 LFLSSDSGAKCTGREYYVDAG 259 +FL+SD+ G YVD G Sbjct: 239 VFLASDASRFVNGHILYVDGG 259 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 108 bits (271), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 88/256 (34%), Positives = 123/256 (48%), Gaps = 22/256 (8%) Query: 17 LAGKRVVITGGG--SGIGAALVEAFVGQGAQVCFLDIAAEPSQALVASLKDAAIAPRFFP 74 L K VITGG +G+G A QGA+V LD+A A A + + Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLG---LV 59 Query: 75 CNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYWDERLAVNLRHQFFC 134 ++ + + I G +DIL+NNA KT ++T A +D L V+LR + Sbjct: 60 ADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYM 119 Query: 135 AQAVLPGMRQRKGGVILNFGSISWH-----LGLPDLTLYMTAKAGIEGMTHGMARDFGRD 189 +QAVLP MR ++ G I+ S+S LG P Y AKAG+ G+ MAR+FG + Sbjct: 120 SQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPH---YSAAKAGVLGLARAMAREFGPE 176 Query: 190 GVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPT----RVDPHDVAALALFLSSDS 245 G+RVN+I PG I T ++ EE K A+ +P R D D+ +FL SD Sbjct: 177 GIRVNSITPGLIGTD---IIKGKLTEEKKAEIAETIPLARLGRAD--DIGGACVFLGSDL 231 Query: 246 GAKCTGREYYVDAGWL 261 CTG V+ G L Sbjct: 232 SQYCTGITLDVNGGML 247 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 107 bits (266), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 75/257 (29%), Positives = 119/257 (46%), Gaps = 15/257 (5%) Query: 17 LAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALVASLKDAAIAPRFFPCN 76 L K +ITG SGIG + + QGA+V D+A E ++A + N Sbjct: 3 LKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVAMN 62 Query: 77 LMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYWDERLAVNLRHQFFCAQ 136 + + + + GG+DI+I+NA A+++ W + LA++L F + Sbjct: 63 VTDETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLTTR 122 Query: 137 AVLPGM-RQRKGGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMARDFGRDGVRVNA 195 A + M +Q +GG I+ GS+ HL P Y+TAK G+ G+ +A++ +D +R N Sbjct: 123 AAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRTNV 182 Query: 196 IIPGAIRTPRQTLLWHTPEE-------EAKILAAQCLPTRVD-----PHDVAALALFLSS 243 I PG +RTP + PE+ E ++ L VD DVA A+FL++ Sbjct: 183 ICPGFVRTP--LVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFLAA 240 Query: 244 DSGAKCTGREYYVDAGW 260 TG+ V GW Sbjct: 241 FPTNALTGQSVVVSHGW 257 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 892,464 Number of extensions: 38792 Number of successful extensions: 289 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 45 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)